pycom13g18920

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0021282
Physical Location & Seq
Reverse (-)
14945473 .. 14945948
476 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g18920.1

Sequence Viewer

Length: 276 bp
ATGGTGAGGTCCCAAATGAACTTCAAGAGGCTTTCTCTCACTGACATCAAGATTGATATCAAGAGGGTCCCCAAGAAGAAGGAATTGCTTGCCGCAATGGAAGCTGCTGATGTGAAGAAGAAGTGGGAGAACAGCTCGTGGGGTAGGAAACTGATTGTTCAGAAGAGAAGAGCAGCTCTCAACGACTTTGACCGATTCAAGCTCATGTTGGCTAAGATTAAGAGGGCCGGACTGGTGAGGAGCGAGCTTGCAAAATTGAAAAAGTCCACAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L14e

Protein Analysis

92

Amino Acids

10.58

Weight (kDa)

10.95

Isoelectric Point (pI)

31.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14e PF01929 3 - 75 8.1e-27 Ribosomal protein L14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021282)

Species Orthologous Gene IDs
pyrus_communis pycom13g18920
rosa_multiflora Rmu_co8287205.1_g000001 Rmu_co8313979.1_g000001
rosa_samantha Rh4CG054000 Rh4CG057600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 93
AgsI TTSAA 3 cut(s) 25, 199, 259
AluBI AGCT 6 cut(s) 104, 135, 176, 202, 247, 272
AluI AGCT 6 cut(s) 104, 135, 176, 202, 247, 272
AoxI GGCC 1 cut(s) 225
ApeKI GCWGC 2 cut(s) 104, 173
AspS9I GGNCC 3 cut(s) 9, 67, 225
AsuHPI GGTGA 2 cut(s) 16, 247
AvaII GGWCC 2 cut(s) 9, 67
BauI CACGAG 1 cut(s) 136
BbvI GCAGC 2 cut(s) 91, 185
BisI GCNGC 3 cut(s) 93, 105, 174
BlsI GCNGC 3 cut(s) 94, 106, 175
Bme18I GGWCC 2 cut(s) 9, 67
BmgT120I GGNCC 3 cut(s) 9, 67, 225
BmiI GGNNCC 3 cut(s) 11, 68, 69
BplI GAGNNNNNCTC 6 cut(s) 19, 51, 119, 151, 162, 194
BsaBI GATNNNNATC 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 56
BseJI GATNNNNATC 1 cut(s) 56
BseMI GCAATG 1 cut(s) 102
BseNI ACTGG 1 cut(s) 237
BseRI GAGGAG 1 cut(s) 253
BseXI GCAGC 2 cut(s) 91, 185
BshFI GGCC 1 cut(s) 227
BsiSI CCGG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 1 cut(s) 227
BspACI CCGC 1 cut(s) 93
BspANI GGCC 1 cut(s) 227
BspLI GGNNCC 3 cut(s) 11, 68, 69
BspQI GCTCTTC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 102
BsrI ACTGG 1 cut(s) 237
BssSI CACGAG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 136
Bst6I CTCTTC 2 cut(s) 158, 163
BstC8I GCNNGC 3 cut(s) 90, 245, 249
BstDEI CTNAG 1 cut(s) 213
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstV1I GCAGC 2 cut(s) 91, 185
BsuRI GGCC 1 cut(s) 227
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 3 cut(s) 90, 245, 249
Cfr13I GGNCC 3 cut(s) 9, 67, 225
CviAII CATG 1 cut(s) 205
CviJI RGCY 9 cut(s) 31, 104, 135, 176, 202, 212, 227, 247, 272
CviKI_1 RGCY 9 cut(s) 31, 104, 135, 176, 202, 212, 227, 247, 272
DdeI CTNAG 1 cut(s) 213
Eam1104I CTCTTC 2 cut(s) 158, 163
EarI CTCTTC 2 cut(s) 158, 163
Eco32I GATATC 1 cut(s) 58
Eco47I GGWCC 2 cut(s) 9, 67
EcoO109I RGGNCCY 2 cut(s) 9, 67
EcoRV GATATC 1 cut(s) 58
FaeI CATG 1 cut(s) 208
FaiI YATR 1 cut(s) 206
FaqI GGGAC 1 cut(s) 53
FatI CATG 1 cut(s) 204
Fnu4HI GCNGC 3 cut(s) 93, 105, 174
Fsp4HI GCNGC 3 cut(s) 93, 105, 174
GluI GCNGC 3 cut(s) 93, 105, 174
HaeIII GGCC 1 cut(s) 227
HapII CCGG 1 cut(s) 228
Hin1II CATG 1 cut(s) 208
HinfI GANTC 1 cut(s) 195
HpaII CCGG 1 cut(s) 228
HphI GGTGA 2 cut(s) 16, 247
Hpy166II GTNNAC 1 cut(s) 267
Hpy188I TCNGA 1 cut(s) 162
Hpy188III TCNNGA 3 cut(s) 25, 49, 61
Hpy8I GTNNAC 1 cut(s) 267
HpyAV CCTTC 1 cut(s) 73
HpyCH4V TGCA 1 cut(s) 251
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpyF3I CTNAG 1 cut(s) 213
Hsp92II CATG 1 cut(s) 208
KflI GGGWCCC 1 cut(s) 67
LguI GCTCTTC 1 cut(s) 163
LmnI GCTCC 1 cut(s) 240
LpnPI CCDG 2 cut(s) 218, 241
Lsp1109I GCAGC 2 cut(s) 91, 185
MboII GAAGA 5 cut(s) 88, 127, 130, 175, 180
MluCI AATT 2 cut(s) 83, 254
MnlI CCTC 4 cut(s) 21, 57, 216, 231
MseI TTAA 2 cut(s) 219, 274
MspI CCGG 1 cut(s) 228
MwoI GCNNNNNNNGC 1 cut(s) 101
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 3 cut(s) 11, 68, 69
PciSI GCTCTTC 1 cut(s) 163
PfeI GAWTC 1 cut(s) 195
PkrI GCNGC 3 cut(s) 94, 106, 175
PpuMI RGGWCCY 2 cut(s) 9, 67
Psp5II RGGWCCY 2 cut(s) 9, 67
PspN4I GGNNCC 3 cut(s) 11, 68, 69
PspPI GGNCC 3 cut(s) 9, 67, 225
PspPPI RGGWCCY 2 cut(s) 9, 67
SapI GCTCTTC 1 cut(s) 163
SaqAI TTAA 2 cut(s) 219, 274
SatI GCNGC 3 cut(s) 93, 105, 174
Sau96I GGNCC 3 cut(s) 9, 67, 225
SetI ASST 7 cut(s) 11, 106, 137, 178, 204, 249, 274
SinI GGWCC 2 cut(s) 9, 67
Sse9I AATT 2 cut(s) 83, 254
SsiI CCGC 1 cut(s) 93
TaqII GACCGA 1 cut(s) 207
TasI AATT 2 cut(s) 83, 254
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 1 cut(s) 195
Tru1I TTAA 2 cut(s) 219, 274
Tru9I TTAA 2 cut(s) 219, 274
TscAI CASTG 1 cut(s) 46
TseI GCWGC 2 cut(s) 104, 173
TspDTI ATGAA 1 cut(s) 32
TspRI CASTG 1 cut(s) 46
VpaK11BI GGWCC 2 cut(s) 9, 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.