pycom13g20210
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012839
Physical Location & Seq
Reverse (-)
16216624 .. 16220488
3865 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3207 bp
ATGCAGGATGGATTTGATTTTTTGGGAAATTGGAACTCCTCTGATCAAACACCTTGCGGATGGATGGGTGTAAATTGCTCTTCTGGTTACGATCCAGTGGTTAAAGGTCTTGATTTGAGTTCCCTGAATCTTTCGGGAGTCCTCAGTCCTAGCATTGGTGGTTTGGTCCACCTAACTTCTCTTGATCTTTCAGACAATGGTTTCATGGGAGGTATTCCTAAGGAGATAGGAAACTGTTGGAGTTTGGAAAGAATTTATCTGAACAATAACAAGTTCACTGGCCAAATACCTTTGGAAGTGGGTAAGCTGTTTAATTTAACAGTTTTGAATCTTTGTAATAACAGAATCAGTGGTCCGATTCCAGATGAGATTGGGAACTTGTCTTCACTGGCTGAGTTCGTGGTATACACCAACAATATTACTGGTTCAATACCTCATTCCCTTGGGAATCTCAAGAATCTGGTAACGTTTAGAGCTGGGCAGAATGCTATTTCTGGTAGCCTACCGGCCGAAATAGGTAGATGTGAGAGCTTGCAATTGCTTGGTCTCGCCCAAAATTCTATAGGAGGGGAATTGCCTAAGGAGTTTGGGATGCTTCAAAGCTTGACAGATGTGGTTCTGTGGGGTAACCAGGTATCAGGATTTATACCCAAGGAGCTTGGAAATTGCTCTAGCCTTGAGACTATTGCTTTGTATCAGAACAATCTTGTGGGGCCTTTACCTCCAGAAATTGGGAACCTCAAGTCATTGAGGAAATTGTACATTTACAGGAATGGCTTGAATGGAACCATTCCAAGGGAATTTGGAAATCTTTCTTTGGCATCTGAGATTGATTTCTCGGAGAACTATTTGACAGGTGAGATCCCATCAGAGATAAGTAAGATAAAGGGTCTAACCTTACTGTACCTGTTCCAGAACCAGCTTACGGGTGTGATTCCAAATGAACTGAGTGGCTTGGGGAACCTGACTAAGCTTGACCTTTCCATTAATAATCTCAGAGGTCCTATCCCCGATGGGTTTCAATATTTGACTCAAATGTACCAGTTACAACTGTTCGACAATTCTCTAAGCGGTAGCATACCACGGGGGCTTGGACTTCACAGCCGACTGTGGGTAGTTGATTTATCCGATAACTTACTGACAGGAAGAATACCTCCATATATTTGTCGATATTCCAATCTAATTTTGTTGAACCTGGAGGCTAATGATCTGATCGGAAATATTCCGGCTGGAGTCTTGAACTGCAAGTCATTGGTGCAACTTCGTCTTGTTGGAAACATGCTTACTGGGAGCTTTCCTTCGCAACTATGCAGTTTGCCAAACCTTTCTGCAATTGAGTTGGATCAGAACAAATTCACTGGTCCAATTCCTCCAGAGATTAGGAACTGCCAAAAGTTGCAAAGGCTTCATATTTCAGACAACTACTTTACGTCTGAGTTGCCAAAGGAAATAGGATATCTGTCTCAACTGGTGACTTTCAACACGTCATCCAATTTGTTCACTGGGCGGATTCCACCAGAAATCGTTAACTGCAAGATGCTTCAACGACTTGATCTCAGTCGAAATAGGTTCATGGGTGCTTTGCCAAATGAGCTGGGAACACTTCTGCAACTAGAGCTTCTCAGGCTTTCAGAAAATAATTTCACGGGAAACATACCAGCAGAATTAGGAAACCTCTCCCATTTGACTGAGTTGCAGATGGGTGCCAACTTGTTTTCTGGTGAAATACCACCAGAATTGGGTTCTATTTCAAGCTTGCAGATTGCAATGAATCTGAGCTTTAATAATTTCAGTGGGAGAATACCGCCAGCTCTTGGAAATCTTAATATGCTTGAATTCCTCTTGCTCAATAACAACCATTTAACTGGTGAAATTCCTAGCACATTTGAGAGTCTGTCAAGCTTACTAGGGTGCAACTTCTCATACAATGACCTTACTGGACCTTTACCTTCCATCCCATTGTTTCAGAACATGGAGATTAGCAGCTTCATCGGTAACAAAGGGCTCTGTGGCAGACCGCTTGGTGGTTGCAATGTTAATTCATCTCCTCAATCTGTTCCATCTCTGGCAAGTGGGGGTACTCAACGCAGTAAAATTGTTACAGTAATTGCTGCTGCTGTGGGTGGCGTTTCTCTTATTCTAATTGCTGTTATCTTATATTTCATGAGACATCCAGGCCAGAGAGTTCCTTCCTTGCAAGATAAGGATGCTCTATCTCCAGATACGGATATGTACTTACCTCCAAAGGAGGGATTTATGTTCCAAGATCTAGTTGAGGCGACAAATAACTTTCATGAAAGCTATGTTATTGGAAGGGGAGCTTGTGGAATTGTGTATAAGGCAGTAATGCGAACTGGACAGACTATTGCTGTTAAGAAATTGTCATCTAACCGAGAGAGCAATAACATTGAAAACAGTTTTCAGGCCGAGATTAAGACCTTAGGAAATATAAGGCATCGGAATATTGTGAAGCTATATGGATTCTGCTATCACCAGGGTTCCAATCTACTTCTTTATGAGTACATGGCAAAGGGTAGCTTGGGTGAATTGCTTCATGGTGACTCTTGTAGCTTGGACTGGCCAATGCGTTTTATGATTGCTTTGGGAGCTGCCGAAGGTCTTGCTTATTTACATCATGACTGCAAACCGAAGATTGTCCACCGTGATATAAAGTCCAACAATATTTTGCTAGATGAGAACTTTGAAGCCCATGTTGGTGATTTTGGTTTGGCCAAAGTGATTGATATGCCTTATTCAAAATCAATGTCAGCAGTTGCAGGGTCGTATGGATATATTGCTCCTGAGTACGCATACACCATGAAGGTTACAGAAAAATGCGACATCTATAGCTATGGGGTTGTTCTGTTGGAGTTGCTAACTGGAAGAACTCCTGTCCAATCACTAGATCAAGGAGGCGACTTGGTCACCTGGGTGAGACACTATGTTCAAGACCATTCATTGACATCTGGGATACTCGATAGTCGGTTAAATCTTCTAGATGGACTTCTTGTTGATCACATGCTTAATGTCTTAAAAATTGCTTTGATATGCACAAGTATGTCCCCTTTTGACCGTCCATCGATTCGAGAAGTTGTCTTGATGCTTATTGAGTCGAATGAGCAAGATGGGGACTTTCCTCCGTCTCCAACTTTCAATCTTCCTTTGAAAGATGCAACGGATCCTCCTTTGAAAGATGATAGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1069

Amino Acids

116.83

Weight (kDa)

5.33

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 37 - 157 6.1e-10 Leucine-rich repeat region
LRR_14 PF23598 166 - 257 4.9e-08 Leucine-rich repeat region
LRR_14 PF23598 503 - 587 9.1e-07 Leucine-rich repeat region
Pkinase PF00069 765 - 976 3.2e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 769 - 970 1.6e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012839)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33170 AT2G33170
fragaria_vesca FvH4_4g05590 FvH4_4g05590 FvH4_4g05590 FvH4_4g05590 FvH4_4g05590
malus_domestica MD16G1234300.v1.1
prunus_persica Prupe.1G057600_v2.0.a1
pyrus_communis pycom13g20210
rosa_chinensis RchiOBHm_Chr4g0396751
rosa_laevigata RLG00000009506
rosa_multiflora Rmu_sc0000487.1_g000004 Rmu_sc0003840.1_g000008
rosa_roxburghii Rroxscaffold_5G00341590
rosa_rugosa Rorug03G0347700
rosa_samantha Rh4BG069400
rosa_wichuraiana Rw4G005710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1703
AccB7I CCANNNNNTGG 2 cut(s) 731, 1814
AccI GTMKAC 1 cut(s) 405
AciI CCGC 5 cut(s) 57, 1071, 1507, 1805, 2018
AclI AACGTT 1 cut(s) 467
AclWI GGATC 5 cut(s) 86, 856, 1350, 3173, 3186
AcoI YGGCCR 4 cut(s) 280, 507, 2579, 2730
AcsI RAATTY 6 cut(s) 252, 556, 800, 1352, 1835, 1872
AfaI GTAC 7 cut(s) 761, 905, 1040, 2080, 2234, 2522, 2807
AfiI CCNNNNNNNGG 9 cut(s) 155, 731, 795, 925, 1111, 1739, 1814, 2024, 2249
AflIII ACRYGT 1 cut(s) 1482
AjiI CACGTC 1 cut(s) 1485
AjnI CCWGG 5 cut(s) 630, 1194, 2173, 2493, 2927
AjuI GAANNNNNNNTTGG 2 cut(s) 2697, 2729
AleI CACNNNNGTG 1 cut(s) 2930
Alw26I GTCTC 6 cut(s) 551, 674, 1467, 2161, 2929, 3147
AlwI GGATC 5 cut(s) 86, 856, 1350, 3173, 3186
AoxI GGCC 7 cut(s) 280, 507, 713, 2176, 2424, 2579, 2730
ApeKI GCWGC 4 cut(s) 1983, 2111, 2114, 2609
ApoI RAATTY 6 cut(s) 252, 556, 800, 1352, 1835, 1872
ArsI GACNNNNNNTTYG 4 cut(s) 673, 705, 2750, 2782
AseI ATTAAT 1 cut(s) 987
Asp700I GAANNNNTTC 3 cut(s) 811, 1352, 2550
AspS9I GGNCC 6 cut(s) 166, 353, 713, 1001, 1361, 1940
AvaII GGWCC 5 cut(s) 166, 353, 1001, 1361, 1940
AxyI CCTNAGG 3 cut(s) 219, 579, 2440
BalI TGGCCA 3 cut(s) 282, 2581, 2732
BamHI GGATCC 1 cut(s) 3178
BanI GGYRCC 1 cut(s) 1703
BanII GRGCYC 1 cut(s) 2007
BbsI GAAGAC 1 cut(s) 375
BbvI GCAGC 4 cut(s) 1995, 2098, 2101, 2596
BcgI CGANNNNNNTGC 4 cut(s) 1972, 2006, 2603, 2637
BciT130I CCWGG 5 cut(s) 632, 1196, 2175, 2495, 2929
BciVI GTATCC 1 cut(s) 2964
BclI TGATCA 2 cut(s) 43, 3013
BcoDI GTCTC 6 cut(s) 551, 674, 1467, 2161, 2929, 3147
BfaI CTAG 9 cut(s) 150, 672, 1613, 1878, 1907, 2270, 2690, 2903, 2996
BfmI CTRYAG 2 cut(s) 561, 2845
BfuI GTATCC 1 cut(s) 2964
BglII AGATCT 1 cut(s) 2266
BisI GCNGC 4 cut(s) 1984, 2112, 2115, 2610
BlsI GCNGC 4 cut(s) 1985, 2113, 2116, 2611
Bme1390I CCNGG 5 cut(s) 632, 1196, 2175, 2495, 2929
Bme18I GGWCC 5 cut(s) 166, 353, 1001, 1361, 1940
BmgBI CACGTC 1 cut(s) 1485
BmgT120I GGNCC 6 cut(s) 166, 353, 713, 1001, 1361, 1940
BmiI GGNNCC 7 cut(s) 714, 737, 787, 962, 1705, 2500, 3180
BmrFI CCNGG 5 cut(s) 632, 1196, 2175, 2495, 2929
BmrI ACTGGG 2 cut(s) 1296, 1512
BmsI GCATC 7 cut(s) 582, 830, 1527, 2197, 2464, 3090, 3160
BmuI ACTGGG 2 cut(s) 1296, 1512
BpiI GAAGAC 1 cut(s) 375
BpmI CTGGAG 5 cut(s) 708, 1217, 1251, 1356, 2202
BpuEI CTTGAG 3 cut(s) 437, 698, 725
Bsa29I ATCGAT 1 cut(s) 3080
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 6 cut(s) 442, 651, 794, 1082, 2494, 2928
BsaXI ACNNNNNCTCC 2 cut(s) 3166, 3196
Bsc4I CCNNNNNNNGG 9 cut(s) 155, 731, 795, 925, 1111, 1739, 1814, 2024, 2249
Bse118I RCCGGY 1 cut(s) 505
Bse21I CCTNAGG 3 cut(s) 219, 579, 2440
Bse3DI GCAATG 2 cut(s) 1773, 2038
BseBI CCWGG 5 cut(s) 632, 1196, 2175, 2495, 2929
BseCI ATCGAT 1 cut(s) 3080
BseDI CCNNGG 6 cut(s) 442, 651, 794, 1082, 2494, 2928
BseGI GGATG 8 cut(s) 13, 65, 69, 597, 1487, 1953, 2170, 2212
BseLI CCNNNNNNNGG 9 cut(s) 155, 731, 795, 925, 1111, 1739, 1814, 2024, 2249
BseMI GCAATG 2 cut(s) 1773, 2038
BseRI GAGGAG 2 cut(s) 28, 2037
BseX3I CGGCCG 1 cut(s) 507
BseXI GCAGC 4 cut(s) 1995, 2098, 2101, 2596
BseYI CCCAGC 2 cut(s) 476, 1594
Bsh1285I CGRYCG 1 cut(s) 510
BshFI GGCC 7 cut(s) 282, 509, 715, 2178, 2426, 2581, 2732
BshNI GGYRCC 1 cut(s) 1703
BshVI ATCGAT 1 cut(s) 3080
BsiEI CGRYCG 1 cut(s) 510
BsiSI CCGG 2 cut(s) 506, 1226
BslFI GGGAC 2 cut(s) 3046, 3143
BslI CCNNNNNNNGG 9 cut(s) 155, 731, 795, 925, 1111, 1739, 1814, 2024, 2249
BsmAI GTCTC 6 cut(s) 551, 674, 1467, 2161, 2929, 3147
BsmBI CGTCTC 1 cut(s) 3147
BsmFI GGGAC 2 cut(s) 3046, 3143
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 7 cut(s) 282, 509, 715, 2178, 2426, 2581, 2732
Bso31I GGTCTC 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 2007
Bsp1407I TGTACA 1 cut(s) 759
BspACI CCGC 5 cut(s) 57, 1071, 1507, 1805, 2018
BspANI GGCC 7 cut(s) 282, 509, 715, 2178, 2426, 2581, 2732
BspDI ATCGAT 1 cut(s) 3080
BspHI TCATGA 3 cut(s) 2163, 2293, 2635
BspLI GGNNCC 7 cut(s) 714, 737, 787, 962, 1705, 2500, 3180
BspPI GGATC 5 cut(s) 86, 856, 1350, 3173, 3186
BspQI GCTCTTC 1 cut(s) 85
BspT107I GGYRCC 1 cut(s) 1703
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 2 cut(s) 1773, 2038
BsrFI RCCGGY 1 cut(s) 505
BsrGI TGTACA 1 cut(s) 759
BssAI RCCGGY 1 cut(s) 505
BssECI CCNNGG 6 cut(s) 442, 651, 794, 1082, 2494, 2928
BssNAI GTATAC 1 cut(s) 406
BssT1I CCWWGG 3 cut(s) 442, 651, 794
Bst1107I GTATAC 1 cut(s) 406
Bst2UI CCWGG 5 cut(s) 632, 1196, 2175, 2495, 2929
Bst4CI ACNGT 9 cut(s) 236, 322, 903, 1053, 1110, 2104, 2417, 2663, 3074
Bst6I CTCTTC 1 cut(s) 85
BstAUI TGTACA 1 cut(s) 759
BstC8I GCNNGC 3 cut(s) 533, 1757, 1809
BstDSI CCRYGG 1 cut(s) 1082
BstEII GGTNACC 2 cut(s) 626, 2923
BstF5I GGATG 8 cut(s) 13, 65, 69, 597, 1487, 1953, 2170, 2212
BstMAI GTCTC 6 cut(s) 551, 674, 1467, 2161, 2929, 3147
BstMCI CGRYCG 1 cut(s) 510
BstMWI GCNNNNNNNGC 4 cut(s) 1591, 1615, 1624, 2606
BstNI CCWGG 5 cut(s) 632, 1196, 2175, 2495, 2929
BstNSI RCATGY 2 cut(s) 1282, 3022
BstPI GGTNACC 2 cut(s) 626, 2923
BstSCI CCNGG 5 cut(s) 630, 1194, 2173, 2493, 2927
BstSFI CTRYAG 2 cut(s) 561, 2845
BstV1I GCAGC 4 cut(s) 1995, 2098, 2101, 2596
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 3 cut(s) 861, 2266, 3178
BstXI CCANNNNNNTGG 1 cut(s) 1865
BstYI RGATCY 3 cut(s) 861, 2266, 3178
BstZ17I GTATAC 1 cut(s) 406
BstZI CGGCCG 1 cut(s) 507
Bsu15I ATCGAT 1 cut(s) 3080
Bsu36I CCTNAGG 3 cut(s) 219, 579, 2440
BsuI GTATCC 1 cut(s) 2964
BsuRI GGCC 7 cut(s) 282, 509, 715, 2178, 2426, 2581, 2732
BsuTUI ATCGAT 1 cut(s) 3080
BtgI CCRYGG 1 cut(s) 1082
BtrI CACGTC 1 cut(s) 1485
BtsCI GGATG 8 cut(s) 13, 65, 69, 597, 1487, 1953, 2170, 2212
BtsIMutI CAGTG 7 cut(s) 102, 276, 355, 386, 1356, 1500, 1798
Cac8I GCNNGC 3 cut(s) 533, 1757, 1809
CciI TCATGA 3 cut(s) 2163, 2293, 2635
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 6 cut(s) 166, 353, 713, 1001, 1361, 1940
ClaI ATCGAT 1 cut(s) 3080
CsiI ACCWGGT 1 cut(s) 630
Csp6I GTAC 7 cut(s) 760, 904, 1039, 2079, 2233, 2521, 2806
CspCI CAANNNNNGTGG 2 cut(s) 1719, 1754
CviQI GTAC 7 cut(s) 760, 904, 1039, 2079, 2233, 2521, 2806
EaeI YGGCCR 4 cut(s) 280, 507, 2579, 2730
EagI CGGCCG 1 cut(s) 507
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
EciI GGCGGA 1 cut(s) 1522
EclXI CGGCCG 1 cut(s) 507
Eco130I CCWWGG 3 cut(s) 442, 651, 794
Eco24I GRGCYC 1 cut(s) 2007
Eco31I GGTCTC 1 cut(s) 551
Eco32I GATATC 1 cut(s) 1457
Eco47I GGWCC 5 cut(s) 166, 353, 1001, 1361, 1940
Eco52I CGGCCG 1 cut(s) 507
Eco81I CCTNAGG 3 cut(s) 219, 579, 2440
Eco91I GGTNACC 2 cut(s) 626, 2923
EcoO109I RGGNCCY 2 cut(s) 713, 1001
EcoO65I GGTNACC 2 cut(s) 626, 2923
EcoRI GAATTC 1 cut(s) 1835
EcoRII CCWGG 5 cut(s) 630, 1194, 2173, 2493, 2927
EcoRV GATATC 1 cut(s) 1457
EcoT14I CCWWGG 3 cut(s) 442, 651, 794
EcoT38I GRGCYC 1 cut(s) 2007
ErhI CCWWGG 3 cut(s) 442, 651, 794
Esp3I CGTCTC 1 cut(s) 3147
FalI AAGNNNNNCTT 4 cut(s) 2305, 2337, 2522, 2554
FaqI GGGAC 2 cut(s) 3046, 3143
FbaI TGATCA 2 cut(s) 43, 3013
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 4 cut(s) 1984, 2112, 2115, 2610
FokI GGATG 8 cut(s) 20, 72, 76, 604, 1474, 1940, 2157, 2219
FriOI GRGCYC 1 cut(s) 2007
Fsp4HI GCNGC 4 cut(s) 1984, 2112, 2115, 2610
FspBI CTAG 9 cut(s) 150, 672, 1613, 1878, 1907, 2270, 2690, 2903, 2996
GluI GCNGC 4 cut(s) 1984, 2112, 2115, 2610
GsaI CCCAGC 2 cut(s) 480, 1598
GsuI CTGGAG 5 cut(s) 708, 1217, 1251, 1356, 2202
HaeIII GGCC 7 cut(s) 282, 509, 715, 2178, 2426, 2581, 2732
HapII CCGG 2 cut(s) 506, 1226
HincII GTYRAC 1 cut(s) 1528
HindII GTYRAC 1 cut(s) 1528
HindIII AAGCTT 4 cut(s) 601, 971, 1753, 1900
HpaI GTTAAC 1 cut(s) 1528
HpaII CCGG 2 cut(s) 506, 1226
Hpy166II GTNNAC 6 cut(s) 169, 276, 406, 1500, 1528, 2659
Hpy8I GTNNAC 6 cut(s) 169, 276, 406, 1500, 1528, 2659
HpyAV CCTTC 6 cut(s) 1308, 1959, 2199, 2307, 2609, 2815
HpyCH4III ACNGT 9 cut(s) 236, 322, 903, 1053, 1110, 2104, 2417, 2663, 3074
HpyCH4IV ACGT 3 cut(s) 467, 1430, 1484
HpyF10VI GCNNNNNNNGC 4 cut(s) 1591, 1615, 1624, 2606
HpySE526I ACGT 3 cut(s) 467, 1430, 1484
Ksp22I TGATCA 2 cut(s) 43, 3013
KspAI GTTAAC 1 cut(s) 1528
LguI GCTCTTC 1 cut(s) 85
LmnI GCTCC 5 cut(s) 655, 1290, 2318, 2606, 2803
Lsp1109I GCAGC 4 cut(s) 1995, 2098, 2101, 2596
LweI GCATC 7 cut(s) 582, 830, 1527, 2197, 2464, 3090, 3160
MabI ACCWGGT 1 cut(s) 630
MaeI CTAG 9 cut(s) 150, 672, 1613, 1878, 1907, 2270, 2690, 2903, 2996
MaeII ACGT 3 cut(s) 467, 1430, 1484
MboII GAAGA 7 cut(s) 72, 375, 1158, 2662, 2895, 2984, 3149
MfeI CAATTG 2 cut(s) 536, 1332
MflI RGATCY 3 cut(s) 861, 2266, 3178
MhlI GDGCHC 1 cut(s) 2007
MlsI TGGCCA 3 cut(s) 282, 2581, 2732
MluNI TGGCCA 3 cut(s) 282, 2581, 2732
MlyI GAGTC 6 cut(s) 147, 1024, 1242, 1900, 2555, 3119
MmeI TCCRAC 6 cut(s) 218, 1252, 1320, 2700, 2847, 3170
Mox20I TGGCCA 3 cut(s) 282, 2581, 2732
MroXI GAANNNNTTC 3 cut(s) 811, 1352, 2550
MscI TGGCCA 3 cut(s) 282, 2581, 2732
MslI CAYNNNNRTG 3 cut(s) 2715, 2930, 3056
Msp20I TGGCCA 3 cut(s) 282, 2581, 2732
MspI CCGG 2 cut(s) 506, 1226
MspR9I CCNGG 5 cut(s) 632, 1196, 2175, 2495, 2929
MunI CAATTG 2 cut(s) 536, 1332
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 5 cut(s) 632, 1196, 2175, 2495, 2929
MwoI GCNNNNNNNGC 4 cut(s) 1591, 1615, 1624, 2606
NlaIV GGNNCC 7 cut(s) 714, 737, 787, 962, 1705, 2500, 3180
NmeAIII GCCGAG 1 cut(s) 2452
NmuCI GTSAC 3 cut(s) 1471, 2558, 2923
NspI RCATGY 2 cut(s) 1282, 3022
OliI CACNNNNGTG 1 cut(s) 2930
PagI TCATGA 3 cut(s) 2163, 2293, 2635
PciSI GCTCTTC 1 cut(s) 85
PctI GAATGC 1 cut(s) 490
PdmI GAANNNNTTC 3 cut(s) 811, 1352, 2550
PflFI GACNNNGTC 1 cut(s) 2921
PflMI CCANNNNNTGG 2 cut(s) 731, 1814
PkrI GCNGC 4 cut(s) 1985, 2113, 2116, 2611
PleI GAGTC 6 cut(s) 146, 1024, 1241, 1899, 2555, 3118
PpsI GAGTC 6 cut(s) 146, 1024, 1241, 1899, 2555, 3118
PpuMI RGGWCCY 1 cut(s) 1001
PshBI ATTAAT 1 cut(s) 987
Psp1406I AACGTT 1 cut(s) 467
Psp5II RGGWCCY 1 cut(s) 1001
Psp6I CCWGG 5 cut(s) 630, 1194, 2173, 2493, 2927
PspEI GGTNACC 2 cut(s) 626, 2923
PspFI CCCAGC 2 cut(s) 476, 1594
PspGI CCWGG 5 cut(s) 630, 1194, 2173, 2493, 2927
PspN4I GGNNCC 7 cut(s) 714, 737, 787, 962, 1705, 2500, 3180
PspPI GGNCC 6 cut(s) 166, 353, 713, 1001, 1361, 1940
PspPPI RGGWCCY 1 cut(s) 1001
PsuI RGATCY 3 cut(s) 861, 2266, 3178
PsyI GACNNNGTC 1 cut(s) 2921
RsaI GTAC 7 cut(s) 761, 905, 1040, 2080, 2234, 2522, 2807
RsaNI GTAC 7 cut(s) 760, 904, 1039, 2079, 2233, 2521, 2806
RseI CAYNNNNRTG 3 cut(s) 2715, 2930, 3056
SapI GCTCTTC 1 cut(s) 85
SatI GCNGC 4 cut(s) 1984, 2112, 2115, 2610
Sau96I GGNCC 6 cut(s) 166, 353, 713, 1001, 1361, 1940
SchI GAGTC 6 cut(s) 147, 1024, 1242, 1900, 2555, 3119
ScrFI CCNGG 5 cut(s) 632, 1196, 2175, 2495, 2929
SduI GDGCHC 1 cut(s) 2007
SexAI ACCWGGT 1 cut(s) 630
SfaNI GCATC 7 cut(s) 582, 830, 1527, 2197, 2464, 3090, 3160
SfcI CTRYAG 2 cut(s) 561, 2845
SinI GGWCC 5 cut(s) 166, 353, 1001, 1361, 1940
SmiMI CAYNNNNRTG 3 cut(s) 2715, 2930, 3056
SmlI CTYRAG 3 cut(s) 452, 677, 740
SmoI CTYRAG 3 cut(s) 452, 677, 740
SsiI CCGC 5 cut(s) 57, 1071, 1507, 1805, 2018
SspI AATATT 5 cut(s) 418, 1025, 1222, 2464, 2683
SspMI CTAG 9 cut(s) 150, 672, 1613, 1878, 1907, 2270, 2690, 2903, 2996
StyD4I CCNGG 5 cut(s) 630, 1194, 2173, 2493, 2927
StyI CCWWGG 3 cut(s) 442, 651, 794
TaaI ACNGT 9 cut(s) 236, 322, 903, 1053, 1110, 2104, 2417, 2663, 3074
TaiI ACGT 3 cut(s) 470, 1433, 1487
TaqI TCGA 7 cut(s) 1056, 1168, 1561, 2976, 3080, 3085, 3113
TatI WGTACW 3 cut(s) 759, 2232, 2520
TscAI CASTG 7 cut(s) 102, 283, 355, 393, 1363, 1507, 1798
TseFI GTSAC 3 cut(s) 1471, 2558, 2923
TseI GCWGC 4 cut(s) 1983, 2111, 2114, 2609
Tsp45I GTSAC 3 cut(s) 1471, 2558, 2923
TspGWI ACGGA 3 cut(s) 2240, 3129, 3191
TspRI CASTG 7 cut(s) 102, 283, 355, 393, 1363, 1507, 1798
Tth111I GACNNNGTC 1 cut(s) 2921
Van91I CCANNNNNTGG 2 cut(s) 731, 1814
VpaK11BI GGWCC 5 cut(s) 166, 353, 1001, 1361, 1940
VspI ATTAAT 1 cut(s) 987
XapI RAATTY 6 cut(s) 252, 556, 800, 1352, 1835, 1872
XbaI TCTAGA 1 cut(s) 2995
XceI RCATGY 2 cut(s) 1282, 3022
XmiI GTMKAC 1 cut(s) 405
XmnI GAANNNNTTC 3 cut(s) 811, 1352, 2550
XspI CTAG 9 cut(s) 150, 672, 1613, 1878, 1907, 2270, 2690, 2903, 2996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.