pycom14g09870

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012922
Physical Location & Seq
Reverse (-)
12129990 .. 12134418
4429 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1989 bp
ATGAGAAGTTTTGCCTCCTTCCGGTTTCTGTGCTGCTTGATTTCTGGGGTTCTTCTTGTGGGTGGTGAATCTTTTGCTTCAAAAGAAGTTTTGGCCCTCACAACCTTTAAGGAAGCAATATATGAAGACCCGCATTTGGTTTTGTCCACTTGGAATGCCTTGGATGCCGATCGATCTTGTGCCTGGTCCGGCATCACATGTTCTGTGGCTCGGGACCATGTATTCAAGATTAATATTTCCAGTTCATCAGTAAGGGGATTTCTTGTACCAGACTTGGGTCAACTCACCTTCTTGCAAGAACTAAATTTGCATGGGAACAAGCTACTTGGAACAATACCTAAAGAGCTGGGCTTGTTAAAATACCTCACAATCTTGGACTTGGGAGTGAATGAACTCACCGGTCCAATTCCTCCAGAGCTTGGGAATTTAACCAGTATTGTGAAAATAAATCTTCAGTCAAATGGGTTGTCTGGTCGGCTACCTCCTGAACTTGGCAATTTGGGGCACCTTGAGGAACTTCGTTTGGATAGGAATAAGCTTCAAGGAACCATTCCTTCCAATGGCTATACAAGATTCCCATCTAACGTACCTGGAATGTATGCCTCACAATCAAACCTAACAGGCCTTTGTCGATCGTCTCAGTTAAAAGTTGCAGATTTCTCGTACAACTTCTTTGTTGGTAGCATACCTAAGTGCTTGGAGTATCTTCCCAGGACAAGTTTTCAAGGAAATTGTCTCCAAAAACAGGATCCCAGGCAGCGTCCTGTAGAACATTGTGCTGGTGCACCCCCTTCGAAAGGCCATCCCGGAAGTAACCCAAAGCATACACCTGCTAAAGTGGTCTCCAAACATCGGGAGGCTTCAAAACCTGCCTGGCTTTTGGCTCTGGAAATAGTCACCGGAACCATGGTGTGTTCGCTCTTTCTAGTTGCTGTTTTCACTGCTGTTCACAAATGCAATAGCAAATCATCTATCATAATTCCTTGGAAGAAATCAGCAAGTGAAAAAGACCATATCACAGTCTACATAGATTCCGAGATGTTAAAAGATGTTGCCAGATTCAGTAGACAAGAGCTAGAGGTTGCTTGTGAAGACTTTAGCAACATTATCGGTTCCTCACCTGACAGTTTGGTCTACAAAGGCAACATAAAAGGTGGTCCAGAGATTGCTGTGATATCCCTTTGCATTAAGGAAGAGCATTGGACGGGCTATCTTGAACTCTATTTTCAGAGAGAGGTGACAGATTTGGCAAGATTAAATCACGAAAATGCAGCAAAATTACTGGGTTATTGTAGTGAGAATGCCCCCTTTACAAGGATGCTGGTTTTTGAATATGCTTCAAATGGGACACTGTATGAACACCTACATTTTGGAGAAGGATGCCAGCTAAGTTGGACAAGGCGTATGAAAATTATTATAGGCATTGCTCAGGGACTCAAGTATTTTCACTCGGAACTTGAGCCGCCATTTACCATATCAGAGTTAAATTCTAGTGCTGTATATCTTACAGAAGATTTTTTACCCAAGCTCGTTGATTTTGAAAGTTGGAAGACAATTCTTGCGAGATCGGAAAAGAACTCAGGCACTATTTGCGGCGAAGGTGCTATTTGTGTCCTTCCGAATTCTATGGAAGCACGCCACCTAGATGTTAAAGGCAATGTTTATGCTTTCGGCATACTTTTACTCGAAGTAATCAGCGGGAGGCCTCCATACTGCAAGGATAAGGGGTGCTTGATAGACTGGGCGAAAGACTATCTTGAGCTGCCAGATGTGATGTCGTACGTTGTGGATCCCGAACTGAAACATTTCAGTTATGATGACCTCAAAGTTATATGTGATGTGGTGAATCTTTGCATCCATCCCGACCCAACCAAGCGGCCATCGATGCAGGAACTATGCACCATTTTGGAGAGCAAAATCAACACATCAGTCTCTGCTGAGCTCAAGGCGTCTAATTTGGCGTGGGCGGAGCTTGCGCTTTCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

663

Amino Acids

73.32

Weight (kDa)

6.25

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 68 8.3e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 89 - 176 2.4e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 369 - 634 2.7e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 372 - 633 1.6e-15 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012922)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63430 AT1G63430 AT1G63430
fragaria_vesca FvH4_5g02060
malus_domestica MD06G1079300.v1.1 MD14G1101900.v1.1
prunus_persica Prupe.5G097500_v2.0.a1
pyrus_communis pycom06g07640 pycom14g09870
rosa_chinensis RchiOBHm_Chr7g0198241
rosa_laevigata RLG00000003881
rosa_multiflora Rmu_sc0001250.1_g000046
rosa_roxburghii Rroxscaffold_3G00257900
rosa_rugosa Rorug07G0046100
rosa_samantha Rh7BG172900 Rh7CG179400 Rh7DG172200
rosa_wichuraiana Rw7G014840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 838
AasI GACNNNNNNGTC 1 cut(s) 1130
Acc36I ACCTGC 2 cut(s) 838, 877
AccB1I GGYRCC 1 cut(s) 504
AccB7I CCANNNNNTGG 1 cut(s) 419
AccI GTMKAC 3 cut(s) 1023, 1066, 1134
AciI CCGC 6 cut(s) 131, 1463, 1593, 1698, 1876, 1967
AclWI GGATC 4 cut(s) 743, 756, 1784, 1797
AcoI YGGCCR 1 cut(s) 1877
AcsI RAATTY 4 cut(s) 304, 424, 1486, 1621
AcuI CTGAAG 1 cut(s) 437
AcyI GRCGYC 1 cut(s) 1949
AfaI GTAC 4 cut(s) 267, 588, 665, 1781
AflIII ACRYGT 1 cut(s) 197
AgeI ACCGGT 1 cut(s) 398
AgsI TTSAA 9 cut(s) 81, 226, 542, 725, 864, 1217, 1331, 1341, 1541
AjnI CCWGG 5 cut(s) 182, 589, 710, 752, 872
Alw21I GWGCWC 2 cut(s) 787, 1944
Alw26I GTCTC 4 cut(s) 642, 740, 847, 1936
Alw44I GTGCAC 1 cut(s) 783
AlwI GGATC 4 cut(s) 743, 756, 1784, 1797
AlwNI CAGNNNCTG 1 cut(s) 1934
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 5 cut(s) 93, 622, 799, 1703, 1877
ApaLI GTGCAC 1 cut(s) 783
ApeKI GCWGC 4 cut(s) 33, 757, 1271, 1762
ApoI RAATTY 4 cut(s) 304, 424, 1486, 1621
ArsI GACNNNNNNTTYG 2 cut(s) 452, 484
AseI ATTAAT 1 cut(s) 231
AsiGI ACCGGT 1 cut(s) 398
Asp700I GAANNNNTTC 1 cut(s) 1805
AspLEI GCGC 1 cut(s) 1978
AspS9I GGNCC 5 cut(s) 94, 186, 214, 401, 1157
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 7 cut(s) 77, 277, 388, 889, 1110, 1249, 1855
AsuII TTCGAA 1 cut(s) 794
AvaI CYCGRG 1 cut(s) 210
AvaII GGWCC 4 cut(s) 186, 214, 401, 1157
BaeGI GKGCMC 2 cut(s) 507, 787
BamHI GGATCC 2 cut(s) 748, 1789
BanI GGYRCC 1 cut(s) 504
BanII GRGCYC 1 cut(s) 1944
BarI GAAGNNNNNNTAC 2 cut(s) 1503, 1535
BbsI GAAGAC 3 cut(s) 132, 1098, 1556
Bbv12I GWGCWC 2 cut(s) 787, 1944
BbvI GCAGC 4 cut(s) 20, 769, 1283, 1749
BccI CCATC 4 cut(s) 586, 810, 1866, 1888
BcgI CGANNNNNNTGC 6 cut(s) 642, 676, 774, 808, 1090, 1124
BciT130I CCWGG 5 cut(s) 184, 591, 712, 754, 874
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 4 cut(s) 642, 740, 847, 1936
BfaI CTAG 4 cut(s) 926, 1076, 1491, 1643
BfmI CTRYAG 1 cut(s) 765
BfuAI ACCTGC 2 cut(s) 838, 877
BisI GCNGC 7 cut(s) 34, 758, 1272, 1463, 1594, 1763, 1877
BlpI GCTNAGC 1 cut(s) 1938
BlsI GCNGC 7 cut(s) 35, 759, 1273, 1464, 1595, 1764, 1878
Bme1390I CCNGG 6 cut(s) 184, 591, 712, 754, 807, 874
Bme18I GGWCC 4 cut(s) 186, 214, 401, 1157
BmeT110I CYCGRG 1 cut(s) 210
BmgT120I GGNCC 5 cut(s) 94, 186, 214, 401, 1157
BmiI GGNNCC 7 cut(s) 215, 506, 547, 750, 904, 1114, 1791
BmrFI CCNGG 6 cut(s) 184, 591, 712, 754, 807, 874
BmrI ACTGGG 2 cut(s) 1292, 1750
BmsI GCATC 6 cut(s) 154, 201, 1308, 1370, 1863, 1875
BmuI ACTGGG 2 cut(s) 1292, 1750
BoxI GACNNNNGTC 1 cut(s) 276
BpiI GAAGAC 3 cut(s) 132, 1098, 1556
BpmI CTGGAG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 1428
Bpu1102I GCTNAGC 1 cut(s) 1938
Bpu14I TTCGAA 1 cut(s) 794
BpuEI CTTGAG 5 cut(s) 530, 1421, 1478, 1778, 1928
BpuMI CCSGG 1 cut(s) 807
Bsa29I ATCGAT 2 cut(s) 172, 1883
BsaBI GATNNNNATC 2 cut(s) 168, 577
BsaHI GRCGYC 1 cut(s) 1949
BsaI GGTCTC 1 cut(s) 847
BsaJI CCNNGG 5 cut(s) 159, 710, 752, 906, 983
BsaWI WCCGGW 3 cut(s) 21, 398, 899
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 4 cut(s) 240, 432, 1287, 1745
Bse3DI GCAATG 2 cut(s) 1422, 1663
Bse8I GATNNNNATC 2 cut(s) 168, 577
BseBI CCWGG 5 cut(s) 184, 591, 712, 754, 874
BseCI ATCGAT 2 cut(s) 172, 1883
BseDI CCNNGG 5 cut(s) 159, 710, 752, 906, 983
BseGI GGATG 6 cut(s) 169, 802, 1323, 1385, 1854, 1858
BseJI GATNNNNATC 2 cut(s) 168, 577
BseMI GCAATG 2 cut(s) 1422, 1663
BseMII CTCAG 4 cut(s) 653, 1442, 1593, 1929
BseNI ACTGG 4 cut(s) 240, 432, 1287, 1745
BseSI GKGCMC 2 cut(s) 507, 787
BseXI GCAGC 4 cut(s) 20, 769, 1283, 1749
BseYI CCCAGC 1 cut(s) 346
Bsh1285I CGRYCG 2 cut(s) 172, 635
BshFI GGCC 5 cut(s) 95, 624, 801, 1705, 1879
BshNI GGYRCC 1 cut(s) 504
BshTI ACCGGT 1 cut(s) 398
BshVI ATCGAT 2 cut(s) 172, 1883
BsiEI CGRYCG 2 cut(s) 172, 635
BsiHKAI GWGCWC 2 cut(s) 787, 1944
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 5 cut(s) 22, 189, 399, 807, 900
BsiWI CGTACG 1 cut(s) 1779
BslFI GGGAC 3 cut(s) 227, 1360, 1446
BsmAI GTCTC 4 cut(s) 642, 740, 847, 1936
BsmBI CGTCTC 1 cut(s) 642
BsmFI GGGAC 3 cut(s) 227, 1360, 1446
BsmI GAATGC 2 cut(s) 160, 1306
BsnI GGCC 5 cut(s) 95, 624, 801, 1705, 1879
Bso31I GGTCTC 1 cut(s) 847
BsoBI CYCGRG 1 cut(s) 210
Bsp119I TTCGAA 1 cut(s) 794
Bsp1286I GDGCHC 3 cut(s) 507, 787, 1944
Bsp143I GATC 6 cut(s) 169, 173, 632, 748, 1565, 1789
Bsp1720I GCTNAGC 1 cut(s) 1938
Bsp19I CCATGG 1 cut(s) 906
BspACI CCGC 6 cut(s) 131, 1463, 1593, 1698, 1876, 1967
BspANI GGCC 5 cut(s) 95, 624, 801, 1705, 1879
BspCNI CTCAG 4 cut(s) 652, 1441, 1592, 1930
BspDI ATCGAT 2 cut(s) 172, 1883
BspLI GGNNCC 7 cut(s) 215, 506, 547, 750, 904, 1114, 1791
BspMI ACCTGC 2 cut(s) 838, 877
BspPI GGATC 4 cut(s) 743, 756, 1784, 1797
BspQI GCTCTTC 1 cut(s) 1188
BspT104I TTCGAA 1 cut(s) 794
BspT107I GGYRCC 1 cut(s) 504
BspTNI GGTCTC 1 cut(s) 847
BsrDI GCAATG 2 cut(s) 1422, 1663
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 4 cut(s) 240, 432, 1287, 1745
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 5 cut(s) 159, 710, 752, 906, 983
BssMI GATC 6 cut(s) 169, 173, 632, 748, 1565, 1789
BssNI GRCGYC 1 cut(s) 1949
BssT1I CCWWGG 3 cut(s) 159, 906, 983
Bst2UI CCWGG 5 cut(s) 184, 591, 712, 754, 874
Bst4CI ACNGT 3 cut(s) 1021, 1127, 1353
Bst6I CTCTTC 1 cut(s) 1188
BstACI GRCGYC 1 cut(s) 1949
BstAPI GCANNNNNTGC 1 cut(s) 1590
BstBI TTCGAA 1 cut(s) 794
BstC8I GCNNGC 3 cut(s) 1385, 1636, 1974
BstDEI CTNAG 6 cut(s) 639, 690, 1388, 1428, 1579, 1938
BstDSI CCRYGG 1 cut(s) 906
BstENI CCTNNNNNAGG 2 cut(s) 795, 1312
BstF5I GGATG 6 cut(s) 169, 802, 1323, 1385, 1854, 1858
BstHHI GCGC 1 cut(s) 1978
BstKTI GATC 6 cut(s) 172, 176, 635, 751, 1568, 1792
BstMAI GTCTC 4 cut(s) 642, 740, 847, 1936
BstMBI GATC 6 cut(s) 169, 173, 632, 748, 1565, 1789
BstMCI CGRYCG 2 cut(s) 172, 635
BstMWI GCNNNNNNNGC 4 cut(s) 164, 1590, 1885, 1973
BstNI CCWGG 5 cut(s) 184, 591, 712, 754, 874
BstNSI RCATGY 1 cut(s) 201
BstPAI GACNNNNGTC 1 cut(s) 276
BstSCI CCNGG 6 cut(s) 182, 589, 710, 752, 805, 872
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 2 cut(s) 507, 787
BstV1I GCAGC 4 cut(s) 20, 769, 1283, 1749
BstV2I GAAGAC 3 cut(s) 132, 1098, 1556
BstX2I RGATCY 2 cut(s) 748, 1789
BstYI RGATCY 2 cut(s) 748, 1789
Bsu15I ATCGAT 2 cut(s) 172, 1883
BsuRI GGCC 5 cut(s) 95, 624, 801, 1705, 1879
BsuTUI ATCGAT 2 cut(s) 172, 1883
BtgI CCRYGG 1 cut(s) 906
BtsCI GGATG 6 cut(s) 169, 802, 1323, 1385, 1854, 1858
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 2 cut(s) 939, 1349
BveI ACCTGC 2 cut(s) 838, 877
Cac8I GCNNGC 3 cut(s) 1385, 1636, 1974
CaiI CAGNNNCTG 1 cut(s) 1934
CfoI GCGC 1 cut(s) 1978
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 5 cut(s) 94, 186, 214, 401, 1157
ClaI ATCGAT 2 cut(s) 172, 1883
CseI GACGC 2 cut(s) 749, 1938
Csp6I GTAC 4 cut(s) 266, 587, 664, 1780
CspAI ACCGGT 1 cut(s) 398
CviAII CATG 4 cut(s) 198, 218, 311, 907
CviQI GTAC 4 cut(s) 266, 587, 664, 1780
DdeI CTNAG 6 cut(s) 639, 690, 1388, 1428, 1579, 1938
DpnI GATC 6 cut(s) 171, 175, 634, 750, 1567, 1791
DpnII GATC 6 cut(s) 169, 173, 632, 748, 1565, 1789
DrdI GACNNNNNNGTC 1 cut(s) 1130
DseDI GACNNNNNNGTC 1 cut(s) 1130
EaeI YGGCCR 1 cut(s) 1877
Eam1104I CTCTTC 1 cut(s) 1188
EarI CTCTTC 1 cut(s) 1188
EciI GGCGGA 1 cut(s) 1982
Ecl136II GAGCTC 1 cut(s) 1942
Eco130I CCWWGG 3 cut(s) 159, 906, 983
Eco147I AGGCCT 2 cut(s) 624, 1705
Eco24I GRGCYC 1 cut(s) 1944
Eco31I GGTCTC 1 cut(s) 847
Eco32I GATATC 1 cut(s) 1176
Eco47I GGWCC 4 cut(s) 186, 214, 401, 1157
Eco53kI GAGCTC 1 cut(s) 1942
Eco57I CTGAAG 1 cut(s) 437
Eco88I CYCGRG 1 cut(s) 210
EcoICRI GAGCTC 1 cut(s) 1942
EcoNI CCTNNNNNAGG 2 cut(s) 795, 1312
EcoRI GAATTC 1 cut(s) 1621
EcoRII CCWGG 5 cut(s) 182, 589, 710, 752, 872
EcoRV GATATC 1 cut(s) 1176
EcoT14I CCWWGG 3 cut(s) 159, 906, 983
EcoT38I GRGCYC 1 cut(s) 1944
ErhI CCWWGG 3 cut(s) 159, 906, 983
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 4 cut(s) 201, 221, 314, 910
FalI AAGNNNNNCTT 4 cut(s) 1715, 1747, 1740, 1772
FaqI GGGAC 3 cut(s) 227, 1360, 1446
FatI CATG 4 cut(s) 197, 217, 310, 906
FauI CCCGC 2 cut(s) 138, 1691
FblI GTMKAC 3 cut(s) 1023, 1066, 1134
Fnu4HI GCNGC 7 cut(s) 34, 758, 1272, 1463, 1594, 1763, 1877
FokI GGATG 6 cut(s) 176, 789, 1330, 1392, 1841, 1845
FriOI GRGCYC 1 cut(s) 1944
Fsp4HI GCNGC 7 cut(s) 34, 758, 1272, 1463, 1594, 1763, 1877
FspBI CTAG 4 cut(s) 926, 1076, 1491, 1643
GlaI GCGC 1 cut(s) 1977
GluI GCNGC 7 cut(s) 34, 758, 1272, 1463, 1594, 1763, 1877
GsaI CCCAGC 1 cut(s) 350
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 5 cut(s) 95, 624, 801, 1705, 1879
HapII CCGG 5 cut(s) 22, 189, 399, 807, 900
HgaI GACGC 2 cut(s) 749, 1938
HhaI GCGC 1 cut(s) 1978
Hin1I GRCGYC 1 cut(s) 1949
Hin1II CATG 4 cut(s) 201, 221, 314, 910
Hin6I GCGC 1 cut(s) 1976
HinP1I GCGC 1 cut(s) 1976
HincII GTYRAC 1 cut(s) 281
HindII GTYRAC 1 cut(s) 281
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 6 cut(s) 68, 573, 1031, 1059, 1434, 1846
HpaII CCGG 5 cut(s) 22, 189, 399, 807, 900
HphI GGTGA 7 cut(s) 77, 277, 388, 889, 1110, 1249, 1855
Hpy166II GTNNAC 7 cut(s) 147, 281, 785, 949, 1024, 1067, 1135
Hpy188I TCNGA 6 cut(s) 1036, 1230, 1453, 1480, 1570, 1620
Hpy8I GTNNAC 7 cut(s) 147, 281, 785, 949, 1024, 1067, 1135
HpyAV CCTTC 7 cut(s) 28, 298, 564, 801, 1370, 1592, 1625
HpyCH4III ACNGT 3 cut(s) 1021, 1127, 1353
HpyCH4IV ACGT 2 cut(s) 585, 1782
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 1590, 1885, 1973
HpyF3I CTNAG 6 cut(s) 639, 690, 1388, 1428, 1579, 1938
HpySE526I ACGT 2 cut(s) 585, 1782
Hsp92I GRCGYC 1 cut(s) 1949
Hsp92II CATG 4 cut(s) 201, 221, 314, 910
HspAI GCGC 1 cut(s) 1976
Kzo9I GATC 6 cut(s) 169, 173, 632, 748, 1565, 1789
LguI GCTCTTC 1 cut(s) 1188
LmnI GCTCC 1 cut(s) 1969
Lsp1109I GCAGC 4 cut(s) 20, 769, 1283, 1749
LweI GCATC 6 cut(s) 154, 201, 1308, 1370, 1863, 1875
MaeI CTAG 4 cut(s) 926, 1076, 1491, 1643
MaeII ACGT 2 cut(s) 585, 1782
MaeIII GTNAC 3 cut(s) 812, 895, 1237
MalI GATC 6 cut(s) 171, 175, 634, 750, 1567, 1791
MboI GATC 6 cut(s) 169, 173, 632, 748, 1565, 1789
MboII GAAGA 9 cut(s) 44, 137, 443, 698, 1000, 1103, 1205, 1523, 1561
MflI RGATCY 2 cut(s) 748, 1789
MhlI GDGCHC 3 cut(s) 507, 787, 1944
MlyI GAGTC 1 cut(s) 1428
MmeI TCCRAC 2 cut(s) 1373, 1526
MroXI GAANNNNTTC 1 cut(s) 1805
MslI CAYNNNNRTG 2 cut(s) 1266, 1644
MspA1I CMGCKG 1 cut(s) 1698
MspI CCGG 5 cut(s) 22, 189, 399, 807, 900
MspR9I CCNGG 6 cut(s) 184, 591, 712, 754, 807, 874
Mva1269I GAATGC 2 cut(s) 160, 1306
MvaI CCWGG 5 cut(s) 184, 591, 712, 754, 874
MwoI GCNNNNNNNGC 4 cut(s) 164, 1590, 1885, 1973
NciI CCSGG 1 cut(s) 807
NcoI CCATGG 1 cut(s) 906
NdeII GATC 6 cut(s) 169, 173, 632, 748, 1565, 1789
NlaIII CATG 4 cut(s) 201, 221, 314, 910
NlaIV GGNNCC 7 cut(s) 215, 506, 547, 750, 904, 1114, 1791
NmuCI GTSAC 2 cut(s) 895, 1237
NspI RCATGY 1 cut(s) 201
NspV TTCGAA 1 cut(s) 794
PaqCI CACCTGC 1 cut(s) 838
PceI AGGCCT 2 cut(s) 624, 1705
PciI ACATGT 1 cut(s) 197
PciSI GCTCTTC 1 cut(s) 1188
PctI GAATGC 2 cut(s) 160, 1306
PdmI GAANNNNTTC 1 cut(s) 1805
PfeI GAWTC 5 cut(s) 68, 573, 1031, 1059, 1846
Pfl23II CGTACG 1 cut(s) 1779
PflMI CCANNNNNTGG 1 cut(s) 419
PfoI TCCNGGA 1 cut(s) 805
PinAI ACCGGT 1 cut(s) 398
PkrI GCNGC 7 cut(s) 35, 759, 1273, 1464, 1595, 1764, 1878
Ple19I CGATCG 2 cut(s) 172, 635
PleI GAGTC 1 cut(s) 1428
PpsI GAGTC 1 cut(s) 1428
PscI ACATGT 1 cut(s) 197
PshAI GACNNNNGTC 1 cut(s) 276
PshBI ATTAAT 1 cut(s) 231
Psp124BI GAGCTC 1 cut(s) 1944
Psp6I CCWGG 5 cut(s) 182, 589, 710, 752, 872
PspFI CCCAGC 1 cut(s) 346
PspGI CCWGG 5 cut(s) 182, 589, 710, 752, 872
PspLI CGTACG 1 cut(s) 1779
PspN4I GGNNCC 7 cut(s) 215, 506, 547, 750, 904, 1114, 1791
PspPI GGNCC 5 cut(s) 94, 186, 214, 401, 1157
PstNI CAGNNNCTG 1 cut(s) 1934
PsuI RGATCY 2 cut(s) 748, 1789
PvuI CGATCG 2 cut(s) 172, 635
RsaI GTAC 4 cut(s) 267, 588, 665, 1781
RsaNI GTAC 4 cut(s) 266, 587, 664, 1780
RseI CAYNNNNRTG 2 cut(s) 1266, 1644
SacI GAGCTC 1 cut(s) 1944
SapI GCTCTTC 1 cut(s) 1188
SatI GCNGC 7 cut(s) 34, 758, 1272, 1463, 1594, 1763, 1877
Sau3AI GATC 6 cut(s) 169, 173, 632, 748, 1565, 1789
Sau96I GGNCC 5 cut(s) 94, 186, 214, 401, 1157
SchI GAGTC 1 cut(s) 1428
ScrFI CCNGG 6 cut(s) 184, 591, 712, 754, 807, 874
SduI GDGCHC 3 cut(s) 507, 787, 1944
SfaNI GCATC 6 cut(s) 154, 201, 1308, 1370, 1863, 1875
SfcI CTRYAG 1 cut(s) 765
SfuI TTCGAA 1 cut(s) 794
SinI GGWCC 4 cut(s) 186, 214, 401, 1157
SmiMI CAYNNNNRTG 2 cut(s) 1266, 1644
SmlI CTYRAG 5 cut(s) 509, 1436, 1457, 1757, 1943
SmoI CTYRAG 5 cut(s) 509, 1436, 1457, 1757, 1943
SseBI AGGCCT 2 cut(s) 624, 1705
SsiI CCGC 6 cut(s) 131, 1463, 1593, 1698, 1876, 1967
SspI AATATT 1 cut(s) 235
SspMI CTAG 4 cut(s) 926, 1076, 1491, 1643
SstI GAGCTC 1 cut(s) 1944
StuI AGGCCT 2 cut(s) 624, 1705
StyD4I CCNGG 6 cut(s) 182, 589, 710, 752, 805, 872
StyI CCWWGG 3 cut(s) 159, 906, 983
TaaI ACNGT 3 cut(s) 1021, 1127, 1353
TaiI ACGT 2 cut(s) 588, 1785
TaqI TCGA 5 cut(s) 172, 631, 794, 1686, 1883
TauI GCSGC 3 cut(s) 1465, 1596, 1879
TfiI GAWTC 5 cut(s) 68, 573, 1031, 1059, 1846
TscAI CASTG 2 cut(s) 946, 1356
TseFI GTSAC 2 cut(s) 895, 1237
TseI GCWGC 4 cut(s) 33, 757, 1271, 1762
Tsp45I GTSAC 2 cut(s) 895, 1237
TspDTI ATGAA 6 cut(s) 138, 234, 405, 1371, 1421, 1971
TspRI CASTG 2 cut(s) 946, 1356
Van91I CCANNNNNTGG 1 cut(s) 419
VneI GTGCAC 1 cut(s) 783
VpaK11BI GGWCC 4 cut(s) 186, 214, 401, 1157
VspI ATTAAT 1 cut(s) 231
XagI CCTNNNNNAGG 2 cut(s) 795, 1312
XapI RAATTY 4 cut(s) 304, 424, 1486, 1621
XceI RCATGY 1 cut(s) 201
XmiI GTMKAC 3 cut(s) 1023, 1066, 1134
XmnI GAANNNNTTC 1 cut(s) 1805
XspI CTAG 4 cut(s) 926, 1076, 1491, 1643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.