Rroxscaffold_3G00257900

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012922
Physical Location & Seq
Reverse (-)
52198085 .. 52202933
4849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257900.1

Sequence Viewer

Length: 1794 bp
ATGCGCTATGACTCGAGACCATGTTATAAAGATCAACATTTCCGGTTCATCTATAAGGGGTTTCTTGTACCAGACTTGGGTGATCTCACCTTCTTGCAAGAACTAATTTTGCATGGGAACAGGCTGCTTGGAATATTACCTAAGGAACTGGGTTCGTTAAAATACCTGACTATCTTGGATTTGGGATTGAATGAACTCACTGGTCCAATTCCTCCGGAGCTTGGGAACTTGACCAACGTTGTGAAAATAAATCTCCAGTCGAATGGGTTGTCTGGTAAGCTACCTCCTGAACTTGGCAATTTGGGATACCTTAAGGAACTTTATTTGGACAGGAATAAGCTTCAAGGAACTGTTCCTGCTGGTGGCAATAAAGGAATCACATCTAGTGATTCTAACGTACGTGGGATGTATGCCTCAAACTTGACAGGCCTTTGCCGCTCATCTCAGTTAAGAGTAGCTGATCTCTCATACAACTTCTTTGTTGGTAGCATACCTAAGTGCTTGGAGTATCTTCCGAGGACTAGCTTTCAGGGAAATTGCCTCCAGAAACAAGATCCGAAACAGCGTCCTGCAGATATGTGTGTTGGTTCTCCACCTTCTAAAGGCCATCCTGCAGTTAACCCAAAGCACAAACCTACTAAAGATGTCCCCAAACATCCAGACACGTCAAAGCCAGCCTGGCTTTTGGCCTTGGAAGTAGTGACAGGAACCATGGTGTGCTCTCTTGTTTTAGTTGCTGTTGTCACTGGTCTTCAGAAATGCAATAGCAAATCTTCTATAATAATTCCTTGGAAGAAATCAGGAACTGAAAAGGACCATACTACAATCTACATAGATTCCGAGATGTTGAAGGATGTTGTTAGATTCAACAGACAAGATCTTGAAGTAGCGTGTGAAGACTTCAGCAATATTATTGGCTCCTCACCTGATAGTTTGGTTTACAAGGGCAACATGAAAGGTGGGCCAGAGATCGCTGTGATATCCCTTTGCATTAAGGAAGAGCATTGGACAGGCTATCTTGAGCTCTATTTTCAGAGAGAGGTGACAGATTTGTCAAGATTGACTCACGAGAATACAGGAAAGTTACTGGGTTATTGTAGTGAGAGCACCCCATTTACAAGGATGCTGGTTTTTGAATATGCTTCAAATGGGACACTGTATGAGCACCTTCATTATGGAGAAGGATGCCAGTTAAGTTGGACAAGGCGCATGAAAATTGTAATAGGCATTGCTCGGGGGCTCAAGTATCTCCACATGGAACTTGAGCCACCATTTACAATATCAGAGTTAAATTCTAGTGCCGTATATCTTACAGATGATTTCTTGCCCAAGCTGGTTGATTTTGAAAGTTGGAAGACAATTATTGCGCGGTCAGAAAAAAACTCTGGTTCTATTGGCAGCCAAGGTGCTATTTGTGTTCTTCCAAATTCTATGGAAGCACGCCACCTGGATGTTCAAGGCAATGTTTGTGCTTTTGGGGTACTTTTACTGGAAATAATTAGTGGGAGGCCTCCATACTGCAAGGATAAAGGGTGCTTGGTAGACTGGGCCAAAGATTATCTTGAGCTGCCAGACGTGATGCCTCATGTGGTGGATCCTGAATTGAAACATTTCAGTTGTGATGACCTCAAGGTTCTATGTGATGTGGTAAATCTCTGTATCCATCCAGAACCCACCAAGCGGCCATCGATGCAGGAAGTATGCACCATGTTAGAGAGCAAAATCGACACATCGGTGTCGCTTGAGCTTAAAGCATCTTCTTTGGCATGGGCTGAGCTTGCACTTTCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

597

Amino Acids

66.34

Weight (kDa)

6.33

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 303 - 570 7.8e-18 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 305 - 569 2.5e-12 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012922)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63430 AT1G63430 AT1G63430
fragaria_vesca FvH4_5g02060
malus_domestica MD06G1079300.v1.1 MD14G1101900.v1.1
prunus_persica Prupe.5G097500_v2.0.a1
pyrus_communis pycom06g07640 pycom14g09870
rosa_chinensis RchiOBHm_Chr7g0198241
rosa_laevigata RLG00000003881
rosa_multiflora Rmu_sc0001250.1_g000046
rosa_roxburghii Rroxscaffold_3G00257900
rosa_rugosa Rorug07G0046100
rosa_samantha Rh7BG172900 Rh7CG179400 Rh7DG172200
rosa_wichuraiana Rw7G014840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 27
AasI GACNNNNNNGTC 1 cut(s) 1051
AccBSI CCGCTC 1 cut(s) 438
AccI GTMKAC 1 cut(s) 1542
AccII CGCG 1 cut(s) 1369
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 3 cut(s) 436, 1369, 1681
AclI AACGTT 1 cut(s) 237
AclWI GGATC 3 cut(s) 548, 1589, 1602
AcoI YGGCCR 1 cut(s) 1682
AcsI RAATTY 2 cut(s) 1291, 1426
AcuI CTGAAG 2 cut(s) 737, 886
AfaI GTAC 3 cut(s) 69, 399, 1482
AfiI CCNNNNNNNGG 6 cut(s) 77, 221, 293, 362, 602, 1680
AflII CTTAAG 1 cut(s) 311
AflIII ACRYGT 1 cut(s) 663
AjiI CACGTC 2 cut(s) 666, 1576
AjnI CCWGG 2 cut(s) 677, 1446
AleI CACNNNNGTG 1 cut(s) 1733
Alw21I GWGCWC 4 cut(s) 722, 1026, 1109, 1167
Alw26I GTCTC 1 cut(s) 10
AlwI GGATC 3 cut(s) 548, 1589, 1602
AlwNI CAGNNNCTG 1 cut(s) 806
Ama87I CYCGRG 2 cut(s) 13, 1233
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 7 cut(s) 427, 604, 687, 962, 1508, 1548, 1682
ApeKI GCWGC 3 cut(s) 124, 1398, 1567
ApoI RAATTY 2 cut(s) 1291, 1426
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
Asp700I GAANNNNTTC 1 cut(s) 1610
AspLEI GCGC 3 cut(s) 6, 1209, 1369
AspS9I GGNCC 4 cut(s) 203, 814, 962, 1548
AsuHPI GGTGA 4 cut(s) 79, 92, 915, 1054
AvaI CYCGRG 2 cut(s) 13, 1233
AvaII GGWCC 2 cut(s) 203, 814
AxyI CCTNAGG 1 cut(s) 141
BamHI GGATCC 1 cut(s) 1594
BanII GRGCYC 2 cut(s) 1026, 1242
BauI CACGAG 1 cut(s) 1067
BbsI GAAGAC 3 cut(s) 743, 903, 1361
Bbv12I GWGCWC 4 cut(s) 722, 1026, 1109, 1167
BbvI GCAGC 3 cut(s) 111, 1410, 1554
BccI CCATC 3 cut(s) 615, 1671, 1693
BceAI ACGGC 1 cut(s) 1286
BciT130I CCWGG 2 cut(s) 679, 1448
BciVI GTATCC 2 cut(s) 299, 1670
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 3 cut(s) 384, 522, 1296
BfmI CTRYAG 2 cut(s) 570, 612
BfrI CTTAAG 1 cut(s) 311
BfuI GTATCC 2 cut(s) 299, 1670
BglI GCCNNNNNGGC 1 cut(s) 679
BglII AGATCT 1 cut(s) 877
BisI GCNGC 5 cut(s) 125, 436, 1399, 1568, 1682
BlpI GCTNAGC 1 cut(s) 1773
BlsI GCNGC 5 cut(s) 126, 437, 1400, 1569, 1683
Bme1390I CCNGG 2 cut(s) 679, 1448
Bme18I GGWCC 2 cut(s) 203, 814
BmeT110I CYCGRG 2 cut(s) 13, 1233
BmgBI CACGTC 2 cut(s) 666, 1576
BmgT120I GGNCC 4 cut(s) 203, 814, 962, 1548
BmiI GGNNCC 3 cut(s) 709, 919, 1596
BmrFI CCNGG 2 cut(s) 679, 1448
BmrI ACTGGG 3 cut(s) 158, 1097, 1555
BmsI GCATC 5 cut(s) 1113, 1175, 1569, 1680, 1763
BmuI ACTGGG 3 cut(s) 158, 1097, 1555
BpiI GAAGAC 3 cut(s) 743, 903, 1361
BpmI CTGGAG 2 cut(s) 239, 527
Bpu1102I GCTNAGC 1 cut(s) 1773
BpuEI CTTGAG 6 cut(s) 1040, 1226, 1283, 1583, 1613, 1763
Bsa29I ATCGAT 1 cut(s) 1688
BsaAI YACGTR 1 cut(s) 401
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 5 cut(s) 515, 690, 711, 788, 1402
BsaWI WCCGGW 2 cut(s) 42, 214
Bsc4I CCNNNNNNNGG 6 cut(s) 77, 221, 293, 362, 602, 1680
Bse1I ACTGG 8 cut(s) 153, 205, 256, 751, 1092, 1189, 1494, 1550
Bse21I CCTNAGG 1 cut(s) 141
Bse3DI GCAATG 2 cut(s) 1227, 1468
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 2 cut(s) 679, 1448
BseCI ATCGAT 1 cut(s) 1688
BseDI CCNNGG 5 cut(s) 515, 690, 711, 788, 1402
BseGI GGATG 9 cut(s) 411, 607, 655, 859, 1128, 1190, 1456, 1663, 1787
BseLI CCNNNNNNNGG 6 cut(s) 77, 221, 293, 362, 602, 1680
BseMI GCAATG 2 cut(s) 1227, 1468
BseMII CTCAG 2 cut(s) 458, 1764
BseNI ACTGG 8 cut(s) 153, 205, 256, 751, 1092, 1189, 1494, 1550
BseRI GAGGAG 1 cut(s) 910
BseXI GCAGC 3 cut(s) 111, 1410, 1554
Bsh1236I CGCG 1 cut(s) 1369
BshFI GGCC 7 cut(s) 429, 606, 689, 964, 1510, 1550, 1684
BshVI ATCGAT 1 cut(s) 1688
BsiHKAI GWGCWC 4 cut(s) 722, 1026, 1109, 1167
BsiHKCI CYCGRG 2 cut(s) 13, 1233
BsiSI CCGG 2 cut(s) 43, 215
BsiWI CGTACG 1 cut(s) 397
BslFI GGGAC 2 cut(s) 632, 1165
BslI CCNNNNNNNGG 6 cut(s) 77, 221, 293, 362, 602, 1680
BsmAI GTCTC 1 cut(s) 10
BsmFI GGGAC 2 cut(s) 632, 1165
BsnI GGCC 7 cut(s) 429, 606, 689, 964, 1510, 1550, 1684
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 2 cut(s) 13, 1233
Bsp1286I GDGCHC 5 cut(s) 722, 1026, 1109, 1167, 1242
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 7 cut(s) 31, 82, 460, 553, 877, 969, 1594
Bsp1720I GCTNAGC 1 cut(s) 1773
Bsp19I CCATGG 1 cut(s) 711
BspACI CCGC 3 cut(s) 436, 1369, 1681
BspANI GGCC 7 cut(s) 429, 606, 689, 964, 1510, 1550, 1684
BspCNI CTCAG 2 cut(s) 457, 1765
BspDI ATCGAT 1 cut(s) 1688
BspEI TCCGGA 1 cut(s) 214
BspFNI CGCG 1 cut(s) 1369
BspLI GGNNCC 3 cut(s) 709, 919, 1596
BspMAI CTGCAG 2 cut(s) 574, 616
BspPI GGATC 3 cut(s) 548, 1589, 1602
BspQI GCTCTTC 1 cut(s) 993
BspTI CTTAAG 1 cut(s) 311
BspTNI GGTCTC 1 cut(s) 10
BsrBI CCGCTC 1 cut(s) 438
BsrDI GCAATG 2 cut(s) 1227, 1468
BsrI ACTGG 8 cut(s) 153, 205, 256, 751, 1092, 1189, 1494, 1550
BssECI CCNNGG 5 cut(s) 515, 690, 711, 788, 1402
BssMI GATC 7 cut(s) 31, 82, 460, 553, 877, 969, 1594
BssSI CACGAG 1 cut(s) 1067
BssT1I CCWWGG 4 cut(s) 690, 711, 788, 1402
Bst2BI CACGAG 1 cut(s) 1067
Bst2UI CCWGG 2 cut(s) 679, 1448
Bst4CI ACNGT 2 cut(s) 352, 1158
Bst6I CTCTTC 1 cut(s) 993
BstAFI CTTAAG 1 cut(s) 311
BstBAI YACGTR 1 cut(s) 401
BstC8I GCNNGC 3 cut(s) 675, 1441, 1779
BstDEI CTNAG 4 cut(s) 141, 444, 495, 1773
BstDSI CCRYGG 1 cut(s) 711
BstENI CCTNNNNNAGG 1 cut(s) 600
BstF5I GGATG 9 cut(s) 411, 607, 655, 859, 1128, 1190, 1456, 1663, 1787
BstFNI CGCG 1 cut(s) 1369
BstHHI GCGC 3 cut(s) 6, 1209, 1369
BstKTI GATC 7 cut(s) 34, 85, 463, 556, 880, 972, 1597
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 7 cut(s) 31, 82, 460, 553, 877, 969, 1594
BstMWI GCNNNNNNNGC 4 cut(s) 435, 679, 1690, 1778
BstNI CCWGG 2 cut(s) 679, 1448
BstSCI CCNGG 2 cut(s) 677, 1446
BstSFI CTRYAG 2 cut(s) 570, 612
BstUI CGCG 1 cut(s) 1369
BstV1I GCAGC 3 cut(s) 111, 1410, 1554
BstV2I GAAGAC 3 cut(s) 743, 903, 1361
BstX2I RGATCY 3 cut(s) 553, 877, 1594
BstXI CCANNNNNNTGG 1 cut(s) 263
BstYI RGATCY 3 cut(s) 553, 877, 1594
Bsu15I ATCGAT 1 cut(s) 1688
Bsu36I CCTNAGG 1 cut(s) 141
BsuI GTATCC 2 cut(s) 299, 1670
BsuRI GGCC 7 cut(s) 429, 606, 689, 964, 1510, 1550, 1684
BsuTUI ATCGAT 1 cut(s) 1688
BtgI CCRYGG 1 cut(s) 711
BtrI CACGTC 2 cut(s) 666, 1576
BtsCI GGATG 9 cut(s) 411, 607, 655, 859, 1128, 1190, 1456, 1663, 1787
BtsIMutI CAGTG 3 cut(s) 198, 744, 1154
Cac8I GCNNGC 3 cut(s) 675, 1441, 1779
CaiI CAGNNNCTG 1 cut(s) 806
CfoI GCGC 3 cut(s) 6, 1209, 1369
Cfr13I GGNCC 4 cut(s) 203, 814, 962, 1548
ClaI ATCGAT 1 cut(s) 1688
CseI GACGC 1 cut(s) 554
Csp6I GTAC 3 cut(s) 68, 398, 1481
CviAII CATG 9 cut(s) 21, 113, 712, 952, 1210, 1255, 1586, 1708, 1767
CviQI GTAC 3 cut(s) 68, 398, 1481
DdeI CTNAG 4 cut(s) 141, 444, 495, 1773
DpnI GATC 7 cut(s) 33, 84, 462, 555, 879, 971, 1596
DpnII GATC 7 cut(s) 31, 82, 460, 553, 877, 969, 1594
DrdI GACNNNNNNGTC 1 cut(s) 1051
DseDI GACNNNNNNGTC 1 cut(s) 1051
EaeI YGGCCR 1 cut(s) 1682
Eam1104I CTCTTC 1 cut(s) 993
EarI CTCTTC 1 cut(s) 993
Ecl136II GAGCTC 1 cut(s) 1024
Eco130I CCWWGG 4 cut(s) 690, 711, 788, 1402
Eco147I AGGCCT 2 cut(s) 429, 1510
Eco24I GRGCYC 2 cut(s) 1026, 1242
Eco31I GGTCTC 1 cut(s) 10
Eco32I GATATC 1 cut(s) 981
Eco47I GGWCC 2 cut(s) 203, 814
Eco53kI GAGCTC 1 cut(s) 1024
Eco57I CTGAAG 2 cut(s) 737, 886
Eco81I CCTNAGG 1 cut(s) 141
Eco88I CYCGRG 2 cut(s) 13, 1233
EcoICRI GAGCTC 1 cut(s) 1024
EcoNI CCTNNNNNAGG 1 cut(s) 600
EcoRII CCWGG 2 cut(s) 677, 1446
EcoRV GATATC 1 cut(s) 981
EcoT14I CCWWGG 4 cut(s) 690, 711, 788, 1402
EcoT38I GRGCYC 2 cut(s) 1026, 1242
ErhI CCWWGG 4 cut(s) 690, 711, 788, 1402
FaeI CATG 9 cut(s) 24, 116, 715, 955, 1213, 1258, 1589, 1711, 1770
FalI AAGNNNNNCTT 2 cut(s) 1545, 1577
FaqI GGGAC 2 cut(s) 632, 1165
FatI CATG 9 cut(s) 20, 112, 711, 951, 1209, 1254, 1585, 1707, 1766
FblI GTMKAC 1 cut(s) 1542
Fnu4HI GCNGC 5 cut(s) 125, 436, 1399, 1568, 1682
FokI GGATG 9 cut(s) 418, 594, 642, 866, 1135, 1197, 1463, 1650, 1774
FriOI GRGCYC 2 cut(s) 1026, 1242
Fsp4HI GCNGC 5 cut(s) 125, 436, 1399, 1568, 1682
FspBI CTAG 3 cut(s) 384, 522, 1296
GlaI GCGC 3 cut(s) 5, 1208, 1368
GluI GCNGC 5 cut(s) 125, 436, 1399, 1568, 1682
GsuI CTGGAG 2 cut(s) 239, 527
HaeIII GGCC 7 cut(s) 429, 606, 689, 964, 1510, 1550, 1684
HapII CCGG 2 cut(s) 43, 215
HgaI GACGC 1 cut(s) 554
HhaI GCGC 3 cut(s) 6, 1209, 1369
Hin1II CATG 9 cut(s) 24, 116, 715, 955, 1213, 1258, 1589, 1711, 1770
Hin6I GCGC 3 cut(s) 4, 1207, 1367
HinP1I GCGC 3 cut(s) 4, 1207, 1367
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 6 cut(s) 11, 375, 389, 836, 864, 1063
HpaI GTTAAC 1 cut(s) 619
HpaII CCGG 2 cut(s) 43, 215
HphI GGTGA 4 cut(s) 79, 92, 915, 1054
Hpy166II GTNNAC 3 cut(s) 619, 940, 1543
Hpy188I TCNGA 7 cut(s) 516, 558, 756, 841, 1035, 1285, 1375
Hpy8I GTNNAC 3 cut(s) 619, 940, 1543
HpyAV CCTTC 5 cut(s) 100, 606, 844, 1175, 1178
HpyCH4III ACNGT 2 cut(s) 352, 1158
HpyCH4IV ACGT 5 cut(s) 237, 396, 400, 665, 1575
HpyF10VI GCNNNNNNNGC 4 cut(s) 435, 679, 1690, 1778
HpyF3I CTNAG 4 cut(s) 141, 444, 495, 1773
HpySE526I ACGT 5 cut(s) 237, 396, 400, 665, 1575
Hsp92II CATG 9 cut(s) 24, 116, 715, 955, 1213, 1258, 1589, 1711, 1770
HspAI GCGC 3 cut(s) 4, 1207, 1367
Kpn2I TCCGGA 1 cut(s) 214
KspAI GTTAAC 1 cut(s) 619
Kzo9I GATC 7 cut(s) 31, 82, 460, 553, 877, 969, 1594
LguI GCTCTTC 1 cut(s) 993
LmnI GCTCC 2 cut(s) 217, 923
Lsp1109I GCAGC 3 cut(s) 111, 1410, 1554
LweI GCATC 5 cut(s) 1113, 1175, 1569, 1680, 1763
MaeI CTAG 3 cut(s) 384, 522, 1296
MaeII ACGT 5 cut(s) 237, 396, 400, 665, 1575
MaeIII GTNAC 4 cut(s) 700, 742, 1042, 1083
MalI GATC 7 cut(s) 33, 84, 462, 555, 879, 971, 1596
MbiI CCGCTC 1 cut(s) 438
MboI GATC 7 cut(s) 31, 82, 460, 553, 877, 969, 1594
MboII GAAGA 9 cut(s) 503, 743, 765, 805, 908, 1010, 1366, 1412, 1749
MflI RGATCY 3 cut(s) 553, 877, 1594
MhlI GDGCHC 5 cut(s) 722, 1026, 1109, 1167, 1242
MlyI GAGTC 2 cut(s) 5, 1057
MmeI TCCRAC 2 cut(s) 1178, 1331
MroI TCCGGA 1 cut(s) 214
MroXI GAANNNNTTC 1 cut(s) 1610
MseI TTAA 8 cut(s) 158, 312, 449, 618, 993, 1193, 1289, 1749
MslI CAYNNNNRTG 2 cut(s) 1449, 1733
MspCI CTTAAG 1 cut(s) 311
MspI CCGG 2 cut(s) 43, 215
MspR9I CCNGG 2 cut(s) 679, 1448
MvaI CCWGG 2 cut(s) 679, 1448
MvnI CGCG 1 cut(s) 1369
MwoI GCNNNNNNNGC 4 cut(s) 435, 679, 1690, 1778
NcoI CCATGG 1 cut(s) 711
NdeII GATC 7 cut(s) 31, 82, 460, 553, 877, 969, 1594
NlaIII CATG 9 cut(s) 24, 116, 715, 955, 1213, 1258, 1589, 1711, 1770
NlaIV GGNNCC 3 cut(s) 709, 919, 1596
NmuCI GTSAC 3 cut(s) 700, 742, 1042
OliI CACNNNNGTG 1 cut(s) 1733
PaeR7I CTCGAG 1 cut(s) 13
PceI AGGCCT 2 cut(s) 429, 1510
PciSI GCTCTTC 1 cut(s) 993
PdmI GAANNNNTTC 1 cut(s) 1610
PfeI GAWTC 4 cut(s) 375, 389, 836, 864
Pfl23II CGTACG 1 cut(s) 397
PkrI GCNGC 5 cut(s) 126, 437, 1400, 1569, 1683
PleI GAGTC 2 cut(s) 5, 1057
PpsI GAGTC 2 cut(s) 5, 1057
Ppu21I YACGTR 1 cut(s) 401
PsiI TTATAA 1 cut(s) 27
Psp124BI GAGCTC 1 cut(s) 1026
Psp1406I AACGTT 1 cut(s) 237
Psp6I CCWGG 2 cut(s) 677, 1446
PspGI CCWGG 2 cut(s) 677, 1446
PspLI CGTACG 1 cut(s) 397
PspN4I GGNNCC 3 cut(s) 709, 919, 1596
PspPI GGNCC 4 cut(s) 203, 814, 962, 1548
PstI CTGCAG 2 cut(s) 574, 616
PstNI CAGNNNCTG 1 cut(s) 806
PsuI RGATCY 3 cut(s) 553, 877, 1594
RsaI GTAC 3 cut(s) 69, 399, 1482
RsaNI GTAC 3 cut(s) 68, 398, 1481
RseI CAYNNNNRTG 2 cut(s) 1449, 1733
SacI GAGCTC 1 cut(s) 1026
SapI GCTCTTC 1 cut(s) 993
SaqAI TTAA 8 cut(s) 158, 312, 449, 618, 993, 1193, 1289, 1749
SatI GCNGC 5 cut(s) 125, 436, 1399, 1568, 1682
Sau3AI GATC 7 cut(s) 31, 82, 460, 553, 877, 969, 1594
Sau96I GGNCC 4 cut(s) 203, 814, 962, 1548
SchI GAGTC 2 cut(s) 5, 1057
ScrFI CCNGG 2 cut(s) 679, 1448
SduI GDGCHC 5 cut(s) 722, 1026, 1109, 1167, 1242
SfaNI GCATC 5 cut(s) 1113, 1175, 1569, 1680, 1763
SfcI CTRYAG 2 cut(s) 570, 612
Sfr274I CTCGAG 1 cut(s) 13
SinI GGWCC 2 cut(s) 203, 814
SlaI CTCGAG 1 cut(s) 13
SmiMI CAYNNNNRTG 2 cut(s) 1449, 1733
SmlI CTYRAG 8 cut(s) 13, 311, 1019, 1241, 1262, 1562, 1628, 1742
SmoI CTYRAG 8 cut(s) 13, 311, 1019, 1241, 1262, 1562, 1628, 1742
SseBI AGGCCT 2 cut(s) 429, 1510
SsiI CCGC 3 cut(s) 436, 1369, 1681
SspI AATATT 2 cut(s) 135, 910
SspMI CTAG 3 cut(s) 384, 522, 1296
SstI GAGCTC 1 cut(s) 1026
StuI AGGCCT 2 cut(s) 429, 1510
StyD4I CCNGG 2 cut(s) 677, 1446
StyI CCWWGG 4 cut(s) 690, 711, 788, 1402
TaaI ACNGT 2 cut(s) 352, 1158
TaiI ACGT 5 cut(s) 240, 399, 403, 668, 1578
TaqI TCGA 4 cut(s) 14, 260, 1688, 1725
TauI GCSGC 2 cut(s) 438, 1684
TfiI GAWTC 4 cut(s) 375, 389, 836, 864
Tru1I TTAA 8 cut(s) 158, 312, 449, 618, 993, 1193, 1289, 1749
Tru9I TTAA 8 cut(s) 158, 312, 449, 618, 993, 1193, 1289, 1749
TscAI CASTG 3 cut(s) 205, 751, 1161
TseFI GTSAC 3 cut(s) 700, 742, 1042
TseI GCWGC 3 cut(s) 124, 1398, 1567
Tsp45I GTSAC 3 cut(s) 700, 742, 1042
TspDTI ATGAA 6 cut(s) 37, 207, 968, 1160, 1226, 1776
TspRI CASTG 3 cut(s) 205, 751, 1161
Vha464I CTTAAG 1 cut(s) 311
VpaK11BI GGWCC 2 cut(s) 203, 814
XagI CCTNNNNNAGG 1 cut(s) 600
XapI RAATTY 2 cut(s) 1291, 1426
XhoI CTCGAG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 1542
XmnI GAANNNNTTC 1 cut(s) 1610
XspI CTAG 3 cut(s) 384, 522, 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.