pycom14g15390

Nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002055
Physical Location & Seq
Forward (+)
18020453 .. 18024631
4179 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1614 bp
ATGGCGAGTGGAGGCGGCGGTGGAGCTCCAAAAGTTACTGAGCCACAGCCACATCCACCAAAAGAACAGCTAGCCAACGTTTCTTACTGCATTACTAGTCCACCTCCATGGCCCGATGCCATTCTCCTTGGTTTTCAACACTACATTGTGATGCTTGGAACTTCAGTTCTCATCCCCAGTGCTCTTGTTCCCCAGATGGGAGGAGGAAATGAGGAGAAGGCCAAGGTGATCCAGACGCTACTCTTTGTTGCTGGTTTGAACACATTGAAACAGACTTTGTTTGGGACTAGACTACCAGCAGTGATTGGAGGGTCATATACGTTTGTCCCGACCACAATTTCCATCATATTAGCTGGTCGATTCAGTGATGGTCAAGACCCTATAGAGAAATTTAAGAGGACAATGCGGGCAATCCAGGGTGCTCTTATTGTTGCTTCAACTCTTCAGATAGTCTTAGGCTTCAGTGGCCTTTGGCGTAATGTTGCAAGGTTTTTAAGCCCACTTTCAGTTGTCCCTTTGGTAGGAATGGTTGGTTTTGGACTCTACGAGTTAGGTTTTCCAGTGCTTGCAAAATGTGTGGAGATTGGACTGCCAGAGCTTATCATTTTAGTATTCATTTCTCAGGGAAAATTATTCTGCAAGGCTGATATTGTATTTGCAATGATTGTGATGCAGTATTTGCCGCACATAATTCCCAGAGGAAAACGTATATTTGATCGTTTTGCTGTCATATTTTCTGTGGTGATTGTGTGGATTTATGCTCACCTACTCACTGTTGGTGGAGCATATAATGATACTGGCCCAAAAACACAGGCAAGCTGCCGTACTGATCGTGCTGGACTTATAAATGCTGCTCCGTGGATTAGAGTTCCATATCCCTTCCAATGGGGAGCACCTTCCTTTGATGCTGGTGAAGCCTTTGCTATGATGGCAGCGTCTTTCGTTGCACTTGTTGAGTCAACTGGTGCTTTCATCGCTGTTTCAAGATATGCTAGTGCAACTCCAGTGCCACCTGCTATTCTGAGTCGTGGAGTTGGTTGGCAGGGAGTTGGCATTCTGATTTCGGGCTTGTTTGGAACTGTGACCGGATCCTCAGTATCTGTTCGAAGGGTTGTTCAAATATCTGCTGGTTTCATGATATTTTTCTCCATTCTGGGAAAATTTGGAGCAGTCTTCGCATCGATCCCAGGGCCAATCATTGCTGCTTTGTACTGTCTGTTCTTTGCATATGTTGGTGCCGGAGGTCTTAGCTTTCTACAATTCTGTAACCTAAACAGCTTCCGAACAAAGTTCATATTGGGCTTCTCTATCTTCCTTGGTTTGTCCGTGCCACAGTACTTCAACGAGTACACAGCCATCAACGGTTACGGTCCAGTCCACACAGGAGGAAGATGGTTCAATGATATAATCAATGTCCCTCTACAGTCAGAAGCGTTTGTCGCCGGCTGTGTCGCATATTTCTTGGACAACACACTGCAGCGGAAGGACAGTACAGTTAGGAAAGACCGAGGTAAACATTGGTGGGACAAGTTCCGAACCTTCAAGGGAGAATCAAGGAGTGAGGAGTTTTATTCGCTACCATTCAATCTCAACAAGTATTTTCCATCCGTATGA

Protein Analysis

538

Amino Acids

58.29

Weight (kDa)

9.49

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 40 - 447 1.3e-51 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 845
AarI CACCTGC 1 cut(s) 1021
Acc36I ACCTGC 1 cut(s) 1021
AccB1I GGYRCC 1 cut(s) 1235
AciI CCGC 5 cut(s) 15, 18, 406, 683, 1480
AclI AACGTT 1 cut(s) 78
AclWI GGATC 4 cut(s) 223, 1083, 1096, 1177
AcsI RAATTY 2 cut(s) 389, 1160
AcuI CTGAAG 3 cut(s) 147, 428, 445
AfaI GTAC 5 cut(s) 826, 1211, 1337, 1349, 1492
AfiI CCNNNNNNNGG 3 cut(s) 197, 521, 885
AhlI ACTAGT 1 cut(s) 95
AjnI CCWGG 2 cut(s) 414, 1186
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
AluBI AGCT 7 cut(s) 26, 70, 353, 598, 819, 1251, 1278
AluI AGCT 7 cut(s) 26, 70, 353, 598, 819, 1251, 1278
Alw21I GWGCWC 4 cut(s) 28, 184, 424, 895
AlwI GGATC 4 cut(s) 223, 1083, 1096, 1177
AlwNI CAGNNNCTG 1 cut(s) 1100
AoxI GGCC 5 cut(s) 110, 219, 466, 799, 1190
ApeKI GCWGC 5 cut(s) 819, 851, 932, 1202, 1477
ApoI RAATTY 2 cut(s) 389, 1160
AspS9I GGNCC 4 cut(s) 111, 800, 1190, 1370
AsuHPI GGTGA 4 cut(s) 238, 754, 755, 923
AsuII TTCGAA 1 cut(s) 1105
AsuNHI GCTAGC 1 cut(s) 70
AvaII GGWCC 1 cut(s) 1370
BamHI GGATCC 1 cut(s) 1088
BanI GGYRCC 1 cut(s) 1235
BanII GRGCYC 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 1165
Bbv12I GWGCWC 4 cut(s) 28, 184, 424, 895
BbvI GCAGC 5 cut(s) 806, 838, 944, 1189, 1489
BccI CCATC 7 cut(s) 190, 350, 362, 922, 1364, 1386, 1612
BceAI ACGGC 1 cut(s) 807
BciT130I CCWGG 2 cut(s) 416, 1188
BcuI ACTAGT 1 cut(s) 95
BfaI CTAG 4 cut(s) 71, 96, 288, 993
BfmI CTRYAG 3 cut(s) 381, 1421, 1475
BfuAI ACCTGC 1 cut(s) 1021
BisI GCNGC 7 cut(s) 16, 683, 820, 852, 933, 1203, 1478
BlsI GCNGC 7 cut(s) 17, 684, 821, 853, 934, 1204, 1479
BmcAI AGTACT 1 cut(s) 1337
Bme1390I CCNGG 2 cut(s) 416, 1188
Bme18I GGWCC 1 cut(s) 1370
BmgT120I GGNCC 4 cut(s) 111, 800, 1190, 1370
BmiI GGNNCC 2 cut(s) 1090, 1237
BmrFI CCNGG 2 cut(s) 416, 1188
BmrI ACTGGG 1 cut(s) 171
BmsI GCATC 5 cut(s) 106, 141, 660, 895, 1187
BmtI GCTAGC 1 cut(s) 74
BmuI ACTGGG 1 cut(s) 171
BpiI GAAGAC 1 cut(s) 1165
BpmI CTGGAG 1 cut(s) 987
Bpu14I TTCGAA 1 cut(s) 1105
Bsa29I ATCGAT 1 cut(s) 1181
BsaJI CCNNGG 9 cut(s) 107, 127, 222, 415, 857, 1186, 1187, 1315, 1507
BsaWI WCCGGW 1 cut(s) 1085
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 521, 885
Bse118I RCCGGY 1 cut(s) 1442
Bse1I ACTGG 6 cut(s) 177, 560, 802, 967, 1004, 1373
Bse3DI GCAATG 2 cut(s) 666, 1197
BseBI CCWGG 2 cut(s) 416, 1188
BseCI ATCGAT 1 cut(s) 1181
BseDI CCNNGG 9 cut(s) 107, 127, 222, 415, 857, 1186, 1187, 1315, 1507
BseGI GGATG 3 cut(s) 52, 171, 1604
BseLI CCNNNNNNNGG 3 cut(s) 197, 521, 885
BseMI GCAATG 2 cut(s) 666, 1197
BseMII CTCAG 4 cut(s) 30, 635, 1013, 1107
BseNI ACTGG 6 cut(s) 177, 560, 802, 967, 1004, 1373
BseRI GAGGAG 3 cut(s) 216, 227, 1577
BseXI GCAGC 5 cut(s) 806, 838, 944, 1189, 1489
BshFI GGCC 5 cut(s) 112, 221, 468, 801, 1192
BshNI GGYRCC 1 cut(s) 1235
BshVI ATCGAT 1 cut(s) 1181
BsiHKAI GWGCWC 4 cut(s) 28, 184, 424, 895
BsiSI CCGG 3 cut(s) 1086, 1239, 1443
BslFI GGGAC 5 cut(s) 298, 311, 497, 1400, 1538
BslI CCNNNNNNNGG 3 cut(s) 197, 521, 885
BsmFI GGGAC 5 cut(s) 298, 311, 497, 1400, 1538
BsmI GAATGC 1 cut(s) 1053
BsnI GGCC 5 cut(s) 112, 221, 468, 801, 1192
Bsp119I TTCGAA 1 cut(s) 1105
Bsp1286I GDGCHC 4 cut(s) 28, 184, 424, 895
Bsp143I GATC 5 cut(s) 228, 715, 829, 1088, 1182
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 5 cut(s) 15, 18, 406, 683, 1480
BspANI GGCC 5 cut(s) 112, 221, 468, 801, 1192
BspCNI CTCAG 4 cut(s) 31, 634, 1014, 1106
BspDI ATCGAT 1 cut(s) 1181
BspHI TCATGA 1 cut(s) 1134
BspLI GGNNCC 2 cut(s) 1090, 1237
BspMAI CTGCAG 1 cut(s) 1479
BspMI ACCTGC 1 cut(s) 1021
BspOI GCTAGC 1 cut(s) 74
BspPI GGATC 4 cut(s) 223, 1083, 1096, 1177
BspT104I TTCGAA 1 cut(s) 1105
BspT107I GGYRCC 1 cut(s) 1235
BsrDI GCAATG 2 cut(s) 666, 1197
BsrFI RCCGGY 1 cut(s) 1442
BsrI ACTGG 6 cut(s) 177, 560, 802, 967, 1004, 1373
BssAI RCCGGY 1 cut(s) 1442
BssECI CCNNGG 9 cut(s) 107, 127, 222, 415, 857, 1186, 1187, 1315, 1507
BssMI GATC 5 cut(s) 228, 715, 829, 1088, 1182
BssT1I CCWWGG 4 cut(s) 107, 127, 222, 1315
Bst2UI CCWGG 2 cut(s) 416, 1188
Bst4CI ACNGT 9 cut(s) 775, 1081, 1214, 1335, 1364, 1370, 1425, 1490, 1495
Bst6I CTCTTC 1 cut(s) 447
BstAPI GCANNNNNTGC 1 cut(s) 679
BstBI TTCGAA 1 cut(s) 1105
BstC8I GCNNGC 5 cut(s) 72, 408, 567, 817, 1444
BstDEI CTNAG 6 cut(s) 39, 454, 621, 1022, 1093, 1247
BstDSI CCRYGG 2 cut(s) 107, 857
BstENI CCTNNNNNAGG 1 cut(s) 519
BstF5I GGATG 3 cut(s) 52, 171, 1604
BstKTI GATC 5 cut(s) 231, 718, 832, 1091, 1185
BstMBI GATC 5 cut(s) 228, 715, 829, 1088, 1182
BstMWI GCNNNNNNNGC 7 cut(s) 465, 679, 914, 929, 974, 1175, 1439
BstNI CCWGG 2 cut(s) 416, 1188
BstSCI CCNGG 2 cut(s) 414, 1186
BstSFI CTRYAG 3 cut(s) 381, 1421, 1475
BstV1I GCAGC 5 cut(s) 806, 838, 944, 1189, 1489
BstV2I GAAGAC 1 cut(s) 1165
BstX2I RGATCY 1 cut(s) 1088
BstXI CCANNNNNNTGG 1 cut(s) 108
BstYI RGATCY 1 cut(s) 1088
Bsu15I ATCGAT 1 cut(s) 1181
BsuRI GGCC 5 cut(s) 112, 221, 468, 801, 1192
BsuTUI ATCGAT 1 cut(s) 1181
BtgI CCRYGG 2 cut(s) 107, 857
BtgZI GCGATG 1 cut(s) 958
BtsCI GGATG 3 cut(s) 52, 171, 1604
BtsI GCAGTG 2 cut(s) 306, 1472
BtsIMutI CAGTG 8 cut(s) 184, 306, 370, 469, 567, 771, 1011, 1472
BveI ACCTGC 1 cut(s) 1021
Cac8I GCNNGC 5 cut(s) 72, 408, 567, 817, 1444
CaiI CAGNNNCTG 1 cut(s) 1100
CciI TCATGA 1 cut(s) 1134
Cfr10I RCCGGY 1 cut(s) 1442
Cfr13I GGNCC 4 cut(s) 111, 800, 1190, 1370
ClaI ATCGAT 1 cut(s) 1181
CseI GACGC 2 cut(s) 244, 924
Csp6I GTAC 5 cut(s) 825, 1210, 1336, 1348, 1491
CspCI CAANNNNNGTGG 2 cut(s) 558, 593
CviAII CATG 2 cut(s) 108, 1135
CviQI GTAC 5 cut(s) 825, 1210, 1336, 1348, 1491
DdeI CTNAG 6 cut(s) 39, 454, 621, 1022, 1093, 1247
DpnI GATC 5 cut(s) 230, 717, 831, 1090, 1184
DpnII GATC 5 cut(s) 228, 715, 829, 1088, 1182
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 4 cut(s) 107, 127, 222, 1315
Eco24I GRGCYC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 1370
Eco53kI GAGCTC 1 cut(s) 26
Eco57I CTGAAG 3 cut(s) 147, 428, 445
EcoICRI GAGCTC 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 519
EcoRII CCWGG 2 cut(s) 414, 1186
EcoT14I CCWWGG 4 cut(s) 107, 127, 222, 1315
EcoT38I GRGCYC 1 cut(s) 28
ErhI CCWWGG 4 cut(s) 107, 127, 222, 1315
FaeI CATG 2 cut(s) 111, 1138
FaqI GGGAC 5 cut(s) 298, 311, 497, 1400, 1538
FatI CATG 2 cut(s) 107, 1134
FauI CCCGC 1 cut(s) 399
FauNDI CATATG 1 cut(s) 1228
Fnu4HI GCNGC 7 cut(s) 16, 683, 820, 852, 933, 1203, 1478
FokI GGATG 3 cut(s) 39, 158, 1591
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 7 cut(s) 16, 683, 820, 852, 933, 1203, 1478
FspBI CTAG 4 cut(s) 71, 96, 288, 993
GluI GCNGC 7 cut(s) 16, 683, 820, 852, 933, 1203, 1478
GsuI CTGGAG 1 cut(s) 987
HaeIII GGCC 5 cut(s) 112, 221, 468, 801, 1192
HapII CCGG 3 cut(s) 1086, 1239, 1443
HgaI GACGC 2 cut(s) 244, 924
Hin1II CATG 2 cut(s) 111, 1138
HincII GTYRAC 1 cut(s) 960
HindII GTYRAC 1 cut(s) 960
HinfI GANTC 5 cut(s) 360, 540, 956, 1024, 1550
HpaII CCGG 3 cut(s) 1086, 1239, 1443
HphI GGTGA 4 cut(s) 238, 754, 755, 923
Hpy166II GTNNAC 5 cut(s) 101, 960, 1350, 1378, 1514
Hpy188I TCNGA 6 cut(s) 447, 1023, 1059, 1283, 1429, 1535
Hpy188III TCNNGA 5 cut(s) 232, 328, 374, 984, 1135
Hpy8I GTNNAC 5 cut(s) 101, 960, 1350, 1378, 1514
HpyAV CCTTC 6 cut(s) 211, 889, 906, 1101, 1477, 1549
HpyCH4III ACNGT 9 cut(s) 775, 1081, 1214, 1335, 1364, 1370, 1425, 1490, 1495
HpyCH4IV ACGT 3 cut(s) 78, 320, 706
HpyF10VI GCNNNNNNNGC 7 cut(s) 465, 679, 914, 929, 974, 1175, 1439
HpyF3I CTNAG 6 cut(s) 39, 454, 621, 1022, 1093, 1247
HpySE526I ACGT 3 cut(s) 78, 320, 706
Hsp92II CATG 2 cut(s) 111, 1138
KroI GCCGGC 1 cut(s) 1442
KroNI GCCGGC 1 cut(s) 1444
Kzo9I GATC 5 cut(s) 228, 715, 829, 1088, 1182
LmnI GCTCC 6 cut(s) 23, 31, 782, 859, 890, 1166
Lsp1109I GCAGC 5 cut(s) 806, 838, 944, 1189, 1489
LweI GCATC 5 cut(s) 106, 141, 660, 895, 1187
MaeI CTAG 4 cut(s) 71, 96, 288, 993
MaeII ACGT 3 cut(s) 78, 320, 706
MaeIII GTNAC 4 cut(s) 34, 1081, 1265, 1364
MalI GATC 5 cut(s) 230, 717, 831, 1090, 1184
MboI GATC 5 cut(s) 228, 715, 829, 1088, 1182
MboII GAAGA 4 cut(s) 434, 1165, 1303, 1401
MflI RGATCY 1 cut(s) 1088
MhlI GDGCHC 4 cut(s) 28, 184, 424, 895
MluCI AATT 6 cut(s) 336, 389, 629, 690, 1160, 1259
MlyI GAGTC 3 cut(s) 534, 965, 1033
MroNI GCCGGC 1 cut(s) 1442
MseI TTAA 2 cut(s) 393, 494
MslI CAYNNNNRTG 3 cut(s) 106, 149, 1609
MspA1I CMGCKG 1 cut(s) 1480
MspI CCGG 3 cut(s) 1086, 1239, 1443
MspR9I CCNGG 2 cut(s) 416, 1188
Mva1269I GAATGC 1 cut(s) 1053
MvaI CCWGG 2 cut(s) 416, 1188
MwoI GCNNNNNNNGC 7 cut(s) 465, 679, 914, 929, 974, 1175, 1439
NaeI GCCGGC 1 cut(s) 1444
NcoI CCATGG 1 cut(s) 107
NdeI CATATG 1 cut(s) 1228
NdeII GATC 5 cut(s) 228, 715, 829, 1088, 1182
NgoMIV GCCGGC 1 cut(s) 1442
NheI GCTAGC 1 cut(s) 70
NlaIII CATG 2 cut(s) 111, 1138
NlaIV GGNNCC 2 cut(s) 1090, 1237
NmuCI GTSAC 1 cut(s) 1081
NspV TTCGAA 1 cut(s) 1105
PagI TCATGA 1 cut(s) 1134
PaqCI CACCTGC 1 cut(s) 1021
PasI CCCWGGG 1 cut(s) 1187
PcsI WCGNNNNNNNCGW 1 cut(s) 326
PctI GAATGC 1 cut(s) 1053
PdiI GCCGGC 1 cut(s) 1444
PfeI GAWTC 2 cut(s) 360, 1550
PkrI GCNGC 7 cut(s) 17, 684, 821, 853, 934, 1204, 1479
PleI GAGTC 3 cut(s) 534, 964, 1032
PpsI GAGTC 3 cut(s) 534, 964, 1032
PsiI TTATAA 1 cut(s) 845
Psp124BI GAGCTC 1 cut(s) 28
Psp1406I AACGTT 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 414, 1186
PspGI CCWGG 2 cut(s) 414, 1186
PspN4I GGNNCC 2 cut(s) 1090, 1237
PspPI GGNCC 4 cut(s) 111, 800, 1190, 1370
PsrI GAACNNNNNNTAC 2 cut(s) 1202, 1234
PstI CTGCAG 1 cut(s) 1479
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 1 cut(s) 1088
RsaI GTAC 5 cut(s) 826, 1211, 1337, 1349, 1492
RsaNI GTAC 5 cut(s) 825, 1210, 1336, 1348, 1491
RseI CAYNNNNRTG 3 cut(s) 106, 149, 1609
SacI GAGCTC 1 cut(s) 28
SaqAI TTAA 2 cut(s) 393, 494
SatI GCNGC 7 cut(s) 16, 683, 820, 852, 933, 1203, 1478
Sau3AI GATC 5 cut(s) 228, 715, 829, 1088, 1182
Sau96I GGNCC 4 cut(s) 111, 800, 1190, 1370
ScaI AGTACT 1 cut(s) 1337
SchI GAGTC 3 cut(s) 534, 965, 1033
ScrFI CCNGG 2 cut(s) 416, 1188
SduI GDGCHC 4 cut(s) 28, 184, 424, 895
SfaNI GCATC 5 cut(s) 106, 141, 660, 895, 1187
SfcI CTRYAG 3 cut(s) 381, 1421, 1475
SfuI TTCGAA 1 cut(s) 1105
SinI GGWCC 1 cut(s) 1370
SmiMI CAYNNNNRTG 3 cut(s) 106, 149, 1609
SpeI ACTAGT 1 cut(s) 95
Sse9I AATT 6 cut(s) 336, 389, 629, 690, 1160, 1259
SsiI CCGC 5 cut(s) 15, 18, 406, 683, 1480
SspMI CTAG 4 cut(s) 71, 96, 288, 993
SstI GAGCTC 1 cut(s) 28
StyD4I CCNGG 2 cut(s) 414, 1186
StyI CCWWGG 4 cut(s) 107, 127, 222, 1315
TaaI ACNGT 9 cut(s) 775, 1081, 1214, 1335, 1364, 1370, 1425, 1490, 1495
TaiI ACGT 3 cut(s) 81, 323, 709
TaqI TCGA 3 cut(s) 358, 1105, 1181
TaqII GACCGA 1 cut(s) 1521
TasI AATT 6 cut(s) 336, 389, 629, 690, 1160, 1259
TatI WGTACW 4 cut(s) 1209, 1335, 1347, 1490
TauI GCSGC 2 cut(s) 18, 685
TfiI GAWTC 2 cut(s) 360, 1550
Tru1I TTAA 2 cut(s) 393, 494
Tru9I TTAA 2 cut(s) 393, 494
TscAI CASTG 8 cut(s) 184, 306, 370, 469, 567, 778, 1011, 1479
TseFI GTSAC 1 cut(s) 1081
TseI GCWGC 5 cut(s) 819, 851, 932, 1202, 1477
Tsp45I GTSAC 1 cut(s) 1081
TspDTI ATGAA 4 cut(s) 604, 961, 1123, 1282
TspGWI ACGGA 3 cut(s) 846, 1315, 1597
TspRI CASTG 8 cut(s) 184, 306, 370, 469, 567, 778, 1011, 1479
VpaK11BI GGWCC 1 cut(s) 1370
XagI CCTNNNNNAGG 1 cut(s) 519
XapI RAATTY 2 cut(s) 389, 1160
XspI CTAG 4 cut(s) 71, 96, 288, 993
ZrmI AGTACT 1 cut(s) 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.