pycom14g17230

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0021284
Physical Location & Seq
Forward (+)
19261181 .. 19261744
564 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g17230.2

Sequence Viewer

Length: 471 bp
ATGGGACAAGGAGTTCTTAGTGCTCACAACAACGGTTCAGCAGATTTACGCACTTATGCCCATTGTACAAGAGGAAGAGCCGGTATAACTCTACTCCTCATCAACTTGAGCAATGAGACTAATTTCATTACCAATGTTGAAAATATTTTGGACACCGATTTGGGTGCTCACGCAAGAAACATTAGCAAAGAAAGTTATTTCAAGCGCGGCCTGAAGAGAACGGTGTCATGGGTAGGACGCAAAGCATTAGATGAAGCAATTTGCAGAGAAGAGTACCATTTGACACCGAAAGATGGGGACCTCAGAAGCAAAACCATGATTCTCAACGGAGCGCCATTGGAGATCACCAAGGACGGAAACATCCCAGAATTGGAACCCGCTCGTGTTGACATGAAAGCTCCGTTAACAATCGCCCCGTTGTCCATCAAGTTTGTGGTGTTACCTTACGTCGATGCGCAAGCTTGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko05205 map00531 map01100 map05205
Pfam Domains
Protein Families
Glyco_hydro_79n

Protein Analysis

157

Amino Acids

17.16

Weight (kDa)

7.74

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021284)

Species Orthologous Gene IDs
pyrus_communis pycom14g17230
rosa_chinensis RchiOBHm_Chr7g0182501
rosa_samantha Rh7AG051400 Rh7CG052700 Rh7DG050400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 456
AccBSI CCGCTC 1 cut(s) 380
AccII CGCG 1 cut(s) 207
AciI CCGC 2 cut(s) 207, 378
AcuI CTGAAG 1 cut(s) 233
AfaI GTAC 2 cut(s) 67, 275
AfiI CCNNNNNNNGG 2 cut(s) 293, 370
AgsI TTSAA 2 cut(s) 140, 202
AloI GAACNNNNNNTCC 1 cut(s) 29
AluBI AGCT 2 cut(s) 398, 461
AluI AGCT 2 cut(s) 398, 461
Alw21I GWGCWC 2 cut(s) 25, 169
Alw26I GTCTC 1 cut(s) 110
AoxI GGCC 1 cut(s) 208
AspLEI GCGC 3 cut(s) 207, 334, 457
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 1 cut(s) 337
AvaII GGWCC 1 cut(s) 298
BauI CACGAG 1 cut(s) 381
Bbv12I GWGCWC 2 cut(s) 25, 169
BccI CCATC 2 cut(s) 287, 431
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BcoDI GTCTC 1 cut(s) 110
BfoI RGCGCY 1 cut(s) 335
BisI GCNGC 1 cut(s) 208
BlsI GCNGC 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 2 cut(s) 299, 375
BmsI GCATC 1 cut(s) 442
BpuEI CTTGAG 1 cut(s) 127
BsaJI CCNNGG 1 cut(s) 348
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 370
Bse118I RCCGGY 1 cut(s) 80
Bse3DI GCAATG 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 348
BseGI GGATG 1 cut(s) 360
BseLI CCNNNNNNNGG 2 cut(s) 293, 370
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 316
BseRI GAGGAG 1 cut(s) 86
Bsh1236I CGCG 1 cut(s) 207
BshFI GGCC 1 cut(s) 210
BsiHKAI GWGCWC 2 cut(s) 25, 169
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 2 cut(s) 18, 311
BslI CCNNNNNNNGG 2 cut(s) 293, 370
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 2 cut(s) 18, 311
BsnI GGCC 1 cut(s) 210
Bsp1286I GDGCHC 2 cut(s) 25, 169
Bsp1407I TGTACA 1 cut(s) 65
Bsp143I GATC 1 cut(s) 342
BspACI CCGC 2 cut(s) 207, 378
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 1 cut(s) 315
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 2 cut(s) 299, 375
BspQI GCTCTTC 1 cut(s) 70
BsrBI CCGCTC 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 1 cut(s) 342
BssSI CACGAG 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 381
Bst4CI ACNGT 2 cut(s) 35, 223
Bst6I CTCTTC 3 cut(s) 70, 209, 264
BstAUI TGTACA 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 2 cut(s) 17, 302
BstF5I GGATG 1 cut(s) 360
BstFNI CGCG 1 cut(s) 207
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 3 cut(s) 207, 334, 457
BstKTI GATC 1 cut(s) 345
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 342
BstUI CGCG 1 cut(s) 207
BsuRI GGCC 1 cut(s) 210
BtsCI GGATG 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 459
CfoI GCGC 3 cut(s) 207, 334, 457
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 298
CseI GACGC 1 cut(s) 246
Csp6I GTAC 2 cut(s) 66, 274
CviAII CATG 3 cut(s) 228, 316, 391
CviJI RGCY 4 cut(s) 80, 210, 398, 461
CviKI_1 RGCY 4 cut(s) 80, 210, 398, 461
CviQI GTAC 2 cut(s) 66, 274
DdeI CTNAG 2 cut(s) 17, 302
DpnI GATC 1 cut(s) 344
DpnII GATC 1 cut(s) 342
Eam1104I CTCTTC 3 cut(s) 70, 209, 264
EarI CTCTTC 3 cut(s) 70, 209, 264
Eco130I CCWWGG 1 cut(s) 348
Eco47I GGWCC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 3 cut(s) 231, 319, 394
FaiI YATR 6 cut(s) 57, 86, 229, 317, 392, 469
FalI AAGNNNNNCTT 1 cut(s) 32
FaqI GGGAC 2 cut(s) 18, 311
FatI CATG 3 cut(s) 227, 315, 390
FauI CCCGC 1 cut(s) 385
Fnu4HI GCNGC 1 cut(s) 208
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 208
FspI TGCGCA 1 cut(s) 456
GlaI GCGC 3 cut(s) 206, 333, 456
GluI GCNGC 1 cut(s) 208
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 81
HgaI GACGC 1 cut(s) 246
HhaI GCGC 3 cut(s) 207, 334, 457
Hin1II CATG 3 cut(s) 231, 319, 394
Hin6I GCGC 3 cut(s) 205, 332, 455
HinP1I GCGC 3 cut(s) 205, 332, 455
HincII GTYRAC 2 cut(s) 388, 405
HindII GTYRAC 2 cut(s) 388, 405
HindIII AAGCTT 1 cut(s) 459
HinfI GANTC 1 cut(s) 319
HpaI GTTAAC 1 cut(s) 405
HpaII CCGG 1 cut(s) 81
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 2 cut(s) 388, 405
Hpy188I TCNGA 1 cut(s) 305
Hpy8I GTNNAC 2 cut(s) 388, 405
Hpy99I CGWCG 1 cut(s) 452
HpyCH4III ACNGT 2 cut(s) 35, 223
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 1 cut(s) 264
HpyF3I CTNAG 2 cut(s) 17, 302
HpySE526I ACGT 1 cut(s) 447
Hsp92II CATG 3 cut(s) 231, 319, 394
HspAI GCGC 3 cut(s) 205, 332, 455
KspAI GTTAAC 1 cut(s) 405
Kzo9I GATC 1 cut(s) 342
LguI GCTCTTC 1 cut(s) 70
LmnI GCTCC 2 cut(s) 329, 403
LpnPI CCDG 3 cut(s) 94, 224, 378
LweI GCATC 1 cut(s) 442
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 1 cut(s) 438
MalI GATC 1 cut(s) 344
MbiI CCGCTC 1 cut(s) 380
MboI GATC 1 cut(s) 342
MboII GAAGA 3 cut(s) 87, 226, 281
MhlI GDGCHC 2 cut(s) 25, 169
MluCI AATT 3 cut(s) 121, 258, 368
MnlI CCTC 3 cut(s) 65, 107, 311
MseI TTAA 1 cut(s) 404
MspI CCGG 1 cut(s) 81
MvnI CGCG 1 cut(s) 207
NdeII GATC 1 cut(s) 342
NlaIII CATG 3 cut(s) 231, 319, 394
NlaIV GGNNCC 2 cut(s) 299, 375
NsbI TGCGCA 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 70
PfeI GAWTC 1 cut(s) 319
PkrI GCNGC 1 cut(s) 209
PpuMI RGGWCCY 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 298
PspN4I GGNNCC 2 cut(s) 299, 375
PspPI GGNCC 1 cut(s) 298
PspPPI RGGWCCY 1 cut(s) 298
RsaI GTAC 2 cut(s) 67, 275
RsaNI GTAC 2 cut(s) 66, 274
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 1 cut(s) 208
Sau3AI GATC 1 cut(s) 342
Sau96I GGNCC 1 cut(s) 298
SduI GDGCHC 2 cut(s) 25, 169
SetI ASST 5 cut(s) 303, 400, 445, 450, 463
SfaNI GCATC 1 cut(s) 442
SinI GGWCC 1 cut(s) 298
SmlI CTYRAG 1 cut(s) 106
SmoI CTYRAG 1 cut(s) 106
Sse9I AATT 3 cut(s) 121, 258, 368
SsiI CCGC 2 cut(s) 207, 378
SspI AATATT 1 cut(s) 145
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 2 cut(s) 35, 223
TaiI ACGT 1 cut(s) 450
TaqI TCGA 1 cut(s) 450
TasI AATT 3 cut(s) 121, 258, 368
TatI WGTACW 1 cut(s) 65
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 1 cut(s) 319
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TspDTI ATGAA 3 cut(s) 115, 267, 407
TspGWI ACGGA 3 cut(s) 342, 369, 390
VpaK11BI GGWCC 1 cut(s) 298
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.