pycom15g08470

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006801
Physical Location & Seq
Reverse (-)
5309508 .. 5314421
4914 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGGGTCGCCTAGTCCTCTCCCACTTCCTCACCGTCTCCACCGCCTCTTCTCCTCGCCTCGCATCTTCCCTGTGTGCTGGTGGATTTCGAATCCGGCAATGGAAAGCGCCTACTTCAATTCCGCAGATTCGGCTTCAAAGCGCGCGAAACGCCGGCGGTTTTCGGTGCTGCTGCAGCGTTTCGCTCTCGCAGCCCGCTGCTCCGGAGACTTCTCCCAGCTCTGTAAAGAGGAGGATAGTGTCTGGAGTCCAACCGACGGGTTCAATCCACCTGGGAAATTATCTTGGCGCCATAAGAAACTGGATTTTGCTACAGAATACATATGATACTCTCTTTTTCATTGTGGACCTTCATGCGATTACATTACCGTATGATGCACAACAACTATCCAAGGCGACAAGGGATACAGCAGCTCTTTATTTGGCATGTGGAGTGGATACCTCCAAGGCGTCTGTCTTTGTGCAGTCTCATGTTCGTGCCCATGCAGAAATGATGTGGCTTTTAAGTTCTGTCACGCCTGTCGGTTGGCTAAACAGAATGATTCAGTTTAAAGAGAAATCACGCAAGGCGGGGGACGAAAATGTTGGTGTTGGTCTTTTGACTTACCCTGTTTTGATGGCTTCTGATATTCTTTTATATCAGTCTGACTTTGTCCCGGTTGGTGAAGATCAAAAGCAGCATTTGGAGTTGACACGTGAACTGGCTGAGCGTGTTCCTGAGGCTCTTATACCACCAGTTGGAGCTCGAGTGATGTCCCTAACTGATGGTCTTTCCAAGATGTCCAAATCTGCACCTTCTGACCAGTCCCGAATCAATCTTCTGGACCCAAAAGATGTCATATCAAACAAAATAAAGAGGTGCAAGACTGACTCATTTTCAGGTATGGAATTTGATAATCCTGAAAGGCCTGAGTGCAACAACCTTTTGTCAATATATCAGCTCGTTTCGGAAAAGACAAAAGAGGAAGTTGCACAAGAATGCCGAGACATGAACTGGGGCACTTTCAAATCAGTCCTATCTGATGCGTTGATTGATCATCTGCATCCTATCCAGGTTCGCTACGAGGAAATCATTTCCGACTCAGCTTATTTGGATGGAATTTTGGCAGAAGGTGCTAAGACAGCTGCAGGTATAGCTGATAATACTCTCAATAATGCCTACCAGGCTATGGGATTCTTGCGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
tRNA-synt_1b

Protein Analysis

396

Amino Acids

43.54

Weight (kDa)

8.06

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1b PF00579 77 - 347 8.1e-79 tRNA synthetases class I (W and Y)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1118
AccB1I GGYRCC 1 cut(s) 287
AccB7I CCANNNNNTGG 2 cut(s) 737, 1168
AccII CGCG 2 cut(s) 143, 145
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 6 cut(s) 42, 122, 156, 195, 569, 1180
AcsI RAATTY 2 cut(s) 887, 1098
AcvI CACGTG 1 cut(s) 695
AcyI GRCGYC 2 cut(s) 288, 449
AfiI CCNNNNNNNGG 3 cut(s) 256, 737, 1168
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 4 cut(s) 117, 137, 264, 1006
AjnI CCWGG 3 cut(s) 270, 1050, 1161
AluBI AGCT 7 cut(s) 219, 413, 743, 940, 1085, 1124, 1136
AluI AGCT 7 cut(s) 219, 413, 743, 940, 1085, 1124, 1136
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 4 cut(s) 40, 200, 471, 978
Ama87I CYCGRG 1 cut(s) 744
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 1 cut(s) 905
ApeKI GCWGC 8 cut(s) 168, 171, 174, 190, 197, 410, 676, 1124
ApoI RAATTY 2 cut(s) 887, 1098
AspLEI GCGC 4 cut(s) 109, 143, 145, 290
AspS9I GGNCC 2 cut(s) 346, 823
AsuC2I CCSGG 1 cut(s) 656
AsuHPI GGTGA 2 cut(s) 22, 674
AsuII TTCGAA 1 cut(s) 88
AvaI CYCGRG 1 cut(s) 744
AvaII GGWCC 2 cut(s) 346, 823
AxyI CCTNAGG 1 cut(s) 717
BaeGI GKGCMC 2 cut(s) 481, 1001
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 745
BbrPI CACGTG 1 cut(s) 695
Bbv12I GWGCWC 1 cut(s) 745
BbvI GCAGC 8 cut(s) 155, 158, 184, 186, 202, 422, 688, 1111
BccI CCATC 3 cut(s) 610, 758, 1088
BciT130I CCWGG 3 cut(s) 272, 1052, 1163
BciVI GTATCC 2 cut(s) 397, 430
BclI TGATCA 1 cut(s) 1033
BcnI CCSGG 1 cut(s) 656
BcoDI GTCTC 4 cut(s) 40, 200, 471, 978
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 3 cut(s) 172, 311, 1125
BfoI RGCGCY 2 cut(s) 110, 291
BfuAI ACCTGC 1 cut(s) 1118
BfuI GTATCC 2 cut(s) 397, 430
BglI GCCNNNNNGGC 1 cut(s) 1163
BisI GCNGC 8 cut(s) 169, 172, 175, 191, 198, 411, 677, 1125
BlpI GCTNAGC 1 cut(s) 705
BlsI GCNGC 8 cut(s) 170, 173, 176, 192, 199, 412, 678, 1126
Bme1390I CCNGG 4 cut(s) 272, 656, 1052, 1163
Bme18I GGWCC 2 cut(s) 346, 823
BmeT110I CYCGRG 1 cut(s) 744
BmgT120I GGNCC 2 cut(s) 346, 823
BmiI GGNNCC 2 cut(s) 289, 825
BmrFI CCNGG 4 cut(s) 272, 656, 1052, 1163
BmrI ACTGGG 1 cut(s) 1003
BmsI GCATC 4 cut(s) 71, 364, 1012, 1051
BmuI ACTGGG 1 cut(s) 1003
BpmI CTGGAG 1 cut(s) 264
Bpu1102I GCTNAGC 1 cut(s) 705
Bpu14I TTCGAA 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 656
BsaAI YACGTR 1 cut(s) 695
BsaHI GRCGYC 2 cut(s) 288, 449
BsaJI CCNNGG 3 cut(s) 271, 390, 444
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 732, 762
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 737, 1168
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 5 cut(s) 305, 705, 734, 802, 998
Bse21I CCTNAGG 1 cut(s) 717
Bse3DI GCAATG 1 cut(s) 104
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 3 cut(s) 272, 1052, 1163
BseDI CCNNGG 3 cut(s) 271, 390, 444
BseGI GGATG 2 cut(s) 1042, 1099
BseLI CCNNNNNNNGG 3 cut(s) 256, 737, 1168
BseMI GCAATG 1 cut(s) 104
BseMII CTCAG 4 cut(s) 696, 708, 900, 1095
BseNI ACTGG 5 cut(s) 305, 705, 734, 802, 998
BsePI GCGCGC 1 cut(s) 141
BseRI GAGGAG 2 cut(s) 42, 244
BseSI GKGCMC 2 cut(s) 481, 1001
BseXI GCAGC 8 cut(s) 155, 158, 184, 186, 202, 422, 688, 1111
BseYI CCCAGC 1 cut(s) 215
BsgI GTGCAG 2 cut(s) 482, 774
Bsh1236I CGCG 2 cut(s) 143, 145
BshFI GGCC 1 cut(s) 907
BshNI GGYRCC 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 745
BsiHKCI CYCGRG 1 cut(s) 744
BsiSI CCGG 4 cut(s) 94, 153, 203, 656
BslFI GGGAC 4 cut(s) 587, 638, 739, 790
BslI CCNNNNNNNGG 3 cut(s) 256, 737, 1168
BsmAI GTCTC 4 cut(s) 40, 200, 471, 978
BsmBI CGTCTC 1 cut(s) 40
BsmFI GGGAC 4 cut(s) 587, 638, 739, 790
BsmI GAATGC 1 cut(s) 983
BsnI GGCC 1 cut(s) 907
BsoBI CYCGRG 1 cut(s) 744
Bsp119I TTCGAA 1 cut(s) 88
Bsp1286I GDGCHC 3 cut(s) 481, 745, 1001
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 2 cut(s) 667, 1033
Bsp1720I GCTNAGC 1 cut(s) 705
BspACI CCGC 6 cut(s) 42, 122, 156, 195, 569, 1180
BspANI GGCC 1 cut(s) 907
BspCNI CTCAG 4 cut(s) 697, 709, 901, 1094
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 2 cut(s) 143, 145
BspLI GGNNCC 2 cut(s) 289, 825
BspMAI CTGCAG 2 cut(s) 176, 1129
BspMI ACCTGC 1 cut(s) 1118
BspT104I TTCGAA 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 104
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 5 cut(s) 305, 705, 734, 802, 998
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 3 cut(s) 271, 390, 444
BssHII GCGCGC 1 cut(s) 141
BssMI GATC 2 cut(s) 667, 1033
BssNI GRCGYC 2 cut(s) 288, 449
BssT1I CCWWGG 2 cut(s) 390, 444
Bst2UI CCWGG 3 cut(s) 272, 1052, 1163
Bst4CI ACNGT 2 cut(s) 34, 369
Bst6I CTCTTC 1 cut(s) 52
BstACI GRCGYC 2 cut(s) 288, 449
BstAPI GCANNNNNTGC 1 cut(s) 1112
BstBAI YACGTR 1 cut(s) 695
BstBI TTCGAA 1 cut(s) 88
BstC8I GCNNGC 3 cut(s) 143, 154, 195
BstDEI CTNAG 5 cut(s) 705, 717, 909, 1081, 1116
BstF5I GGATG 2 cut(s) 1042, 1099
BstFNI CGCG 2 cut(s) 143, 145
BstH2I RGCGCY 2 cut(s) 110, 291
BstHHI GCGC 4 cut(s) 109, 143, 145, 290
BstKTI GATC 2 cut(s) 670, 1036
BstMAI GTCTC 4 cut(s) 40, 200, 471, 978
BstMBI GATC 2 cut(s) 667, 1033
BstMWI GCNNNNNNNGC 8 cut(s) 130, 149, 174, 190, 1112, 1121, 1133, 1163
BstNI CCWGG 3 cut(s) 272, 1052, 1163
BstNSI RCATGY 1 cut(s) 429
BstSCI CCNGG 4 cut(s) 270, 654, 1050, 1161
BstSFI CTRYAG 3 cut(s) 172, 311, 1125
BstSLI GKGCMC 2 cut(s) 481, 1001
BstUI CGCG 2 cut(s) 143, 145
BstV1I GCAGC 8 cut(s) 155, 158, 184, 186, 202, 422, 688, 1111
Bsu36I CCTNAGG 1 cut(s) 717
BsuI GTATCC 2 cut(s) 397, 430
BsuRI GGCC 1 cut(s) 907
BtsCI GGATG 2 cut(s) 1042, 1099
BveI ACCTGC 1 cut(s) 1118
Cac8I GCNNGC 3 cut(s) 143, 154, 195
CfoI GCGC 4 cut(s) 109, 143, 145, 290
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 346, 823
CseI GACGC 1 cut(s) 438
CviAII CATG 5 cut(s) 353, 426, 470, 482, 988
DdeI CTNAG 5 cut(s) 705, 717, 909, 1081, 1116
DinI GGCGCC 1 cut(s) 289
DpnI GATC 2 cut(s) 669, 1035
DpnII GATC 2 cut(s) 667, 1033
DraI TTTAAA 1 cut(s) 550
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 743
Eco130I CCWWGG 2 cut(s) 390, 444
Eco147I AGGCCT 1 cut(s) 907
Eco24I GRGCYC 1 cut(s) 745
Eco47I GGWCC 2 cut(s) 346, 823
Eco53kI GAGCTC 1 cut(s) 743
Eco72I CACGTG 1 cut(s) 695
Eco81I CCTNAGG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 744
EcoICRI GAGCTC 1 cut(s) 743
EcoRII CCWGG 3 cut(s) 270, 1050, 1161
EcoT14I CCWWGG 2 cut(s) 390, 444
EcoT38I GRGCYC 1 cut(s) 745
EgeI GGCGCC 1 cut(s) 289
EheI GGCGCC 1 cut(s) 289
ErhI CCWWGG 2 cut(s) 390, 444
Esp3I CGTCTC 1 cut(s) 40
FaeI CATG 5 cut(s) 356, 429, 473, 485, 991
FaqI GGGAC 4 cut(s) 587, 638, 739, 790
FatI CATG 5 cut(s) 352, 425, 469, 481, 987
FauI CCCGC 2 cut(s) 202, 562
FauNDI CATATG 1 cut(s) 322
FbaI TGATCA 1 cut(s) 1033
Fnu4HI GCNGC 8 cut(s) 169, 172, 175, 191, 198, 411, 677, 1125
FokI GGATG 2 cut(s) 1029, 1106
FriOI GRGCYC 1 cut(s) 745
Fsp4HI GCNGC 8 cut(s) 169, 172, 175, 191, 198, 411, 677, 1125
FspBI CTAG 1 cut(s) 11
GlaI GCGC 4 cut(s) 108, 142, 144, 289
GluI GCNGC 8 cut(s) 169, 172, 175, 191, 198, 411, 677, 1125
GsaI CCCAGC 1 cut(s) 219
GsuI CTGGAG 1 cut(s) 264
HaeII RGCGCY 2 cut(s) 110, 291
HaeIII GGCC 1 cut(s) 907
HapII CCGG 4 cut(s) 94, 153, 203, 656
HgaI GACGC 1 cut(s) 438
HhaI GCGC 4 cut(s) 109, 143, 145, 290
Hin1I GRCGYC 2 cut(s) 288, 449
Hin1II CATG 5 cut(s) 356, 429, 473, 485, 991
Hin6I GCGC 4 cut(s) 107, 141, 143, 288
HinP1I GCGC 4 cut(s) 107, 141, 143, 288
HincII GTYRAC 1 cut(s) 690
HindII GTYRAC 1 cut(s) 690
HinfI GANTC 8 cut(s) 90, 127, 246, 541, 810, 869, 1079, 1173
HpaII CCGG 4 cut(s) 94, 153, 203, 656
HphI GGTGA 2 cut(s) 22, 674
Hpy166II GTNNAC 3 cut(s) 346, 690, 698
Hpy188I TCNGA 6 cut(s) 625, 646, 799, 949, 1021, 1078
Hpy188III TCNNGA 6 cut(s) 203, 243, 716, 807, 821, 899
Hpy8I GTNNAC 3 cut(s) 346, 690, 698
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 3 cut(s) 359, 804, 1103
HpyCH4III ACNGT 2 cut(s) 34, 369
HpyCH4IV ACGT 1 cut(s) 694
HpyF10VI GCNNNNNNNGC 8 cut(s) 130, 149, 174, 190, 1112, 1121, 1133, 1163
HpyF3I CTNAG 5 cut(s) 705, 717, 909, 1081, 1116
HpySE526I ACGT 1 cut(s) 694
Hsp92I GRCGYC 2 cut(s) 288, 449
Hsp92II CATG 5 cut(s) 356, 429, 473, 485, 991
HspAI GCGC 4 cut(s) 107, 141, 143, 288
KasI GGCGCC 1 cut(s) 287
Kpn2I TCCGGA 1 cut(s) 202
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 1033
Kzo9I GATC 2 cut(s) 667, 1033
LmnI GCTCC 2 cut(s) 205, 740
Lsp1109I GCAGC 8 cut(s) 155, 158, 184, 186, 202, 422, 688, 1111
LweI GCATC 4 cut(s) 71, 364, 1012, 1051
MaeI CTAG 1 cut(s) 11
MaeII ACGT 1 cut(s) 694
MaeIII GTNAC 1 cut(s) 511
MalI GATC 2 cut(s) 669, 1035
MboI GATC 2 cut(s) 667, 1033
MboII GAAGA 4 cut(s) 39, 57, 677, 809
MhlI GDGCHC 3 cut(s) 481, 745, 1001
MluCI AATT 4 cut(s) 117, 277, 887, 1098
Mly113I GGCGCC 1 cut(s) 288
MlyI GAGTC 3 cut(s) 255, 863, 1073
MmeI TCCRAC 3 cut(s) 274, 718, 1101
MreI CGCCGGCG 1 cut(s) 152
MroI TCCGGA 1 cut(s) 202
MroNI GCCGGC 1 cut(s) 152
MseI TTAA 2 cut(s) 503, 549
MslI CAYNNNNRTG 2 cut(s) 474, 976
MspA1I CMGCKG 2 cut(s) 197, 1124
MspI CCGG 4 cut(s) 94, 153, 203, 656
MspR9I CCNGG 4 cut(s) 272, 656, 1052, 1163
Mva1269I GAATGC 1 cut(s) 983
MvaI CCWGG 3 cut(s) 272, 1052, 1163
MvnI CGCG 2 cut(s) 143, 145
MwoI GCNNNNNNNGC 8 cut(s) 130, 149, 174, 190, 1112, 1121, 1133, 1163
NaeI GCCGGC 1 cut(s) 154
NarI GGCGCC 1 cut(s) 288
NciI CCSGG 1 cut(s) 656
NdeI CATATG 1 cut(s) 322
NdeII GATC 2 cut(s) 667, 1033
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 5 cut(s) 356, 429, 473, 485, 991
NlaIV GGNNCC 2 cut(s) 289, 825
NmeAIII GCCGAG 1 cut(s) 1007
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 1 cut(s) 429
NspV TTCGAA 1 cut(s) 88
PaeR7I CTCGAG 1 cut(s) 744
PauI GCGCGC 1 cut(s) 141
PceI AGGCCT 1 cut(s) 907
PctI GAATGC 1 cut(s) 983
PdiI GCCGGC 1 cut(s) 154
PfeI GAWTC 5 cut(s) 90, 127, 541, 810, 1173
PflFI GACNNNGTC 1 cut(s) 650
PflMI CCANNNNNTGG 2 cut(s) 737, 1168
PkrI GCNGC 8 cut(s) 170, 173, 176, 192, 199, 412, 678, 1126
PleI GAGTC 3 cut(s) 254, 863, 1073
PluTI GGCGCC 1 cut(s) 291
PmaCI CACGTG 1 cut(s) 695
PmlI CACGTG 1 cut(s) 695
PpsI GAGTC 3 cut(s) 254, 863, 1073
Ppu21I YACGTR 1 cut(s) 695
Psp124BI GAGCTC 1 cut(s) 745
Psp6I CCWGG 3 cut(s) 270, 1050, 1161
PspCI CACGTG 1 cut(s) 695
PspFI CCCAGC 1 cut(s) 215
PspGI CCWGG 3 cut(s) 270, 1050, 1161
PspN4I GGNNCC 2 cut(s) 289, 825
PspPI GGNCC 2 cut(s) 346, 823
PspXI VCTCGAGB 1 cut(s) 744
PstI CTGCAG 2 cut(s) 176, 1129
PsyI GACNNNGTC 1 cut(s) 650
PteI GCGCGC 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 1124
RseI CAYNNNNRTG 2 cut(s) 474, 976
SacI GAGCTC 1 cut(s) 745
SaqAI TTAA 2 cut(s) 503, 549
SatI GCNGC 8 cut(s) 169, 172, 175, 191, 198, 411, 677, 1125
Sau3AI GATC 2 cut(s) 667, 1033
Sau96I GGNCC 2 cut(s) 346, 823
SchI GAGTC 3 cut(s) 255, 863, 1073
ScrFI CCNGG 4 cut(s) 272, 656, 1052, 1163
SduI GDGCHC 3 cut(s) 481, 745, 1001
SfaNI GCATC 4 cut(s) 71, 364, 1012, 1051
SfcI CTRYAG 3 cut(s) 172, 311, 1125
SfoI GGCGCC 1 cut(s) 289
Sfr274I CTCGAG 1 cut(s) 744
SfuI TTCGAA 1 cut(s) 88
SgrAI CRCCGGYG 1 cut(s) 152
SinI GGWCC 2 cut(s) 346, 823
SlaI CTCGAG 1 cut(s) 744
SmiMI CAYNNNNRTG 2 cut(s) 474, 976
SmlI CTYRAG 1 cut(s) 744
SmoI CTYRAG 1 cut(s) 744
Sse9I AATT 4 cut(s) 117, 277, 887, 1098
SseBI AGGCCT 1 cut(s) 907
SsiI CCGC 6 cut(s) 42, 122, 156, 195, 569, 1180
SspDI GGCGCC 1 cut(s) 287
SspMI CTAG 1 cut(s) 11
SstI GAGCTC 1 cut(s) 745
StuI AGGCCT 1 cut(s) 907
StyD4I CCNGG 4 cut(s) 270, 654, 1050, 1161
StyI CCWWGG 2 cut(s) 390, 444
TaaI ACNGT 2 cut(s) 34, 369
TaiI ACGT 1 cut(s) 697
TaqI TCGA 2 cut(s) 88, 745
TasI AATT 4 cut(s) 117, 277, 887, 1098
TfiI GAWTC 5 cut(s) 90, 127, 541, 810, 1173
Tru1I TTAA 2 cut(s) 503, 549
Tru9I TTAA 2 cut(s) 503, 549
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 8 cut(s) 168, 171, 174, 190, 197, 410, 676, 1124
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 3 cut(s) 328, 341, 1004
Tth111I GACNNNGTC 1 cut(s) 650
Van91I CCANNNNNTGG 2 cut(s) 737, 1168
VpaK11BI GGWCC 2 cut(s) 346, 823
XapI RAATTY 2 cut(s) 887, 1098
XceI RCATGY 1 cut(s) 429
XhoI CTCGAG 1 cut(s) 744
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.