pycom15g10510
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0020132
Physical Location & Seq
Forward (+)
6969682 .. 6970534
853 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 492 bp
ATGAAGGGCGAGTCCTTTGCAATGAACGGTGGGGATGGCCTCAACAGCTACAAGAAGAATTCCAAGTTACAGGCCTTGGATAATGTTGGACCCAACACCTTTGTTTGCGTCCAGACTATCATAGAAGCTGTGAAGCTCAAGTTCAAAAATCAATTGCCTGAACTCCACGTCTTCTTCAACGACCATGTTGCCAACGACTTCAACACGCTATTAAAAGCGTTCCTGATTGACAGGACTTACATGGTGGCCAGAGTTCCAGGTTCGTTCCATGGCCAGCTCTTCCCTAAGGCCTCGATGAACTTGATGCACACCGCTTTTTGCCTTCACTGGCTAAGTAGGGTTCCTGAACAAGTCACCAAAGTAGGATCTTGTACATTGAATAAAGGAAGAATAAGCTACGTGAGTAGCTCTGATGAAGTTGTGGAGGCGTTTGGTGCTCAATTTGAGAGTGACGCGGAGGGTTCTTTGCTGCAAGGTCAGCAGAGCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

164

Amino Acids

18.11

Weight (kDa)

6.95

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 29 - 153 6.9e-40 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15125 AT1G15125
malus_domestica MD15G1116500.v1.1
prunus_persica Prupe.1G321300_v2.0.a1
pyrus_communis pycom15g10500 pycom15g10510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 455
AciI CCGC 2 cut(s) 312, 455
AclWI GGATC 1 cut(s) 373
AcoI YGGCCR 2 cut(s) 246, 271
AcsI RAATTY 1 cut(s) 58
AfaI GTAC 1 cut(s) 373
AgsI TTSAA 4 cut(s) 145, 178, 202, 379
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 1 cut(s) 256
AluBI AGCT 7 cut(s) 48, 128, 136, 277, 396, 408, 486
AluI AGCT 7 cut(s) 48, 128, 136, 277, 396, 408, 486
Alw21I GWGCWC 1 cut(s) 439
AlwI GGATC 1 cut(s) 373
AoxI GGCC 5 cut(s) 37, 72, 246, 271, 288
ApeKI GCWGC 1 cut(s) 469
ApoI RAATTY 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 1 cut(s) 346
AvaII GGWCC 1 cut(s) 89
AxyI CCTNAGG 1 cut(s) 285
BalI TGGCCA 2 cut(s) 248, 273
BbsI GAAGAC 1 cut(s) 163
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 1 cut(s) 456
BccI CCATC 1 cut(s) 29
BcgI CGANNNNNNTGC 3 cut(s) 33, 170, 204
BciT130I CCWGG 1 cut(s) 258
BisI GCNGC 1 cut(s) 470
BlsI GCNGC 1 cut(s) 471
Bme1390I CCNGG 1 cut(s) 258
Bme18I GGWCC 1 cut(s) 89
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 2 cut(s) 91, 342
BmrFI CCNGG 1 cut(s) 258
BmsI GCATC 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 122
BsaAI YACGTR 1 cut(s) 400
BsaJI CCNNGG 2 cut(s) 75, 268
Bse1I ACTGG 1 cut(s) 332
Bse21I CCTNAGG 1 cut(s) 285
Bse3DI GCAATG 1 cut(s) 27
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 2 cut(s) 75, 268
BseGI GGATG 1 cut(s) 40
BseMI GCAATG 1 cut(s) 27
BseNI ACTGG 1 cut(s) 332
BseXI GCAGC 1 cut(s) 456
Bsh1236I CGCG 1 cut(s) 455
BshFI GGCC 5 cut(s) 39, 74, 248, 273, 290
BsiHKAI GWGCWC 1 cut(s) 439
BsnI GGCC 5 cut(s) 39, 74, 248, 273, 290
Bsp1286I GDGCHC 1 cut(s) 439
Bsp1407I TGTACA 1 cut(s) 371
Bsp143I GATC 1 cut(s) 365
Bsp19I CCATGG 1 cut(s) 268
BspACI CCGC 2 cut(s) 312, 455
BspANI GGCC 5 cut(s) 39, 74, 248, 273, 290
BspFNI CGCG 1 cut(s) 455
BspLI GGNNCC 2 cut(s) 91, 342
BspPI GGATC 1 cut(s) 373
BspQI GCTCTTC 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 27
BsrGI TGTACA 1 cut(s) 371
BsrI ACTGG 1 cut(s) 332
BssECI CCNNGG 2 cut(s) 75, 268
BssMI GATC 1 cut(s) 365
BssT1I CCWWGG 2 cut(s) 75, 268
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 29
Bst6I CTCTTC 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 371
BstBAI YACGTR 1 cut(s) 400
BstC8I GCNNGC 1 cut(s) 275
BstDEI CTNAG 2 cut(s) 285, 332
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 1 cut(s) 40
BstFNI CGCG 1 cut(s) 455
BstKTI GATC 1 cut(s) 368
BstMBI GATC 1 cut(s) 365
BstMWI GCNNNNNNNGC 3 cut(s) 45, 434, 478
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 1 cut(s) 256
BstUI CGCG 1 cut(s) 455
BstV1I GCAGC 1 cut(s) 456
BstV2I GAAGAC 1 cut(s) 163
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 5 cut(s) 39, 74, 248, 273, 290
BtgI CCRYGG 1 cut(s) 268
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 89
CseI GACGC 2 cut(s) 97, 461
Csp6I GTAC 1 cut(s) 372
CviAII CATG 3 cut(s) 185, 241, 269
CviQI GTAC 1 cut(s) 372
DdeI CTNAG 2 cut(s) 285, 332
DpnI GATC 1 cut(s) 367
DpnII GATC 1 cut(s) 365
EaeI YGGCCR 2 cut(s) 246, 271
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco130I CCWWGG 2 cut(s) 75, 268
Eco147I AGGCCT 2 cut(s) 74, 290
Eco47I GGWCC 1 cut(s) 89
Eco81I CCTNAGG 1 cut(s) 285
EcoRI GAATTC 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 256
EcoT14I CCWWGG 2 cut(s) 75, 268
ErhI CCWWGG 2 cut(s) 75, 268
FaeI CATG 3 cut(s) 188, 244, 272
FaiI YATR 4 cut(s) 122, 186, 242, 270
FatI CATG 3 cut(s) 184, 240, 268
Fnu4HI GCNGC 1 cut(s) 470
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 1 cut(s) 470
GluI GCNGC 1 cut(s) 470
HaeIII GGCC 5 cut(s) 39, 74, 248, 273, 290
HgaI GACGC 2 cut(s) 97, 461
Hin1II CATG 3 cut(s) 188, 244, 272
HinfI GANTC 1 cut(s) 11
HphI GGTGA 1 cut(s) 346
Hpy188I TCNGA 1 cut(s) 412
Hpy188III TCNNGA 3 cut(s) 112, 223, 344
HpyAV CCTTC 1 cut(s) 332
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4IV ACGT 2 cut(s) 168, 399
HpyCH4V TGCA 3 cut(s) 20, 307, 472
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 434, 478
HpyF3I CTNAG 2 cut(s) 285, 332
HpySE526I ACGT 2 cut(s) 168, 399
Hsp92II CATG 3 cut(s) 188, 244, 272
Kzo9I GATC 1 cut(s) 365
LguI GCTCTTC 1 cut(s) 284
Lsp1109I GCAGC 1 cut(s) 456
LweI GCATC 1 cut(s) 294
MaeII ACGT 2 cut(s) 168, 399
MaeIII GTNAC 3 cut(s) 66, 352, 449
MalI GATC 1 cut(s) 367
MboI GATC 1 cut(s) 365
MboII GAAGA 5 cut(s) 67, 163, 166, 271, 399
MfeI CAATTG 1 cut(s) 152
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 439
MlsI TGGCCA 2 cut(s) 248, 273
MluCI AATT 3 cut(s) 58, 152, 440
MluNI TGGCCA 2 cut(s) 248, 273
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 4 cut(s) 50, 301, 418, 451
Mox20I TGGCCA 2 cut(s) 248, 273
MscI TGGCCA 2 cut(s) 248, 273
MseI TTAA 1 cut(s) 212
Msp20I TGGCCA 2 cut(s) 248, 273
MspR9I CCNGG 1 cut(s) 258
MunI CAATTG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 258
MvnI CGCG 1 cut(s) 455
MwoI GCNNNNNNNGC 3 cut(s) 45, 434, 478
NcoI CCATGG 1 cut(s) 268
NdeII GATC 1 cut(s) 365
NlaIII CATG 3 cut(s) 188, 244, 272
NlaIV GGNNCC 2 cut(s) 91, 342
NmuCI GTSAC 2 cut(s) 352, 449
PceI AGGCCT 2 cut(s) 74, 290
PciSI GCTCTTC 1 cut(s) 284
PkrI GCNGC 1 cut(s) 471
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 400
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 2 cut(s) 91, 342
PspPI GGNCC 1 cut(s) 89
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 1 cut(s) 373
RsaNI GTAC 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 1 cut(s) 470
Sau3AI GATC 1 cut(s) 365
Sau96I GGNCC 1 cut(s) 89
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 1 cut(s) 294
SinI GGWCC 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 3 cut(s) 58, 152, 440
SseBI AGGCCT 2 cut(s) 74, 290
SsiI CCGC 2 cut(s) 312, 455
StuI AGGCCT 2 cut(s) 74, 290
StyD4I CCNGG 1 cut(s) 256
StyI CCWWGG 2 cut(s) 75, 268
TaaI ACNGT 1 cut(s) 29
TaiI ACGT 2 cut(s) 171, 402
TaqI TCGA 1 cut(s) 293
TasI AATT 3 cut(s) 58, 152, 440
TatI WGTACW 1 cut(s) 371
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 1 cut(s) 332
TseFI GTSAC 2 cut(s) 352, 449
TseI GCWGC 1 cut(s) 469
Tsp45I GTSAC 2 cut(s) 352, 449
TspDTI ATGAA 4 cut(s) 17, 38, 311, 429
TspRI CASTG 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 89
XapI RAATTY 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.