Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| pyrus_communis | pycom15g12130 |
| rosa_rugosa | Rorug04G0002900 |
| rosa_samantha | Rh4BG116600 Rh5CG489700 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 246 |
| AcsI | RAATTY | 2 cut(s) | 83, 616 |
| AcuI | CTGAAG | 1 cut(s) | 429 |
| AfaI | GTAC | 1 cut(s) | 594 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 650 |
| AgsI | TTSAA | 3 cut(s) | 50, 466, 485 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 540, 572 |
| AluBI | AGCT | 7 cut(s) | 161, 174, 232, 356, 380, 407, 443 |
| AluI | AGCT | 7 cut(s) | 161, 174, 232, 356, 380, 407, 443 |
| AlwI | GGATC | 1 cut(s) | 246 |
| AoxI | GGCC | 2 cut(s) | 137, 518 |
| ApeKI | GCWGC | 2 cut(s) | 232, 430 |
| ApoI | RAATTY | 2 cut(s) | 83, 616 |
| AspS9I | GGNCC | 1 cut(s) | 653 |
| AsuC2I | CCSGG | 1 cut(s) | 608 |
| AsuHPI | GGTGA | 2 cut(s) | 270, 316 |
| AvaII | GGWCC | 1 cut(s) | 653 |
| AxyI | CCTNAGG | 1 cut(s) | 645 |
| BbvI | GCAGC | 2 cut(s) | 219, 442 |
| BccI | CCATC | 2 cut(s) | 301, 605 |
| BclI | TGATCA | 2 cut(s) | 217, 532 |
| BcnI | CCSGG | 1 cut(s) | 608 |
| BfaI | CTAG | 5 cut(s) | 135, 452, 513, 522, 678 |
| BglII | AGATCT | 1 cut(s) | 509 |
| BisI | GCNGC | 2 cut(s) | 233, 431 |
| BlpI | GCTNAGC | 1 cut(s) | 170 |
| BlsI | GCNGC | 2 cut(s) | 234, 432 |
| Bme1390I | CCNGG | 1 cut(s) | 608 |
| Bme18I | GGWCC | 1 cut(s) | 653 |
| BmgT120I | GGNCC | 1 cut(s) | 653 |
| BmrFI | CCNGG | 1 cut(s) | 608 |
| BmsI | GCATC | 4 cut(s) | 193, 201, 445, 620 |
| BpmI | CTGGAG | 1 cut(s) | 567 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 170 |
| BpuEI | CTTGAG | 1 cut(s) | 247 |
| BpuMI | CCSGG | 1 cut(s) | 608 |
| Bsa29I | ATCGAT | 2 cut(s) | 43, 241 |
| BsaBI | GATNNNNATC | 1 cut(s) | 49 |
| BsaJI | CCNNGG | 1 cut(s) | 255 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 124, 154, 540, 570 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 650 |
| Bse21I | CCTNAGG | 1 cut(s) | 645 |
| Bse3DI | GCAATG | 1 cut(s) | 569 |
| Bse8I | GATNNNNATC | 1 cut(s) | 49 |
| BseCI | ATCGAT | 2 cut(s) | 43, 241 |
| BseDI | CCNNGG | 1 cut(s) | 255 |
| BseGI | GGATG | 3 cut(s) | 535, 616, 635 |
| BseJI | GATNNNNATC | 1 cut(s) | 49 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 650 |
| BseMI | GCAATG | 1 cut(s) | 569 |
| BseMII | CTCAG | 2 cut(s) | 161, 659 |
| BseXI | GCAGC | 2 cut(s) | 219, 442 |
| BshFI | GGCC | 2 cut(s) | 139, 520 |
| BshVI | ATCGAT | 2 cut(s) | 43, 241 |
| BsiSI | CCGG | 1 cut(s) | 607 |
| BslFI | GGGAC | 1 cut(s) | 86 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 650 |
| BsmFI | GGGAC | 1 cut(s) | 86 |
| BsnI | GGCC | 2 cut(s) | 139, 520 |
| Bsp143I | GATC | 4 cut(s) | 217, 238, 509, 532 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 170 |
| BspANI | GGCC | 2 cut(s) | 139, 520 |
| BspCNI | CTCAG | 2 cut(s) | 162, 658 |
| BspDI | ATCGAT | 2 cut(s) | 43, 241 |
| BspPI | GGATC | 1 cut(s) | 246 |
| BsrDI | GCAATG | 1 cut(s) | 569 |
| BssECI | CCNNGG | 1 cut(s) | 255 |
| BssMI | GATC | 4 cut(s) | 217, 238, 509, 532 |
| BssT1I | CCWWGG | 1 cut(s) | 255 |
| Bst4CI | ACNGT | 2 cut(s) | 96, 336 |
| BstC8I | GCNNGC | 4 cut(s) | 269, 428, 445, 518 |
| BstDEI | CTNAG | 7 cut(s) | 31, 170, 350, 396, 596, 645, 662 |
| BstF5I | GGATG | 3 cut(s) | 535, 616, 635 |
| BstKTI | GATC | 4 cut(s) | 220, 241, 512, 535 |
| BstMBI | GATC | 4 cut(s) | 217, 238, 509, 532 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 568 |
| BstNSI | RCATGY | 1 cut(s) | 271 |
| BstSCI | CCNGG | 1 cut(s) | 606 |
| BstV1I | GCAGC | 2 cut(s) | 219, 442 |
| BstX2I | RGATCY | 1 cut(s) | 509 |
| BstYI | RGATCY | 1 cut(s) | 509 |
| Bsu15I | ATCGAT | 2 cut(s) | 43, 241 |
| Bsu36I | CCTNAGG | 1 cut(s) | 645 |
| BsuRI | GGCC | 2 cut(s) | 139, 520 |
| BsuTUI | ATCGAT | 2 cut(s) | 43, 241 |
| BtsCI | GGATG | 3 cut(s) | 535, 616, 635 |
| Cac8I | GCNNGC | 4 cut(s) | 269, 428, 445, 518 |
| Cfr13I | GGNCC | 1 cut(s) | 653 |
| ClaI | ATCGAT | 2 cut(s) | 43, 241 |
| Csp6I | GTAC | 1 cut(s) | 593 |
| CviAII | CATG | 7 cut(s) | 54, 215, 268, 322, 373, 490, 622 |
| CviQI | GTAC | 1 cut(s) | 593 |
| DdeI | CTNAG | 7 cut(s) | 31, 170, 350, 396, 596, 645, 662 |
| DpnI | GATC | 4 cut(s) | 219, 240, 511, 534 |
| DpnII | GATC | 4 cut(s) | 217, 238, 509, 532 |
| Eco130I | CCWWGG | 1 cut(s) | 255 |
| Eco147I | AGGCCT | 2 cut(s) | 139, 520 |
| Eco47I | GGWCC | 1 cut(s) | 653 |
| Eco57I | CTGAAG | 1 cut(s) | 429 |
| Eco81I | CCTNAGG | 1 cut(s) | 645 |
| EcoT14I | CCWWGG | 1 cut(s) | 255 |
| EcoT22I | ATGCAT | 1 cut(s) | 216 |
| ErhI | CCWWGG | 1 cut(s) | 255 |
| FaeI | CATG | 7 cut(s) | 57, 218, 271, 325, 376, 493, 625 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 655, 687 |
| FaqI | GGGAC | 1 cut(s) | 86 |
| FatI | CATG | 7 cut(s) | 53, 214, 267, 321, 372, 489, 621 |
| FauNDI | CATATG | 1 cut(s) | 79 |
| FbaI | TGATCA | 2 cut(s) | 217, 532 |
| Fnu4HI | GCNGC | 2 cut(s) | 233, 431 |
| FokI | GGATG | 3 cut(s) | 542, 623, 642 |
| Fsp4HI | GCNGC | 2 cut(s) | 233, 431 |
| FspBI | CTAG | 5 cut(s) | 135, 452, 513, 522, 678 |
| GluI | GCNGC | 2 cut(s) | 233, 431 |
| GsuI | CTGGAG | 1 cut(s) | 567 |
| HaeIII | GGCC | 2 cut(s) | 139, 520 |
| HapII | CCGG | 1 cut(s) | 607 |
| Hin1II | CATG | 7 cut(s) | 57, 218, 271, 325, 376, 493, 625 |
| HincII | GTYRAC | 1 cut(s) | 735 |
| HindII | GTYRAC | 1 cut(s) | 735 |
| HindIII | AAGCTT | 1 cut(s) | 441 |
| HinfI | GANTC | 4 cut(s) | 50, 122, 129, 260 |
| HpaI | GTTAAC | 1 cut(s) | 735 |
| HpaII | CCGG | 1 cut(s) | 607 |
| HphI | GGTGA | 2 cut(s) | 270, 316 |
| Hpy166II | GTNNAC | 1 cut(s) | 735 |
| Hpy188I | TCNGA | 1 cut(s) | 776 |
| Hpy188III | TCNNGA | 3 cut(s) | 119, 126, 546 |
| Hpy8I | GTNNAC | 1 cut(s) | 735 |
| HpyAV | CCTTC | 1 cut(s) | 383 |
| HpyCH4III | ACNGT | 2 cut(s) | 96, 336 |
| HpyCH4IV | ACGT | 1 cut(s) | 36 |
| HpyCH4V | TGCA | 6 cut(s) | 206, 214, 251, 430, 458, 714 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 568 |
| HpyF3I | CTNAG | 7 cut(s) | 31, 170, 350, 396, 596, 645, 662 |
| HpySE526I | ACGT | 1 cut(s) | 36 |
| Hsp92II | CATG | 7 cut(s) | 57, 218, 271, 325, 376, 493, 625 |
| Ksp22I | TGATCA | 2 cut(s) | 217, 532 |
| KspAI | GTTAAC | 1 cut(s) | 735 |
| Kzo9I | GATC | 4 cut(s) | 217, 238, 509, 532 |
| LmnI | GCTCC | 1 cut(s) | 364 |
| LpnPI | CCDG | 6 cut(s) | 111, 221, 502, 531, 620, 632 |
| Lsp1109I | GCAGC | 2 cut(s) | 219, 442 |
| LweI | GCATC | 4 cut(s) | 193, 201, 445, 620 |
| MaeI | CTAG | 5 cut(s) | 135, 452, 513, 522, 678 |
| MaeII | ACGT | 1 cut(s) | 36 |
| MaeIII | GTNAC | 2 cut(s) | 130, 368 |
| MalI | GATC | 4 cut(s) | 219, 240, 511, 534 |
| MboI | GATC | 4 cut(s) | 217, 238, 509, 532 |
| MboII | GAAGA | 1 cut(s) | 754 |
| MflI | RGATCY | 1 cut(s) | 509 |
| MluCI | AATT | 5 cut(s) | 25, 83, 616, 685, 727 |
| MlyI | GAGTC | 2 cut(s) | 138, 254 |
| MmeI | TCCRAC | 1 cut(s) | 607 |
| MnlI | CCTC | 4 cut(s) | 53, 150, 181, 654 |
| Mph1103I | ATGCAT | 1 cut(s) | 216 |
| MseI | TTAA | 4 cut(s) | 113, 152, 723, 734 |
| MspI | CCGG | 1 cut(s) | 607 |
| MspR9I | CCNGG | 1 cut(s) | 608 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 568 |
| NciI | CCSGG | 1 cut(s) | 608 |
| NdeI | CATATG | 1 cut(s) | 79 |
| NdeII | GATC | 4 cut(s) | 217, 238, 509, 532 |
| NlaIII | CATG | 7 cut(s) | 57, 218, 271, 325, 376, 493, 625 |
| NmuCI | GTSAC | 2 cut(s) | 130, 368 |
| NsiI | ATGCAT | 1 cut(s) | 216 |
| NspI | RCATGY | 1 cut(s) | 271 |
| PaeI | GCATGC | 1 cut(s) | 271 |
| PceI | AGGCCT | 2 cut(s) | 139, 520 |
| PfeI | GAWTC | 2 cut(s) | 50, 122 |
| PkrI | GCNGC | 2 cut(s) | 234, 432 |
| PleI | GAGTC | 2 cut(s) | 137, 254 |
| PpsI | GAGTC | 2 cut(s) | 137, 254 |
| PspPI | GGNCC | 1 cut(s) | 653 |
| PsuI | RGATCY | 1 cut(s) | 509 |
| RsaI | GTAC | 1 cut(s) | 594 |
| RsaNI | GTAC | 1 cut(s) | 593 |
| SaqAI | TTAA | 4 cut(s) | 113, 152, 723, 734 |
| SatI | GCNGC | 2 cut(s) | 233, 431 |
| Sau3AI | GATC | 4 cut(s) | 217, 238, 509, 532 |
| Sau96I | GGNCC | 1 cut(s) | 653 |
| SchI | GAGTC | 2 cut(s) | 138, 254 |
| ScrFI | CCNGG | 1 cut(s) | 608 |
| SfaNI | GCATC | 4 cut(s) | 193, 201, 445, 620 |
| SinI | GGWCC | 1 cut(s) | 653 |
| SmlI | CTYRAG | 1 cut(s) | 262 |
| SmoI | CTYRAG | 1 cut(s) | 262 |
| SphI | GCATGC | 1 cut(s) | 271 |
| Sse9I | AATT | 5 cut(s) | 25, 83, 616, 685, 727 |
| SseBI | AGGCCT | 2 cut(s) | 139, 520 |
| SspI | AATATT | 1 cut(s) | 768 |
| SspMI | CTAG | 5 cut(s) | 135, 452, 513, 522, 678 |
| StuI | AGGCCT | 2 cut(s) | 139, 520 |
| StyD4I | CCNGG | 1 cut(s) | 606 |
| StyI | CCWWGG | 1 cut(s) | 255 |
| TaaI | ACNGT | 2 cut(s) | 96, 336 |
| TaiI | ACGT | 1 cut(s) | 39 |
| TaqI | TCGA | 2 cut(s) | 43, 241 |
| TasI | AATT | 5 cut(s) | 25, 83, 616, 685, 727 |
| TfiI | GAWTC | 2 cut(s) | 50, 122 |
| Tru1I | TTAA | 4 cut(s) | 113, 152, 723, 734 |
| Tru9I | TTAA | 4 cut(s) | 113, 152, 723, 734 |
| TseFI | GTSAC | 2 cut(s) | 130, 368 |
| TseI | GCWGC | 2 cut(s) | 232, 430 |
| Tsp45I | GTSAC | 2 cut(s) | 130, 368 |
| TspDTI | ATGAA | 4 cut(s) | 96, 389, 452, 610 |
| VpaK11BI | GGWCC | 1 cut(s) | 653 |
| XapI | RAATTY | 2 cut(s) | 83, 616 |
| XceI | RCATGY | 1 cut(s) | 271 |
| XspI | CTAG | 5 cut(s) | 135, 452, 513, 522, 678 |
| Zsp2I | ATGCAT | 1 cut(s) | 216 |