Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| pyrus_communis | pycom15g12130 |
| rosa_rugosa | Rorug04G0002900 |
| rosa_samantha | Rh4BG116600 Rh5CG489700 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 210 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 209 |
| AgsI | TTSAA | 2 cut(s) | 112, 140 |
| AjnI | CCWGG | 1 cut(s) | 202 |
| AluBI | AGCT | 2 cut(s) | 50, 92 |
| AluI | AGCT | 2 cut(s) | 50, 92 |
| Alw26I | GTCTC | 1 cut(s) | 6 |
| AsuHPI | GGTGA | 1 cut(s) | 98 |
| BciT130I | CCWGG | 1 cut(s) | 204 |
| BcoDI | GTCTC | 1 cut(s) | 6 |
| Bme1390I | CCNGG | 1 cut(s) | 204 |
| BmrFI | CCNGG | 1 cut(s) | 204 |
| BmsI | GCATC | 1 cut(s) | 177 |
| BpmI | CTGGAG | 1 cut(s) | 193 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 209 |
| BseBI | CCWGG | 1 cut(s) | 204 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 209 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 209 |
| BsmAI | GTCTC | 1 cut(s) | 6 |
| BspACI | CCGC | 1 cut(s) | 210 |
| Bst2UI | CCWGG | 1 cut(s) | 204 |
| Bst4CI | ACNGT | 1 cut(s) | 163 |
| BstDEI | CTNAG | 2 cut(s) | 44, 222 |
| BstMAI | GTCTC | 1 cut(s) | 6 |
| BstNI | CCWGG | 1 cut(s) | 204 |
| BstSCI | CCNGG | 1 cut(s) | 202 |
| CviAII | CATG | 2 cut(s) | 67, 117 |
| CviJI | RGCY | 2 cut(s) | 50, 92 |
| CviKI_1 | RGCY | 2 cut(s) | 50, 92 |
| DdeI | CTNAG | 2 cut(s) | 44, 222 |
| EcoRII | CCWGG | 1 cut(s) | 202 |
| EcoT22I | ATGCAT | 1 cut(s) | 170 |
| FaeI | CATG | 2 cut(s) | 70, 120 |
| FaiI | YATR | 6 cut(s) | 24, 56, 68, 118, 166, 216 |
| FatI | CATG | 2 cut(s) | 66, 116 |
| GsuI | CTGGAG | 1 cut(s) | 193 |
| Hin1II | CATG | 2 cut(s) | 70, 120 |
| HinfI | GANTC | 2 cut(s) | 34, 196 |
| HphI | GGTGA | 1 cut(s) | 98 |
| Hpy166II | GTNNAC | 1 cut(s) | 195 |
| Hpy188III | TCNNGA | 2 cut(s) | 31, 172 |
| Hpy8I | GTNNAC | 1 cut(s) | 195 |
| Hpy99I | CGWCG | 1 cut(s) | 190 |
| HpyAV | CCTTC | 1 cut(s) | 77 |
| HpyCH4III | ACNGT | 1 cut(s) | 163 |
| HpyCH4V | TGCA | 1 cut(s) | 168 |
| HpyF3I | CTNAG | 2 cut(s) | 44, 222 |
| Hsp92II | CATG | 2 cut(s) | 70, 120 |
| LpnPI | CCDG | 4 cut(s) | 16, 157, 189, 216 |
| LweI | GCATC | 1 cut(s) | 177 |
| MaeIII | GTNAC | 1 cut(s) | 62 |
| MlyI | GAGTC | 1 cut(s) | 190 |
| Mph1103I | ATGCAT | 1 cut(s) | 170 |
| MslI | CAYNNNNRTG | 1 cut(s) | 121 |
| MspR9I | CCNGG | 1 cut(s) | 204 |
| MvaI | CCWGG | 1 cut(s) | 204 |
| NlaIII | CATG | 2 cut(s) | 70, 120 |
| NmuCI | GTSAC | 1 cut(s) | 62 |
| NsiI | ATGCAT | 1 cut(s) | 170 |
| PfeI | GAWTC | 1 cut(s) | 34 |
| PleI | GAGTC | 1 cut(s) | 190 |
| PpsI | GAGTC | 1 cut(s) | 190 |
| Psp6I | CCWGG | 1 cut(s) | 202 |
| PspGI | CCWGG | 1 cut(s) | 202 |
| RseI | CAYNNNNRTG | 1 cut(s) | 121 |
| SchI | GAGTC | 1 cut(s) | 190 |
| ScrFI | CCNGG | 1 cut(s) | 204 |
| SetI | ASST | 5 cut(s) | 45, 52, 88, 94, 150 |
| SfaNI | GCATC | 1 cut(s) | 177 |
| SgeI | CNNG | 7 cut(s) | 43, 79, 129, 161, 184, 215, 216 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 121 |
| SsiI | CCGC | 1 cut(s) | 210 |
| StyD4I | CCNGG | 1 cut(s) | 202 |
| TaaI | ACNGT | 1 cut(s) | 163 |
| TaqI | TCGA | 1 cut(s) | 185 |
| TfiI | GAWTC | 1 cut(s) | 34 |
| TseFI | GTSAC | 1 cut(s) | 62 |
| Tsp45I | GTSAC | 1 cut(s) | 62 |
| TspDTI | ATGAA | 3 cut(s) | 17, 83, 170 |
| Zsp2I | ATGCAT | 1 cut(s) | 170 |