pycom15g13250
ERF Family

Rhodanese Cell cycle control phosphatase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007175
Physical Location & Seq
Forward (+)
9076909 .. 9081683
4775 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2028 bp
ATGCTGCCTGTTTGCTCAGCCACCTCAAGCTGTTCTTCCCATTCTCAGGTAAGATGTACATGCTCGATTTCCTTACATGGAGGGTTGAATGGCAGTTCCCAGTTCCAGAAGGATTTTGAAGCCAGATACATTATGGGATGCAGGGGCTCTCTGGGCTTGTCAAAGGCAATGCCTTTTCAAAGAGATTTCTTTAAAGCACACGCTACAAAGTCGTTGTATTCGAACTTCGTGAAAAGCACAGAACAATCCGTCTCTGTGGATTTTGTTAACAGGTGCTCCCACTCATATGAATCAGACCATCTCAAGTGCAAGTATTCAGACATGTGGAGCTCTTCTACTGGAACCATGTATGAACAGCATCCGCCGGGAAGGGTGGAACTGAAGTTTGTAGACAGCTCTAGCCTATTGGCTCCTGATGAGGGACTCGTGGACTTTACTAATCCGTCAACTGAAAATGCAAGTGTCTTACCGGCAGCTGTAGAACCTGAAAGCATATCAGCTACTGATTTAACACCGGGGAATTCCATCTCTGTACCTGACTCCCTTGATGTGGATAGTGATCGATTATCCAGTGTGAAAACTAGTATTGAGGATTTTATTGATGGGGTTAGCAAGTCTTTCAGTGCTTCAGTAGATAAGGGCGAAGATATAGTTAAAACCTCAGTAGATACAATCACTTCATCAATAACATCTGTTGTTAAAAGTGCTACAGAAGCAGTGGATAATACTGTTGGCGGATTGTTTTCAACAGTGGATCAAACGGGACAATATGGTGGTAGTAAAATGACAAAATTTTCAAGCGACTTTAAGGAAGCGACAAGTAAAGGAGCTGTTGTTGCTATTGATGTCTTGAGACGTTCAATTGTTGTGGTGGAAGATTCTCTATCAAATGGGGCTTCCTTTGCTGTTAGTTCTTACCAGTCTGCCAAGGCCTTTCTCCCTCCTGACATCAGTGATGCGCTAAATTTGTCTGAAAAGAGAGTTGCAGAGTTCTTGGGGCCTGCCAAAACTGCTTTTCAACAGGTTTATATTTCGATTGAGGGGTTGGAGGAAAATCTTGGCTTGGACCCAAATGATCCGGTCATTCCATTTGTTCTATTTCTTGGCACCTCGGCCACTTTATGGGTCATTTATCGGGTGTGGACATACAGTGGTTACGCTGGAGATTTATCTCCTCAATCGACATTGGAGCTCTTGACAGGGAGAGAGAATGCTGTGCTCATTGATGTCCGACCTGAGGTTCTGAGAGAAAGAGAGGGTATTCCTGATCTTCGACGAGGAGCTCGCTTTCGCTATGCAAGTGTGTCCCTACCTGAGGTTGATGGTGCTGTATGGAAATTAGTGAAGAGTGGAAGGGATCTTAATGACACCTTAACTGCTGCTGTTATTAGGAACTTGAAGGTTGTTCAGGACAGGTCCAAGGTCATAGTTATGGATGCTGATGGTACTCGTTCCAAAGGTATTGCAAGATCCTTGAGAAAGCTTGGGCTCAAGAAACCGTACTTGGTTCAAGGTGGCTTTAAGTCTTGGGTGAAAAATGGTCTCCGTATTAAGGAACTCAAACCTGAGACAACATTTACCATACTCAATGAGGAAGCTGAGGCAATTCTGGAGGATATCAGTCCTTCTCCGGTGCAAGTTCTTGGGTCTGGTGTGGGATTGATTGCAGCATTGTATGCACTACTAGAGTGGGAGAAGACATTACAACTGATTGGCGTTGTTGGTGTTGGTCAGACCATTTATCGGCGGGTGGCATCTTATGAGACCGCAGATGATTTTAAGAAAGATGTGAGGCTGCTGCTTTCTCCTGTTAGACTGGGAGCTCAGGCACTATCATGGACTGCCGGAAAATTGGAATCAAACGGCATTGGACTGCCAACATCTCCTTCATCCTCAGATGTCAAAAACCGAGTATTGCAGGCTGCTGCAAAGCATGAATCTCAACCGTCTGATACAGAAGGCATCCAAGATCCATCTCCTGACTCAAAACTTCCAATCAAAGAGAATGCTGATCTGTCAGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

676

Amino Acids

72.88

Weight (kDa)

5.3

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 397 - 512 1.7e-06 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1106
AccB7I CCANNNNNTGG 1 cut(s) 1122
AccI GTMKAC 1 cut(s) 390
AciI CCGC 4 cut(s) 362, 735, 1747, 1767
AclWI GGATC 5 cut(s) 762, 1070, 1365, 1464, 1964
AcoI YGGCCR 1 cut(s) 1113
AcsI RAATTY 3 cut(s) 520, 791, 964
AcuI CTGAAG 2 cut(s) 401, 612
AfaI GTAC 4 cut(s) 58, 534, 1447, 1502
AfiI CCNNNNNNNGG 8 cut(s) 46, 419, 550, 1122, 1237, 1315, 1552, 1743
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 9 cut(s) 88, 119, 179, 747, 798, 861, 1019, 1399, 1511
AhlI ACTAGT 1 cut(s) 581
AjuI GAANNNNNNNTTGG 4 cut(s) 1487, 1519, 1870, 1902
Alw21I GWGCWC 6 cut(s) 278, 332, 1194, 1221, 1285, 1825
Alw26I GTCTC 5 cut(s) 256, 847, 1547, 1562, 1757
AlwI GGATC 5 cut(s) 762, 1070, 1365, 1464, 1964
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 3 cut(s) 930, 998, 1113
ApeKI GCWGC 8 cut(s) 4, 473, 1379, 1667, 1795, 1798, 1922, 1925
ApoI RAATTY 3 cut(s) 520, 791, 964
AspLEI GCGC 1 cut(s) 961
AspS9I GGNCC 3 cut(s) 998, 1066, 1416
AsuC2I CCSGG 2 cut(s) 366, 516
AsuHPI GGTGA 1 cut(s) 1543
AsuII TTCGAA 1 cut(s) 221
AvaII GGWCC 2 cut(s) 1066, 1416
AxyI CCTNAGG 2 cut(s) 1236, 1314
BanI GGYRCC 1 cut(s) 1106
BanII GRGCYC 6 cut(s) 149, 332, 1194, 1285, 1491, 1825
BarI GAAGNNNNNNTAC 2 cut(s) 209, 241
BauI CACGAG 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 1703
Bbv12I GWGCWC 6 cut(s) 278, 332, 1194, 1221, 1285, 1825
BbvCI CCTCAGC 1 cut(s) 1599
BbvI GCAGC 7 cut(s) 485, 1366, 1679, 1782, 1785, 1909, 1912
BccI CCATC 6 cut(s) 306, 533, 596, 1316, 1436, 1981
BceAI ACGGC 1 cut(s) 1879
BcnI CCSGG 2 cut(s) 366, 516
BcoDI GTCTC 5 cut(s) 256, 847, 1547, 1562, 1757
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 3 cut(s) 399, 582, 1685
BfmI CTRYAG 2 cut(s) 477, 708
BisI GCNGC 8 cut(s) 5, 474, 1380, 1668, 1796, 1799, 1923, 1926
BlpI GCTNAGC 1 cut(s) 16
BlsI GCNGC 8 cut(s) 6, 475, 1381, 1669, 1797, 1800, 1924, 1927
Bme1390I CCNGG 2 cut(s) 366, 516
Bme18I GGWCC 2 cut(s) 1066, 1416
BmgT120I GGNCC 3 cut(s) 998, 1066, 1416
BmiI GGNNCC 5 cut(s) 343, 411, 999, 1068, 1108
BmrFI CCNGG 2 cut(s) 366, 516
BmrI ACTGGG 2 cut(s) 94, 1826
BmsI GCATC 6 cut(s) 128, 367, 946, 1426, 1763, 1971
BmuI ACTGGG 2 cut(s) 94, 1826
BpiI GAAGAC 1 cut(s) 1703
BpmI CTGGAG 2 cut(s) 1182, 1631
Bpu10I CCTNAGC 2 cut(s) 1599, 1824
Bpu1102I GCTNAGC 1 cut(s) 16
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 5 cut(s) 10, 287, 871, 1475, 1495
BpuMI CCSGG 2 cut(s) 366, 516
Bsa29I ATCGAT 1 cut(s) 562
BsaBI GATNNNNATC 1 cut(s) 558
BsaI GGTCTC 2 cut(s) 1547, 1757
BsaJI CCNNGG 4 cut(s) 515, 927, 1110, 1419
BsaWI WCCGGW 2 cut(s) 1078, 1630
BsaXI ACNNNNNCTCC 4 cut(s) 260, 290, 819, 849
Bsc4I CCNNNNNNNGG 8 cut(s) 46, 419, 550, 1122, 1237, 1315, 1552, 1743
Bse118I RCCGGY 1 cut(s) 469
Bse1I ACTGG 5 cut(s) 100, 343, 570, 919, 1821
Bse21I CCTNAGG 2 cut(s) 1236, 1314
Bse3DI GCAATG 1 cut(s) 174
Bse8I GATNNNNATC 1 cut(s) 558
BseCI ATCGAT 1 cut(s) 562
BseDI CCNNGG 4 cut(s) 515, 927, 1110, 1419
BseGI GGATG 5 cut(s) 143, 358, 1441, 1889, 1962
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 8 cut(s) 46, 419, 550, 1122, 1237, 1315, 1552, 1743
BseMI GCAATG 1 cut(s) 174
BseNI ACTGG 5 cut(s) 100, 343, 570, 919, 1821
BseRI GAGGAG 2 cut(s) 1164, 1293
BseXI GCAGC 7 cut(s) 485, 1366, 1679, 1782, 1785, 1909, 1912
BshFI GGCC 3 cut(s) 932, 1000, 1115
BshNI GGYRCC 1 cut(s) 1106
BshVI ATCGAT 1 cut(s) 562
BsiHKAI GWGCWC 6 cut(s) 278, 332, 1194, 1221, 1285, 1825
BsiSI CCGG 6 cut(s) 365, 470, 515, 1079, 1631, 1845
BslFI GGGAC 3 cut(s) 435, 777, 1291
BslI CCNNNNNNNGG 8 cut(s) 46, 419, 550, 1122, 1237, 1315, 1552, 1743
BsmAI GTCTC 5 cut(s) 256, 847, 1547, 1562, 1757
BsmBI CGTCTC 2 cut(s) 256, 847
BsmFI GGGAC 3 cut(s) 435, 777, 1291
BsmI GAATGC 2 cut(s) 1216, 2011
BsnI GGCC 3 cut(s) 932, 1000, 1115
Bso31I GGTCTC 2 cut(s) 1547, 1757
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 8 cut(s) 149, 278, 332, 1194, 1221, 1285, 1491, 1825
Bsp1407I TGTACA 1 cut(s) 56
Bsp143I GATC 8 cut(s) 559, 754, 1075, 1267, 1357, 1469, 1969, 2011
Bsp1720I GCTNAGC 1 cut(s) 16
BspACI CCGC 4 cut(s) 362, 735, 1747, 1767
BspANI GGCC 3 cut(s) 932, 1000, 1115
BspDI ATCGAT 1 cut(s) 562
BspLI GGNNCC 5 cut(s) 343, 411, 999, 1068, 1108
BspPI GGATC 5 cut(s) 762, 1070, 1365, 1464, 1964
BspQI GCTCTTC 1 cut(s) 337
BspT104I TTCGAA 1 cut(s) 221
BspT107I GGYRCC 1 cut(s) 1106
BspTNI GGTCTC 2 cut(s) 1547, 1757
BsrDI GCAATG 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 469
BsrGI TGTACA 1 cut(s) 56
BsrI ACTGG 5 cut(s) 100, 343, 570, 919, 1821
BssAI RCCGGY 1 cut(s) 469
BssECI CCNNGG 4 cut(s) 515, 927, 1110, 1419
BssMI GATC 8 cut(s) 559, 754, 1075, 1267, 1357, 1469, 1969, 2011
BssSI CACGAG 1 cut(s) 425
BssT1I CCWWGG 2 cut(s) 927, 1419
Bst2BI CACGAG 1 cut(s) 425
Bst4CI ACNGT 5 cut(s) 730, 751, 1151, 1500, 1947
Bst6I CTCTTC 2 cut(s) 337, 1340
BstAPI GCANNNNNTGC 1 cut(s) 1676
BstAUI TGTACA 1 cut(s) 56
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 3 cut(s) 1002, 1285, 1920
BstENI CCTNNNNNAGG 1 cut(s) 1313
BstF5I GGATG 5 cut(s) 143, 358, 1441, 1889, 1962
BstHHI GCGC 1 cut(s) 961
BstKTI GATC 8 cut(s) 562, 757, 1078, 1270, 1360, 1472, 1972, 2014
BstMAI GTCTC 5 cut(s) 256, 847, 1547, 1562, 1757
BstMBI GATC 8 cut(s) 559, 754, 1075, 1267, 1357, 1469, 1969, 2011
BstMWI GCNNNNNNNGC 6 cut(s) 153, 713, 836, 902, 1010, 1676
BstNSI RCATGY 2 cut(s) 63, 325
BstSCI CCNGG 2 cut(s) 364, 514
BstSFI CTRYAG 2 cut(s) 477, 708
BstV1I GCAGC 7 cut(s) 485, 1366, 1679, 1782, 1785, 1909, 1912
BstV2I GAAGAC 1 cut(s) 1703
BstX2I RGATCY 3 cut(s) 1357, 1469, 1969
BstYI RGATCY 3 cut(s) 1357, 1469, 1969
Bsu15I ATCGAT 1 cut(s) 562
Bsu36I CCTNAGG 2 cut(s) 1236, 1314
BsuRI GGCC 3 cut(s) 932, 1000, 1115
BsuTUI ATCGAT 1 cut(s) 562
BtsCI GGATG 5 cut(s) 143, 358, 1441, 1889, 1962
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 6 cut(s) 577, 628, 723, 756, 958, 1156
Cac8I GCNNGC 3 cut(s) 1002, 1285, 1920
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 1 cut(s) 961
Cfr10I RCCGGY 1 cut(s) 469
Cfr13I GGNCC 3 cut(s) 998, 1066, 1416
ClaI ATCGAT 1 cut(s) 562
Csp6I GTAC 4 cut(s) 57, 533, 1446, 1501
CspCI CAANNNNNGTGG 2 cut(s) 849, 884
CviAII CATG 6 cut(s) 60, 77, 322, 346, 1836, 1934
CviQI GTAC 4 cut(s) 57, 533, 1446, 1501
DpnI GATC 8 cut(s) 561, 756, 1077, 1269, 1359, 1471, 1971, 2013
DpnII GATC 8 cut(s) 559, 754, 1075, 1267, 1357, 1469, 1969, 2011
DraI TTTAAA 1 cut(s) 193
EaeI YGGCCR 1 cut(s) 1113
Eam1104I CTCTTC 2 cut(s) 337, 1340
EarI CTCTTC 2 cut(s) 337, 1340
EciI GGCGGA 2 cut(s) 351, 750
Ecl136II GAGCTC 4 cut(s) 330, 1192, 1283, 1823
Eco130I CCWWGG 2 cut(s) 927, 1419
Eco147I AGGCCT 1 cut(s) 932
Eco24I GRGCYC 6 cut(s) 149, 332, 1194, 1285, 1491, 1825
Eco31I GGTCTC 2 cut(s) 1547, 1757
Eco32I GATATC 1 cut(s) 1618
Eco47I GGWCC 2 cut(s) 1066, 1416
Eco53kI GAGCTC 4 cut(s) 330, 1192, 1283, 1823
Eco57I CTGAAG 2 cut(s) 401, 612
Eco81I CCTNAGG 2 cut(s) 1236, 1314
EcoICRI GAGCTC 4 cut(s) 330, 1192, 1283, 1823
EcoNI CCTNNNNNAGG 1 cut(s) 1313
EcoO109I RGGNCCY 1 cut(s) 998
EcoRI GAATTC 1 cut(s) 520
EcoRV GATATC 1 cut(s) 1618
EcoT14I CCWWGG 2 cut(s) 927, 1419
EcoT38I GRGCYC 6 cut(s) 149, 332, 1194, 1285, 1491, 1825
ErhI CCWWGG 2 cut(s) 927, 1419
Esp3I CGTCTC 2 cut(s) 256, 847
FaeI CATG 6 cut(s) 63, 80, 325, 349, 1839, 1937
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FaqI GGGAC 3 cut(s) 435, 777, 1291
FatI CATG 6 cut(s) 59, 76, 321, 345, 1835, 1933
FauI CCCGC 1 cut(s) 1740
FauNDI CATATG 1 cut(s) 286
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 8 cut(s) 5, 474, 1380, 1668, 1796, 1799, 1923, 1926
FokI GGATG 5 cut(s) 150, 345, 1448, 1876, 1949
FriOI GRGCYC 6 cut(s) 149, 332, 1194, 1285, 1491, 1825
Fsp4HI GCNGC 8 cut(s) 5, 474, 1380, 1668, 1796, 1799, 1923, 1926
FspBI CTAG 3 cut(s) 399, 582, 1685
GlaI GCGC 1 cut(s) 960
GluI GCNGC 8 cut(s) 5, 474, 1380, 1668, 1796, 1799, 1923, 1926
GsuI CTGGAG 2 cut(s) 1182, 1631
HaeIII GGCC 3 cut(s) 932, 1000, 1115
HapII CCGG 6 cut(s) 365, 470, 515, 1079, 1631, 1845
HhaI GCGC 1 cut(s) 961
Hin1II CATG 6 cut(s) 63, 80, 325, 349, 1839, 1937
Hin6I GCGC 1 cut(s) 959
HinP1I GCGC 1 cut(s) 959
HincII GTYRAC 2 cut(s) 268, 447
HindII GTYRAC 2 cut(s) 268, 447
HindIII AAGCTT 1 cut(s) 1481
HinfI GANTC 7 cut(s) 290, 423, 539, 878, 1856, 1937, 1982
HpaI GTTAAC 1 cut(s) 268
HpaII CCGG 6 cut(s) 365, 470, 515, 1079, 1631, 1845
HphI GGTGA 1 cut(s) 1543
Hpy166II GTNNAC 5 cut(s) 268, 391, 430, 447, 1143
Hpy188I TCNGA 9 cut(s) 295, 319, 973, 1232, 1245, 1734, 1897, 1951, 2020
Hpy8I GTNNAC 5 cut(s) 268, 391, 430, 447, 1143
Hpy99I CGWCG 1 cut(s) 1278
HpyAV CCTTC 7 cut(s) 103, 363, 1347, 1393, 1635, 1896, 1952
HpyCH4III ACNGT 5 cut(s) 730, 751, 1151, 1500, 1947
HpyCH4IV ACGT 1 cut(s) 856
HpyF10VI GCNNNNNNNGC 6 cut(s) 153, 713, 836, 902, 1010, 1676
HpySE526I ACGT 1 cut(s) 856
Hsp92II CATG 6 cut(s) 63, 80, 325, 349, 1839, 1937
HspAI GCGC 1 cut(s) 959
KspAI GTTAAC 1 cut(s) 268
Kzo9I GATC 8 cut(s) 559, 754, 1075, 1267, 1357, 1469, 1969, 2011
LguI GCTCTTC 1 cut(s) 337
LmnI GCTCC 7 cut(s) 281, 327, 415, 827, 1189, 1280, 1820
Lsp1109I GCAGC 7 cut(s) 485, 1366, 1679, 1782, 1785, 1909, 1912
LweI GCATC 6 cut(s) 128, 367, 946, 1426, 1763, 1971
MaeI CTAG 3 cut(s) 399, 582, 1685
MaeII ACGT 1 cut(s) 856
MaeIII GTNAC 1 cut(s) 1154
MalI GATC 8 cut(s) 561, 756, 1077, 1269, 1359, 1471, 1971, 2013
MboI GATC 8 cut(s) 559, 754, 1075, 1267, 1357, 1469, 1969, 2011
MboII GAAGA 7 cut(s) 27, 324, 656, 887, 1262, 1357, 1708
MfeI CAATTG 1 cut(s) 861
MflI RGATCY 3 cut(s) 1357, 1469, 1969
MhlI GDGCHC 8 cut(s) 149, 278, 332, 1194, 1221, 1285, 1491, 1825
MluCI AATT 7 cut(s) 520, 791, 861, 964, 1337, 1605, 1850
MlyI GAGTC 3 cut(s) 417, 533, 1976
MmeI TCCRAC 2 cut(s) 1026, 1255
MslI CAYNNNNRTG 3 cut(s) 285, 1430, 1834
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 6 cut(s) 365, 470, 515, 1079, 1631, 1845
MspR9I CCNGG 2 cut(s) 366, 516
MunI CAATTG 1 cut(s) 861
Mva1269I GAATGC 2 cut(s) 1216, 2011
MwoI GCNNNNNNNGC 6 cut(s) 153, 713, 836, 902, 1010, 1676
NciI CCSGG 2 cut(s) 366, 516
NdeI CATATG 1 cut(s) 286
NdeII GATC 8 cut(s) 559, 754, 1075, 1267, 1357, 1469, 1969, 2011
NlaIII CATG 6 cut(s) 63, 80, 325, 349, 1839, 1937
NlaIV GGNNCC 5 cut(s) 343, 411, 999, 1068, 1108
NmeAIII GCCGAG 1 cut(s) 1091
NspI RCATGY 2 cut(s) 63, 325
NspV TTCGAA 1 cut(s) 221
PceI AGGCCT 1 cut(s) 932
PciI ACATGT 1 cut(s) 321
PciSI GCTCTTC 1 cut(s) 337
PcsI WCGNNNNNNNCGW 1 cut(s) 218
PctI GAATGC 2 cut(s) 1216, 2011
PfeI GAWTC 4 cut(s) 290, 878, 1856, 1937
PflMI CCANNNNNTGG 1 cut(s) 1122
PkrI GCNGC 8 cut(s) 6, 475, 1381, 1669, 1797, 1800, 1924, 1927
PleI GAGTC 3 cut(s) 417, 533, 1976
PpsI GAGTC 3 cut(s) 417, 533, 1976
PscI ACATGT 1 cut(s) 321
Psp124BI GAGCTC 4 cut(s) 332, 1194, 1285, 1825
PspN4I GGNNCC 5 cut(s) 343, 411, 999, 1068, 1108
PspPI GGNCC 3 cut(s) 998, 1066, 1416
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 3 cut(s) 1357, 1469, 1969
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 4 cut(s) 58, 534, 1447, 1502
RsaNI GTAC 4 cut(s) 57, 533, 1446, 1501
RseI CAYNNNNRTG 3 cut(s) 285, 1430, 1834
SacI GAGCTC 4 cut(s) 332, 1194, 1285, 1825
SapI GCTCTTC 1 cut(s) 337
SatI GCNGC 8 cut(s) 5, 474, 1380, 1668, 1796, 1799, 1923, 1926
Sau3AI GATC 8 cut(s) 559, 754, 1075, 1267, 1357, 1469, 1969, 2011
Sau96I GGNCC 3 cut(s) 998, 1066, 1416
SchI GAGTC 3 cut(s) 417, 533, 1976
ScrFI CCNGG 2 cut(s) 366, 516
SduI GDGCHC 8 cut(s) 149, 278, 332, 1194, 1221, 1285, 1491, 1825
SfaNI GCATC 6 cut(s) 128, 367, 946, 1426, 1763, 1971
SfcI CTRYAG 2 cut(s) 477, 708
SfuI TTCGAA 1 cut(s) 221
SinI GGWCC 2 cut(s) 1066, 1416
SmiMI CAYNNNNRTG 3 cut(s) 285, 1430, 1834
SmlI CTYRAG 5 cut(s) 25, 302, 850, 1474, 1490
SmoI CTYRAG 5 cut(s) 25, 302, 850, 1474, 1490
SpeI ACTAGT 1 cut(s) 581
Sse9I AATT 7 cut(s) 520, 791, 861, 964, 1337, 1605, 1850
SseBI AGGCCT 1 cut(s) 932
SsiI CCGC 4 cut(s) 362, 735, 1747, 1767
SspMI CTAG 3 cut(s) 399, 582, 1685
SstI GAGCTC 4 cut(s) 332, 1194, 1285, 1825
StuI AGGCCT 1 cut(s) 932
StyD4I CCNGG 2 cut(s) 364, 514
StyI CCWWGG 2 cut(s) 927, 1419
TaaI ACNGT 5 cut(s) 730, 751, 1151, 1500, 1947
TaiI ACGT 1 cut(s) 859
TaqI TCGA 6 cut(s) 65, 221, 562, 1034, 1181, 1273
TasI AATT 7 cut(s) 520, 791, 861, 964, 1337, 1605, 1850
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 4 cut(s) 290, 878, 1856, 1937
TscAI CASTG 6 cut(s) 577, 628, 723, 756, 958, 1156
TseI GCWGC 8 cut(s) 4, 473, 1379, 1667, 1795, 1798, 1922, 1925
TspDTI ATGAA 5 cut(s) 303, 366, 669, 1878, 1950
TspGWI ACGGA 3 cut(s) 238, 432, 1535
TspRI CASTG 6 cut(s) 577, 628, 723, 756, 958, 1156
Van91I CCANNNNNTGG 1 cut(s) 1122
VpaK11BI GGWCC 2 cut(s) 1066, 1416
XagI CCTNNNNNAGG 1 cut(s) 1313
XapI RAATTY 3 cut(s) 520, 791, 964
XceI RCATGY 2 cut(s) 63, 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 390
XspI CTAG 3 cut(s) 399, 582, 1685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.