pycom15g13490

eukaryotic translation initiation factor isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0020266
Physical Location & Seq
Forward (+)
9178947 .. 9180161
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1125 bp
ATGGTTCGTGATGTAATTGATTTGCGTTCCAACAACTGGGTTCCCAGACGTGAAGAGATAAAAGCCAAAACCATCACTGAAATACATACAGAGGCAGAAAAAAATCTTGGATTGCGTCCAGGGACTACTGCAAGCATCCGAAGTGGTCGTAACGTTTCTGGTGCTCAAGGGAGTACCAGCCCTGGGGGATATCCCAACAACCGACCTGGTTTAGGGGGTATGATGCCTGGAATGCCTGGAATGCCCGGAACAAGGAAGATGCCTGGGATGCCTGGAATGGATAATGACAATTGGGAGGTTCAGAGGAACCGGTCAACGCCAAGGGGTGATGGGTTTGGTATGCAGCCTGGTGGTGGGCGTGGCCAGGTGCCGCTGGTTGGAAAATCAACATCTGTGAACACAAGGCTGTTACCTCAGGGTAGTGGTGGCCTTGGCAGTGGCAAACCTAGTGCCTTACTGCAGGGTAGTGGTGCCCCTCCTGCTCGTCCATCCGACCATGCTTATGGTGCAGAGCCTGTAGCTCAAGCGCCAGTGCCTGCTAGACCTGTTGTTGCTCCTTACGTAGCACCAGTTGAAAAACCACAAGCTCCAGCTCCAGCAGCGGTTCCAAAGTTGAAACGAGATGAGCTTAGGAGAAAAACAATCTCCCTTTTTGAGGAATATTTCAGTATCGGACTCTTAGATGAAGCATTACTGTGCGTGGAGGAGCTAGAGTCTCCTGCTTACCATCCCGAGGTTGTCAAGGAAGGTATCACCCTTGCACTAGAGAAGAGTCAACCATGTGTTGGACCACTCGTGAAGCTTCTTGAGTATTTGTTTTCTAAGGAGGTGCTTACGACTAGTGACATAAGGAGTGGGTGTTTACTCTATGCATCTATCCTGAACGATGTTGGCATAGACCTGCCAAAAGCGCCAACTAACTTTGGTGAAATTATAGGGAAATTAGTCTTGGTTGGAGGTTTGGATTTCAAGGTGGTGAAAGATGTCCTGAAGGGGGTGGACGACGGCTATTTCCAGAAAGCTATATTGGATGCTGCAGTGGAGGTCGTTAGATCTAGTCCGTCAGGACAAGGCCTGTTGGAATCACAAGCATCTGATATTGACGCGTGCCGTAGCCTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05416 map03013 map05416
Pfam Domains
Protein Families
MA3 MIF4G

Protein Analysis

375

Amino Acids

39.64

Weight (kDa)

7.62

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MA3 PF02847 210 - 322 2.2e-25 MA3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30680
pyrus_communis pycom15g13490
rosa_chinensis RchiOBHm_Chr5g0028791
rosa_samantha Rh1BG001500 Rh1CG009100 Rh2DG419500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 909
AccB1I GGYRCC 2 cut(s) 367, 470
AccB7I CCANNNNNTGG 2 cut(s) 36, 785
AccII CGCG 1 cut(s) 1106
AciI CCGC 2 cut(s) 371, 602
AclI AACGTT 1 cut(s) 153
AcoI YGGCCR 1 cut(s) 361
AcuI CTGAAG 1 cut(s) 1010
AfaI GTAC 1 cut(s) 175
AflIII ACRYGT 1 cut(s) 1104
AgeI ACCGGT 1 cut(s) 309
AgsI TTSAA 3 cut(s) 575, 616, 970
AhlI ACTAGT 1 cut(s) 839
AjiI CACGTC 1 cut(s) 50
AjnI CCWGG 9 cut(s) 118, 181, 205, 226, 235, 262, 271, 346, 363
AjuI GAANNNNNNNTTGG 6 cut(s) 90, 122, 932, 964, 1010, 1042
AluBI AGCT 7 cut(s) 521, 587, 593, 628, 709, 802, 1022
AluI AGCT 7 cut(s) 521, 587, 593, 628, 709, 802, 1022
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 1 cut(s) 720
AlwNI CAGNNNCTG 2 cut(s) 515, 536
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 3 cut(s) 361, 427, 1072
ApeKI GCWGC 3 cut(s) 343, 599, 1034
AsiGI ACCGGT 1 cut(s) 309
AspLEI GCGC 2 cut(s) 529, 913
AspS9I GGNCC 1 cut(s) 788
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 4 cut(s) 338, 745, 938, 988
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 1 cut(s) 788
AxyI CCTNAGG 1 cut(s) 414
BaeGI GKGCMC 1 cut(s) 475
BalI TGGCCA 1 cut(s) 363
BanI GGYRCC 2 cut(s) 367, 470
BauI CACGAG 1 cut(s) 794
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 3 cut(s) 355, 611, 1021
BccI CCATC 4 cut(s) 80, 323, 496, 735
BceAI ACGGC 2 cut(s) 1021, 1095
BciT130I CCWGG 9 cut(s) 120, 183, 207, 228, 237, 264, 273, 348, 365
BcnI CCSGG 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 720
BcuI ACTAGT 1 cut(s) 839
BfaI CTAG 7 cut(s) 447, 540, 710, 764, 840, 1056, 1123
BfmI CTRYAG 3 cut(s) 458, 516, 1035
BfoI RGCGCY 2 cut(s) 530, 914
BfuAI ACCTGC 1 cut(s) 909
BglII AGATCT 1 cut(s) 1052
BisI GCNGC 4 cut(s) 344, 371, 600, 1035
BlsI GCNGC 4 cut(s) 345, 372, 601, 1036
Bme18I GGWCC 1 cut(s) 788
BmeT110I CYCGRG 1 cut(s) 731
BmgBI CACGTC 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 788
BmiI GGNNCC 5 cut(s) 42, 308, 369, 472, 606
BmrI ACTGGG 1 cut(s) 46
BmsI GCATC 7 cut(s) 144, 213, 249, 258, 881, 1021, 1100
BmuI ACTGGG 1 cut(s) 46
BpmI CTGGAG 2 cut(s) 573, 579
Bpu10I CCTNAGC 1 cut(s) 629
BpuEI CTTGAG 3 cut(s) 150, 507, 827
BpuMI CCSGG 1 cut(s) 246
BsaAI YACGTR 1 cut(s) 562
BsaJI CCNNGG 7 cut(s) 119, 181, 182, 263, 320, 430, 732
BsaWI WCCGGW 1 cut(s) 309
BsaXI ACNNNNNCTCC 4 cut(s) 698, 728, 844, 874
Bse118I RCCGGY 1 cut(s) 309
Bse1I ACTGG 3 cut(s) 41, 530, 569
Bse21I CCTNAGG 1 cut(s) 414
BseBI CCWGG 9 cut(s) 120, 183, 207, 228, 237, 264, 273, 348, 365
BseDI CCNNGG 7 cut(s) 119, 181, 182, 263, 320, 430, 732
BseGI GGATG 5 cut(s) 135, 273, 488, 727, 1036
BseMII CTCAG 1 cut(s) 428
BseNI ACTGG 3 cut(s) 41, 530, 569
BseRI GAGGAG 1 cut(s) 719
BseSI GKGCMC 1 cut(s) 475
BseXI GCAGC 3 cut(s) 355, 611, 1021
BsgI GTGCAG 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 1106
BshFI GGCC 3 cut(s) 363, 429, 1074
BshNI GGYRCC 2 cut(s) 367, 470
BshTI ACCGGT 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 166
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 2 cut(s) 246, 310
BslFI GGGAC 1 cut(s) 136
BsmAI GTCTC 1 cut(s) 720
BsmFI GGGAC 1 cut(s) 136
BsmI GAATGC 2 cut(s) 237, 246
BsnI GGCC 3 cut(s) 363, 429, 1074
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 166, 475
Bsp143I GATC 1 cut(s) 1052
BspACI CCGC 2 cut(s) 371, 602
BspANI GGCC 3 cut(s) 363, 429, 1074
BspCNI CTCAG 1 cut(s) 427
BspFNI CGCG 1 cut(s) 1106
BspLI GGNNCC 5 cut(s) 42, 308, 369, 472, 606
BspMAI CTGCAG 2 cut(s) 462, 1039
BspMI ACCTGC 1 cut(s) 909
BspT107I GGYRCC 2 cut(s) 367, 470
BsrFI RCCGGY 1 cut(s) 309
BsrI ACTGG 3 cut(s) 41, 530, 569
BssAI RCCGGY 1 cut(s) 309
BssECI CCNNGG 7 cut(s) 119, 181, 182, 263, 320, 430, 732
BssMI GATC 1 cut(s) 1052
BssSI CACGAG 1 cut(s) 794
BssT1I CCWWGG 2 cut(s) 320, 430
Bst2BI CACGAG 1 cut(s) 794
Bst2UI CCWGG 9 cut(s) 120, 183, 207, 228, 237, 264, 273, 348, 365
Bst4CI ACNGT 1 cut(s) 696
Bst6I CTCTTC 2 cut(s) 48, 764
BstBAI YACGTR 1 cut(s) 562
BstC8I GCNNGC 3 cut(s) 133, 537, 1108
BstDEI CTNAG 4 cut(s) 414, 629, 679, 822
BstENI CCTNNNNNAGG 2 cut(s) 210, 653
BstF5I GGATG 5 cut(s) 135, 273, 488, 727, 1036
BstFNI CGCG 1 cut(s) 1106
BstH2I RGCGCY 2 cut(s) 530, 914
BstHHI GCGC 2 cut(s) 529, 913
BstKTI GATC 1 cut(s) 1055
BstMAI GTCTC 1 cut(s) 720
BstMBI GATC 1 cut(s) 1052
BstMWI GCNNNNNNNGC 7 cut(s) 232, 241, 268, 479, 506, 599, 910
BstNI CCWGG 9 cut(s) 120, 183, 207, 228, 237, 264, 273, 348, 365
BstSFI CTRYAG 3 cut(s) 458, 516, 1035
BstSLI GKGCMC 1 cut(s) 475
BstSNI TACGTA 1 cut(s) 562
BstUI CGCG 1 cut(s) 1106
BstV1I GCAGC 3 cut(s) 355, 611, 1021
BstX2I RGATCY 1 cut(s) 1052
BstXI CCANNNNNNTGG 1 cut(s) 503
BstYI RGATCY 1 cut(s) 1052
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 3 cut(s) 363, 429, 1074
BtrI CACGTC 1 cut(s) 50
BtsCI GGATG 5 cut(s) 135, 273, 488, 727, 1036
BtsI GCAGTG 2 cut(s) 442, 1044
BtsIMutI CAGTG 4 cut(s) 75, 442, 537, 1044
BveI ACCTGC 1 cut(s) 909
Cac8I GCNNGC 3 cut(s) 133, 537, 1108
CaiI CAGNNNCTG 2 cut(s) 515, 536
CfoI GCGC 2 cut(s) 529, 913
Cfr10I RCCGGY 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 788
CseI GACGC 2 cut(s) 104, 1112
CsiI ACCWGGT 1 cut(s) 205
Csp6I GTAC 1 cut(s) 174
CspAI ACCGGT 1 cut(s) 309
CviAII CATG 2 cut(s) 497, 780
CviQI GTAC 1 cut(s) 174
DdeI CTNAG 4 cut(s) 414, 629, 679, 822
DpnI GATC 1 cut(s) 1054
DpnII GATC 1 cut(s) 1052
EaeI YGGCCR 1 cut(s) 361
Eam1104I CTCTTC 2 cut(s) 48, 764
EarI CTCTTC 2 cut(s) 48, 764
Eco105I TACGTA 1 cut(s) 562
Eco130I CCWWGG 2 cut(s) 320, 430
Eco147I AGGCCT 1 cut(s) 1074
Eco32I GATATC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 788
Eco57I CTGAAG 1 cut(s) 1010
Eco81I CCTNAGG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 731
EcoNI CCTNNNNNAGG 2 cut(s) 210, 653
EcoRII CCWGG 9 cut(s) 118, 181, 205, 226, 235, 262, 271, 346, 363
EcoRV GATATC 1 cut(s) 191
EcoT14I CCWWGG 2 cut(s) 320, 430
EcoT22I ATGCAT 1 cut(s) 874
ErhI CCWWGG 2 cut(s) 320, 430
FaeI CATG 2 cut(s) 500, 783
FaqI GGGAC 1 cut(s) 136
FatI CATG 2 cut(s) 496, 779
Fnu4HI GCNGC 4 cut(s) 344, 371, 600, 1035
FokI GGATG 5 cut(s) 122, 280, 475, 714, 1043
Fsp4HI GCNGC 4 cut(s) 344, 371, 600, 1035
FspBI CTAG 7 cut(s) 447, 540, 710, 764, 840, 1056, 1123
GlaI GCGC 2 cut(s) 528, 912
GluI GCNGC 4 cut(s) 344, 371, 600, 1035
GsuI CTGGAG 2 cut(s) 573, 579
HaeII RGCGCY 2 cut(s) 530, 914
HaeIII GGCC 3 cut(s) 363, 429, 1074
HapII CCGG 2 cut(s) 246, 310
HgaI GACGC 2 cut(s) 104, 1112
HhaI GCGC 2 cut(s) 529, 913
Hin1II CATG 2 cut(s) 500, 783
Hin6I GCGC 2 cut(s) 527, 911
HinP1I GCGC 2 cut(s) 527, 911
HincII GTYRAC 2 cut(s) 315, 776
HindII GTYRAC 2 cut(s) 315, 776
HindIII AAGCTT 1 cut(s) 800
HinfI GANTC 4 cut(s) 675, 713, 772, 1082
HpaII CCGG 2 cut(s) 246, 310
HphI GGTGA 4 cut(s) 338, 745, 938, 988
Hpy166II GTNNAC 5 cut(s) 315, 397, 776, 863, 1000
Hpy188I TCNGA 5 cut(s) 140, 303, 493, 674, 1096
Hpy188III TCNNGA 8 cut(s) 8, 731, 796, 806, 880, 988, 1015, 1065
Hpy8I GTNNAC 5 cut(s) 315, 397, 776, 863, 1000
Hpy99I CGWCG 1 cut(s) 1007
HpyAV CCTTC 2 cut(s) 740, 985
HpyCH4III ACNGT 1 cut(s) 696
HpyCH4IV ACGT 3 cut(s) 49, 153, 561
HpyCH4V TGCA 7 cut(s) 131, 343, 460, 509, 761, 872, 1037
HpyF10VI GCNNNNNNNGC 7 cut(s) 232, 241, 268, 479, 506, 599, 910
HpyF3I CTNAG 4 cut(s) 414, 629, 679, 822
HpySE526I ACGT 3 cut(s) 49, 153, 561
Hsp92II CATG 2 cut(s) 500, 783
HspAI GCGC 2 cut(s) 527, 911
Kzo9I GATC 1 cut(s) 1052
LmnI GCTCC 4 cut(s) 559, 592, 598, 706
Lsp1109I GCAGC 3 cut(s) 355, 611, 1021
LweI GCATC 7 cut(s) 144, 213, 249, 258, 881, 1021, 1100
MabI ACCWGGT 1 cut(s) 205
MaeI CTAG 7 cut(s) 447, 540, 710, 764, 840, 1056, 1123
MaeII ACGT 3 cut(s) 49, 153, 561
MaeIII GTNAC 3 cut(s) 149, 408, 842
MalI GATC 1 cut(s) 1054
MboI GATC 1 cut(s) 1052
MboII GAAGA 3 cut(s) 65, 268, 781
MfeI CAATTG 1 cut(s) 289
MflI RGATCY 1 cut(s) 1052
MhlI GDGCHC 2 cut(s) 166, 475
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 4 cut(s) 15, 289, 930, 941
MluI ACGCGT 1 cut(s) 1104
MluNI TGGCCA 1 cut(s) 363
MlyI GAGTC 3 cut(s) 669, 722, 781
MmeI TCCRAC 6 cut(s) 54, 358, 516, 766, 934, 1059
Mox20I TGGCCA 1 cut(s) 363
Mph1103I ATGCAT 1 cut(s) 874
MscI TGGCCA 1 cut(s) 363
MslI CAYNNNNRTG 2 cut(s) 501, 694
Msp20I TGGCCA 1 cut(s) 363
MspA1I CMGCKG 2 cut(s) 373, 602
MspI CCGG 2 cut(s) 246, 310
MunI CAATTG 1 cut(s) 289
Mva1269I GAATGC 2 cut(s) 237, 246
MvaI CCWGG 9 cut(s) 120, 183, 207, 228, 237, 264, 273, 348, 365
MvnI CGCG 1 cut(s) 1106
MwoI GCNNNNNNNGC 7 cut(s) 232, 241, 268, 479, 506, 599, 910
NciI CCSGG 1 cut(s) 246
NdeII GATC 1 cut(s) 1052
NlaIII CATG 2 cut(s) 500, 783
NlaIV GGNNCC 5 cut(s) 42, 308, 369, 472, 606
NmuCI GTSAC 1 cut(s) 842
NsiI ATGCAT 1 cut(s) 874
PasI CCCWGGG 1 cut(s) 182
PceI AGGCCT 1 cut(s) 1074
PctI GAATGC 2 cut(s) 237, 246
PfeI GAWTC 1 cut(s) 1082
PflMI CCANNNNNTGG 2 cut(s) 36, 785
PinAI ACCGGT 1 cut(s) 309
PkrI GCNGC 4 cut(s) 345, 372, 601, 1036
PleI GAGTC 3 cut(s) 669, 721, 780
PpsI GAGTC 3 cut(s) 669, 721, 780
Ppu21I YACGTR 1 cut(s) 562
Psp1406I AACGTT 1 cut(s) 153
Psp6I CCWGG 9 cut(s) 118, 181, 205, 226, 235, 262, 271, 346, 363
PspGI CCWGG 9 cut(s) 118, 181, 205, 226, 235, 262, 271, 346, 363
PspN4I GGNNCC 5 cut(s) 42, 308, 369, 472, 606
PspPI GGNCC 1 cut(s) 788
PstI CTGCAG 2 cut(s) 462, 1039
PstNI CAGNNNCTG 2 cut(s) 515, 536
PsuI RGATCY 1 cut(s) 1052
RsaI GTAC 1 cut(s) 175
RsaNI GTAC 1 cut(s) 174
RseI CAYNNNNRTG 2 cut(s) 501, 694
SatI GCNGC 4 cut(s) 344, 371, 600, 1035
Sau3AI GATC 1 cut(s) 1052
Sau96I GGNCC 1 cut(s) 788
SchI GAGTC 3 cut(s) 669, 722, 781
SduI GDGCHC 2 cut(s) 166, 475
SexAI ACCWGGT 1 cut(s) 205
SfaNI GCATC 7 cut(s) 144, 213, 249, 258, 881, 1021, 1100
SfcI CTRYAG 3 cut(s) 458, 516, 1035
SinI GGWCC 1 cut(s) 788
SmiMI CAYNNNNRTG 2 cut(s) 501, 694
SmlI CTYRAG 3 cut(s) 165, 522, 806
SmoI CTYRAG 3 cut(s) 165, 522, 806
SnaBI TACGTA 1 cut(s) 562
SpeI ACTAGT 1 cut(s) 839
Sse9I AATT 4 cut(s) 15, 289, 930, 941
SseBI AGGCCT 1 cut(s) 1074
SsiI CCGC 2 cut(s) 371, 602
SspI AATATT 1 cut(s) 662
SspMI CTAG 7 cut(s) 447, 540, 710, 764, 840, 1056, 1123
StuI AGGCCT 1 cut(s) 1074
StyI CCWWGG 2 cut(s) 320, 430
TaaI ACNGT 1 cut(s) 696
TaiI ACGT 3 cut(s) 52, 156, 564
TasI AATT 4 cut(s) 15, 289, 930, 941
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 1 cut(s) 1082
TscAI CASTG 4 cut(s) 82, 442, 537, 1044
TseFI GTSAC 1 cut(s) 842
TseI GCWGC 3 cut(s) 343, 599, 1034
Tsp45I GTSAC 1 cut(s) 842
TspDTI ATGAA 1 cut(s) 699
TspGWI ACGGA 1 cut(s) 1050
TspRI CASTG 4 cut(s) 82, 442, 537, 1044
Van91I CCANNNNNTGG 2 cut(s) 36, 785
VpaK11BI GGWCC 1 cut(s) 788
XagI CCTNNNNNAGG 2 cut(s) 210, 653
XspI CTAG 7 cut(s) 447, 540, 710, 764, 840, 1056, 1123
Zsp2I ATGCAT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.