pycom15g34230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0020354
Physical Location & Seq
Forward (+)
33896224 .. 33900996
4773 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1785 bp
ATGTTCACTGAAGGACTTGATAGGAGCGCCCTTCGTTGGGTTAGAGAGAAGGAGGAAGTTCCATTTTCGGGTTCTAATTTGCGGCCTAGGATTGATCCGATTATGCACATTCGCAATGGTAGTGGCGGCAGGGGGTTTGGACTCCCCCCTCCGTCTAAATTTAGAAGTGGACACTTGCCCTCCAATGCCATACCGATTCGGACAATTCCAGCCGATGGGGATGAGAGTGGGTCTGCTTCTGATAATGACAGAACCACTGATTCAGAAGATGGAGTTTATGGAGGCAGGTACTCGCTGGATTCGTCACCGCAAGATGATAGGGTTCCCAGTGCTGCTGCTCATAGGTATGGGAAGCCGTCGCAAGGGCAGACCAATTATGGCAGTGATTATACTTACTCAGATGTCAGTTCCTCGATGGACACTGTTGTGGGGAGACAGAAACCTGTGGCAGAGAGATTGGTCAGGGGAGCGAGGAAGTATCCAGTTGCGCAGAATGGTTATATGGAGGATGAGTCATCTGATTCAGCTGCAAGCTCAGAGTTTTCTACTTCACGTAGGAACTTGGAAAGTGCTGCTGAAAAGAATTTGCATTCTACAAACCTGCAGAGTAAAATTTTTCCTGATGACGATGTTCCCAGTGCACCACCCTTTAGTGGTGCAACTCAGGAAATTAGACAAGATGATGAGAAAAGTCCTACTAGAGTGCATAGGACACCGCATGCTACCTCTCCATCTGAATTCAAAACAACCCCTGGTAAAAAGCAGGATGTTGTTACTGGAAATGGGAATCTTGAACAGTTTGCAAGAACTACAACTAGTTCCGAAGCTGCTGCGCCATCATGCCCAGCTCGGCTCCCAACATATTATGCAAGTGCTCTAGGGCCATGGCATGGTGTCATTGCATATGATGCATGTGTGCGTTTGTGCCTTCATGCTTGGGCAATGGAATGCATGGAAGCTCCCATGTTCTTGGAAAATGAATGTGCTCTTCTACGAGATGCATTTAGTTTACGACAAGTGCTTTTACAATCAGAGGAAGAACTGTTGGCAAAGCAGACTTCAGAGCTTGCAAGTGAGAAAGCTGCTCCAAAACCAAAGAAAGTTGTTGGCAAAATGAAAGTGCAAGTGCGCAGAATTAAAATGGGCCTGGACCCACCTACCGGGTGCAGTATTTCGTCTATAAGGCCACCAGTAATCAAACTGGAATCCATTCGTTATCATCTCTCCAGCTTCCAGTCTACACTTGCCTCTGGATGGCAAGCCCTTCGAAAGATTCGAGTTGCACCTCGGGTACCTGCAAATGGTTCTTTTTCACGTCAAAGCTTGGCATATGTGCATGCTGGTACTCAGTATATAAAACAGGTGTCTGGACTTTTGAAAACTGGTGTAACAAGTCTACGTGACAGTTCGTCCTCATATGAAGTTGTGCATGAAACGTACTCTTGTTTGTTAAGACTGAAAAGTTCAACTGAAGAAGATGCTGTCAAGATGCAACCTGGATCCGGTGAAACTCATGTCTTCTTTCCAGATAGTTTGGGAGATGATCTAATAGTTGAAGTCTTTGATTCGAAGGGAAAGCATTTTGGTAGAGTCATTGTTCAAGTGGCAACAGTTGTTGATGACCCAGCTGACAAGCAACGCTGGTTCTCTGTCTATCGTGAACCAGAACATGAACCTGTGGGAAAGATGCAGCTATCTACATATTATTCAACAAGTTCAGATGACAATCCCAAGTGTGGCTCTGTTGCAGAAACAGTTGCATATGATCGATCATCTTTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

595

Amino Acids

65.15

Weight (kDa)

6.56

Isoelectric Point (pI)

57.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020354)

Species Orthologous Gene IDs
malus_domestica MD10G1111400.v1.1
pyrus_communis pycom08g16700 pycom15g34230
rosa_multiflora Rmu_co8209566.1_g000001 Rmu_sc0006438.1_g000004
rosa_wichuraiana Rw0G003860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 489, 1130
Acc36I ACCTGC 3 cut(s) 276, 609, 1303
Acc65I GGTACC 1 cut(s) 1291
AccB1I GGYRCC 1 cut(s) 1291
AccB7I CCANNNNNTGG 2 cut(s) 215, 890
AccI GTMKAC 2 cut(s) 1238, 1396
AciI CCGC 4 cut(s) 82, 126, 308, 716
AclWI GGATC 3 cut(s) 89, 1494, 1507
AcsI RAATTY 4 cut(s) 158, 583, 612, 737
AcuI CTGAAG 3 cut(s) 30, 1044, 1491
AfaI GTAC 4 cut(s) 290, 1293, 1345, 1439
AgsI TTSAA 7 cut(s) 742, 794, 1378, 1467, 1556, 1601, 1710
AhdI GACNNNNNGTC 1 cut(s) 1408
AhlI ACTAGT 1 cut(s) 815
AjiI CACGTC 1 cut(s) 1316
AjnI CCWGG 3 cut(s) 751, 1146, 1495
AleI CACNNNNGTG 1 cut(s) 425
Alw21I GWGCWC 3 cut(s) 643, 877, 988
Alw26I GTCTC 1 cut(s) 427
Alw44I GTGCAC 1 cut(s) 639
AlwI GGATC 3 cut(s) 89, 1494, 1507
Ama87I CYCGRG 1 cut(s) 1287
AoxI GGCC 4 cut(s) 83, 881, 1144, 1184
ApaLI GTGCAC 1 cut(s) 639
ApeKI GCWGC 8 cut(s) 332, 335, 527, 572, 827, 830, 1082, 1690
ApoI RAATTY 4 cut(s) 158, 583, 612, 737
Asp700I GAANNNNTTC 1 cut(s) 1209
Asp718I GGTACC 1 cut(s) 1291
AspA2I CCTAGG 1 cut(s) 86
AspLEI GCGC 4 cut(s) 29, 490, 835, 1131
AspS9I GGNCC 3 cut(s) 881, 1144, 1150
AsuC2I CCSGG 1 cut(s) 1162
AsuHPI GGTGA 2 cut(s) 297, 1517
AsuII TTCGAA 2 cut(s) 1267, 1568
AvaI CYCGRG 1 cut(s) 1287
AvaII GGWCC 1 cut(s) 1150
AvrII CCTAGG 1 cut(s) 86
BaeGI GKGCMC 1 cut(s) 643
BamHI GGATCC 1 cut(s) 1499
BanI GGYRCC 1 cut(s) 1291
BbsI GAAGAC 1 cut(s) 1510
Bbv12I GWGCWC 3 cut(s) 643, 877, 988
BbvI GCAGC 8 cut(s) 319, 322, 514, 559, 814, 817, 1069, 1702
BccI CCATC 6 cut(s) 209, 263, 409, 739, 844, 1248
BceAI ACGGC 1 cut(s) 340
BcgI CGANNNNNNTGC 4 cut(s) 812, 846, 1247, 1281
BciT130I CCWGG 3 cut(s) 753, 1148, 1497
BciVI GTATCC 1 cut(s) 489
BcnI CCSGG 1 cut(s) 1162
BcoDI GTCTC 1 cut(s) 427
BcuI ACTAGT 1 cut(s) 815
BfaI CTAG 4 cut(s) 87, 699, 816, 878
BfmI CTRYAG 1 cut(s) 602
BfoI RGCGCY 1 cut(s) 30
BfuAI ACCTGC 3 cut(s) 276, 609, 1303
BfuI GTATCC 1 cut(s) 489
BlnI CCTAGG 1 cut(s) 86
Bme1390I CCNGG 4 cut(s) 753, 1148, 1162, 1497
Bme18I GGWCC 1 cut(s) 1150
BmeRI GACNNNNNGTC 1 cut(s) 1408
BmeT110I CYCGRG 1 cut(s) 1287
BmgBI CACGTC 1 cut(s) 1316
BmgT120I GGNCC 3 cut(s) 881, 1144, 1150
BmiI GGNNCC 5 cut(s) 324, 854, 1152, 1293, 1501
BmrFI CCNGG 4 cut(s) 753, 1148, 1162, 1497
BmrI ACTGGG 2 cut(s) 321, 630
BmsI GCATC 5 cut(s) 898, 988, 1468, 1479, 1677
BmuI ACTGGG 2 cut(s) 321, 630
BpiI GAAGAC 1 cut(s) 1510
BpmI CTGGAG 1 cut(s) 1210
Bpu14I TTCGAA 2 cut(s) 1267, 1568
BpuMI CCSGG 1 cut(s) 1162
Bsa29I ATCGAT 1 cut(s) 1768
BsaAI YACGTR 2 cut(s) 554, 1400
BsaBI GATNNNNATC 1 cut(s) 1725
BsaJI CCNNGG 4 cut(s) 86, 751, 884, 1286
BsaWI WCCGGW 1 cut(s) 1502
BsaXI ACNNNNNCTCC 4 cut(s) 164, 194, 497, 527
Bse1I ACTGG 8 cut(s) 327, 482, 636, 781, 1190, 1206, 1234, 1387
Bse3DI GCAATG 3 cut(s) 121, 897, 948
Bse8I GATNNNNATC 1 cut(s) 1725
BseBI CCWGG 3 cut(s) 753, 1148, 1497
BseCI ATCGAT 1 cut(s) 1768
BseDI CCNNGG 4 cut(s) 86, 751, 884, 1286
BseGI GGATG 4 cut(s) 226, 514, 772, 1259
BseJI GATNNNNATC 1 cut(s) 1725
BseMI GCAATG 3 cut(s) 121, 897, 948
BseMII CTCAG 4 cut(s) 411, 549, 677, 1361
BseNI ACTGG 8 cut(s) 327, 482, 636, 781, 1190, 1206, 1234, 1387
BseSI GKGCMC 1 cut(s) 643
BseXI GCAGC 8 cut(s) 319, 322, 514, 559, 814, 817, 1069, 1702
BseYI CCCAGC 2 cut(s) 844, 1624
BsgI GTGCAG 1 cut(s) 1186
BshFI GGCC 4 cut(s) 85, 883, 1146, 1186
BshNI GGYRCC 1 cut(s) 1291
BshVI ATCGAT 1 cut(s) 1768
BsiHKAI GWGCWC 3 cut(s) 643, 877, 988
BsiHKCI CYCGRG 1 cut(s) 1287
BsiSI CCGG 2 cut(s) 1161, 1503
BsmAI GTCTC 1 cut(s) 427
BsmI GAATGC 2 cut(s) 589, 953
BsnI GGCC 4 cut(s) 85, 883, 1146, 1186
BsoBI CYCGRG 1 cut(s) 1287
Bsp119I TTCGAA 2 cut(s) 1267, 1568
Bsp1286I GDGCHC 3 cut(s) 643, 877, 988
Bsp143I GATC 5 cut(s) 94, 1499, 1543, 1765, 1769
Bsp19I CCATGG 1 cut(s) 884
BspACI CCGC 4 cut(s) 82, 126, 308, 716
BspANI GGCC 4 cut(s) 85, 883, 1146, 1186
BspCNI CTCAG 4 cut(s) 410, 548, 676, 1360
BspDI ATCGAT 1 cut(s) 1768
BspLI GGNNCC 5 cut(s) 324, 854, 1152, 1293, 1501
BspMAI CTGCAG 1 cut(s) 606
BspMI ACCTGC 3 cut(s) 276, 609, 1303
BspPI GGATC 3 cut(s) 89, 1494, 1507
BspQI GCTCTTC 1 cut(s) 993
BspT104I TTCGAA 2 cut(s) 1267, 1568
BspT107I GGYRCC 1 cut(s) 1291
BsrDI GCAATG 3 cut(s) 121, 897, 948
BsrI ACTGG 8 cut(s) 327, 482, 636, 781, 1190, 1206, 1234, 1387
BssECI CCNNGG 4 cut(s) 86, 751, 884, 1286
BssMI GATC 5 cut(s) 94, 1499, 1543, 1765, 1769
BssT1I CCWWGG 2 cut(s) 86, 884
Bst2UI CCWGG 3 cut(s) 753, 1148, 1497
Bst4CI ACNGT 6 cut(s) 424, 798, 1044, 1406, 1612, 1756
Bst6I CTCTTC 1 cut(s) 993
BstAPI GCANNNNNTGC 1 cut(s) 908
BstBAI YACGTR 2 cut(s) 554, 1400
BstBI TTCGAA 2 cut(s) 1267, 1568
BstC8I GCNNGC 5 cut(s) 532, 720, 1068, 1260, 1338
BstDEI CTNAG 4 cut(s) 397, 535, 663, 1347
BstDSI CCRYGG 1 cut(s) 884
BstF5I GGATG 4 cut(s) 226, 514, 772, 1259
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 4 cut(s) 29, 490, 835, 1131
BstKTI GATC 5 cut(s) 97, 1502, 1546, 1768, 1772
BstMAI GTCTC 1 cut(s) 427
BstMBI GATC 5 cut(s) 94, 1499, 1543, 1765, 1769
BstMWI GCNNNNNNNGC 1 cut(s) 908
BstNI CCWGG 3 cut(s) 753, 1148, 1497
BstNSI RCATGY 3 cut(s) 722, 915, 1340
BstSCI CCNGG 4 cut(s) 751, 1146, 1160, 1495
BstSFI CTRYAG 1 cut(s) 602
BstSLI GKGCMC 1 cut(s) 643
BstV1I GCAGC 8 cut(s) 319, 322, 514, 559, 814, 817, 1069, 1702
BstV2I GAAGAC 1 cut(s) 1510
BstX2I RGATCY 1 cut(s) 1499
BstXI CCANNNNNNTGG 1 cut(s) 970
BstYI RGATCY 1 cut(s) 1499
Bsu15I ATCGAT 1 cut(s) 1768
BsuI GTATCC 1 cut(s) 489
BsuRI GGCC 4 cut(s) 85, 883, 1146, 1186
BsuTUI ATCGAT 1 cut(s) 1768
BtgI CCRYGG 1 cut(s) 884
BtrI CACGTC 1 cut(s) 1316
BtsCI GGATG 4 cut(s) 226, 514, 772, 1259
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 6 cut(s) 6, 255, 334, 388, 420, 643
BveI ACCTGC 3 cut(s) 276, 609, 1303
Cac8I GCNNGC 5 cut(s) 532, 720, 1068, 1260, 1338
CfoI GCGC 4 cut(s) 29, 490, 835, 1131
Cfr13I GGNCC 3 cut(s) 881, 1144, 1150
ClaI ATCGAT 1 cut(s) 1768
Csp6I GTAC 4 cut(s) 289, 1292, 1344, 1438
CspCI CAANNNNNGTGG 2 cut(s) 103, 138
CviQI GTAC 4 cut(s) 289, 1292, 1344, 1438
DdeI CTNAG 4 cut(s) 397, 535, 663, 1347
DpnI GATC 5 cut(s) 96, 1501, 1545, 1767, 1771
DpnII GATC 5 cut(s) 94, 1499, 1543, 1765, 1769
DriI GACNNNNNGTC 1 cut(s) 1408
Eam1104I CTCTTC 1 cut(s) 993
Eam1105I GACNNNNNGTC 1 cut(s) 1408
EarI CTCTTC 1 cut(s) 993
Eco130I CCWWGG 2 cut(s) 86, 884
Eco47I GGWCC 1 cut(s) 1150
Eco57I CTGAAG 3 cut(s) 30, 1044, 1491
Eco88I CYCGRG 1 cut(s) 1287
EcoRI GAATTC 1 cut(s) 737
EcoRII CCWGG 3 cut(s) 751, 1146, 1495
EcoT14I CCWWGG 2 cut(s) 86, 884
EcoT22I ATGCAT 3 cut(s) 913, 953, 1003
ErhI CCWWGG 2 cut(s) 86, 884
FauNDI CATATG 4 cut(s) 904, 1330, 1417, 1762
FblI GTMKAC 2 cut(s) 1238, 1396
FokI GGATG 4 cut(s) 233, 521, 779, 1266
FspBI CTAG 4 cut(s) 87, 699, 816, 878
FspI TGCGCA 2 cut(s) 489, 1130
GlaI GCGC 4 cut(s) 28, 489, 834, 1130
GsaI CCCAGC 2 cut(s) 848, 1628
GsuI CTGGAG 1 cut(s) 1210
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 4 cut(s) 85, 883, 1146, 1186
HapII CCGG 2 cut(s) 1161, 1503
HhaI GCGC 4 cut(s) 29, 490, 835, 1131
Hin6I GCGC 4 cut(s) 27, 488, 833, 1129
HinP1I GCGC 4 cut(s) 27, 488, 833, 1129
HindIII AAGCTT 1 cut(s) 1321
HpaII CCGG 2 cut(s) 1161, 1503
HphI GGTGA 2 cut(s) 297, 1517
Hpy166II GTNNAC 7 cut(s) 6, 170, 641, 1010, 1239, 1397, 1661
Hpy188III TCNNGA 8 cut(s) 620, 665, 791, 1251, 1368, 1486, 1526, 1658
Hpy8I GTNNAC 7 cut(s) 6, 170, 641, 1010, 1239, 1397, 1661
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 6 cut(s) 5, 41, 43, 938, 1274, 1564
HpyCH4III ACNGT 6 cut(s) 424, 798, 1044, 1406, 1612, 1756
HpyCH4IV ACGT 4 cut(s) 553, 1315, 1399, 1436
HpyF10VI GCNNNNNNNGC 1 cut(s) 908
HpyF3I CTNAG 4 cut(s) 397, 535, 663, 1347
HpySE526I ACGT 4 cut(s) 553, 1315, 1399, 1436
HspAI GCGC 4 cut(s) 27, 488, 833, 1129
KpnI GGTACC 1 cut(s) 1295
Kzo9I GATC 5 cut(s) 94, 1499, 1543, 1765, 1769
LguI GCTCTTC 1 cut(s) 993
LmnI GCTCC 5 cut(s) 24, 467, 858, 964, 1090
Lsp1109I GCAGC 8 cut(s) 319, 322, 514, 559, 814, 817, 1069, 1702
LweI GCATC 5 cut(s) 898, 988, 1468, 1479, 1677
MaeI CTAG 4 cut(s) 87, 699, 816, 878
MaeII ACGT 4 cut(s) 553, 1315, 1399, 1436
MaeIII GTNAC 4 cut(s) 303, 772, 1387, 1400
MalI GATC 5 cut(s) 96, 1501, 1545, 1767, 1771
MboI GATC 5 cut(s) 94, 1499, 1543, 1765, 1769
MboII GAAGA 6 cut(s) 278, 980, 1049, 1484, 1487, 1510
MflI RGATCY 1 cut(s) 1499
MhlI GDGCHC 3 cut(s) 643, 877, 988
MluCI AATT 9 cut(s) 76, 158, 204, 373, 583, 612, 669, 737, 1134
MlyI GAGTC 3 cut(s) 135, 521, 1599
Mph1103I ATGCAT 3 cut(s) 913, 953, 1003
MroXI GAANNNNTTC 1 cut(s) 1209
MseI TTAA 2 cut(s) 1137, 1451
MslI CAYNNNNRTG 2 cut(s) 345, 425
MspA1I CMGCKG 2 cut(s) 527, 1628
MspI CCGG 2 cut(s) 1161, 1503
MspR9I CCNGG 4 cut(s) 753, 1148, 1162, 1497
Mva1269I GAATGC 2 cut(s) 589, 953
MvaI CCWGG 3 cut(s) 753, 1148, 1497
MwoI GCNNNNNNNGC 1 cut(s) 908
NciI CCSGG 1 cut(s) 1162
NcoI CCATGG 1 cut(s) 884
NdeI CATATG 4 cut(s) 904, 1330, 1417, 1762
NdeII GATC 5 cut(s) 94, 1499, 1543, 1765, 1769
NlaIV GGNNCC 5 cut(s) 324, 854, 1152, 1293, 1501
NmeAIII GCCGAG 1 cut(s) 829
NmuCI GTSAC 2 cut(s) 303, 1400
NsbI TGCGCA 2 cut(s) 489, 1130
NsiI ATGCAT 3 cut(s) 913, 953, 1003
NspI RCATGY 3 cut(s) 722, 915, 1340
NspV TTCGAA 2 cut(s) 1267, 1568
OliI CACNNNNGTG 1 cut(s) 425
PaeI GCATGC 2 cut(s) 722, 1340
PciSI GCTCTTC 1 cut(s) 993
PcsI WCGNNNNNNNCGW 2 cut(s) 299, 1273
PctI GAATGC 2 cut(s) 589, 953
PdmI GAANNNNTTC 1 cut(s) 1209
PfeI GAWTC 8 cut(s) 196, 260, 299, 521, 787, 1205, 1273, 1565
PflMI CCANNNNNTGG 2 cut(s) 215, 890
PleI GAGTC 3 cut(s) 135, 520, 1598
PpsI GAGTC 3 cut(s) 135, 520, 1598
Ppu21I YACGTR 2 cut(s) 554, 1400
Psp6I CCWGG 3 cut(s) 751, 1146, 1495
PspFI CCCAGC 2 cut(s) 844, 1624
PspGI CCWGG 3 cut(s) 751, 1146, 1495
PspN4I GGNNCC 5 cut(s) 324, 854, 1152, 1293, 1501
PspPI GGNCC 3 cut(s) 881, 1144, 1150
PstI CTGCAG 1 cut(s) 606
PsuI RGATCY 1 cut(s) 1499
PvuII CAGCTG 2 cut(s) 527, 1628
RsaI GTAC 4 cut(s) 290, 1293, 1345, 1439
RsaNI GTAC 4 cut(s) 289, 1292, 1344, 1438
RseI CAYNNNNRTG 2 cut(s) 345, 425
SapI GCTCTTC 1 cut(s) 993
SaqAI TTAA 2 cut(s) 1137, 1451
Sau3AI GATC 5 cut(s) 94, 1499, 1543, 1765, 1769
Sau96I GGNCC 3 cut(s) 881, 1144, 1150
SchI GAGTC 3 cut(s) 135, 521, 1599
ScrFI CCNGG 4 cut(s) 753, 1148, 1162, 1497
SduI GDGCHC 3 cut(s) 643, 877, 988
SfaNI GCATC 5 cut(s) 898, 988, 1468, 1479, 1677
SfcI CTRYAG 1 cut(s) 602
SfuI TTCGAA 2 cut(s) 1267, 1568
SinI GGWCC 1 cut(s) 1150
SmiMI CAYNNNNRTG 2 cut(s) 345, 425
SpeI ACTAGT 1 cut(s) 815
SphI GCATGC 2 cut(s) 722, 1340
Sse9I AATT 9 cut(s) 76, 158, 204, 373, 583, 612, 669, 737, 1134
SsiI CCGC 4 cut(s) 82, 126, 308, 716
SspMI CTAG 4 cut(s) 87, 699, 816, 878
StyD4I CCNGG 4 cut(s) 751, 1146, 1160, 1495
StyI CCWWGG 2 cut(s) 86, 884
TaaI ACNGT 6 cut(s) 424, 798, 1044, 1406, 1612, 1756
TaiI ACGT 4 cut(s) 556, 1318, 1402, 1439
TaqI TCGA 5 cut(s) 413, 1267, 1276, 1568, 1768
TasI AATT 9 cut(s) 76, 158, 204, 373, 583, 612, 669, 737, 1134
TauI GCSGC 2 cut(s) 85, 129
TfiI GAWTC 8 cut(s) 196, 260, 299, 521, 787, 1205, 1273, 1565
Tru1I TTAA 2 cut(s) 1137, 1451
Tru9I TTAA 2 cut(s) 1137, 1451
TscAI CASTG 6 cut(s) 13, 262, 334, 388, 427, 643
TseFI GTSAC 2 cut(s) 303, 1400
TseI GCWGC 8 cut(s) 332, 335, 527, 572, 827, 830, 1082, 1690
Tsp45I GTSAC 2 cut(s) 303, 1400
TspDTI ATGAA 6 cut(s) 920, 993, 1130, 1434, 1446, 1686
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 6 cut(s) 13, 262, 334, 388, 427, 643
Van91I CCANNNNNTGG 2 cut(s) 215, 890
VneI GTGCAC 1 cut(s) 639
VpaK11BI GGWCC 1 cut(s) 1150
XapI RAATTY 4 cut(s) 158, 583, 612, 737
XceI RCATGY 3 cut(s) 722, 915, 1340
XmaJI CCTAGG 1 cut(s) 86
XmiI GTMKAC 2 cut(s) 1238, 1396
XmnI GAANNNNTTC 1 cut(s) 1209
XspI CTAG 4 cut(s) 87, 699, 816, 878
Zsp2I ATGCAT 3 cut(s) 913, 953, 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.