pycom15g39120

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0021152
Physical Location & Seq
Reverse (-)
38850318 .. 38850941
624 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 624 bp
ATGCATTTGAAGAATAACGTGATGATGGTGGTGCAGTCAGTACTACTTGTGATGATGATGTTCGGCCTCATGGCCCCCTCCCAAACCAATGCCTTGATTCCGTACAACCTAATATCCCCCTCCTCCCTCTTGGACCACATGATGATCAATGACCCCTTCAGAACTCTCGAACAGACTCCCTTCACCGTCCCAAACCCTTCTAAAGTCGTTCAAGAGGCGCTCGCTCTGGCACGTGCTGACTGGAAAGAGACGGCGACTGCACACGTGATCTCATTGGACGTCCCGGGGCTGAAGAAGGAGGATGTCAAGATAGAGGTGGAGGAGAACAGGATGCTGAGGATCAGCGGAGAGAGGAAGGCCGACGACCGGGAAGGTGAACTGCAGGGCGACAACTACAAGTGGCACCGCGCTGAGAGGACAAACGGCAAGTTCTGGAGGCAGTTCAGACTGCCTGGGAACGCCGACTTGGATCAGATAAAGGCTCACCTGGAGGACGGGGTGCTGAAGATTACGGTGCCAAAGTTAGCGGCGGAGAAGAAGAGGCAGCCCAAGCTCATCAACATCGCCTCTTCTTCCTCGTCTGCTGATCATGGGGCGGAAGTCAAACCTACAAAGGTCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

208

Amino Acids

23.23

Weight (kDa)

8.75

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 80 - 188 1.6e-29 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021152)

Species Orthologous Gene IDs
malus_domestica MD08G1249100.v1.1 MD15G1443700.v1.1
prunus_persica Prupe.1G586200_v2.0.a1
pyrus_communis pycom08g21560 pycom15g39120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 2 cut(s) 402, 514
AccII CGCG 1 cut(s) 408
AciI CCGC 5 cut(s) 345, 406, 527, 530, 596
AclWI GGATC 2 cut(s) 347, 477
AcuI CTGAAG 3 cut(s) 142, 311, 524
AcvI CACGTG 2 cut(s) 233, 265
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 2 cut(s) 42, 104
AfiI CCNNNNNNNGG 1 cut(s) 366
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 2 cut(s) 10, 212
AjnI CCWGG 2 cut(s) 451, 486
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AluBI AGCT 1 cut(s) 553
AluI AGCT 1 cut(s) 553
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 2 cut(s) 347, 477
Ama87I CYCGRG 1 cut(s) 283
AoxI GGCC 3 cut(s) 64, 72, 357
ApeKI GCWGC 1 cut(s) 544
AspLEI GCGC 2 cut(s) 220, 410
AspS9I GGNCC 2 cut(s) 73, 133
AsuC2I CCSGG 3 cut(s) 284, 285, 368
AsuHPI GGTGA 3 cut(s) 175, 386, 476
AvaI CYCGRG 1 cut(s) 283
AvaII GGWCC 1 cut(s) 133
BanI GGYRCC 2 cut(s) 402, 514
BbrPI CACGTG 2 cut(s) 233, 265
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 1 cut(s) 556
BccI CCATC 1 cut(s) 19
BceAI ACGGC 2 cut(s) 267, 439
BciT130I CCWGG 2 cut(s) 453, 488
BclI TGATCA 2 cut(s) 144, 586
BcnI CCSGG 3 cut(s) 284, 285, 368
BcoDI GTCTC 1 cut(s) 242
BfmI CTRYAG 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 221
BisI GCNGC 2 cut(s) 528, 545
BlsI GCNGC 2 cut(s) 529, 546
BmcAI AGTACT 1 cut(s) 42
Bme1390I CCNGG 5 cut(s) 284, 285, 368, 453, 488
Bme18I GGWCC 1 cut(s) 133
BmeT110I CYCGRG 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 73, 133
BmiI GGNNCC 3 cut(s) 75, 404, 516
BmrFI CCNGG 5 cut(s) 284, 285, 368, 453, 488
BmsI GCATC 1 cut(s) 321
BpmI CTGGAG 2 cut(s) 454, 509
Bpu10I CCTNAGC 1 cut(s) 335
BpuMI CCSGG 3 cut(s) 284, 285, 368
BsaAI YACGTR 2 cut(s) 233, 265
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 283, 284, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 1 cut(s) 245
BseBI CCWGG 2 cut(s) 453, 488
BseDI CCNNGG 3 cut(s) 283, 284, 452
BseGI GGATG 2 cut(s) 307, 336
BseLI CCNNNNNNNGG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 326, 402
BseNI ACTGG 1 cut(s) 245
BseRI GAGGAG 2 cut(s) 112, 335
BseXI GCAGC 1 cut(s) 556
BsgI GTGCAG 2 cut(s) 53, 243
Bsh1236I CGCG 1 cut(s) 408
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 3 cut(s) 66, 74, 359
BshNI GGYRCC 2 cut(s) 402, 514
BsiEI CGRYCG 1 cut(s) 367
BsiHKCI CYCGRG 1 cut(s) 283
BsiSI CCGG 2 cut(s) 284, 367
BslFI GGGAC 2 cut(s) 173, 266
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 1 cut(s) 242
BsmBI CGTCTC 1 cut(s) 242
BsmFI GGGAC 2 cut(s) 173, 266
BsnI GGCC 3 cut(s) 66, 74, 359
BsoBI CYCGRG 1 cut(s) 283
Bsp143I GATC 5 cut(s) 144, 267, 339, 469, 586
BspACI CCGC 5 cut(s) 345, 406, 527, 530, 596
BspANI GGCC 3 cut(s) 66, 74, 359
BspCNI CTCAG 2 cut(s) 327, 403
BspFNI CGCG 1 cut(s) 408
BspLI GGNNCC 3 cut(s) 75, 404, 516
BspMAI CTGCAG 1 cut(s) 384
BspPI GGATC 2 cut(s) 347, 477
BspT107I GGYRCC 2 cut(s) 402, 514
BsrI ACTGG 1 cut(s) 245
BssECI CCNNGG 3 cut(s) 283, 284, 452
BssMI GATC 5 cut(s) 144, 267, 339, 469, 586
BssNI GRCGYC 1 cut(s) 279
Bst2UI CCWGG 2 cut(s) 453, 488
Bst4CI ACNGT 2 cut(s) 187, 514
Bst6I CTCTTC 2 cut(s) 533, 574
BstACI GRCGYC 1 cut(s) 279
BstBAI YACGTR 2 cut(s) 233, 265
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 2 cut(s) 335, 411
BstF5I GGATG 2 cut(s) 307, 336
BstFNI CGCG 1 cut(s) 408
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 2 cut(s) 220, 410
BstKTI GATC 5 cut(s) 147, 270, 342, 472, 589
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 5 cut(s) 144, 267, 339, 469, 586
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 1 cut(s) 550
BstNI CCWGG 2 cut(s) 453, 488
BstSCI CCNGG 5 cut(s) 282, 283, 366, 451, 486
BstSFI CTRYAG 1 cut(s) 380
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 556
BsuRI GGCC 3 cut(s) 66, 74, 359
BtgZI GCGATG 1 cut(s) 547
BtsCI GGATG 2 cut(s) 307, 336
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 2 cut(s) 220, 410
Cfr13I GGNCC 2 cut(s) 73, 133
Cfr9I CCCGGG 1 cut(s) 283
Csp6I GTAC 2 cut(s) 41, 103
CviAII CATG 4 cut(s) 70, 139, 590, 621
CviJI RGCY 7 cut(s) 66, 74, 289, 359, 482, 547, 553
CviKI_1 RGCY 7 cut(s) 66, 74, 289, 359, 482, 547, 553
CviQI GTAC 2 cut(s) 41, 103
DdeI CTNAG 2 cut(s) 335, 411
DpnI GATC 5 cut(s) 146, 269, 341, 471, 588
DpnII GATC 5 cut(s) 144, 267, 339, 469, 586
Eam1104I CTCTTC 2 cut(s) 533, 574
EarI CTCTTC 2 cut(s) 533, 574
EciI GGCGGA 2 cut(s) 545, 611
Eco47I GGWCC 1 cut(s) 133
Eco57I CTGAAG 3 cut(s) 142, 311, 524
Eco72I CACGTG 2 cut(s) 233, 265
Eco88I CYCGRG 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 451, 486
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 4 cut(s) 73, 142, 593, 624
FaiI YATR 4 cut(s) 71, 140, 591, 622
FaqI GGGAC 2 cut(s) 173, 266
FatI CATG 4 cut(s) 69, 138, 589, 620
FbaI TGATCA 2 cut(s) 144, 586
Fnu4HI GCNGC 2 cut(s) 528, 545
FokI GGATG 2 cut(s) 314, 343
Fsp4HI GCNGC 2 cut(s) 528, 545
GlaI GCGC 2 cut(s) 219, 409
GluI GCNGC 2 cut(s) 528, 545
GsuI CTGGAG 2 cut(s) 454, 509
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 3 cut(s) 66, 74, 359
HapII CCGG 2 cut(s) 284, 367
HhaI GCGC 2 cut(s) 220, 410
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 4 cut(s) 73, 142, 593, 624
Hin6I GCGC 2 cut(s) 218, 408
HinP1I GCGC 2 cut(s) 218, 408
HinfI GANTC 2 cut(s) 97, 175
HpaII CCGG 2 cut(s) 284, 367
HphI GGTGA 3 cut(s) 175, 386, 476
Hpy166II GTNNAC 1 cut(s) 377
Hpy188I TCNGA 3 cut(s) 161, 446, 474
Hpy188III TCNNGA 4 cut(s) 167, 212, 307, 433
Hpy8I GTNNAC 1 cut(s) 377
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 6 cut(s) 166, 190, 207, 289, 349, 365
HpyCH4III ACNGT 2 cut(s) 187, 514
HpyCH4IV ACGT 4 cut(s) 18, 232, 264, 279
HpyCH4V TGCA 4 cut(s) 4, 34, 260, 382
HpyF10VI GCNNNNNNNGC 1 cut(s) 550
HpyF3I CTNAG 2 cut(s) 335, 411
HpySE526I ACGT 4 cut(s) 18, 232, 264, 279
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 4 cut(s) 73, 142, 593, 624
HspAI GCGC 2 cut(s) 218, 408
Ksp22I TGATCA 2 cut(s) 144, 586
Kzo9I GATC 5 cut(s) 144, 267, 339, 469, 586
Lsp1109I GCAGC 1 cut(s) 556
LweI GCATC 1 cut(s) 321
MaeII ACGT 4 cut(s) 18, 232, 264, 279
MalI GATC 5 cut(s) 146, 269, 341, 471, 588
MboI GATC 5 cut(s) 144, 267, 339, 469, 586
MboII GAAGA 7 cut(s) 22, 304, 517, 547, 550, 561, 564
MlyI GAGTC 1 cut(s) 169
Mph1103I ATGCAT 1 cut(s) 6
MspA1I CMGCKG 1 cut(s) 345
MspI CCGG 2 cut(s) 284, 367
MspR9I CCNGG 5 cut(s) 284, 285, 368, 453, 488
MvaI CCWGG 2 cut(s) 453, 488
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 1 cut(s) 550
NciI CCSGG 3 cut(s) 284, 285, 368
NdeII GATC 5 cut(s) 144, 267, 339, 469, 586
NlaIII CATG 4 cut(s) 73, 142, 593, 624
NlaIV GGNNCC 3 cut(s) 75, 404, 516
NsiI ATGCAT 1 cut(s) 6
PfeI GAWTC 1 cut(s) 97
PkrI GCNGC 2 cut(s) 529, 546
PleI GAGTC 1 cut(s) 169
PmaCI CACGTG 2 cut(s) 233, 265
PmlI CACGTG 2 cut(s) 233, 265
PpsI GAGTC 1 cut(s) 169
Ppu21I YACGTR 2 cut(s) 233, 265
Psp6I CCWGG 2 cut(s) 451, 486
PspCI CACGTG 2 cut(s) 233, 265
PspGI CCWGG 2 cut(s) 451, 486
PspN4I GGNNCC 3 cut(s) 75, 404, 516
PspPI GGNCC 2 cut(s) 73, 133
PstI CTGCAG 1 cut(s) 384
RsaI GTAC 2 cut(s) 42, 104
RsaNI GTAC 2 cut(s) 41, 103
SatI GCNGC 2 cut(s) 528, 545
Sau3AI GATC 5 cut(s) 144, 267, 339, 469, 586
Sau96I GGNCC 2 cut(s) 73, 133
ScaI AGTACT 1 cut(s) 42
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 5 cut(s) 284, 285, 368, 453, 488
SfaNI GCATC 1 cut(s) 321
SfcI CTRYAG 1 cut(s) 380
SinI GGWCC 1 cut(s) 133
SmaI CCCGGG 1 cut(s) 285
SsiI CCGC 5 cut(s) 345, 406, 527, 530, 596
StyD4I CCNGG 5 cut(s) 282, 283, 366, 451, 486
TaaI ACNGT 2 cut(s) 187, 514
TaiI ACGT 4 cut(s) 21, 235, 267, 282
TaqI TCGA 1 cut(s) 168
TatI WGTACW 1 cut(s) 40
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 1 cut(s) 97
TseI GCWGC 1 cut(s) 544
TspGWI ACGGA 1 cut(s) 90
TspMI CCCGGG 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 133
XmaI CCCGGG 1 cut(s) 283
ZraI GACGTC 1 cut(s) 280
ZrmI AGTACT 1 cut(s) 42
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.