pycom16g00040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014487
Physical Location & Seq
Forward (+)
20645 .. 26117
5473 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3156 bp
ATGCCATCCAAGACACCAACACCGTCGTTGGAGAGCAGCCACGGCGGCACCTACTTCCAAAGCCTCTTGAACTCGACCCGCCCCTTCCTCAGGGGTGAGCTGGAGTCCGTTGACAAAAATCTGCCTTCTCTGGTGGCCGTGCTGCGCTCAGTTGGAGCTGGCGAGTGCTGGCACAAGCACGGCACCTTTCTCGAACACCTCGTTGACATCTACCGCATTCTCAAGATATGGAAGGCCCCTGACTCTGTCTGCCTCTGCTCTGCGGCCTCCGTTCGTGCCCATGTCGGCGAGGCTGCGGAACGCTTGATTCACTTGTTCTGCATTGTCCCCAGACAGCCTCTCATCCACGACGACCTTTTGTTCCACTTCACCGACGCGGAGCTCGTGGAGCATCTCAAACAATCAGAGATGTCGCTGCAGCAGCTGCAGAGCCAATCGGAAGCCGCAAGCCCGAATTGTAGTTGGAGGAACAAGTTGCAGAGTCTTGTTCCTGCAAATGGGGTTACAGTAAAGCACATAAAGACCGGGGAAGATGTGTTGGTATCGAGGAGGGTGGTTGCAGTGTTTGTCCTGATGACCATGGCAGATTTCAGTGATCAACTCTTTGGTTTCCAGGACGAGCTCTTTGATAACAAAGATGGCCGCCTCGAGTTTAGGGGCAACAACTATGGAGCTCTGTGGCCCCGCGATGGCATGCCAGGACTTTGGATGAATTCCATATCAAGGATGGCTGCAATATACAATTTGATAGTGAGAGAGGAGGAGATGTTTATCCAAGAGAAGAAAACTACTAGTACTGCTACTGCTACTGCTGGAAGTGGAGGGGGTGAGAATGCCGGGGCGGACAAATGTAGAGATGAAGACATCGAGCAGGTGGTGCCACCAGTGTTTGACAAATGCACAAGGGTTCTGGATGCGGAAGAGCATTTGGCTGCAAGGGAATTGAACTGGGAAGCTGTATGTGATGTGTCTCAAAAACAGGGAAAGAAAGGAGAGGACAGAGCTGAAGAGTTGCTGTTGAGGTGTGTTGAGAAGAACCCTTTCCTTGGGGAGCCGCATGTGGTGTTGGGTCAGGTTTATTTGACAGAAGCAAGGTTTGAGGAGGCGGAGAGGGAGGCACAGAGAGGGCTAACCCGGATGCTGGAATGGGGGAGTGCTTGGGACAAGAGGATAACTTGGGAAGGATGGATTGCTTGGGCAAGGGTGCTCCTCATGAAGGCAAAGGACAGAAGCTGGCCACAAACTTCCTGGGGCATCGTCAACTTGGGCCTTGTCAGACCTCCTCCTCCTCCTCCCCCCAAAATGAGAATGCCGGTCCTCCTCCTCCTCCTCCTCACATGGAGATGGCGATGGAGATGGGTTCGTATTTCCGATTCCAACCTATCATTGCTCATGTTTTTGTCAATCCTGCTGTGTTCTACATCCTCAGTGTCGTCGTCATCTCCGGCTGTTTGCTCGGAAGCAGATAGAGCCTCCCTTCTCAGCTTCAAATCCAGAATTTTCAAGGACACAACACAAATGCTGTCTTCGTGGACGGGCAGAGACTGCTGCGCCGGAGGCTGGGAAGGTGTTGAGTGCAACCCAGGTGGTAGGGTTACTGTCTTGCAGTTGCAGCGCCCAGCTTCCGGACGTGATTACATGAAGGGGACTCTCGCCCCTTCTCTTGCCAACTTGAATTTCTTGGAGGTACTCGTTATCAGTGGGCTGAAACTAATTACAGGTCCAATTCCTCAGAGCTTCTCCAATCTCATCCACCTCACCCAACTCGCCCTCGAAGACAACTCCCTCTCTGGCCCCATTCCTTCCGGTCTAGGCCGTTTGTCCTCCCTCCAGAGCCTCTCCCTCAGCGGCAACCGTTTCAGCGGCCACATTCCCCCAAGCCTAGGCCATCTTGTCCATCTTCTCCAACTAGGTTTAGCAAGAAACTCCCTCACAGGTCCTATCCCACCCACCTTTCTAAATTTCCATGCTCTGCAGTACCTTGATTTGAGCTTCAATGCCTTGTCCGGCCCCATTCCAGATTTCGTAGGTCGCCAGCTGCAAAACCTTACTTACATTGATCTCTCCAATAACCAGTTATCTGGTCAGATGCCCATTGCCCTCTTCAGCTTGTCCAAGCTTTTGGACCTGTCCTTGAACCACAACCAACTGACTGGCATCATCCCTGTTCAGGTTGCCGGACTCAAATCTCTCACCACTCTTTCGGTGAGCGCCAATCGACTTACAGGACGTATTCCGGTATCCATTTCAAGATTAAACAACCTTTGGTACCTCAATTTATCCAGGAACGGGTTTTCAGATGCTCTGCCTGAGACCCTTGCCAGGGGCATTCCTTCTCTCTTGTCCATAGACTTGTCTTACAACAATCTCGGTTTAGAGAGCGTTCCATATTGGATCAGAAGCAGACAACTCAGAGATGTTCATCTTGCTGGCTGTCAATTGAGAGGATCCCTCCCAACATTTACAATGCCTGATTCCTTGACCTCTATTGACCTCTCTCACAATCATTTTACCGGTGGAATCTCAGAGCTCCTCACCAACGTGACAAGCTTGCAGAGCCTCAATCTCTCCAACAACCAATTGAAGTCGAATCTTTCAGAAATCAAATTGCCTGACGCAATTTCTTCCGTTGATGTGCACTCAAATCAGCTCGTGGGATCTCTCTCAAGAATCTTAAACGACAGGACAAGCAGTTTTTTGGAGTTTTTGGATGTCTCCCATAACCAAATTTCAGGTGAAATTCCAGAGTTTAAGGCAGGCTTGAGGCTCAAAGTGCTCCACATGGGAAGCAACAAGATTTCCGGTCACATCCCCGATTCAGTTTCAAACCTGACTCAACTTGAAAGGTTTGATGTATCAAGGAACCAGATGACAGGCACCATCCCTACAAGTTTGGGGTTGCTGGTGAAACTGAAATGGCTCGACGTGTCCGTCAATGGGCTCACAGGAAGGATTCCAAATAGCCTATTGGGGATTGAAGGGCTCAGACACGCAAGCTTCAGGGCAAACAGGTTATGCGGAGAGATCCCACAAGGGAGACCATTCAACATTTTCCCTGCAGCTGCTTACGTGCACAATTTGTGCTTATGTGGCAAGCCAATGCCACCTTGCAGGGGCAGGGGAAAGAAACAAGAAGCAAATATGATGAGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1052

Amino Acids

116.19

Weight (kDa)

8.3

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6817 PF20680 47 - 87 3.1e-10 Domain of unknown function (DUF6817)
LRRNT_2 PF08263 487 - 527 4.1e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 551 - 640 4.1e-07 Leucine-rich repeat region
LRR_8 PF13855 572 - 620 7.5e-06 Leucine rich repeat
LRR_14 PF23598 654 - 864 2.8e-09 Leucine-rich repeat region
LRR_8 PF13855 710 - 764 2.5e-08 Leucine rich repeat
LRR_8 PF13855 730 - 790 8e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23400
fragaria_vesca FvH4_4g37290
malus_domestica MD13G1001800.v1.1
prunus_persica Prupe.1G350800_v2.0.a1
pyrus_communis pycom13g00140 pycom16g00040
rosa_chinensis RchiOBHm_Chr4g0447111
rosa_laevigata RLG00000005604
rosa_multiflora Rmu_sc0010825.1_g000008 Rmu_ssc0000178.1_g000011
rosa_roxburghii Rroxscaffold_5G00387450
rosa_rugosa Rorug04G0378300
rosa_samantha Rh4AG439400 Rh4BG448500 Rh4CG465500 Rh4DG446900
rosa_wichuraiana Rw4G037590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 862
AasI GACNNNNNNGTC 1 cut(s) 2930
Acc36I ACCTGC 1 cut(s) 862
Acc65I GGTACC 1 cut(s) 2268
AccB1I GGYRCC 5 cut(s) 47, 182, 877, 2268, 2876
AccII CGCG 2 cut(s) 377, 687
AccIII TCCGGA 1 cut(s) 1625
AclWI GGATC 5 cut(s) 2402, 2442, 2455, 2665, 3019
AcoI YGGCCR 4 cut(s) 135, 640, 1235, 1864
AcsI RAATTY 6 cut(s) 712, 1497, 1675, 1960, 2727, 2739
AcuI CTGAAG 3 cut(s) 1026, 2089, 2983
AfaI GTAC 4 cut(s) 796, 1689, 1979, 2270
AflIII ACRYGT 1 cut(s) 2925
AgeI ACCGGT 1 cut(s) 2513
AhlI ACTAGT 1 cut(s) 791
AjiI CACGTC 2 cut(s) 1631, 2926
AjnI CCWGG 6 cut(s) 612, 697, 1247, 1582, 2282, 2321
AjuI GAANNNNNNNTTGG 2 cut(s) 1661, 1693
AleI CACNNNNGTG 1 cut(s) 2540
Alw21I GWGCWC 8 cut(s) 384, 624, 676, 1209, 2532, 2640, 2778, 3075
Alw26I GTCTC 5 cut(s) 975, 1536, 2306, 2719, 3031
Alw44I GTGCAC 2 cut(s) 2636, 3071
AlwI GGATC 5 cut(s) 2402, 2442, 2455, 2665, 3019
AlwNI CAGNNNCTG 3 cut(s) 424, 1233, 1545
Ama87I CYCGRG 1 cut(s) 647
Aor13HI TCCGGA 1 cut(s) 1625
ApaLI GTGCAC 2 cut(s) 2636, 3071
ApoI RAATTY 6 cut(s) 712, 1497, 1675, 1960, 2727, 2739
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
AsiGI ACCGGT 1 cut(s) 2513
Asp700I GAANNNNTTC 1 cut(s) 1040
Asp718I GGTACC 1 cut(s) 2268
AspA2I CCTAGG 1 cut(s) 1882
AspLEI GCGC 4 cut(s) 147, 1553, 1617, 2213
AspS9I GGNCC 9 cut(s) 235, 681, 1267, 1315, 1721, 1793, 1937, 2009, 2125
AsuC2I CCSGG 3 cut(s) 526, 838, 1135
AsuHPI GGTGA 9 cut(s) 107, 361, 839, 1750, 2185, 2218, 2527, 2747, 2917
AvaI CYCGRG 1 cut(s) 647
AvaII GGWCC 4 cut(s) 1315, 1721, 1937, 2125
AvrII CCTAGG 1 cut(s) 1882
AxyI CCTNAGG 1 cut(s) 89
BaeGI GKGCMC 3 cut(s) 280, 2640, 3075
BalI TGGCCA 1 cut(s) 1237
BamHI GGATCC 1 cut(s) 2447
BanI GGYRCC 5 cut(s) 47, 182, 877, 2268, 2876
BanII GRGCYC 6 cut(s) 384, 624, 676, 2532, 2943, 2985
BauI CACGAG 2 cut(s) 383, 2651
BbsI GAAGAC 3 cut(s) 867, 1518, 1782
Bbv12I GWGCWC 8 cut(s) 384, 624, 676, 1209, 2532, 2640, 2778, 3075
BbvCI CCTCAGC 1 cut(s) 1844
BceAI ACGGC 4 cut(s) 58, 122, 196, 1800
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 6 cut(s) 614, 699, 1249, 1584, 2284, 2323
BciVI GTATCC 1 cut(s) 2251
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 3 cut(s) 526, 838, 1135
BcoDI GTCTC 5 cut(s) 975, 1536, 2306, 2719, 3031
BcuI ACTAGT 1 cut(s) 791
BfaI CTAG 4 cut(s) 792, 1811, 1883, 1910
BfmI CTRYAG 4 cut(s) 416, 425, 1973, 3057
BfoI RGCGCY 2 cut(s) 1618, 2214
BfuAI ACCTGC 1 cut(s) 862
BfuI GTATCC 1 cut(s) 2251
BglI GCCNNNNNGGC 1 cut(s) 45
BlnI CCTAGG 1 cut(s) 1882
BmcAI AGTACT 1 cut(s) 796
Bme1390I CCNGG 9 cut(s) 526, 614, 699, 838, 1135, 1249, 1584, 2284, 2323
Bme18I GGWCC 4 cut(s) 1315, 1721, 1937, 2125
BmeT110I CYCGRG 1 cut(s) 647
BmgBI CACGTC 2 cut(s) 1631, 2926
BmgT120I GGNCC 9 cut(s) 235, 681, 1267, 1315, 1721, 1793, 1937, 2009, 2125
BmrFI CCNGG 9 cut(s) 526, 614, 699, 838, 1135, 1249, 1584, 2284, 2323
BmrI ACTGGG 1 cut(s) 958
BmsI GCATC 7 cut(s) 400, 904, 1128, 1263, 2079, 2166, 2290
BmuI ACTGGG 1 cut(s) 958
BpiI GAAGAC 3 cut(s) 867, 1518, 1782
BplI GAGNNNNNCTC 2 cut(s) 2436, 2468
BpmI CTGGAG 2 cut(s) 122, 1814
Bpu10I CCTNAGC 1 cut(s) 1844
BpuEI CTTGAG 3 cut(s) 206, 2650, 2782
BpuMI CCSGG 3 cut(s) 526, 838, 1135
BsaAI YACGTR 1 cut(s) 3070
BsaBI GATNNNNATC 2 cut(s) 770, 2421
BsaI GGTCTC 2 cut(s) 2306, 3031
BsaJI CCNNGG 9 cut(s) 40, 525, 579, 837, 1045, 1248, 1582, 1882, 2322
BsaWI WCCGGW 5 cut(s) 1625, 1805, 2236, 2513, 2801
BsaXI ACNNNNNCTCC 2 cut(s) 752, 782
Bse118I RCCGGY 2 cut(s) 1312, 2513
Bse1I ACTGG 5 cut(s) 884, 953, 2074, 2158, 3151
Bse21I CCTNAGG 1 cut(s) 89
Bse3DI GCAATG 2 cut(s) 1385, 2094
Bse8I GATNNNNATC 2 cut(s) 770, 2421
BseAI TCCGGA 1 cut(s) 1625
BseBI CCWGG 6 cut(s) 614, 699, 1249, 1584, 2284, 2323
BseDI CCNNGG 9 cut(s) 40, 525, 579, 837, 1045, 1248, 1582, 1882, 2322
BseJI GATNNNNATC 2 cut(s) 770, 2421
BseMI GCAATG 2 cut(s) 1385, 2094
BseNI ACTGG 5 cut(s) 884, 953, 2074, 2158, 3151
BseSI GKGCMC 3 cut(s) 280, 2640, 3075
BseYI CCCAGC 2 cut(s) 1560, 1618
Bsh1236I CGCG 2 cut(s) 377, 687
BshNI GGYRCC 5 cut(s) 47, 182, 877, 2268, 2876
BshTI ACCGGT 1 cut(s) 2513
BsiHKAI GWGCWC 8 cut(s) 384, 624, 676, 1209, 2532, 2640, 2778, 3075
BsiHKCI CYCGRG 1 cut(s) 647
BslFI GGGAC 3 cut(s) 311, 1175, 1660
BsmAI GTCTC 5 cut(s) 975, 1536, 2306, 2719, 3031
BsmFI GGGAC 3 cut(s) 311, 1175, 1660
BsmI GAATGC 4 cut(s) 216, 838, 1314, 2328
Bso31I GGTCTC 2 cut(s) 2306, 3031
BsoBI CYCGRG 1 cut(s) 647
Bsp13I TCCGGA 1 cut(s) 1625
Bsp143I GATC 6 cut(s) 595, 2059, 2394, 2447, 2657, 3024
Bsp19I CCATGG 1 cut(s) 579
BspEI TCCGGA 1 cut(s) 1625
BspFNI CGCG 2 cut(s) 377, 687
BspHI TCATGA 1 cut(s) 1212
BspMAI CTGCAG 4 cut(s) 420, 429, 1977, 3061
BspMI ACCTGC 1 cut(s) 862
BspPI GGATC 5 cut(s) 2402, 2442, 2455, 2665, 3019
BspQI GCTCTTC 1 cut(s) 915
BspT107I GGYRCC 5 cut(s) 47, 182, 877, 2268, 2876
BspTNI GGTCTC 2 cut(s) 2306, 3031
BsrDI GCAATG 2 cut(s) 1385, 2094
BsrFI RCCGGY 2 cut(s) 1312, 2513
BsrI ACTGG 5 cut(s) 884, 953, 2074, 2158, 3151
BssAI RCCGGY 2 cut(s) 1312, 2513
BssECI CCNNGG 9 cut(s) 40, 525, 579, 837, 1045, 1248, 1582, 1882, 2322
BssMI GATC 6 cut(s) 595, 2059, 2394, 2447, 2657, 3024
BssSI CACGAG 2 cut(s) 383, 2651
BssT1I CCWWGG 3 cut(s) 579, 1045, 1882
Bst2BI CACGAG 2 cut(s) 383, 2651
Bst2UI CCWGG 6 cut(s) 614, 699, 1249, 1584, 2284, 2323
Bst4CI ACNGT 4 cut(s) 24, 508, 1600, 1856
Bst6I CTCTTC 3 cut(s) 915, 1002, 2108
BstAPI GCANNNNNTGC 3 cut(s) 424, 877, 1545
BstBAI YACGTR 1 cut(s) 3070
BstDSI CCRYGG 2 cut(s) 40, 579
BstENI CCTNNNNNAGG 2 cut(s) 88, 1214
BstFNI CGCG 2 cut(s) 377, 687
BstH2I RGCGCY 2 cut(s) 1618, 2214
BstHHI GCGC 4 cut(s) 147, 1553, 1617, 2213
BstKTI GATC 6 cut(s) 598, 2062, 2397, 2450, 2660, 3027
BstMAI GTCTC 5 cut(s) 975, 1536, 2306, 2719, 3031
BstMBI GATC 6 cut(s) 595, 2059, 2394, 2447, 2657, 3024
BstNI CCWGG 6 cut(s) 614, 699, 1249, 1584, 2284, 2323
BstNSI RCATGY 2 cut(s) 697, 1061
BstSCI CCNGG 9 cut(s) 524, 612, 697, 836, 1133, 1247, 1582, 2282, 2321
BstSFI CTRYAG 4 cut(s) 416, 425, 1973, 3057
BstSLI GKGCMC 3 cut(s) 280, 2640, 3075
BstUI CGCG 2 cut(s) 377, 687
BstV2I GAAGAC 3 cut(s) 867, 1518, 1782
BstX2I RGATCY 3 cut(s) 2447, 2657, 3024
BstXI CCANNNNNNTGG 4 cut(s) 705, 2081, 2122, 2153
BstYI RGATCY 3 cut(s) 2447, 2657, 3024
Bsu36I CCTNAGG 1 cut(s) 89
BsuI GTATCC 1 cut(s) 2251
BtgI CCRYGG 2 cut(s) 40, 579
BtgZI GCGATG 2 cut(s) 702, 1363
BtrI CACGTC 2 cut(s) 1631, 2926
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 5 cut(s) 567, 598, 891, 1434, 1705
BveI ACCTGC 1 cut(s) 862
CaiI CAGNNNCTG 3 cut(s) 424, 1233, 1545
CciI TCATGA 1 cut(s) 1212
CfoI GCGC 4 cut(s) 147, 1553, 1617, 2213
Cfr10I RCCGGY 2 cut(s) 1312, 2513
Cfr13I GGNCC 9 cut(s) 235, 681, 1267, 1315, 1721, 1793, 1937, 2009, 2125
CseI GACGC 2 cut(s) 383, 2624
Csp6I GTAC 4 cut(s) 795, 1688, 1978, 2269
CspAI ACCGGT 1 cut(s) 2513
CspCI CAANNNNNGTGG 2 cut(s) 1567, 1602
CviQI GTAC 4 cut(s) 795, 1688, 1978, 2269
DpnI GATC 6 cut(s) 597, 2061, 2396, 2449, 2659, 3026
DpnII GATC 6 cut(s) 595, 2059, 2394, 2447, 2657, 3024
DrdI GACNNNNNNGTC 1 cut(s) 2930
DseDI GACNNNNNNGTC 1 cut(s) 2930
EaeI YGGCCR 4 cut(s) 135, 640, 1235, 1864
Eam1104I CTCTTC 3 cut(s) 915, 1002, 2108
EarI CTCTTC 3 cut(s) 915, 1002, 2108
EciI GGCGGA 2 cut(s) 857, 1121
Ecl136II GAGCTC 4 cut(s) 382, 622, 674, 2530
Eco130I CCWWGG 3 cut(s) 579, 1045, 1882
Eco24I GRGCYC 6 cut(s) 384, 624, 676, 2532, 2943, 2985
Eco31I GGTCTC 2 cut(s) 2306, 3031
Eco47I GGWCC 4 cut(s) 1315, 1721, 1937, 2125
Eco53kI GAGCTC 4 cut(s) 382, 622, 674, 2530
Eco57I CTGAAG 3 cut(s) 1026, 2089, 2983
Eco81I CCTNAGG 1 cut(s) 89
Eco88I CYCGRG 1 cut(s) 647
EcoICRI GAGCTC 4 cut(s) 382, 622, 674, 2530
EcoNI CCTNNNNNAGG 2 cut(s) 88, 1214
EcoO109I RGGNCCY 2 cut(s) 235, 1937
EcoRI GAATTC 1 cut(s) 712
EcoRII CCWGG 6 cut(s) 612, 697, 1247, 1582, 2282, 2321
EcoT14I CCWWGG 3 cut(s) 579, 1045, 1882
EcoT38I GRGCYC 6 cut(s) 384, 624, 676, 2532, 2943, 2985
ErhI CCWWGG 3 cut(s) 579, 1045, 1882
FalI AAGNNNNNCTT 2 cut(s) 2744, 2776
FaqI GGGAC 3 cut(s) 311, 1175, 1660
FauI CCCGC 2 cut(s) 86, 692
FbaI TGATCA 1 cut(s) 595
FriOI GRGCYC 6 cut(s) 384, 624, 676, 2532, 2943, 2985
FspBI CTAG 4 cut(s) 792, 1811, 1883, 1910
GlaI GCGC 4 cut(s) 146, 1552, 1616, 2212
GsaI CCCAGC 2 cut(s) 1564, 1622
GsuI CTGGAG 2 cut(s) 122, 1814
HaeII RGCGCY 2 cut(s) 1618, 2214
HgaI GACGC 2 cut(s) 383, 2624
HhaI GCGC 4 cut(s) 147, 1553, 1617, 2213
Hin6I GCGC 4 cut(s) 145, 1551, 1615, 2211
HinP1I GCGC 4 cut(s) 145, 1551, 1615, 2211
HincII GTYRAC 3 cut(s) 112, 205, 1261
HindII GTYRAC 3 cut(s) 112, 205, 1261
HindIII AAGCTT 3 cut(s) 2117, 2548, 2995
HphI GGTGA 9 cut(s) 107, 361, 839, 1750, 2185, 2218, 2527, 2747, 2917
Hpy166II GTNNAC 6 cut(s) 112, 205, 1261, 1533, 2638, 3073
Hpy8I GTNNAC 6 cut(s) 112, 205, 1261, 1533, 2638, 3073
Hpy99I CGWCG 5 cut(s) 28, 353, 377, 1438, 2927
HpyCH4III ACNGT 4 cut(s) 24, 508, 1600, 1856
HpyCH4IV ACGT 5 cut(s) 1630, 2230, 2541, 2925, 3069
HpySE526I ACGT 5 cut(s) 1630, 2230, 2541, 2925, 3069
HspAI GCGC 4 cut(s) 145, 1551, 1615, 2211
Kpn2I TCCGGA 1 cut(s) 1625
KpnI GGTACC 1 cut(s) 2272
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 6 cut(s) 595, 2059, 2394, 2447, 2657, 3024
LguI GCTCTTC 1 cut(s) 915
LmnI GCTCC 8 cut(s) 155, 379, 388, 671, 1051, 1212, 2535, 2781
LweI GCATC 7 cut(s) 400, 904, 1128, 1263, 2079, 2166, 2290
MaeI CTAG 4 cut(s) 792, 1811, 1883, 1910
MaeII ACGT 5 cut(s) 1630, 2230, 2541, 2925, 3069
MaeIII GTNAC 4 cut(s) 502, 1594, 2542, 2804
MalI GATC 6 cut(s) 597, 2061, 2396, 2449, 2659, 3026
MboI GATC 6 cut(s) 595, 2059, 2394, 2447, 2657, 3024
MfeI CAATTG 2 cut(s) 2438, 2579
MflI RGATCY 3 cut(s) 2447, 2657, 3024
MlsI TGGCCA 1 cut(s) 1237
MluNI TGGCCA 1 cut(s) 1237
MlyI GAGTC 6 cut(s) 113, 236, 490, 1642, 2175, 2827
MmeI TCCRAC 6 cut(s) 9, 133, 443, 1401, 1930, 2595
Mox20I TGGCCA 1 cut(s) 1237
MroI TCCGGA 1 cut(s) 1625
MroXI GAANNNNTTC 1 cut(s) 1040
MscI TGGCCA 1 cut(s) 1237
MseI TTAA 3 cut(s) 2255, 2675, 2751
MslI CAYNNNNRTG 2 cut(s) 1342, 2540
Msp20I TGGCCA 1 cut(s) 1237
MspA1I CMGCKG 5 cut(s) 424, 1848, 1863, 2038, 3062
MspR9I CCNGG 9 cut(s) 526, 614, 699, 838, 1135, 1249, 1584, 2284, 2323
MunI CAATTG 2 cut(s) 2438, 2579
Mva1269I GAATGC 4 cut(s) 216, 838, 1314, 2328
MvaI CCWGG 6 cut(s) 614, 699, 1249, 1584, 2284, 2323
MvnI CGCG 2 cut(s) 377, 687
NciI CCSGG 3 cut(s) 526, 838, 1135
NcoI CCATGG 1 cut(s) 579
NdeII GATC 6 cut(s) 595, 2059, 2394, 2447, 2657, 3024
NmuCI GTSAC 2 cut(s) 2542, 2804
NspI RCATGY 2 cut(s) 697, 1061
OliI CACNNNNGTG 1 cut(s) 2540
PaeI GCATGC 1 cut(s) 697
PaeR7I CTCGAG 1 cut(s) 647
PagI TCATGA 1 cut(s) 1212
PaqCI CACCTGC 1 cut(s) 862
PciSI GCTCTTC 1 cut(s) 915
PcsI WCGNNNNNNNCGW 3 cut(s) 198, 381, 2928
PctI GAATGC 4 cut(s) 216, 838, 1314, 2328
PdmI GAANNNNTTC 1 cut(s) 1040
PfeI GAWTC 8 cut(s) 307, 1373, 2474, 2520, 2590, 2670, 2816, 2953
PflFI GACNNNGTC 1 cut(s) 245
PfoI TCCNGGA 2 cut(s) 612, 2282
PinAI ACCGGT 1 cut(s) 2513
PleI GAGTC 6 cut(s) 112, 236, 489, 1642, 2175, 2827
PpsI GAGTC 6 cut(s) 112, 236, 489, 1642, 2175, 2827
Ppu21I YACGTR 1 cut(s) 3070
PpuMI RGGWCCY 1 cut(s) 1937
Psp124BI GAGCTC 4 cut(s) 384, 624, 676, 2532
Psp5II RGGWCCY 1 cut(s) 1937
Psp6I CCWGG 6 cut(s) 612, 697, 1247, 1582, 2282, 2321
PspFI CCCAGC 2 cut(s) 1560, 1618
PspGI CCWGG 6 cut(s) 612, 697, 1247, 1582, 2282, 2321
PspPI GGNCC 9 cut(s) 235, 681, 1267, 1315, 1721, 1793, 1937, 2009, 2125
PspPPI RGGWCCY 1 cut(s) 1937
PspXI VCTCGAGB 1 cut(s) 647
PstI CTGCAG 4 cut(s) 420, 429, 1977, 3061
PstNI CAGNNNCTG 3 cut(s) 424, 1233, 1545
PsuI RGATCY 3 cut(s) 2447, 2657, 3024
PsyI GACNNNGTC 1 cut(s) 245
PvuII CAGCTG 3 cut(s) 424, 2038, 3062
RsaI GTAC 4 cut(s) 796, 1689, 1979, 2270
RsaNI GTAC 4 cut(s) 795, 1688, 1978, 2269
RseI CAYNNNNRTG 2 cut(s) 1342, 2540
SacI GAGCTC 4 cut(s) 384, 624, 676, 2532
SapI GCTCTTC 1 cut(s) 915
SaqAI TTAA 3 cut(s) 2255, 2675, 2751
Sau3AI GATC 6 cut(s) 595, 2059, 2394, 2447, 2657, 3024
Sau96I GGNCC 9 cut(s) 235, 681, 1267, 1315, 1721, 1793, 1937, 2009, 2125
ScaI AGTACT 1 cut(s) 796
SchI GAGTC 6 cut(s) 113, 236, 490, 1642, 2175, 2827
ScrFI CCNGG 9 cut(s) 526, 614, 699, 838, 1135, 1249, 1584, 2284, 2323
SfaNI GCATC 7 cut(s) 400, 904, 1128, 1263, 2079, 2166, 2290
SfcI CTRYAG 4 cut(s) 416, 425, 1973, 3057
Sfr274I CTCGAG 1 cut(s) 647
SinI GGWCC 4 cut(s) 1315, 1721, 1937, 2125
SlaI CTCGAG 1 cut(s) 647
SmiMI CAYNNNNRTG 2 cut(s) 1342, 2540
SmlI CTYRAG 4 cut(s) 221, 647, 2665, 2761
SmoI CTYRAG 4 cut(s) 221, 647, 2665, 2761
SpeI ACTAGT 1 cut(s) 791
SphI GCATGC 1 cut(s) 697
SspMI CTAG 4 cut(s) 792, 1811, 1883, 1910
SstI GAGCTC 4 cut(s) 384, 624, 676, 2532
StyD4I CCNGG 9 cut(s) 524, 612, 697, 836, 1133, 1247, 1582, 2282, 2321
StyI CCWWGG 3 cut(s) 579, 1045, 1882
TaaI ACNGT 4 cut(s) 24, 508, 1600, 1856
TaiI ACGT 5 cut(s) 1633, 2233, 2544, 2928, 3072
TaqI TCGA 9 cut(s) 74, 192, 545, 648, 867, 1773, 2218, 2588, 2922
TatI WGTACW 1 cut(s) 794
TauI GCSGC 7 cut(s) 48, 266, 446, 645, 1057, 1851, 1866
TfiI GAWTC 8 cut(s) 307, 1373, 2474, 2520, 2590, 2670, 2816, 2953
Tru1I TTAA 3 cut(s) 2255, 2675, 2751
Tru9I TTAA 3 cut(s) 2255, 2675, 2751
TscAI CASTG 5 cut(s) 567, 598, 891, 1434, 1705
TseFI GTSAC 2 cut(s) 2542, 2804
Tsp45I GTSAC 2 cut(s) 2542, 2804
TspDTI ATGAA 5 cut(s) 725, 873, 1229, 1655, 2411
TspGWI ACGGA 4 cut(s) 97, 259, 2617, 2920
TspRI CASTG 5 cut(s) 567, 598, 891, 1434, 1705
Tth111I GACNNNGTC 1 cut(s) 245
VneI GTGCAC 2 cut(s) 2636, 3071
VpaK11BI GGWCC 4 cut(s) 1315, 1721, 1937, 2125
XagI CCTNNNNNAGG 2 cut(s) 88, 1214
XapI RAATTY 6 cut(s) 712, 1497, 1675, 1960, 2727, 2739
XceI RCATGY 2 cut(s) 697, 1061
XcmI CCANNNNNNNNNTGG 2 cut(s) 724, 1245
XhoI CTCGAG 1 cut(s) 647
XmaJI CCTAGG 1 cut(s) 1882
XmnI GAANNNNTTC 1 cut(s) 1040
XspI CTAG 4 cut(s) 792, 1811, 1883, 1910
ZrmI AGTACT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.