Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0015312

Physical Location & Seq

Genomic Coordinates Forward (+)

723508 .. 728043

Gene Structure 4536 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3885 bp

ATGCCAAATGCCATTAACCCCCAGACTGCCTTTGATGATGTTGCCTCCTCCTGTATCTTGGCTGTTGATTCATATACACAAACCAATATTATTTATATGAACCATTTTTTTAAGGATATAGCGCCGATCTTAGATAAAACCTCAATCTCTCACTACTTTCTCCTCTCTCTCTCTCTACAGTTCATGGTACTCGAACCGTATAAGTACAAACCCACCACCAACCACATTCTAATTCCCTTCTTTTCTTATTCCTTTCCACCTCCCTCCTTTCCTTTCTCACAACATTTTCTCATGATATGCACTCACTCTCACCACTCACTCCCTCACACTCTCTCTAAATTGTCTTCCGGAGAATTGAAACCTGTTTCGTCAATGGAGAACTTCAATCCAGTCCAGGTTTTTCTTCATCTGTCTGCAATACTTGTATTGGTAGGACTTCACTTTGTTTCTGTTTCCACAGCTGATCAACAAGTCACTCCATCGATCAAAACCGACGCAGTTTCTCTTCTCAACTTCAAAAAGATGATTGACACGGACCCGAATGGCGTCTTGTCGGGTTGGAAGCTCGGCACCAGCCCATGCAGCTGGTATGGAGTTACATGCTCCATGAACCGAGCAACTCAGCTCGATCTTAGCGGTTGTTTTCTTGTGGGGACAATCTCCTTTGCACCCTTGGCCTCTCTAGACATGCTTTCTGTCTTAAATCTGCCTTCCAATAAATTCAGTATAAACTCAACCTCTTTGCTTCAACTCCCATATGCTTTGAAACAGCTCGATTTATCTTTCAACGCACTTTTCGGTGTTCTTCCTGAGACTCTTTCCTCAAAATGTCCAAATCTTGTCTCTGTGAATCTTGCTTTTAACAACTTAACCGGCTCTCTACCCAAAGATCTCTTATTGAATTCCGACAAACTTCAAACCCTCGACATCTCTTACAACAATCTAACCGGGTCGATTTCTGGTCTGAGAATTGAGAAGTATTCTTGCCCTTCTTTGTTGCAGCTTGATTTGTCAGGAAACCGTATTAGGGGTTCCATTCCTGTGTCCTTGGCAAACTGCACCAGTCTCAAAACTATGAGTTTATCGACCAACAATGTGACAGGCGAAATCCCAGCATCGTTTGGGCAACTGGGCAGTCTACAGAGATTGGATCTTTCTCACAATCAGATAACCGGTTGGATACCTCCTGAATTAGGAAATGCATGCGCTCTGCTTGTCGAACTCAAGCTTTCGTTTAACAACATTACTGGTCCAATTCCAGCCTCTTTCTCCTCGTGTTCTTCGCTGGAGCTTCTCGATCTCTCTAACAACAACTTGACAGGTCCCCTACCAGATTCTATCTTTCAGAACCTCACCTCCTTACAGAGCTTGGTGTTGAGGAATAACATCATTTCTGGATCACTTCCGGGTTCCATATCAGCTTGCAAAAGCCTGCAGGTTATAGACTTGAGTTCTAATAAAATATCCGGTGTCATCCCACCAGATATATGTCCAGGAGCCTCGTCACTTGAGGAGCTGAGAATGCCGGACAACCTCATTGTAGGCGAAATCCCTGCTCAACTGTCACAATGTTCTCAGCTGAAGACGATCGATCTTAGTTTGAACTATCTCAACGGCTCGATTCCAGCTGAGCTTGGGAAGCTGGAGAATTTGCAGCAGCTAATAGCATGGTACAATGGCTTAGAGGGGAAAATCCCACCAGACTTGGGAAAATGCAAGAATCTCATCGATCTTATTCTCAATAATAACAATCTGAGTGGTGCAATCCCAGTTGAATTGTTCAGCTGCAGCAATCTTGAATGGATATCACTCACAAGCAATCAACTCAGTGGTGAAATCCCAGGGGAATTTGGCCTATTGACACGACTGGCGGTTCTACAACTTGGGAACAATAGCTTGAGTGGCAAGATACCAGGAGAGCTCGGAAATTGCAGCAGTTTGGTTTGGTTGGATTTGAACAGCAACAGACTCACCGGAGAGATCCCACCTCGACTTGGGAGGCAGATTGGAGCTAAAGCACTGAGCGGAATACTCTCCGGCAATACTTTGGTGTTTGTACGGAATGTGGGAAACTCTTGTAAAGGAGTGGGAGGCTTATTAGAGTTTGCAGGAATCCGACCTGAAAGGCTTCAGCAGGACCCAACATTGAGGACTTGTGACTTCGCCAGATTGTACTCTGGTCCAGTGCTGAGTCTCTTTACAAAATACCAAACACTGGAGTATCTTGATCGATCTATCATACAATCAGCTTCGCGGGAAAATCCCGGAAGAAATGGGGGACATGATTGTGAGATTCCTGCATCACTTGGCCAGCTCAAAGATTTAGGGGTGTTTGACGCATCACATAACAGACTGCAGGGTCATATCCCCGATTCTTTGGAGAACCTATCTTTCTTGGTGCAAATTGATTTGTCCAACAATGAGTTAACCGGAGAAATTCCCTCTAGGGGTCAGCTGAGTACACTTCCTGCTACCCAGTATGCTAACAACCCTGGACTCTGTGGGGTCCCATTGCCCGATTGCCAGAGCAGCAATGACCAACCATCCACGACTCCGAGCGATCAGGATGCAGGCAAACAAAGTCGCAGGCCATCAGTTGCATCATTGGCTAACAGCATTGTCGTGGGGGTTCTGATTTCTCTTGCTTCGGTCTGTGTTCTGATTGTGTGGGCAATTGCAATGCGCACAAGGCGAAAGGAAGCAAAGGAGGTGAAGATGCTTAATCGCTTGCAAGCATCCCATGCGGCATGGAAGATTGACAAGGAGAAAGAACCCTTGAGCATTAATGTTGCAACTTTCCAAAGGCAGTTGAGGAAGCTCAAGTTTTCCCAACTCATTGAGGCAACCAATGGCTTCTCGGCAGATAGTCTTATCGGGTGTGGAGGTTTTGGGGAAGTGTTCAAGGCAACGCTGAAAGATGGGTCAAGTGTTGCAATCAAGAAACTTATACGGCTGAGCTGCCAAGGTGACCGTGAATTCATGCTTGTGCCTTTATTGGGTTATTGCAAAATTGGCGAAGAGAGGCTGCTAGTATATGAATTCATGGAGTACGGAAGCCTCGAAGAAATGCTCCATGGAAGAACAAAGACCCGTGATAGGCGGATGCTAACATGGGAGGAAAGGAAAAAGATTGCAAGAGGCGCGGCGAAGGGACTGTGTTTCCTCCACCACAATTGCATCCCTCACATCATACACAGAGATATGAAGTCAAGCAATGTATTGTTGGACAACGAAATGGAAGCAAGAGTTTCTGATTTTGGAATGGCAAGGCTCATAAGTGCTCTTGACACACACTTGAGTGTAAGCACTCTTGCAGGCACTCCTGGTTATGTTCCACCTGAATACTACCAGAGTTTCCGCTGCACTGCAAAGGGCGATGTCTACTCATTTGGGGTTGTCCTCCTGGAGCTTGTGACCGGAAAACGCCCAACAGACAAGGAAGATTTTGGGGACACTAACTTGGTCGGATGGGCGAAGATGAAGGTGAGAGAAGGGAAACAAATGGAAGTGATAGACCAAGAGTTACTTTCAGTAACTAAAGGAACGGATGAAGCAGAAGCTGACGAAGTGAAAGAGATGGTGAGGTATTTAGGGGTGACACTGCAGTGTGTCGACGACTTCCCATCAAAGAGGCCTAACATGTTGCAGGTGGTTGCCATGCTGAGAGAGCTGGTGCCTGGATCAGCAAGTGGAAGTAGTAACAGTCGATCACTCTTCATCATCATCGTTAATCACTGTAAGAATATTACAACATTTGTAAGAACCTTTTTGCAAATCTTTTCTTCTTCTAAGAGCAATAGAAGTGAAATTTTTGGCAAACGTTGGACAATGTCGGACACCCGGAGTTGCTTGTTTGGTAGGCAACCGCTGGATTTGAGTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0010051 GO:0010232 GO:0010233 GO:0010305 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048545 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1295

Amino Acids

141.53

Weight (kDa)

6.88

Isoelectric Point (pI)

38.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	164 - 202	8.1e-12	Leucine rich repeat N-terminal domain
LRR_4	PF12799	304 - 342	5.9e-06	Leucine Rich repeats (2 copies)
LRR_8	PF13855	330 - 390	6.1e-09	Leucine rich repeat
LRR_8	PF13855	405 - 462	1e-08	Leucine rich repeat
LRR_14	PF23598	421 - 558	2.1e-07	Leucine-rich repeat region
LRR_8	PF13855	443 - 488	2e-07	Leucine rich repeat
LRR_4	PF12799	452 - 489	5.5e-06	Leucine Rich repeats (2 copies)
LRR_8	PF13855	453 - 513	5.8e-07	Leucine rich repeat
Island	PF20141	674 - 734	1.1e-18	Brassinosteroid receptor island domain
Pkinase	PF00069	954 - 1158	4.2e-32	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	955 - 1220	5.6e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015312)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01950
fragaria_vesca	FvH4_5g27210
malus_domestica	MD13G1014800.v1.1
prunus_persica	Prupe.1G338800_v2.0.a1
pyrus_communis	pycom13g01260 pycom16g01160
rosa_chinensis	RchiOBHm_Chr7g0218311
rosa_laevigata	RLG00000002379
rosa_multiflora	Rmu_sc0009526.1_g000007
rosa_roxburghii	Rroxscaffold_3G00241060
rosa_rugosa	Rorug07G0176700
rosa_samantha	Rh7AG318000 Rh7BG309700 Rh7CG334800 Rh7DG317000
rosa_wichuraiana	Rw7G027020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3637
AasI	GACNNNNNNGTC	1 cut(s)	2358
Acc16I	TGCGCA	1 cut(s)	2684
Acc36I	ACCTGC	2 cut(s)	1426, 3637
AccB1I	GGYRCC	2 cut(s)	569, 3673
AccB7I	CCANNNNNTGG	1 cut(s)	2215
AccBSI	CCGCTC	1 cut(s)	2025
AccI	GTMKAC	3 cut(s)	1138, 3381, 3612
AccII	CGCG	2 cut(s)	2254, 3143
AccIII	TCCGGA	1 cut(s)	347
AciI	CCGC	9 cut(s)	636, 1871, 2025, 2254, 2744, 3100, 3143, 3358, 3866
AclI	AACGTT	1 cut(s)	3820
AclWI	GGATC	4 cut(s)	1158, 1405, 1975, 3688
AcoI	YGGCCR	1 cut(s)	2308
AcsI	RAATTY	8 cut(s)	719, 901, 1648, 1847, 2436, 2975, 3038, 3807
AcuI	CTGAAG	2 cut(s)	1601, 2114
AcyI	GRCGYC	1 cut(s)	546
AfaI	GTAC	7 cut(s)	189, 206, 1673, 2058, 2174, 2461, 3050
AfiI	CCNNNNNNNGG	8 cut(s)	1027, 1193, 1706, 1994, 2215, 2447, 2996, 3096
AflIII	ACRYGT	1 cut(s)	3639
AgeI	ACCGGT	1 cut(s)	1172
AjnI	CCWGG	8 cut(s)	393, 1492, 1840, 1912, 2491, 3322, 3402, 3676
AleI	CACNNNNGTG	2 cut(s)	3297, 3604
AloI	GAACNNNNNNTCC	2 cut(s)	1340, 1372
Alw21I	GWGCWC	2 cut(s)	1923, 3283
Alw26I	GTCTC	4 cut(s)	806, 847, 1070, 2198
AlwI	GGATC	4 cut(s)	1158, 1405, 1975, 3688
AlwNI	CAGNNNCTG	1 cut(s)	3560
Aor13HI	TCCGGA	1 cut(s)	347
AoxI	GGCC	5 cut(s)	675, 1852, 2308, 2588, 3632
ApoI	RAATTY	8 cut(s)	719, 901, 1648, 1847, 2436, 2975, 3038, 3807
AseI	ATTAAT	1 cut(s)	2784
AsiGI	ACCGGT	1 cut(s)	1172
Asp700I	GAANNNNTTC	1 cut(s)	2127
AspLEI	GCGC	4 cut(s)	124, 1208, 2685, 3143
AspS9I	GGNCC	6 cut(s)	535, 1250, 1322, 2137, 2180, 2506
AsuC2I	CCSGG	4 cut(s)	949, 1407, 2265, 3841
AsuHPI	GGTGA	9 cut(s)	302, 1345, 1844, 1963, 2722, 2978, 3496, 3592, 3607
AvaII	GGWCC	6 cut(s)	535, 1250, 1322, 2137, 2180, 2506
BaeI	ACNNNNGTAYC	2 cut(s)	2204, 2237
BalI	TGGCCA	1 cut(s)	2310
BanI	GGYRCC	2 cut(s)	569, 3673
BanII	GRGCYC	1 cut(s)	1923
BauI	CACGAG	1 cut(s)	1273
BbsI	GAAGAC	2 cut(s)	336, 1589
Bbv12I	GWGCWC	2 cut(s)	1923, 3283
BccI	CCATC	7 cut(s)	487, 2551, 2599, 2912, 3462, 3571, 3631
BceAI	ACGGC	2 cut(s)	1630, 2966
BcgI	CGANNNNNNTGC	4 cut(s)	1535, 1569, 2549, 2583
BciT130I	CCWGG	8 cut(s)	395, 1494, 1842, 1914, 2493, 3324, 3404, 3678
BciVI	GTATCC	1 cut(s)	1173
BclI	TGATCA	1 cut(s)	463
BcnI	CCSGG	4 cut(s)	949, 1407, 2265, 3841
BcoDI	GTCTC	4 cut(s)	806, 847, 1070, 2198
BfaI	CTAG	3 cut(s)	683, 2445, 3029
BfmI	CTRYAG	6 cut(s)	176, 1139, 1433, 1786, 2354, 3602
BfoI	RGCGCY	1 cut(s)	125
BfuAI	ACCTGC	2 cut(s)	1426, 3637
BfuI	GTATCC	1 cut(s)	1173
BglII	AGATCT	1 cut(s)	889
BlpI	GCTNAGC	2 cut(s)	1629, 2954
Bme18I	GGWCC	6 cut(s)	535, 1250, 1322, 2137, 2180, 2506
BmgT120I	GGNCC	6 cut(s)	535, 1250, 1322, 2137, 2180, 2506
BmrI	ACTGGG	3 cut(s)	1139, 1763, 2470
BmsI	GCATC	9 cut(s)	1124, 2309, 2348, 2557, 2609, 2706, 2744, 3093, 3186
BmuI	ACTGGG	3 cut(s)	1139, 1763, 2470
BpiI	GAAGAC	2 cut(s)	336, 1589
BpmI	CTGGAG	4 cut(s)	1307, 1664, 2237, 3425
Bpu1102I	GCTNAGC	2 cut(s)	1629, 2954
BpuEI	CTTGAG	7 cut(s)	1208, 1468, 1529, 1918, 2797, 2804, 3316
BpuMI	CCSGG	4 cut(s)	949, 1407, 2265, 3841
Bsa29I	ATCGAT	4 cut(s)	482, 1590, 1728, 2230
BsaHI	GRCGYC	1 cut(s)	546
BsaJI	CCNNGG	7 cut(s)	672, 1047, 1840, 1841, 2491, 2962, 3073
BsaWI	WCCGGW	6 cut(s)	347, 1172, 1466, 1973, 2429, 3416
BsaXI	ACNNNNNCTCC	2 cut(s)	1340, 1370
Bsc4I	CCNNNNNNNGG	8 cut(s)	1027, 1193, 1706, 1994, 2215, 2447, 2996, 3096
Bse118I	RCCGGY	2 cut(s)	872, 1172
Bse1I	ACTGG	9 cut(s)	389, 1062, 1134, 1252, 1769, 1872, 2183, 2220, 2476
Bse3DI	GCAATG	4 cut(s)	2510, 2539, 2685, 3220
BseAI	TCCGGA	1 cut(s)	347
BseBI	CCWGG	8 cut(s)	395, 1494, 1842, 1914, 2493, 3324, 3404, 3678
BseCI	ATCGAT	4 cut(s)	482, 1590, 1728, 2230
BseDI	CCNNGG	7 cut(s)	672, 1047, 1840, 1841, 2491, 2962, 3073
BseGI	GGATG	8 cut(s)	1473, 2543, 2572, 2735, 3108, 3177, 3473, 3553
BseLI	CCNNNNNNNGG	8 cut(s)	1027, 1193, 1706, 1994, 2215, 2447, 2996, 3096
BseMI	GCAATG	4 cut(s)	2510, 2539, 2685, 3220
BseNI	ACTGG	9 cut(s)	389, 1062, 1134, 1252, 1769, 1872, 2183, 2220, 2476
BseRI	GAGGAG	4 cut(s)	37, 152, 1261, 1526
BseYI	CCCAGC	1 cut(s)	1111
BsgI	GTGCAG	2 cut(s)	1042, 3346
Bsh1236I	CGCG	2 cut(s)	2254, 3143
Bsh1285I	CGRYCG	1 cut(s)	1590
BshFI	GGCC	5 cut(s)	677, 1854, 2310, 2590, 3634
BshNI	GGYRCC	2 cut(s)	569, 3673
BshTI	ACCGGT	1 cut(s)	1172
BshVI	ATCGAT	4 cut(s)	482, 1590, 1728, 2230
BsiEI	CGRYCG	1 cut(s)	1590
BsiHKAI	GWGCWC	2 cut(s)	1923, 3283
BslFI	GGGAC	6 cut(s)	667, 1308, 2292, 2492, 3165, 3464
BslI	CCNNNNNNNGG	8 cut(s)	1027, 1193, 1706, 1994, 2215, 2447, 2996, 3096
BsmAI	GTCTC	4 cut(s)	806, 847, 1070, 2198
BsmFI	GGGAC	6 cut(s)	667, 1308, 2292, 2492, 3165, 3464
BsmI	GAATGC	1 cut(s)	1527
BsnI	GGCC	5 cut(s)	677, 1854, 2310, 2590, 3634
Bsp1286I	GDGCHC	2 cut(s)	1923, 3283
Bsp13I	TCCGGA	1 cut(s)	347
Bsp1720I	GCTNAGC	2 cut(s)	1629, 2954
Bsp19I	CCATGG	1 cut(s)	3073
BspACI	CCGC	9 cut(s)	636, 1871, 2025, 2254, 2744, 3100, 3143, 3358, 3866
BspANI	GGCC	5 cut(s)	677, 1854, 2310, 2590, 3634
BspDI	ATCGAT	4 cut(s)	482, 1590, 1728, 2230
BspEI	TCCGGA	1 cut(s)	347
BspFNI	CGCG	2 cut(s)	2254, 3143
BspHI	TCATGA	1 cut(s)	291
BspMAI	CTGCAG	4 cut(s)	1437, 1790, 2358, 3606
BspMI	ACCTGC	2 cut(s)	1426, 3637
BspPI	GGATC	4 cut(s)	1158, 1405, 1975, 3688
BspT107I	GGYRCC	2 cut(s)	569, 3673
BsrBI	CCGCTC	1 cut(s)	2025
BsrDI	GCAATG	4 cut(s)	2510, 2539, 2685, 3220
BsrFI	RCCGGY	2 cut(s)	872, 1172
BsrI	ACTGG	9 cut(s)	389, 1062, 1134, 1252, 1769, 1872, 2183, 2220, 2476
BssAI	RCCGGY	2 cut(s)	872, 1172
BssECI	CCNNGG	7 cut(s)	672, 1047, 1840, 1841, 2491, 2962, 3073
BssNI	GRCGYC	1 cut(s)	546
BssSI	CACGAG	1 cut(s)	1273
BssT1I	CCWWGG	4 cut(s)	672, 1047, 2962, 3073
Bst2BI	CACGAG	1 cut(s)	1273
Bst2UI	CCWGG	8 cut(s)	395, 1494, 1842, 1914, 2493, 3324, 3404, 3678
Bst4CI	ACNGT	8 cut(s)	180, 198, 1022, 1563, 2972, 3156, 3704, 3737
Bst6I	CTCTTC	3 cut(s)	510, 3012, 3719
BstACI	GRCGYC	1 cut(s)	546
BstAPI	GCANNNNNTGC	1 cut(s)	2741
BstC8I	GCNNGC	9 cut(s)	1204, 1423, 1433, 2312, 2572, 2588, 2729, 2733, 3316
BstDSI	CCRYGG	1 cut(s)	3073
BstEII	GGTNACC	1 cut(s)	2966
BstENI	CCTNNNNNAGG	1 cut(s)	1191
BstF5I	GGATG	8 cut(s)	1473, 2543, 2572, 2735, 3108, 3177, 3473, 3553
BstFNI	CGCG	2 cut(s)	2254, 3143
BstH2I	RGCGCY	1 cut(s)	125
BstHHI	GCGC	4 cut(s)	124, 1208, 2685, 3143
BstMAI	GTCTC	4 cut(s)	806, 847, 1070, 2198
BstMCI	CGRYCG	1 cut(s)	1590
BstNI	CCWGG	8 cut(s)	395, 1494, 1842, 1914, 2493, 3324, 3404, 3678
BstNSI	RCATGY	4 cut(s)	603, 691, 1206, 3643
BstPI	GGTNACC	1 cut(s)	2966
BstSFI	CTRYAG	6 cut(s)	176, 1139, 1433, 1786, 2354, 3602
BstUI	CGCG	2 cut(s)	2254, 3143
BstV2I	GAAGAC	2 cut(s)	336, 1589
BstX2I	RGATCY	3 cut(s)	889, 1150, 1980
BstXI	CCANNNNNNTGG	1 cut(s)	585
BstYI	RGATCY	3 cut(s)	889, 1150, 1980
Bsu15I	ATCGAT	4 cut(s)	482, 1590, 1728, 2230
BsuI	GTATCC	1 cut(s)	1173
BsuRI	GGCC	5 cut(s)	677, 1854, 2310, 2590, 3634
BsuTUI	ATCGAT	4 cut(s)	482, 1590, 1728, 2230
BtgI	CCRYGG	1 cut(s)	3073
BtgZI	GCGATG	1 cut(s)	3390
BtsCI	GGATG	8 cut(s)	1473, 2543, 2572, 2735, 3108, 3177, 3473, 3553
BtsI	GCAGTG	3 cut(s)	3363, 3599, 3611
BtsIMutI	CAGTG	8 cut(s)	1834, 2018, 2190, 2213, 3363, 3599, 3611, 3733
BveI	ACCTGC	2 cut(s)	1426, 3637
Cac8I	GCNNGC	9 cut(s)	1204, 1423, 1433, 2312, 2572, 2588, 2729, 2733, 3316
CaiI	CAGNNNCTG	1 cut(s)	3560
CciI	TCATGA	1 cut(s)	291
CfoI	GCGC	4 cut(s)	124, 1208, 2685, 3143
Cfr10I	RCCGGY	2 cut(s)	872, 1172
Cfr13I	GGNCC	6 cut(s)	535, 1250, 1322, 2137, 2180, 2506
ClaI	ATCGAT	4 cut(s)	482, 1590, 1728, 2230
CseI	GACGC	3 cut(s)	503, 535, 2345
Csp6I	GTAC	7 cut(s)	188, 205, 1672, 2057, 2173, 2460, 3049
CspAI	ACCGGT	1 cut(s)	1172
CspCI	CAANNNNNGTGG	6 cut(s)	1686, 1721, 1738, 1773, 3155, 3190
CviQI	GTAC	7 cut(s)	188, 205, 1672, 2057, 2173, 2460, 3049
DrdI	GACNNNNNNGTC	1 cut(s)	2358
DseDI	GACNNNNNNGTC	1 cut(s)	2358
EaeI	YGGCCR	1 cut(s)	2308
Eam1104I	CTCTTC	3 cut(s)	510, 3012, 3719
EarI	CTCTTC	3 cut(s)	510, 3012, 3719
EciI	GGCGGA	1 cut(s)	3115
Ecl136II	GAGCTC	1 cut(s)	1921
Eco130I	CCWWGG	4 cut(s)	672, 1047, 2962, 3073
Eco147I	AGGCCT	1 cut(s)	3634
Eco24I	GRGCYC	1 cut(s)	1923
Eco32I	GATATC	1 cut(s)	1806
Eco47I	GGWCC	6 cut(s)	535, 1250, 1322, 2137, 2180, 2506
Eco53kI	GAGCTC	1 cut(s)	1921
Eco57I	CTGAAG	2 cut(s)	1601, 2114
Eco91I	GGTNACC	1 cut(s)	2966
EcoICRI	GAGCTC	1 cut(s)	1921
EcoNI	CCTNNNNNAGG	1 cut(s)	1191
EcoO109I	RGGNCCY	3 cut(s)	1322, 2137, 2506
EcoO65I	GGTNACC	1 cut(s)	2966
EcoRI	GAATTC	3 cut(s)	901, 2975, 3038
EcoRII	CCWGG	8 cut(s)	393, 1492, 1840, 1912, 2491, 3322, 3402, 3676
EcoRV	GATATC	1 cut(s)	1806
EcoT14I	CCWWGG	4 cut(s)	672, 1047, 2962, 3073
EcoT22I	ATGCAT	1 cut(s)	1204
EcoT38I	GRGCYC	1 cut(s)	1923
ErhI	CCWWGG	4 cut(s)	672, 1047, 2962, 3073
FalI	AAGNNNNNCTT	2 cut(s)	3510, 3542
FaqI	GGGAC	6 cut(s)	667, 1308, 2292, 2492, 3165, 3464
FauI	CCCGC	1 cut(s)	2247
FauNDI	CATATG	1 cut(s)	757
FbaI	TGATCA	1 cut(s)	463
FblI	GTMKAC	3 cut(s)	1138, 3381, 3612
FokI	GGATG	8 cut(s)	1460, 2530, 2579, 2722, 3115, 3164, 3480, 3560
FriOI	GRGCYC	1 cut(s)	1923
FspAI	RTGCGCAY	1 cut(s)	2684
FspBI	CTAG	3 cut(s)	683, 2445, 3029
FspI	TGCGCA	1 cut(s)	2684
GlaI	GCGC	4 cut(s)	123, 1207, 2684, 3142
GsaI	CCCAGC	1 cut(s)	1115
GsuI	CTGGAG	4 cut(s)	1307, 1664, 2237, 3425
HaeII	RGCGCY	1 cut(s)	125
HaeIII	GGCC	5 cut(s)	677, 1854, 2310, 2590, 3634
HgaI	GACGC	3 cut(s)	503, 535, 2345
HhaI	GCGC	4 cut(s)	124, 1208, 2685, 3143
Hin1I	GRCGYC	1 cut(s)	546
Hin6I	GCGC	4 cut(s)	122, 1206, 2683, 3141
HinP1I	GCGC	4 cut(s)	122, 1206, 2683, 3141
HincII	GTYRAC	2 cut(s)	2427, 3613
HindII	GTYRAC	2 cut(s)	2427, 3613
HindIII	AAGCTT	1 cut(s)	1226
HpaI	GTTAAC	1 cut(s)	2427
HphI	GGTGA	9 cut(s)	302, 1345, 1844, 1963, 2722, 2978, 3496, 3592, 3607
Hpy166II	GTNNAC	5 cut(s)	1139, 2427, 2462, 3382, 3613
Hpy8I	GTNNAC	5 cut(s)	1139, 2427, 2462, 3382, 3613
Hpy99I	CGWCG	2 cut(s)	497, 3617
HpyAV	CCTTC	6 cut(s)	247, 720, 999, 3142, 3475, 3485
HpyCH4III	ACNGT	8 cut(s)	180, 198, 1022, 1563, 2972, 3156, 3704, 3737
HpyCH4IV	ACGT	1 cut(s)	3820
HpySE526I	ACGT	1 cut(s)	3820
Hsp92I	GRCGYC	1 cut(s)	546
HspAI	GCGC	4 cut(s)	122, 1206, 2683, 3141
KflI	GGGWCCC	1 cut(s)	2506
Kpn2I	TCCGGA	1 cut(s)	347
Ksp22I	TGATCA	1 cut(s)	463
KspAI	GTTAAC	1 cut(s)	2427
LmnI	GCTCC	7 cut(s)	608, 1288, 1496, 1513, 2009, 3075, 3406
LweI	GCATC	9 cut(s)	1124, 2309, 2348, 2557, 2609, 2706, 2744, 3093, 3186
MaeI	CTAG	3 cut(s)	683, 2445, 3029
MaeII	ACGT	1 cut(s)	3820
MbiI	CCGCTC	1 cut(s)	2025
MfeI	CAATTG	2 cut(s)	2673, 3172
MflI	RGATCY	3 cut(s)	889, 1150, 1980
MhlI	GDGCHC	2 cut(s)	1923, 3283
MlsI	TGGCCA	1 cut(s)	2310
MluNI	TGGCCA	1 cut(s)	2310
MlyI	GAGTC	5 cut(s)	808, 1962, 2200, 2490, 2545
Mox20I	TGGCCA	1 cut(s)	2310
Mph1103I	ATGCAT	1 cut(s)	1204
MroI	TCCGGA	1 cut(s)	347
MroXI	GAANNNNTTC	1 cut(s)	2127
MscI	TGGCCA	1 cut(s)	2310
MslI	CAYNNNNRTG	6 cut(s)	1040, 2286, 2621, 2984, 3297, 3604
Msp20I	TGGCCA	1 cut(s)	2310
MspA1I	CMGCKG	8 cut(s)	461, 585, 1579, 1628, 1785, 2455, 3360, 3868
MunI	CAATTG	2 cut(s)	2673, 3172
Mva1269I	GAATGC	1 cut(s)	1527
MvaI	CCWGG	8 cut(s)	395, 1494, 1842, 1914, 2493, 3324, 3404, 3678
MvnI	CGCG	2 cut(s)	2254, 3143
NciI	CCSGG	4 cut(s)	949, 1407, 2265, 3841
NcoI	CCATGG	1 cut(s)	3073
NdeI	CATATG	1 cut(s)	757
NmeAIII	GCCGAG	2 cut(s)	546, 2837
NmuCI	GTSAC	8 cut(s)	472, 1096, 1503, 1563, 2156, 2966, 3412, 3595
NsbI	TGCGCA	1 cut(s)	2684
NsiI	ATGCAT	1 cut(s)	1204
NspI	RCATGY	4 cut(s)	603, 691, 1206, 3643
OliI	CACNNNNGTG	2 cut(s)	3297, 3604
PaeI	GCATGC	1 cut(s)	1206
PagI	TCATGA	1 cut(s)	291
PaqCI	CACCTGC	1 cut(s)	3637
PasI	CCCWGGG	1 cut(s)	1841
PceI	AGGCCT	1 cut(s)	3634
PciI	ACATGT	1 cut(s)	3639
PcsI	WCGNNNNNNNCGW	1 cut(s)	3057
PctI	GAATGC	1 cut(s)	1527
PdmI	GAANNNNTTC	1 cut(s)	2127
PfeI	GAWTC	8 cut(s)	68, 850, 1334, 1621, 1720, 2112, 2293, 2372
PflFI	GACNNNGTC	1 cut(s)	3829
PflMI	CCANNNNNTGG	1 cut(s)	2215
PfoI	TCCNGGA	3 cut(s)	1492, 2263, 3402
PinAI	ACCGGT	1 cut(s)	1172
Ple19I	CGATCG	1 cut(s)	1590
PleI	GAGTC	5 cut(s)	808, 1962, 2199, 2490, 2545
PpsI	GAGTC	5 cut(s)	808, 1962, 2199, 2490, 2545
PpuMI	RGGWCCY	3 cut(s)	1322, 2137, 2506
PscI	ACATGT	1 cut(s)	3639
PshBI	ATTAAT	1 cut(s)	2784
Psp124BI	GAGCTC	1 cut(s)	1923
Psp1406I	AACGTT	1 cut(s)	3820
Psp5II	RGGWCCY	3 cut(s)	1322, 2137, 2506
Psp6I	CCWGG	8 cut(s)	393, 1492, 1840, 1912, 2491, 3322, 3402, 3676
PspEI	GGTNACC	1 cut(s)	2966
PspFI	CCCAGC	1 cut(s)	1111
PspGI	CCWGG	8 cut(s)	393, 1492, 1840, 1912, 2491, 3322, 3402, 3676
PspPI	GGNCC	6 cut(s)	535, 1250, 1322, 2137, 2180, 2506
PspPPI	RGGWCCY	3 cut(s)	1322, 2137, 2506
PsrI	GAACNNNNNNTAC	2 cut(s)	1015, 1047
PstI	CTGCAG	4 cut(s)	1437, 1790, 2358, 3606
PstNI	CAGNNNCTG	1 cut(s)	3560
PsuI	RGATCY	3 cut(s)	889, 1150, 1980
PsyI	GACNNNGTC	1 cut(s)	3829
PvuI	CGATCG	1 cut(s)	1590
PvuII	CAGCTG	6 cut(s)	461, 585, 1579, 1628, 1785, 2455
RsaI	GTAC	7 cut(s)	189, 206, 1673, 2058, 2174, 2461, 3050
RsaNI	GTAC	7 cut(s)	188, 205, 1672, 2057, 2173, 2460, 3049
RseI	CAYNNNNRTG	6 cut(s)	1040, 2286, 2621, 2984, 3297, 3604
SacI	GAGCTC	1 cut(s)	1923
SalI	GTCGAC	1 cut(s)	3611
Sau96I	GGNCC	6 cut(s)	535, 1250, 1322, 2137, 2180, 2506
SbfI	CCTGCAGG	1 cut(s)	1437
SchI	GAGTC	5 cut(s)	808, 1962, 2200, 2490, 2545
SdaI	CCTGCAGG	1 cut(s)	1437
SduI	GDGCHC	2 cut(s)	1923, 3283
SfaNI	GCATC	9 cut(s)	1124, 2309, 2348, 2557, 2609, 2706, 2744, 3093, 3186
SfcI	CTRYAG	6 cut(s)	176, 1139, 1433, 1786, 2354, 3602
SinI	GGWCC	6 cut(s)	535, 1250, 1322, 2137, 2180, 2506
SmiMI	CAYNNNNRTG	6 cut(s)	1040, 2286, 2621, 2984, 3297, 3604
SmlI	CTYRAG	7 cut(s)	1223, 1447, 1508, 1897, 2776, 2819, 3295
SmoI	CTYRAG	7 cut(s)	1223, 1447, 1508, 1897, 2776, 2819, 3295
SphI	GCATGC	1 cut(s)	1206
Sse8387I	CCTGCAGG	1 cut(s)	1437
SseBI	AGGCCT	1 cut(s)	3634
SsiI	CCGC	9 cut(s)	636, 1871, 2025, 2254, 2744, 3100, 3143, 3358, 3866
SspI	AATATT	2 cut(s)	88, 3745
SspMI	CTAG	3 cut(s)	683, 2445, 3029
SstI	GAGCTC	1 cut(s)	1923
StuI	AGGCCT	1 cut(s)	3634
StyI	CCWWGG	4 cut(s)	672, 1047, 2962, 3073
TaaI	ACNGT	8 cut(s)	180, 198, 1022, 1563, 2972, 3156, 3704, 3737
TaiI	ACGT	1 cut(s)	3823
TaqII	GACCGA	1 cut(s)	2638
TatI	WGTACW	3 cut(s)	204, 2172, 2459
TauI	GCSGC	2 cut(s)	2747, 3146
TfiI	GAWTC	8 cut(s)	68, 850, 1334, 1621, 1720, 2112, 2293, 2372
TscAI	CASTG	8 cut(s)	1834, 2025, 2190, 2220, 3370, 3606, 3611, 3740
TseFI	GTSAC	8 cut(s)	472, 1096, 1503, 1563, 2156, 2966, 3412, 3595
Tsp45I	GTSAC	8 cut(s)	472, 1096, 1503, 1563, 2156, 2966, 3412, 3595
TspGWI	ACGGA	4 cut(s)	548, 2074, 3066, 3560
TspRI	CASTG	8 cut(s)	1834, 2025, 2190, 2220, 3370, 3606, 3611, 3740
Tth111I	GACNNNGTC	1 cut(s)	3829
Van91I	CCANNNNNTGG	1 cut(s)	2215
VpaK11BI	GGWCC	6 cut(s)	535, 1250, 1322, 2137, 2180, 2506
VspI	ATTAAT	1 cut(s)	2784
XagI	CCTNNNNNAGG	1 cut(s)	1191
XapI	RAATTY	8 cut(s)	719, 901, 1648, 1847, 2436, 2975, 3038, 3807
XbaI	TCTAGA	1 cut(s)	682
XceI	RCATGY	4 cut(s)	603, 691, 1206, 3643
XmiI	GTMKAC	3 cut(s)	1138, 3381, 3612
XmnI	GAANNNNTTC	1 cut(s)	2127
XspI	CTAG	3 cut(s)	683, 2445, 3029
Zsp2I	ATGCAT	1 cut(s)	1204

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.