Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0016491

Physical Location & Seq

Genomic Coordinates Forward (+)

5294173 .. 5296774

Gene Structure 2602 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1719 bp

ATGTCGGACGGCGAAGTTGAAGCTGCCGAGCTGGTCGGCGCCGAACCCCACGACTTTGTCTCGCTTCTTTCGTCTTCCGAGAGGGACTTTCTTATTAAGGTTGAGAGCTTGAAGGGGAAAAAGCTTGGGCTGTATTTTTCTGCATCATGGTGCGGTCCGTGCAAACGATTCACCCCAGCTTTGGTTGAGGCCTACAATGAACTCTCTCCAAAAGGTGATTTTGAGATTGTATTTATCTCCGCTGATGAAGACAATGAGTCATTCGAAGAGTACTTCGCGAAGATGCCATGGCTTGCAATTCCGTTCTCTGATTCAGAGGCGCGCGATCGCGTGGATGAGTTGTTCAAAGTTAGAGGGATACCCCATCTTGTGATCCTTGGCGAAGATGGTAAAGTTTTGAGTGACGGTGGAGTTGGGATTATTCAGGAGTACGGAGCTGATGCGTACCCTTTTACGCCGGAAAAGTTGAAAGAGCTGAAAGATCAAGAAGAAGCTGCCAGAAAGGATCAGTCCTTGAAAACAATCCTGGTCACCCGCTCGCGCAACTTTGTAATTTCAAATGATGGAGAGAAGGTGCCCGTTTCTGAACTAGAAGGGAAGATAGTGGCTCTCTATTTCTCATTGTTCTCGTACGGTCCGTGCGTAGAATTTACTCCAAAACTTGTGGAGGCTCATGAGAAGCTAAAAGCAAATGGAGAGAACTTCGAGATTGTGTTGGTGCCACTTGATGATGATGAAGAATCATTCAAGCAATATTTGGAGAAAATGCCTTGGTTTTCTTTACCCATCGGGGACAAAAGTGTACAGAAGTTGGCTCGGTACTTTGAGCTCTCAACCTTGCCCACAGTGGTTGTTATTGGGGCAGATGGTAAAACACTTAATGAGAACGTTGCCGATGCCATTGACGAGCATGGGAGTCTGGCTTACCCCTTCACACCAGAGAAGTTTGCAGAGCTCGTTGAGATAGAAAAGGCAAAGGAGAAAGCTCAAACCCTGGAGTCAATTTTGATTTCCGGGGATCGAAATTTTGTCATTGGAAAAGATGGAACTCAGATCCCTGTGACAGATCTAGTGGGGAAGAACATTCTCCTGTACTTCTCAGCTCACTGGTGCCCTCCATGCCGTGCCTTTCTGCCAAAACTTGTTGAAGCCTACCACGAGATTAAGGCCAAGGACGATGCATTTGAAGTCATTTTCATCTCCAGTGACAGAGACCAAAACGCATTTGATGAGTTTTTCGCCACAATGCCATGGCTTGCTCTTCCCTTTGGTGACTCAAGGAAAGCCTTGTTGAGTCGCAAATTCAAGGTCCAGGGCATCCCCATGCTCATAGCAATCGGTCCTACTGGCCAAACCGTCACAAAAGAAGCAAGAAATCTCATCACGCATCATGGGGCAAATGCTTATCCTTTCACCGAAGAGCGAGTGAAGGAGATTGAAGCGGAATTTGAGGAGATGGCAAAGGGGTGGCCTGAGAAGTTGAAGAGTGCACTCCATGAGGAGCACGAGCTTGTGCTCTCCAAGCGTAAAGCTTTTGTGTGTGATGGGTGCGGAGAACCGGGAGGGGGGTGGTCATTCTACTGTAAGGAGTGTGATTTCGATCTCCACCCGAAGTGCGCGTTGGAGGGAGATAAAAAGACCGAAAACGGTGCCAAGCAGGAAGAAGAGGAATCCAAAGAAGGATGGGTTTGTGATGGTGGTGTTTGCACAAAGGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006935 GO:0008150 GO:0008152 GO:0009605 GO:0009636 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010035 GO:0010038 GO:0010183 GO:0010769 GO:0015036 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0040008 GO:0040011 GO:0042221 GO:0042330 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0045595 GO:0046686 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050918 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0051716 GO:0055114 GO:0060284 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0080092 GO:0097237 GO:0098754 GO:0098869 GO:1990748 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C1_2 Thioredoxin_8

Protein Analysis

573

Amino Acids

63.9

Weight (kDa)

4.83

Isoelectric Point (pI)

35.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AhpC-TSA	PF00578	32 - 132	1.9e-06	AhpC/TSA family
Redoxin	PF08534	33 - 134	1.6e-06	Redoxin
Thioredoxin	PF00085	33 - 91	5.5e-07	Thioredoxin
Thioredoxin_8	PF13905	39 - 132	1.1e-26	Thioredoxin-like
Thioredoxin_8	PF13905	199 - 291	2.5e-23	Thioredoxin-like
Redoxin	PF08534	346 - 451	3.1e-07	Redoxin
AhpC-TSA	PF00578	347 - 438	3.4e-09	AhpC/TSA family
Thioredoxin_8	PF13905	359 - 451	9.1e-27	Thioredoxin-like
Thioredoxin_2	PF13098	359 - 449	4.2e-06	Thioredoxin-like domain
C1_2	PF03107	498 - 540	1.3e-09	C1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G60420
malus_domestica	MD13G1093200.v1.1 MD16G1094200.v1.1 MD16G1094400.v1.1
prunus_persica	Prupe.1G171000_v2.0.a1 Prupe.1G171000_v2.0.a1 Prupe.1G171000_v2.0.a1 Prupe.1G252100_v2.0.a1 Prupe.1G252200_v2.0.a1 Prupe.1G252200_v2.0.a1 Prupe.1G252200_v2.0.a1 Prupe.1G252200_v2.0.a1
pyrus_communis	pycom13g08120 pycom16g08020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	5 cut(s)	38, 574, 718, 1110, 1649
AccBSI	CCGCTC	1 cut(s)	537
AccII	CGCG	6 cut(s)	278, 322, 324, 330, 541, 1619
AciI	CCGC	5 cut(s)	153, 240, 535, 1442, 1551
AclI	AACGTT	1 cut(s)	888
AclWI	GGATC	4 cut(s)	367, 513, 1026, 1048
AcoI	YGGCCR	1 cut(s)	1348
AcsI	RAATTY	4 cut(s)	647, 1024, 1301, 1445
AcyI	GRCGYC	1 cut(s)	39
AfaI	GTAC	7 cut(s)	272, 431, 446, 632, 804, 821, 1094
AfiI	CCNNNNNNNGG	2 cut(s)	181, 1565
AhdI	GACNNNNNGTC	1 cut(s)	256
AjnI	CCWGG	3 cut(s)	525, 993, 1311
AjuI	GAANNNNNNNTTGG	2 cut(s)	1604, 1636
AloI	GAACNNNNNNTCC	2 cut(s)	326, 358
Alw21I	GWGCWC	5 cut(s)	831, 957, 1492, 1506, 1518
Alw26I	GTCTC	2 cut(s)	64, 1206
Alw44I	GTGCAC	1 cut(s)	1488
AlwI	GGATC	4 cut(s)	367, 513, 1026, 1048
AoxI	GGCC	4 cut(s)	189, 1167, 1348, 1469
ApaLI	GTGCAC	1 cut(s)	1488
ApeKI	GCWGC	2 cut(s)	23, 494
ApoI	RAATTY	4 cut(s)	647, 1024, 1301, 1445
ArsI	GACNNNNNNTTYG	4 cut(s)	1166, 1198, 1293, 1325
AsiSI	GCGATCGC	1 cut(s)	328
AspLEI	GCGC	5 cut(s)	41, 322, 324, 543, 1619
AspS9I	GGNCC	4 cut(s)	155, 635, 1309, 1340
AsuC2I	CCSGG	2 cut(s)	1015, 1560
AsuHPI	GGTGA	5 cut(s)	163, 227, 523, 1283, 1405
AsuII	TTCGAA	1 cut(s)	264
AvaII	GGWCC	4 cut(s)	155, 635, 1309, 1340
BaeGI	GKGCMC	3 cut(s)	579, 1115, 1492
BalI	TGGCCA	1 cut(s)	1350
BanI	GGYRCC	5 cut(s)	38, 574, 718, 1110, 1649
BanII	GRGCYC	2 cut(s)	831, 957
BauI	CACGAG	2 cut(s)	1157, 1505
BbsI	GAAGAC	2 cut(s)	66, 255
Bbv12I	GWGCWC	5 cut(s)	831, 957, 1492, 1506, 1518
BbvI	GCAGC	2 cut(s)	10, 481
BceAI	ACGGC	2 cut(s)	25, 1107
BcgI	CGANNNNNNTGC	2 cut(s)	1631, 1665
BciT130I	CCWGG	3 cut(s)	527, 995, 1313
BciVI	GTATCC	1 cut(s)	351
BcnI	CCSGG	2 cut(s)	1015, 1560
BcoDI	GTCTC	2 cut(s)	64, 1206
BfaI	CTAG	2 cut(s)	590, 1070
BfoI	RGCGCY	1 cut(s)	42
BfuI	GTATCC	1 cut(s)	351
BglII	AGATCT	1 cut(s)	1066
BisI	GCNGC	2 cut(s)	24, 495
BlsI	GCNGC	2 cut(s)	25, 496
BmcAI	AGTACT	1 cut(s)	272
Bme1390I	CCNGG	5 cut(s)	527, 995, 1015, 1313, 1560
Bme18I	GGWCC	4 cut(s)	155, 635, 1309, 1340
BmeRI	GACNNNNNGTC	1 cut(s)	256
BmgT120I	GGNCC	4 cut(s)	155, 635, 1309, 1340
BmiI	GGNNCC	5 cut(s)	40, 576, 720, 1112, 1651
BmrFI	CCNGG	5 cut(s)	527, 995, 1015, 1313, 1560
BmsI	GCATC	7 cut(s)	152, 273, 430, 886, 1168, 1326, 1396
BpiI	GAAGAC	2 cut(s)	66, 255
BpmI	CTGGAG	2 cut(s)	1016, 1186
Bpu14I	TTCGAA	1 cut(s)	264
BpuEI	CTTGAG	1 cut(s)	1261
BpuMI	CCSGG	2 cut(s)	1015, 1560
BsaBI	GATNNNNATC	1 cut(s)	1599
BsaHI	GRCGYC	1 cut(s)	39
BsaI	GGTCTC	1 cut(s)	1206
BsaJI	CCNNGG	8 cut(s)	287, 376, 770, 993, 1014, 1170, 1250, 1312
BsaXI	ACNNNNNCTCC	2 cut(s)	558, 588
Bsc4I	CCNNNNNNNGG	2 cut(s)	181, 1565
Bse1I	ACTGG	3 cut(s)	1112, 1203, 1351
Bse8I	GATNNNNATC	1 cut(s)	1599
BseBI	CCWGG	3 cut(s)	527, 995, 1313
BseDI	CCNNGG	8 cut(s)	287, 376, 770, 993, 1014, 1170, 1250, 1312
BseGI	GGATG	3 cut(s)	340, 1317, 1688
BseJI	GATNNNNATC	1 cut(s)	1599
BseLI	CCNNNNNNNGG	2 cut(s)	181, 1565
BseMII	CTCAG	3 cut(s)	1064, 1113, 1464
BseNI	ACTGG	3 cut(s)	1112, 1203, 1351
BsePI	GCGCGC	1 cut(s)	320
BseRI	GAGGAG	2 cut(s)	1466, 1514
BseSI	GKGCMC	3 cut(s)	579, 1115, 1492
BseXI	GCAGC	2 cut(s)	10, 481
BseYI	CCCAGC	1 cut(s)	175
Bsh1236I	CGCG	6 cut(s)	278, 322, 324, 330, 541, 1619
Bsh1285I	CGRYCG	1 cut(s)	328
BshFI	GGCC	4 cut(s)	191, 1169, 1350, 1471
BshNI	GGYRCC	5 cut(s)	38, 574, 718, 1110, 1649
BsiEI	CGRYCG	1 cut(s)	328
BsiHKAI	GWGCWC	5 cut(s)	831, 957, 1492, 1506, 1518
BsiSI	CCGG	3 cut(s)	458, 1014, 1559
BsiWI	CGTACG	1 cut(s)	630
BslFI	GGGAC	2 cut(s)	98, 806
BslI	CCNNNNNNNGG	2 cut(s)	181, 1565
BsmAI	GTCTC	2 cut(s)	64, 1206
BsmFI	GGGAC	2 cut(s)	98, 806
BsnI	GGCC	4 cut(s)	191, 1169, 1350, 1471
Bso31I	GGTCTC	1 cut(s)	1206
Bsp119I	TTCGAA	1 cut(s)	264
Bsp1286I	GDGCHC	7 cut(s)	579, 831, 957, 1115, 1492, 1506, 1518
Bsp1407I	TGTACA	1 cut(s)	802
Bsp143I	GATC	8 cut(s)	325, 372, 481, 505, 1018, 1053, 1066, 1600
Bsp19I	CCATGG	2 cut(s)	287, 1250
Bsp68I	TCGCGA	1 cut(s)	278
BspACI	CCGC	5 cut(s)	153, 240, 535, 1442, 1551
BspANI	GGCC	4 cut(s)	191, 1169, 1350, 1471
BspCNI	CTCAG	3 cut(s)	1063, 1112, 1465
BspFNI	CGCG	6 cut(s)	278, 322, 324, 330, 541, 1619
BspHI	TCATGA	1 cut(s)	673
BspLI	GGNNCC	5 cut(s)	40, 576, 720, 1112, 1651
BspPI	GGATC	4 cut(s)	367, 513, 1026, 1048
BspQI	GCTCTTC	2 cut(s)	1266, 1413
BspT104I	TTCGAA	1 cut(s)	264
BspT107I	GGYRCC	5 cut(s)	38, 574, 718, 1110, 1649
BspTNI	GGTCTC	1 cut(s)	1206
BsrBI	CCGCTC	1 cut(s)	537
BsrGI	TGTACA	1 cut(s)	802
BsrI	ACTGG	3 cut(s)	1112, 1203, 1351
BssECI	CCNNGG	8 cut(s)	287, 376, 770, 993, 1014, 1170, 1250, 1312
BssHII	GCGCGC	1 cut(s)	320
BssMI	GATC	8 cut(s)	325, 372, 481, 505, 1018, 1053, 1066, 1600
BssNI	GRCGYC	1 cut(s)	39
BssSI	CACGAG	2 cut(s)	1157, 1505
BssT1I	CCWWGG	5 cut(s)	287, 376, 770, 1170, 1250
Bst2BI	CACGAG	2 cut(s)	1157, 1505
Bst2UI	CCWGG	3 cut(s)	527, 995, 1313
Bst4CI	ACNGT	6 cut(s)	407, 635, 847, 1357, 1583, 1649
Bst6I	CTCTTC	5 cut(s)	261, 1266, 1413, 1478, 1659
BstACI	GRCGYC	1 cut(s)	39
BstAUI	TGTACA	1 cut(s)	802
BstBI	TTCGAA	1 cut(s)	264
BstC8I	GCNNGC	4 cut(s)	294, 322, 539, 1257
BstDEI	CTNAG	3 cut(s)	1050, 1099, 1473
BstDSI	CCRYGG	2 cut(s)	287, 1250
BstEII	GGTNACC	1 cut(s)	529
BstF5I	GGATG	3 cut(s)	340, 1317, 1688
BstFNI	CGCG	6 cut(s)	278, 322, 324, 330, 541, 1619
BstH2I	RGCGCY	1 cut(s)	42
BstHHI	GCGC	5 cut(s)	41, 322, 324, 543, 1619
BstKTI	GATC	8 cut(s)	328, 375, 484, 508, 1021, 1056, 1069, 1603
BstMAI	GTCTC	2 cut(s)	64, 1206
BstMBI	GATC	8 cut(s)	325, 372, 481, 505, 1018, 1053, 1066, 1600
BstMCI	CGRYCG	1 cut(s)	328
BstMWI	GCNNNNNNNGC	3 cut(s)	159, 1119, 1522
BstNI	CCWGG	3 cut(s)	527, 995, 1313
BstPI	GGTNACC	1 cut(s)	529
BstSCI	CCNGG	5 cut(s)	525, 993, 1013, 1311, 1558
BstSLI	GKGCMC	3 cut(s)	579, 1115, 1492
BstUI	CGCG	6 cut(s)	278, 322, 324, 330, 541, 1619
BstV1I	GCAGC	2 cut(s)	10, 481
BstV2I	GAAGAC	2 cut(s)	66, 255
BstX2I	RGATCY	2 cut(s)	1053, 1066
BstYI	RGATCY	2 cut(s)	1053, 1066
BsuI	GTATCC	1 cut(s)	351
BsuRI	GGCC	4 cut(s)	191, 1169, 1350, 1471
BtgI	CCRYGG	2 cut(s)	287, 1250
BtsCI	GGATG	3 cut(s)	340, 1317, 1688
BtsIMutI	CAGTG	3 cut(s)	852, 1105, 1210
BtuMI	TCGCGA	1 cut(s)	278
Cac8I	GCNNGC	4 cut(s)	294, 322, 539, 1257
CciI	TCATGA	1 cut(s)	673
CfoI	GCGC	5 cut(s)	41, 322, 324, 543, 1619
Cfr13I	GGNCC	4 cut(s)	155, 635, 1309, 1340
CpoI	CGGWCCG	2 cut(s)	155, 635
Csp6I	GTAC	7 cut(s)	271, 430, 445, 631, 803, 820, 1093
CspCI	CAANNNNNGTGG	4 cut(s)	645, 680, 832, 867
CspI	CGGWCCG	2 cut(s)	155, 635
CviAII	CATG	9 cut(s)	147, 288, 674, 911, 1119, 1251, 1324, 1391, 1496
CviQI	GTAC	7 cut(s)	271, 430, 445, 631, 803, 820, 1093
DdeI	CTNAG	3 cut(s)	1050, 1099, 1473
DinI	GGCGCC	1 cut(s)	40
DpnI	GATC	8 cut(s)	327, 374, 483, 507, 1020, 1055, 1068, 1602
DpnII	GATC	8 cut(s)	325, 372, 481, 505, 1018, 1053, 1066, 1600
DriI	GACNNNNNGTC	1 cut(s)	256
EaeI	YGGCCR	1 cut(s)	1348
Eam1104I	CTCTTC	5 cut(s)	261, 1266, 1413, 1478, 1659
Eam1105I	GACNNNNNGTC	1 cut(s)	256
EarI	CTCTTC	5 cut(s)	261, 1266, 1413, 1478, 1659
Ecl136II	GAGCTC	2 cut(s)	829, 955
Eco130I	CCWWGG	5 cut(s)	287, 376, 770, 1170, 1250
Eco147I	AGGCCT	1 cut(s)	191
Eco24I	GRGCYC	2 cut(s)	831, 957
Eco31I	GGTCTC	1 cut(s)	1206
Eco47I	GGWCC	4 cut(s)	155, 635, 1309, 1340
Eco53kI	GAGCTC	2 cut(s)	829, 955
Eco91I	GGTNACC	1 cut(s)	529
EcoICRI	GAGCTC	2 cut(s)	829, 955
EcoO65I	GGTNACC	1 cut(s)	529
EcoRII	CCWGG	3 cut(s)	525, 993, 1311
EcoT14I	CCWWGG	5 cut(s)	287, 376, 770, 1170, 1250
EcoT22I	ATGCAT	1 cut(s)	1183
EcoT38I	GRGCYC	2 cut(s)	831, 957
EgeI	GGCGCC	1 cut(s)	40
EheI	GGCGCC	1 cut(s)	40
ErhI	CCWWGG	5 cut(s)	287, 376, 770, 1170, 1250
FaeI	CATG	9 cut(s)	150, 291, 677, 914, 1122, 1254, 1327, 1394, 1499
FaqI	GGGAC	2 cut(s)	98, 806
FatI	CATG	9 cut(s)	146, 287, 673, 910, 1118, 1250, 1323, 1390, 1495
FauI	CCCGC	1 cut(s)	542
Fnu4HI	GCNGC	2 cut(s)	24, 495
FokI	GGATG	3 cut(s)	347, 1304, 1695
FriOI	GRGCYC	2 cut(s)	831, 957
Fsp4HI	GCNGC	2 cut(s)	24, 495
FspBI	CTAG	2 cut(s)	590, 1070
GlaI	GCGC	5 cut(s)	40, 321, 323, 542, 1618
GluI	GCNGC	2 cut(s)	24, 495
GsaI	CCCAGC	1 cut(s)	179
GsuI	CTGGAG	2 cut(s)	1016, 1186
HaeII	RGCGCY	1 cut(s)	42
HaeIII	GGCC	4 cut(s)	191, 1169, 1350, 1471
HapII	CCGG	3 cut(s)	458, 1014, 1559
HhaI	GCGC	5 cut(s)	41, 322, 324, 543, 1619
Hin1I	GRCGYC	1 cut(s)	39
Hin1II	CATG	9 cut(s)	150, 291, 677, 914, 1122, 1254, 1327, 1394, 1499
Hin6I	GCGC	5 cut(s)	39, 320, 322, 541, 1617
HinP1I	GCGC	5 cut(s)	39, 320, 322, 541, 1617
HindIII	AAGCTT	2 cut(s)	122, 1530
HinfI	GANTC	9 cut(s)	168, 257, 311, 740, 916, 998, 1274, 1294, 1670
HpaII	CCGG	3 cut(s)	458, 1014, 1559
HphI	GGTGA	5 cut(s)	163, 227, 523, 1283, 1405
Hpy166II	GTNNAC	2 cut(s)	803, 1490
Hpy188I	TCNGA	6 cut(s)	7, 79, 310, 316, 586, 1053
Hpy188III	TCNNGA	5 cut(s)	277, 425, 485, 674, 706
Hpy8I	GTNNAC	2 cut(s)	803, 1490
HpyAV	CCTTC	6 cut(s)	106, 565, 587, 940, 1423, 1673
HpyCH4III	ACNGT	6 cut(s)	407, 635, 847, 1357, 1583, 1649
HpyCH4IV	ACGT	1 cut(s)	888
HpyCH4V	TGCA	7 cut(s)	143, 162, 296, 950, 1181, 1490, 1707
HpyF10VI	GCNNNNNNNGC	3 cut(s)	159, 1119, 1522
HpyF3I	CTNAG	3 cut(s)	1050, 1099, 1473
HpySE526I	ACGT	1 cut(s)	888
Hsp92I	GRCGYC	1 cut(s)	39
Hsp92II	CATG	9 cut(s)	150, 291, 677, 914, 1122, 1254, 1327, 1394, 1499
HspAI	GCGC	5 cut(s)	39, 320, 322, 541, 1617
KasI	GGCGCC	1 cut(s)	38
Kzo9I	GATC	8 cut(s)	325, 372, 481, 505, 1018, 1053, 1066, 1600
LguI	GCTCTTC	2 cut(s)	1266, 1413
LmnI	GCTCC	2 cut(s)	434, 1501
Lsp1109I	GCAGC	2 cut(s)	10, 481
LweI	GCATC	7 cut(s)	152, 273, 430, 886, 1168, 1326, 1396
MaeI	CTAG	2 cut(s)	590, 1070
MaeII	ACGT	1 cut(s)	888
MaeIII	GTNAC	6 cut(s)	401, 529, 1060, 1205, 1271, 1357
MalI	GATC	8 cut(s)	327, 374, 483, 507, 1020, 1055, 1068, 1602
MbiI	CCGCTC	1 cut(s)	537
MboI	GATC	8 cut(s)	325, 372, 481, 505, 1018, 1053, 1066, 1600
MflI	RGATCY	2 cut(s)	1053, 1066
MhlI	GDGCHC	7 cut(s)	579, 831, 957, 1115, 1492, 1506, 1518
MlsI	TGGCCA	1 cut(s)	1350
MluCI	AATT	7 cut(s)	297, 552, 647, 1002, 1024, 1301, 1445
MluNI	TGGCCA	1 cut(s)	1350
Mly113I	GGCGCC	1 cut(s)	39
MlyI	GAGTC	5 cut(s)	266, 925, 1007, 1268, 1303
MmeI	TCCRAC	1 cut(s)	1602
Mox20I	TGGCCA	1 cut(s)	1350
Mph1103I	ATGCAT	1 cut(s)	1183
MscI	TGGCCA	1 cut(s)	1350
MseI	TTAA	4 cut(s)	96, 879, 1164, 1717
MslI	CAYNNNNRTG	2 cut(s)	148, 1322
Msp20I	TGGCCA	1 cut(s)	1350
MspA1I	CMGCKG	1 cut(s)	242
MspI	CCGG	3 cut(s)	458, 1014, 1559
MspR9I	CCNGG	5 cut(s)	527, 995, 1015, 1313, 1560
MvaI	CCWGG	3 cut(s)	527, 995, 1313
MvnI	CGCG	6 cut(s)	278, 322, 324, 330, 541, 1619
MwoI	GCNNNNNNNGC	3 cut(s)	159, 1119, 1522
NarI	GGCGCC	1 cut(s)	39
NciI	CCSGG	2 cut(s)	1015, 1560
NcoI	CCATGG	2 cut(s)	287, 1250
NdeII	GATC	8 cut(s)	325, 372, 481, 505, 1018, 1053, 1066, 1600
NlaIII	CATG	9 cut(s)	150, 291, 677, 914, 1122, 1254, 1327, 1394, 1499
NlaIV	GGNNCC	5 cut(s)	40, 576, 720, 1112, 1651
NmeAIII	GCCGAG	1 cut(s)	52
NmuCI	GTSAC	6 cut(s)	401, 529, 1060, 1205, 1271, 1357
NruI	TCGCGA	1 cut(s)	278
NsiI	ATGCAT	1 cut(s)	1183
NspV	TTCGAA	1 cut(s)	264
PagI	TCATGA	1 cut(s)	673
PauI	GCGCGC	1 cut(s)	320
PceI	AGGCCT	1 cut(s)	191
PciSI	GCTCTTC	2 cut(s)	1266, 1413
PcsI	WCGNNNNNNNCGW	3 cut(s)	68, 635, 639
PfeI	GAWTC	4 cut(s)	168, 311, 740, 1670
Pfl23II	CGTACG	1 cut(s)	630
PflFI	GACNNNGTC	1 cut(s)	56
PkrI	GCNGC	2 cut(s)	25, 496
Ple19I	CGATCG	1 cut(s)	328
PleI	GAGTC	5 cut(s)	265, 924, 1006, 1268, 1302
PluTI	GGCGCC	1 cut(s)	42
PpsI	GAGTC	5 cut(s)	265, 924, 1006, 1268, 1302
Psp124BI	GAGCTC	2 cut(s)	831, 957
Psp1406I	AACGTT	1 cut(s)	888
Psp6I	CCWGG	3 cut(s)	525, 993, 1311
PspEI	GGTNACC	1 cut(s)	529
PspFI	CCCAGC	1 cut(s)	175
PspGI	CCWGG	3 cut(s)	525, 993, 1311
PspLI	CGTACG	1 cut(s)	630
PspN4I	GGNNCC	5 cut(s)	40, 576, 720, 1112, 1651
PspPI	GGNCC	4 cut(s)	155, 635, 1309, 1340
PsuI	RGATCY	2 cut(s)	1053, 1066
PsyI	GACNNNGTC	1 cut(s)	56
PteI	GCGCGC	1 cut(s)	320
PvuI	CGATCG	1 cut(s)	328
RgaI	GCGATCGC	1 cut(s)	328
RruI	TCGCGA	1 cut(s)	278
RsaI	GTAC	7 cut(s)	272, 431, 446, 632, 804, 821, 1094
RsaNI	GTAC	7 cut(s)	271, 430, 445, 631, 803, 820, 1093
RseI	CAYNNNNRTG	2 cut(s)	148, 1322
Rsr2I	CGGWCCG	2 cut(s)	155, 635
RsrII	CGGWCCG	2 cut(s)	155, 635
SacI	GAGCTC	2 cut(s)	831, 957
SapI	GCTCTTC	2 cut(s)	1266, 1413
SaqAI	TTAA	4 cut(s)	96, 879, 1164, 1717
SatI	GCNGC	2 cut(s)	24, 495
Sau3AI	GATC	8 cut(s)	325, 372, 481, 505, 1018, 1053, 1066, 1600
Sau96I	GGNCC	4 cut(s)	155, 635, 1309, 1340
ScaI	AGTACT	1 cut(s)	272
SchI	GAGTC	5 cut(s)	266, 925, 1007, 1268, 1303
ScrFI	CCNGG	5 cut(s)	527, 995, 1015, 1313, 1560
SduI	GDGCHC	7 cut(s)	579, 831, 957, 1115, 1492, 1506, 1518
SfaAI	GCGATCGC	1 cut(s)	328
SfaNI	GCATC	7 cut(s)	152, 273, 430, 886, 1168, 1326, 1396
SfoI	GGCGCC	1 cut(s)	40
SfuI	TTCGAA	1 cut(s)	264
SgfI	GCGATCGC	1 cut(s)	328
SinI	GGWCC	4 cut(s)	155, 635, 1309, 1340
SmiMI	CAYNNNNRTG	2 cut(s)	148, 1322
SmlI	CTYRAG	1 cut(s)	1276
SmoI	CTYRAG	1 cut(s)	1276
Sse9I	AATT	7 cut(s)	297, 552, 647, 1002, 1024, 1301, 1445
SseBI	AGGCCT	1 cut(s)	191
SsiI	CCGC	5 cut(s)	153, 240, 535, 1442, 1551
SspDI	GGCGCC	1 cut(s)	38
SspI	AATATT	1 cut(s)	755
SspMI	CTAG	2 cut(s)	590, 1070
SstI	GAGCTC	2 cut(s)	831, 957
StuI	AGGCCT	1 cut(s)	191
StyD4I	CCNGG	5 cut(s)	525, 993, 1013, 1311, 1558
StyI	CCWWGG	5 cut(s)	287, 376, 770, 1170, 1250
TaaI	ACNGT	6 cut(s)	407, 635, 847, 1357, 1583, 1649
TaiI	ACGT	1 cut(s)	891
TaqI	TCGA	4 cut(s)	264, 705, 1021, 1599
TaqII	GACCGA	2 cut(s)	1328, 1655
TasI	AATT	7 cut(s)	297, 552, 647, 1002, 1024, 1301, 1445
TatI	WGTACW	3 cut(s)	270, 802, 1092
TfiI	GAWTC	4 cut(s)	168, 311, 740, 1670
Tru1I	TTAA	4 cut(s)	96, 879, 1164, 1717
Tru9I	TTAA	4 cut(s)	96, 879, 1164, 1717
TscAI	CASTG	3 cut(s)	852, 1112, 1210
TseFI	GTSAC	6 cut(s)	401, 529, 1060, 1205, 1271, 1357
TseI	GCWGC	2 cut(s)	23, 494
Tsp45I	GTSAC	6 cut(s)	401, 529, 1060, 1205, 1271, 1357
TspDTI	ATGAA	4 cut(s)	213, 261, 750, 1186
TspGWI	ACGGA	4 cut(s)	147, 291, 447, 627
TspRI	CASTG	3 cut(s)	852, 1112, 1210
Tth111I	GACNNNGTC	1 cut(s)	56
VneI	GTGCAC	1 cut(s)	1488
VpaK11BI	GGWCC	4 cut(s)	155, 635, 1309, 1340
XapI	RAATTY	4 cut(s)	647, 1024, 1301, 1445
XspI	CTAG	2 cut(s)	590, 1070
ZrmI	AGTACT	1 cut(s)	272
Zsp2I	ATGCAT	1 cut(s)	1183

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.