pycom16g08570

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007325
Physical Location & Seq
Forward (+)
5702445 .. 5703629
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
ATGTCAATCTGTCCCTCTCAAAATTCATTGCAATTGCTGAGGCAATACCAGAGTTTGCAAGGCTTGACCATGGATCATCTCTCCAGAGCAATTGACATTTACCTCAAGGCACATCCAGACCTAAACAAAAGTGAAAGGAAGAGGCTTTGCAGAATTCTAGACTGCAAAAAGCTCTCAATGGAGGTCTGCATGCATGCTGCACAAAATGAGCTGCTCCCACTAAGAGTGGTGGTCCAAGTTCTTTTCTTTGAGCAAGCTAAAGCCGCCATGGCTGGCGGCAAAGTGACCGAGCTGCCTAGCAACATTAGGGCTCTGCTAGCCTCGCATAACATTGATCCGTCAAAGCCTCCGCCCTCTCTAAGCAACGCTAGTATTGTTCCGGCAGAGGATCAATGGAGCGTTTCAGGCCTGAAATCGCCAAAGTCAAAGACGTCAACTTTACGAATGAAACTGGATGAAGACGATGATTTGGACGACAATGATATGCATCCTGATGGACTTGGGAGAAATTCAAAGTTCAAATCTCTTTGTTCACTTCCTGCTAAACCCAAAAGAATGTTCAGTAAGTTGTGGTCCATAAACAGAACTAGCAGTGAAAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

202

Amino Acids

22.48

Weight (kDa)

9.22

Isoelectric Point (pI)

58.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 23 - 63 4.3e-17 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 434
AciI CCGC 3 cut(s) 264, 276, 350
AclWI GGATC 3 cut(s) 81, 329, 396
AcsI RAATTY 3 cut(s) 22, 153, 508
AcyI GRCGYC 1 cut(s) 431
AgsI TTSAA 2 cut(s) 513, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 542, 574
AluBI AGCT 4 cut(s) 172, 211, 257, 292
AluI AGCT 4 cut(s) 172, 211, 257, 292
AlwI GGATC 3 cut(s) 81, 329, 396
AoxI GGCC 1 cut(s) 406
ApeKI GCWGC 3 cut(s) 197, 211, 292
ApoI RAATTY 3 cut(s) 22, 153, 508
AspS9I GGNCC 2 cut(s) 232, 573
AsuNHI GCTAGC 1 cut(s) 316
AvaII GGWCC 2 cut(s) 232, 573
BanII GRGCYC 1 cut(s) 313
BbsI GAAGAC 1 cut(s) 465
BbvCI CCTCAGC 1 cut(s) 38
BbvI GCAGC 3 cut(s) 184, 198, 279
BccI CCATC 1 cut(s) 488
BfaI CTAG 5 cut(s) 158, 297, 317, 369, 588
BglI GCCNNNNNGGC 1 cut(s) 269
BisI GCNGC 5 cut(s) 198, 212, 264, 277, 293
BlsI GCNGC 5 cut(s) 199, 213, 265, 278, 294
Bme18I GGWCC 2 cut(s) 232, 573
BmgT120I GGNCC 2 cut(s) 232, 573
BmsI GCATC 1 cut(s) 496
BmtI GCTAGC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 67
Bpu10I CCTNAGC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 89
BsaBI GATNNNNATC 1 cut(s) 486
BsaHI GRCGYC 1 cut(s) 431
BsaJI CCNNGG 2 cut(s) 69, 267
Bse1I ACTGG 1 cut(s) 456
Bse3DI GCAATG 1 cut(s) 26
Bse8I GATNNNNATC 1 cut(s) 486
BseDI CCNNGG 2 cut(s) 69, 267
BseGI GGATG 3 cut(s) 112, 460, 487
BseJI GATNNNNATC 1 cut(s) 486
BseMI GCAATG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 1 cut(s) 456
BseXI GCAGC 3 cut(s) 184, 198, 279
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 1 cut(s) 408
BsiSI CCGG 1 cut(s) 380
BsnI GGCC 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 3 cut(s) 73, 334, 388
Bsp19I CCATGG 2 cut(s) 69, 267
BspACI CCGC 3 cut(s) 264, 276, 350
BspANI GGCC 1 cut(s) 408
BspCNI CTCAG 1 cut(s) 30
BspOI GCTAGC 1 cut(s) 320
BspPI GGATC 3 cut(s) 81, 329, 396
BsrDI GCAATG 1 cut(s) 26
BsrI ACTGG 1 cut(s) 456
BssECI CCNNGG 2 cut(s) 69, 267
BssMI GATC 3 cut(s) 73, 334, 388
BssNI GRCGYC 1 cut(s) 431
BssT1I CCWWGG 2 cut(s) 69, 267
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 1 cut(s) 431
BstC8I GCNNGC 5 cut(s) 191, 195, 255, 274, 318
BstDEI CTNAG 3 cut(s) 38, 221, 359
BstDSI CCRYGG 2 cut(s) 69, 267
BstF5I GGATG 3 cut(s) 112, 460, 487
BstKTI GATC 3 cut(s) 76, 337, 391
BstMBI GATC 3 cut(s) 73, 334, 388
BstMWI GCNNNNNNNGC 5 cut(s) 263, 269, 317, 322, 405
BstNSI RCATGY 2 cut(s) 193, 197
BstV1I GCAGC 3 cut(s) 184, 198, 279
BstV2I GAAGAC 1 cut(s) 465
BsuRI GGCC 1 cut(s) 408
BtgI CCRYGG 2 cut(s) 69, 267
BtsCI GGATG 3 cut(s) 112, 460, 487
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 1 cut(s) 598
Cac8I GCNNGC 5 cut(s) 191, 195, 255, 274, 318
Cfr13I GGNCC 2 cut(s) 232, 573
CviAII CATG 4 cut(s) 70, 190, 194, 268
DdeI CTNAG 3 cut(s) 38, 221, 359
DpnI GATC 3 cut(s) 75, 336, 390
DpnII GATC 3 cut(s) 73, 334, 388
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
EciI GGCGGA 1 cut(s) 339
Eco130I CCWWGG 2 cut(s) 69, 267
Eco147I AGGCCT 1 cut(s) 408
Eco24I GRGCYC 1 cut(s) 313
Eco47I GGWCC 2 cut(s) 232, 573
EcoRI GAATTC 1 cut(s) 153
EcoT14I CCWWGG 2 cut(s) 69, 267
EcoT22I ATGCAT 2 cut(s) 195, 489
EcoT38I GRGCYC 1 cut(s) 313
ErhI CCWWGG 2 cut(s) 69, 267
FaeI CATG 4 cut(s) 73, 193, 197, 271
FaiI YATR 7 cut(s) 71, 191, 195, 269, 327, 485, 578
FatI CATG 4 cut(s) 69, 189, 193, 267
Fnu4HI GCNGC 5 cut(s) 198, 212, 264, 277, 293
FokI GGATG 3 cut(s) 99, 467, 474
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 5 cut(s) 198, 212, 264, 277, 293
FspBI CTAG 5 cut(s) 158, 297, 317, 369, 588
GluI GCNGC 5 cut(s) 198, 212, 264, 277, 293
GsuI CTGGAG 1 cut(s) 67
HaeIII GGCC 1 cut(s) 408
HapII CCGG 1 cut(s) 380
Hin1I GRCGYC 1 cut(s) 431
Hin1II CATG 4 cut(s) 73, 193, 197, 271
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HpaII CCGG 1 cut(s) 380
Hpy166II GTNNAC 2 cut(s) 435, 533
Hpy188III TCNNGA 4 cut(s) 84, 116, 158, 491
Hpy8I GTNNAC 2 cut(s) 435, 533
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 8 cut(s) 31, 58, 150, 165, 189, 193, 200, 487
HpyF10VI GCNNNNNNNGC 5 cut(s) 263, 269, 317, 322, 405
HpyF3I CTNAG 3 cut(s) 38, 221, 359
HpySE526I ACGT 1 cut(s) 431
Hsp92I GRCGYC 1 cut(s) 431
Hsp92II CATG 4 cut(s) 73, 193, 197, 271
Kzo9I GATC 3 cut(s) 73, 334, 388
LmnI GCTCC 2 cut(s) 219, 396
Lsp1109I GCAGC 3 cut(s) 184, 198, 279
LweI GCATC 1 cut(s) 496
MaeI CTAG 5 cut(s) 158, 297, 317, 369, 588
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 1 cut(s) 283
MalI GATC 3 cut(s) 75, 336, 390
MboI GATC 3 cut(s) 73, 334, 388
MboII GAAGA 2 cut(s) 151, 470
MfeI CAATTG 2 cut(s) 32, 90
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 5 cut(s) 22, 32, 90, 153, 508
MnlI CCTC 9 cut(s) 25, 33, 113, 135, 175, 331, 357, 364, 379
Mph1103I ATGCAT 2 cut(s) 195, 489
MslI CAYNNNNRTG 1 cut(s) 492
MspI CCGG 1 cut(s) 380
MunI CAATTG 2 cut(s) 32, 90
MwoI GCNNNNNNNGC 5 cut(s) 263, 269, 317, 322, 405
NcoI CCATGG 2 cut(s) 69, 267
NdeII GATC 3 cut(s) 73, 334, 388
NheI GCTAGC 1 cut(s) 316
NlaIII CATG 4 cut(s) 73, 193, 197, 271
NmuCI GTSAC 1 cut(s) 283
NsiI ATGCAT 2 cut(s) 195, 489
NspI RCATGY 2 cut(s) 193, 197
PaeI GCATGC 2 cut(s) 193, 197
PceI AGGCCT 1 cut(s) 408
PkrI GCNGC 5 cut(s) 199, 213, 265, 278, 294
PspPI GGNCC 2 cut(s) 232, 573
RseI CAYNNNNRTG 1 cut(s) 492
SatI GCNGC 5 cut(s) 198, 212, 264, 277, 293
Sau3AI GATC 3 cut(s) 73, 334, 388
Sau96I GGNCC 2 cut(s) 232, 573
SduI GDGCHC 1 cut(s) 313
SetI ASST 8 cut(s) 105, 123, 174, 186, 213, 259, 294, 434
SfaNI GCATC 1 cut(s) 496
SinI GGWCC 2 cut(s) 232, 573
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SphI GCATGC 2 cut(s) 193, 197
Sse9I AATT 5 cut(s) 22, 32, 90, 153, 508
SseBI AGGCCT 1 cut(s) 408
SsiI CCGC 3 cut(s) 264, 276, 350
SspMI CTAG 5 cut(s) 158, 297, 317, 369, 588
StuI AGGCCT 1 cut(s) 408
StyI CCWWGG 2 cut(s) 69, 267
TaiI ACGT 1 cut(s) 434
TaqII GACCGA 1 cut(s) 302
TasI AATT 5 cut(s) 22, 32, 90, 153, 508
TauI GCSGC 2 cut(s) 266, 279
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 3 cut(s) 197, 211, 292
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 3 cut(s) 15, 461, 471
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 1 cut(s) 598
VpaK11BI GGWCC 2 cut(s) 232, 573
XapI RAATTY 3 cut(s) 22, 153, 508
XbaI TCTAGA 1 cut(s) 157
XceI RCATGY 2 cut(s) 193, 197
XspI CTAG 5 cut(s) 158, 297, 317, 369, 588
ZraI GACGTC 1 cut(s) 432
Zsp2I ATGCAT 2 cut(s) 195, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.