pycom16g14030

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014383
Physical Location & Seq
Forward (+)
10044687 .. 10046361
1675 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1557 bp
ATGGTTGAAGCAGCAGAGCCATCCCAAAAGTGCCCGACAATGCCAGAGGTGGTGGAAGAACTTCACAGAATGACAAATATTGGCTTCCCAATTGCGGCCATGAGTTTAGTGGGTTACCTCAAAAACATGGTCCTAGTTGTATGCATGGGAAGGCTCGGAAGTCTTGAGCTAGCAGGTGGGGCTTTAGCCATTGGCTTCACCAACATCACTGGCTACTCAGTCCTCTCTGGCTTGGCCATGGGTATGGAGCCTCTATGCAGCCAAGCTTTTGGTTCACAGAACTTCTCCATAGCTTTCCACACTTTACAAAGAACCATTCTCTTATTGCTTCTCACTTCTCTTCCTATTGCTTTGCTTTGGGCCAATCTTGAACCTCTCATGCTCCTCCTCCATCAAAACCAAGATATAACGAAGATTGCTAGCTTGTATTGCCAATTTGCCATCCCTGATCTTCTTGCCAATAGCCTTCTTCATCCCATACGTATTTTTTTACGTAGTCAAAGCACAACATGGCCATTGATGTGGTCCACTTTACTAGCAATCATTCTACACATTCCTCTCACAATCTTCTTAACCTTCAATCTTTCCCTTGGGATCAAAGGAATAGCAATTTCCACTTCTCTTACCAATTTTATCACTCTATTTTTTCTTTTGGGCTATGTGGTCTATGCCCATGTCGCAAAAAAATCTATTTATTGTTTGGTAACGGACCAGTGTGAACCTACTACTAAGTATGAAACAACACAATTATTGTCCCAGCCATTATTACCCAAAAAAATAGTACCACTTTCGTTGAGGAAAACGCTAGGAGCTGACGAATGGAGTATCCTAATTCGACTTGCTTTCCAAAGTTGCTTAGGAGTGTGCTTAGAATGGTGGTGGTATGAGTTCATGACAATTCTAACCGGTTACCTTCAAAAACCTCACATTGCCCTAGCAGCATCAGCCATAGTGATACAAACCACATCTCTCATGTACACATTACCAGCGGCACTAAGTGCATCAGTGTCTACACGAGTAGGCAACGAGCTAGGGGCAGGCCAACCCGAAAAGGCACGTTTAGCGGCAGTGGTGGCAGTGGGGATGGCACTAGTGAGCTCATTGTTGGGCTTGTCATTGACCACTCTAGGGAGAGGAGCATGGGGAAGAATCTTCACAAATGATAGTGAGGTTTTGGAGCTAACAATGAGTGTGCTACCAATTATTGGACTATGTGAGATTGCTAATTGTCCACAAACTACAAGTTGTGGGATTTTGAGAGGAAGTGCTAGGCCAGGCATTGGGGCATGGATTAACTTTTACTCATTTTACATGGTGGGTACGCCTGTGGCAATAGTCTTGACGTTTGTGTGGAGGTTAGGGTTTAAAGGGCTTTGTTATGGACTTCTAGCAGCTCAAATTACATGTGTGGTGTCCATATTAACAGTGGTTCATAAGACAAATTGGGAGAGAGAATGTCTCAAGACGAAAGAACTTGTAGGAAAGAATAATGACAGAATGGCTGCATTTGCACATGCAGATGAAACAGTCAAATGTGAAGAGGGTGTTGCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

519

Amino Acids

56.53

Weight (kDa)

8.12

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 30 - 188 8.3e-33 MatE
MatE PF01554 285 - 445 1.1e-31 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49130
fragaria_vesca FvH4_4g18550
malus_domestica MD13G1166100.v1.1 MD16G1165600.v1.1
prunus_persica Prupe.1G132600_v2.0.a1
pyrus_communis pycom16g14030
rosa_chinensis RchiOBHm_Chr4g0423121
rosa_laevigata RLG00000007582
rosa_multiflora Rmu_sc0000744.1_g000050 Rmu_sc0003669.1_g000032
rosa_roxburghii Rroxscaffold_5G00365200
rosa_rugosa Rorug04G0184100
rosa_samantha Rh4AG244000 Rh4BG249100 Rh4CG258300 Rh4DG243600
rosa_wichuraiana Rw4G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 164
AccB7I CCANNNNNTGG 2 cut(s) 1205, 1280
AccI GTMKAC 1 cut(s) 1010
AciI CCGC 3 cut(s) 95, 989, 1064
AclWI GGATC 1 cut(s) 602
AcoI YGGCCR 3 cut(s) 96, 234, 512
AdeI CACNNNGTG 1 cut(s) 998
AfaI GTAC 3 cut(s) 783, 977, 1321
AfiI CCNNNNNNNGG 4 cut(s) 94, 1128, 1205, 1280
AflIII ACRYGT 1 cut(s) 1403
AgeI ACCGGT 1 cut(s) 905
AgsI TTSAA 4 cut(s) 8, 371, 580, 917
AhlI ACTAGT 1 cut(s) 1090
AjnI CCWGG 1 cut(s) 1273
AluBI AGCT 9 cut(s) 169, 266, 293, 423, 812, 1030, 1098, 1180, 1394
AluI AGCT 9 cut(s) 169, 266, 293, 423, 812, 1030, 1098, 1180, 1394
Alw21I GWGCWC 1 cut(s) 1100
Alw26I GTCTC 1 cut(s) 1463
AlwI GGATC 1 cut(s) 602
AoxI GGCC 6 cut(s) 96, 234, 360, 512, 1039, 1271
ApeKI GCWGC 5 cut(s) 11, 258, 938, 1391, 1502
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
AsiGI ACCGGT 1 cut(s) 905
Asp700I GAANNNNTTC 1 cut(s) 60
AspS9I GGNCC 4 cut(s) 130, 360, 525, 709
AsuHPI GGTGA 1 cut(s) 190
AsuNHI GCTAGC 2 cut(s) 169, 419
AvaII GGWCC 3 cut(s) 130, 525, 709
BaeGI GKGCMC 1 cut(s) 35
BaeI ACNNNNGTAYC 2 cut(s) 1311, 1344
BalI TGGCCA 2 cut(s) 236, 514
BanII GRGCYC 1 cut(s) 1100
BauI CACGAG 1 cut(s) 1014
Bbv12I GWGCWC 1 cut(s) 1100
BbvI GCAGC 5 cut(s) 23, 270, 950, 1403, 1489
BccI CCATC 4 cut(s) 28, 399, 449, 1078
BciT130I CCWGG 1 cut(s) 1275
BciVI GTATCC 1 cut(s) 836
BcoDI GTCTC 1 cut(s) 1463
BcuI ACTAGT 1 cut(s) 1090
BfuAI ACCTGC 1 cut(s) 164
BfuI GTATCC 1 cut(s) 836
BisI GCNGC 8 cut(s) 12, 96, 259, 939, 990, 1065, 1392, 1503
BlsI GCNGC 8 cut(s) 13, 97, 260, 940, 991, 1066, 1393, 1504
Bme1390I CCNGG 1 cut(s) 1275
Bme18I GGWCC 3 cut(s) 130, 525, 709
BmgT120I GGNCC 4 cut(s) 130, 360, 525, 709
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 1275
BmsI GCATC 2 cut(s) 950, 1010
BmtI GCTAGC 2 cut(s) 173, 423
BplI GAGNNNNNCTC 2 cut(s) 1443, 1475
Bpu10I CCTNAGC 1 cut(s) 856
BpuEI CTTGAG 2 cut(s) 185, 1445
BsaAI YACGTR 2 cut(s) 482, 494
BsaJI CCNNGG 2 cut(s) 237, 589
BsaWI WCCGGW 1 cut(s) 905
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 1128, 1205, 1280
Bse118I RCCGGY 1 cut(s) 905
Bse1I ACTGG 2 cut(s) 214, 712
Bse3DI GCAATG 1 cut(s) 927
BseBI CCWGG 1 cut(s) 1275
BseDI CCNNGG 2 cut(s) 237, 589
BseGI GGATG 4 cut(s) 20, 441, 472, 1089
BseLI CCNNNNNNNGG 4 cut(s) 94, 1128, 1205, 1280
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 214, 712
BseRI GAGGAG 3 cut(s) 374, 377, 1149
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 5 cut(s) 23, 270, 950, 1403, 1489
BseYI CCCAGC 1 cut(s) 756
BshFI GGCC 6 cut(s) 98, 236, 362, 514, 1041, 1273
BshTI ACCGGT 1 cut(s) 905
BsiHKAI GWGCWC 1 cut(s) 1100
BsiSI CCGG 1 cut(s) 906
BslFI GGGAC 1 cut(s) 739
BslI CCNNNNNNNGG 4 cut(s) 94, 1128, 1205, 1280
BsmAI GTCTC 1 cut(s) 1463
BsmFI GGGAC 1 cut(s) 739
BsnI GGCC 6 cut(s) 98, 236, 362, 514, 1041, 1273
Bsp1286I GDGCHC 2 cut(s) 35, 1100
Bsp1407I TGTACA 1 cut(s) 975
Bsp143I GATC 2 cut(s) 448, 594
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 3 cut(s) 95, 989, 1064
BspANI GGCC 6 cut(s) 98, 236, 362, 514, 1041, 1273
BspCNI CTCAG 1 cut(s) 230
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 1 cut(s) 249
BspMI ACCTGC 1 cut(s) 164
BspOI GCTAGC 2 cut(s) 173, 423
BspPI GGATC 1 cut(s) 602
BsrDI GCAATG 1 cut(s) 927
BsrFI RCCGGY 1 cut(s) 905
BsrGI TGTACA 1 cut(s) 975
BsrI ACTGG 2 cut(s) 214, 712
BssAI RCCGGY 1 cut(s) 905
BssECI CCNNGG 2 cut(s) 237, 589
BssMI GATC 2 cut(s) 448, 594
BssSI CACGAG 1 cut(s) 1014
BssT1I CCWWGG 2 cut(s) 237, 589
Bst2BI CACGAG 1 cut(s) 1014
Bst2UI CCWGG 1 cut(s) 1275
Bst4CI ACNGT 2 cut(s) 1426, 1528
Bst6I CTCTTC 2 cut(s) 345, 1533
BstAPI GCANNNNNTGC 1 cut(s) 998
BstAUI TGTACA 1 cut(s) 975
BstBAI YACGTR 2 cut(s) 482, 494
BstC8I GCNNGC 3 cut(s) 171, 421, 1039
BstDEI CTNAG 5 cut(s) 217, 729, 856, 868, 995
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 2 cut(s) 113, 908
BstF5I GGATG 4 cut(s) 20, 441, 472, 1089
BstKTI GATC 2 cut(s) 451, 597
BstMAI GTCTC 1 cut(s) 1463
BstMBI GATC 2 cut(s) 448, 594
BstMWI GCNNNNNNNGC 9 cut(s) 179, 429, 677, 938, 944, 998, 1061, 1073, 1508
BstNI CCWGG 1 cut(s) 1275
BstNSI RCATGY 2 cut(s) 1407, 1517
BstPI GGTNACC 2 cut(s) 113, 908
BstSCI CCNGG 1 cut(s) 1273
BstSLI GKGCMC 1 cut(s) 35
BstSNI TACGTA 2 cut(s) 482, 494
BstV1I GCAGC 5 cut(s) 23, 270, 950, 1403, 1489
BstXI CCANNNNNNTGG 3 cut(s) 244, 269, 522
BsuI GTATCC 1 cut(s) 836
BsuRI GGCC 6 cut(s) 98, 236, 362, 514, 1041, 1273
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 4 cut(s) 20, 441, 472, 1089
BtsI GCAGTG 2 cut(s) 1074, 1083
BtsIMutI CAGTG 6 cut(s) 207, 719, 1011, 1074, 1083, 1431
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 3 cut(s) 171, 421, 1039
CciI TCATGA 1 cut(s) 891
Cfr10I RCCGGY 1 cut(s) 905
Cfr13I GGNCC 4 cut(s) 130, 360, 525, 709
Csp6I GTAC 3 cut(s) 782, 976, 1320
CspAI ACCGGT 1 cut(s) 905
CspCI CAANNNNNGTGG 2 cut(s) 774, 809
CviQI GTAC 3 cut(s) 782, 976, 1320
DdeI CTNAG 5 cut(s) 217, 729, 856, 868, 995
DpnI GATC 2 cut(s) 450, 596
DpnII GATC 2 cut(s) 448, 594
DraI TTTAAA 1 cut(s) 1366
DraIII CACNNNGTG 1 cut(s) 998
EaeI YGGCCR 3 cut(s) 96, 234, 512
Eam1104I CTCTTC 2 cut(s) 345, 1533
EarI CTCTTC 2 cut(s) 345, 1533
Ecl136II GAGCTC 1 cut(s) 1098
Eco105I TACGTA 2 cut(s) 482, 494
Eco130I CCWWGG 2 cut(s) 237, 589
Eco24I GRGCYC 1 cut(s) 1100
Eco47I GGWCC 3 cut(s) 130, 525, 709
Eco53kI GAGCTC 1 cut(s) 1098
Eco91I GGTNACC 2 cut(s) 113, 908
EcoICRI GAGCTC 1 cut(s) 1098
EcoO65I GGTNACC 2 cut(s) 113, 908
EcoRII CCWGG 1 cut(s) 1273
EcoT14I CCWWGG 2 cut(s) 237, 589
EcoT22I ATGCAT 1 cut(s) 146
EcoT38I GRGCYC 1 cut(s) 1100
ErhI CCWWGG 2 cut(s) 237, 589
FaqI GGGAC 1 cut(s) 739
FblI GTMKAC 1 cut(s) 1010
Fnu4HI GCNGC 8 cut(s) 12, 96, 259, 939, 990, 1065, 1392, 1503
FokI GGATG 4 cut(s) 7, 428, 459, 1096
FriOI GRGCYC 1 cut(s) 1100
Fsp4HI GCNGC 8 cut(s) 12, 96, 259, 939, 990, 1065, 1392, 1503
GluI GCNGC 8 cut(s) 12, 96, 259, 939, 990, 1065, 1392, 1503
GsaI CCCAGC 1 cut(s) 760
HaeIII GGCC 6 cut(s) 98, 236, 362, 514, 1041, 1273
HapII CCGG 1 cut(s) 906
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 1 cut(s) 1149
HpaII CCGG 1 cut(s) 906
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 6 cut(s) 275, 528, 719, 978, 1011, 1232
Hpy188I TCNGA 1 cut(s) 158
Hpy188III TCNNGA 5 cut(s) 164, 368, 892, 1339, 1462
Hpy8I GTNNAC 6 cut(s) 275, 528, 719, 978, 1011, 1232
HpyAV CCTTC 4 cut(s) 144, 476, 586, 923
HpyCH4III ACNGT 2 cut(s) 1426, 1528
HpyCH4IV ACGT 4 cut(s) 481, 493, 1057, 1343
HpyCH4V TGCA 7 cut(s) 144, 258, 1001, 1505, 1511, 1517, 1550
HpyF10VI GCNNNNNNNGC 9 cut(s) 179, 429, 677, 938, 944, 998, 1061, 1073, 1508
HpyF3I CTNAG 5 cut(s) 217, 729, 856, 868, 995
HpySE526I ACGT 4 cut(s) 481, 493, 1057, 1343
Kzo9I GATC 2 cut(s) 448, 594
LmnI GCTCC 5 cut(s) 247, 387, 809, 1136, 1177
Lsp1109I GCAGC 5 cut(s) 23, 270, 950, 1403, 1489
LweI GCATC 2 cut(s) 950, 1010
MaeII ACGT 4 cut(s) 481, 493, 1057, 1343
MaeIII GTNAC 3 cut(s) 113, 703, 908
MalI GATC 2 cut(s) 450, 596
MboI GATC 2 cut(s) 448, 594
MboII GAAGA 9 cut(s) 68, 332, 424, 443, 461, 559, 1144, 1158, 1550
MfeI CAATTG 1 cut(s) 90
MhlI GDGCHC 2 cut(s) 35, 1100
MlsI TGGCCA 2 cut(s) 236, 514
MluNI TGGCCA 2 cut(s) 236, 514
Mox20I TGGCCA 2 cut(s) 236, 514
Mph1103I ATGCAT 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 60
MscI TGGCCA 2 cut(s) 236, 514
MseI TTAA 4 cut(s) 572, 1293, 1365, 1421
MslI CAYNNNNRTG 3 cut(s) 242, 520, 1518
Msp20I TGGCCA 2 cut(s) 236, 514
MspA1I CMGCKG 1 cut(s) 989
MspI CCGG 1 cut(s) 906
MspR9I CCNGG 1 cut(s) 1275
MunI CAATTG 1 cut(s) 90
MvaI CCWGG 1 cut(s) 1275
MwoI GCNNNNNNNGC 9 cut(s) 179, 429, 677, 938, 944, 998, 1061, 1073, 1508
NcoI CCATGG 1 cut(s) 237
NdeII GATC 2 cut(s) 448, 594
NheI GCTAGC 2 cut(s) 169, 419
NlaIV GGNNCC 1 cut(s) 249
NsiI ATGCAT 1 cut(s) 146
NspI RCATGY 2 cut(s) 1407, 1517
PagI TCATGA 1 cut(s) 891
PaqCI CACCTGC 1 cut(s) 164
PciI ACATGT 1 cut(s) 1403
PdmI GAANNNNTTC 1 cut(s) 60
PfeI GAWTC 1 cut(s) 1149
PflMI CCANNNNNTGG 2 cut(s) 1205, 1280
PinAI ACCGGT 1 cut(s) 905
PkrI GCNGC 8 cut(s) 13, 97, 260, 940, 991, 1066, 1393, 1504
Ppu21I YACGTR 2 cut(s) 482, 494
PscI ACATGT 1 cut(s) 1403
Psp124BI GAGCTC 1 cut(s) 1100
Psp6I CCWGG 1 cut(s) 1273
PspEI GGTNACC 2 cut(s) 113, 908
PspFI CCCAGC 1 cut(s) 756
PspGI CCWGG 1 cut(s) 1273
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 4 cut(s) 130, 360, 525, 709
RsaI GTAC 3 cut(s) 783, 977, 1321
RsaNI GTAC 3 cut(s) 782, 976, 1320
RseI CAYNNNNRTG 3 cut(s) 242, 520, 1518
SacI GAGCTC 1 cut(s) 1100
SaqAI TTAA 4 cut(s) 572, 1293, 1365, 1421
SatI GCNGC 8 cut(s) 12, 96, 259, 939, 990, 1065, 1392, 1503
Sau3AI GATC 2 cut(s) 448, 594
Sau96I GGNCC 4 cut(s) 130, 360, 525, 709
ScrFI CCNGG 1 cut(s) 1275
SduI GDGCHC 2 cut(s) 35, 1100
SfaNI GCATC 2 cut(s) 950, 1010
SinI GGWCC 3 cut(s) 130, 525, 709
SmiMI CAYNNNNRTG 3 cut(s) 242, 520, 1518
SmlI CTYRAG 2 cut(s) 164, 1460
SmoI CTYRAG 2 cut(s) 164, 1460
SnaBI TACGTA 2 cut(s) 482, 494
SpeI ACTAGT 1 cut(s) 1090
SsiI CCGC 3 cut(s) 95, 989, 1064
SspI AATATT 1 cut(s) 79
SstI GAGCTC 1 cut(s) 1100
StyD4I CCNGG 1 cut(s) 1273
StyI CCWWGG 2 cut(s) 237, 589
TaaI ACNGT 2 cut(s) 1426, 1528
TaiI ACGT 4 cut(s) 484, 496, 1060, 1346
TaqI TCGA 1 cut(s) 835
TatI WGTACW 1 cut(s) 975
TauI GCSGC 3 cut(s) 98, 992, 1067
TfiI GAWTC 1 cut(s) 1149
Tru1I TTAA 4 cut(s) 572, 1293, 1365, 1421
Tru9I TTAA 4 cut(s) 572, 1293, 1365, 1421
TscAI CASTG 6 cut(s) 214, 719, 1011, 1074, 1083, 1431
TseI GCWGC 5 cut(s) 11, 258, 938, 1391, 1502
TspDTI ATGAA 5 cut(s) 461, 750, 880, 1421, 1536
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 6 cut(s) 214, 719, 1011, 1074, 1083, 1431
Van91I CCANNNNNTGG 2 cut(s) 1205, 1280
VpaK11BI GGWCC 3 cut(s) 130, 525, 709
XceI RCATGY 2 cut(s) 1407, 1517
XcmI CCANNNNNNNNNTGG 2 cut(s) 106, 1423
XmiI GTMKAC 1 cut(s) 1010
XmnI GAANNNNTTC 1 cut(s) 60
Zsp2I ATGCAT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.