pycom17g22740

Catalyzes the second two steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylmonomethylethanolamine (PMME) to phosphatidyldimethylethanolamine (PDME) and of PDME to phosphatidylcholine (PC)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0014527
Physical Location & Seq
Reverse (-)
21141901 .. 21148699
6799 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGGGGATAGCAGCAGCAATAGGAGTGCTTTCACCCTTCCCATTCTACTATTGGCTATGGAGTTACCCACAAACATGGGTAGAGATGTGTGGGAAGGGGCGTGACCCATGCAAAGTGATGGCCTATGTGTCTCATTTCCTGAAGCTGATTCAGTTCCTTTCTCTTTTCTCCGTCTCTACCTTCTCTTGGCCTCCCCCTCTTTACTTCTGGCCCCTCATTGCCTTTGGCCAGTTCCTCAACTTCAGGGTCTATCAGTTGCTCGGTGAATCTGGTACGTATTATGGAGTACGTTTTGGGAAGAACATTCCCTGGGTGACAGAATTTCCATTTGGGTACGTAAAAGATCCTCAGTATGTGGGAAGCATTTTAAGTCTTTTTGCATGCGTATCGTGGGTACCATTTCAGTACATTTCGTTGTGGACGTTGGGTTATGTTGCAATGATATATTTGGAGTCGAAGGAAGATCCTGCAACTCGCGCAAAACCACGCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko01110 map00564 map01100 map01110
Pfam Domains
Protein Families
PEMT

Protein Analysis

165

Amino Acids

18.99

Weight (kDa)

8.94

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEMT PF04191 72 - 158 3.9e-12 Phospholipid methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80860 AT1G80860
fragaria_vesca FvH4_6g28630
malus_domestica MD17G1223400.v1.1 MD17G1223600.v1.1
prunus_persica Prupe.3G098300_v2.0.a1
pyrus_communis pycom17g22740
rosa_chinensis RchiOBHm_Chr2g0132951
rosa_laevigata RLG00000019296
rosa_multiflora Rmu_sc0007314.1_g000002
rosa_roxburghii Rroxscaffold_2G00111590
rosa_rugosa Rorug02G0308700
rosa_samantha Rh2AG361200 Rh2BG367000 Rh2CG344400 Rh2DG384000
rosa_wichuraiana Rw2G029370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 394
AccB1I GGYRCC 1 cut(s) 394
AccII CGCG 1 cut(s) 477
AclWI GGATC 2 cut(s) 338, 458
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 1 cut(s) 320
AcuI CTGAAG 2 cut(s) 161, 226
AfaI GTAC 5 cut(s) 274, 288, 335, 396, 407
AfiI CCNNNNNNNGG 1 cut(s) 186
AjnI CCWGG 1 cut(s) 308
AjuI GAANNNNNNNTTGG 2 cut(s) 312, 344
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
Alw26I GTCTC 2 cut(s) 135, 178
AlwI GGATC 2 cut(s) 338, 458
AoxI GGCC 4 cut(s) 120, 188, 209, 226
ApeKI GCWGC 2 cut(s) 11, 14
ApoI RAATTY 1 cut(s) 320
Asp718I GGTACC 1 cut(s) 394
AspLEI GCGC 1 cut(s) 479
AspS9I GGNCC 1 cut(s) 210
AsuHPI GGTGA 3 cut(s) 24, 275, 325
BalI TGGCCA 1 cut(s) 228
BanI GGYRCC 1 cut(s) 394
BbvI GCAGC 2 cut(s) 23, 26
BccI CCATC 1 cut(s) 112
BcgI CGANNNNNNTGC 2 cut(s) 369, 403
BciT130I CCWGG 1 cut(s) 310
BcoDI GTCTC 2 cut(s) 135, 178
BisI GCNGC 2 cut(s) 12, 15
BlsI GCNGC 2 cut(s) 13, 16
Bme1390I CCNGG 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 210
BmiI GGNNCC 2 cut(s) 212, 396
BmrFI CCNGG 1 cut(s) 310
BsaAI YACGTR 2 cut(s) 276, 337
BsaJI CCNNGG 2 cut(s) 308, 309
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 2 cut(s) 216, 444
BseBI CCWGG 1 cut(s) 310
BseDI CCNNGG 2 cut(s) 308, 309
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMI GCAATG 2 cut(s) 216, 444
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 2 cut(s) 23, 26
Bsh1236I CGCG 1 cut(s) 477
BshFI GGCC 4 cut(s) 122, 190, 211, 228
BshNI GGYRCC 1 cut(s) 394
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 2 cut(s) 135, 178
BsmBI CGTCTC 1 cut(s) 178
BsnI GGCC 4 cut(s) 122, 190, 211, 228
Bsp143I GATC 2 cut(s) 343, 463
BspANI GGCC 4 cut(s) 122, 190, 211, 228
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 1 cut(s) 477
BspLI GGNNCC 2 cut(s) 212, 396
BspPI GGATC 2 cut(s) 338, 458
BspT107I GGYRCC 1 cut(s) 394
BsrDI GCAATG 2 cut(s) 216, 444
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 2 cut(s) 308, 309
BssMI GATC 2 cut(s) 343, 463
Bst2UI CCWGG 1 cut(s) 310
BstBAI YACGTR 2 cut(s) 276, 337
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 1 cut(s) 348
BstFNI CGCG 1 cut(s) 477
BstHHI GCGC 1 cut(s) 479
BstKTI GATC 2 cut(s) 346, 466
BstMAI GTCTC 2 cut(s) 135, 178
BstMBI GATC 2 cut(s) 343, 463
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstNI CCWGG 1 cut(s) 310
BstNSI RCATGY 1 cut(s) 384
BstSCI CCNGG 1 cut(s) 308
BstSNI TACGTA 2 cut(s) 276, 337
BstUI CGCG 1 cut(s) 477
BstV1I GCAGC 2 cut(s) 23, 26
BstX2I RGATCY 2 cut(s) 343, 463
BstXI CCANNNNNNTGG 1 cut(s) 75
BstYI RGATCY 2 cut(s) 343, 463
BsuRI GGCC 4 cut(s) 122, 190, 211, 228
Cac8I GCNNGC 1 cut(s) 382
CfoI GCGC 1 cut(s) 479
Cfr13I GGNCC 1 cut(s) 210
Csp6I GTAC 5 cut(s) 273, 287, 334, 395, 406
CviAII CATG 3 cut(s) 75, 108, 381
CviJI RGCY 6 cut(s) 55, 122, 145, 190, 211, 228
CviKI_1 RGCY 6 cut(s) 55, 122, 145, 190, 211, 228
CviQI GTAC 5 cut(s) 273, 287, 334, 395, 406
DdeI CTNAG 1 cut(s) 348
DpnI GATC 2 cut(s) 345, 465
DpnII GATC 2 cut(s) 343, 463
EaeI YGGCCR 1 cut(s) 226
Eco105I TACGTA 2 cut(s) 276, 337
Eco57I CTGAAG 2 cut(s) 161, 226
EcoRII CCWGG 1 cut(s) 308
Esp3I CGTCTC 1 cut(s) 178
FaeI CATG 3 cut(s) 78, 111, 384
FatI CATG 3 cut(s) 74, 107, 380
Fnu4HI GCNGC 2 cut(s) 12, 15
Fsp4HI GCNGC 2 cut(s) 12, 15
GlaI GCGC 1 cut(s) 478
GluI GCNGC 2 cut(s) 12, 15
HaeIII GGCC 4 cut(s) 122, 190, 211, 228
HhaI GCGC 1 cut(s) 479
Hin1II CATG 3 cut(s) 78, 111, 384
Hin6I GCGC 1 cut(s) 477
HinP1I GCGC 1 cut(s) 477
HinfI GANTC 3 cut(s) 148, 266, 452
HphI GGTGA 3 cut(s) 24, 275, 325
Hpy166II GTNNAC 1 cut(s) 420
Hpy188III TCNNGA 1 cut(s) 139
Hpy8I GTNNAC 1 cut(s) 420
HpyAV CCTTC 4 cut(s) 46, 88, 190, 451
HpyCH4IV ACGT 4 cut(s) 275, 289, 336, 422
HpyCH4V TGCA 4 cut(s) 111, 380, 437, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpyF3I CTNAG 1 cut(s) 348
HpySE526I ACGT 4 cut(s) 275, 289, 336, 422
Hsp92II CATG 3 cut(s) 78, 111, 384
HspAI GCGC 1 cut(s) 477
KpnI GGTACC 1 cut(s) 398
Kzo9I GATC 2 cut(s) 343, 463
LpnPI CCDG 8 cut(s) 152, 193, 229, 242, 255, 295, 322, 480
Lsp1109I GCAGC 2 cut(s) 23, 26
MaeII ACGT 4 cut(s) 275, 289, 336, 422
MaeIII GTNAC 3 cut(s) 62, 101, 313
MalI GATC 2 cut(s) 345, 465
MboI GATC 2 cut(s) 343, 463
MboII GAAGA 2 cut(s) 310, 473
MflI RGATCY 2 cut(s) 343, 463
MlsI TGGCCA 1 cut(s) 228
MluCI AATT 1 cut(s) 320
MluNI TGGCCA 1 cut(s) 228
MlyI GAGTC 1 cut(s) 461
MnlI CCTC 5 cut(s) 201, 207, 224, 245, 357
Mox20I TGGCCA 1 cut(s) 228
MscI TGGCCA 1 cut(s) 228
MseI TTAA 1 cut(s) 368
MslI CAYNNNNRTG 1 cut(s) 73
Msp20I TGGCCA 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 310
MvaI CCWGG 1 cut(s) 310
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 1 cut(s) 476
NdeII GATC 2 cut(s) 343, 463
NlaIII CATG 3 cut(s) 78, 111, 384
NlaIV GGNNCC 2 cut(s) 212, 396
NmuCI GTSAC 2 cut(s) 101, 313
NspI RCATGY 1 cut(s) 384
PaeI GCATGC 1 cut(s) 384
PasI CCCWGGG 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 419
PfeI GAWTC 2 cut(s) 148, 266
PkrI GCNGC 2 cut(s) 13, 16
PleI GAGTC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 460
Ppu21I YACGTR 2 cut(s) 276, 337
Psp6I CCWGG 1 cut(s) 308
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 2 cut(s) 212, 396
PspPI GGNCC 1 cut(s) 210
PsuI RGATCY 2 cut(s) 343, 463
RsaI GTAC 5 cut(s) 274, 288, 335, 396, 407
RsaNI GTAC 5 cut(s) 273, 287, 334, 395, 406
RseI CAYNNNNRTG 1 cut(s) 73
SaqAI TTAA 1 cut(s) 368
SatI GCNGC 2 cut(s) 12, 15
Sau3AI GATC 2 cut(s) 343, 463
Sau96I GGNCC 1 cut(s) 210
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 1 cut(s) 310
SetI ASST 6 cut(s) 147, 182, 278, 292, 339, 425
SmiMI CAYNNNNRTG 1 cut(s) 73
SnaBI TACGTA 2 cut(s) 276, 337
SphI GCATGC 1 cut(s) 384
Sse9I AATT 1 cut(s) 320
StyD4I CCNGG 1 cut(s) 308
TaiI ACGT 4 cut(s) 278, 292, 339, 425
TaqI TCGA 1 cut(s) 455
TasI AATT 1 cut(s) 320
TatI WGTACW 1 cut(s) 405
TfiI GAWTC 2 cut(s) 148, 266
Tru1I TTAA 1 cut(s) 368
Tru9I TTAA 1 cut(s) 368
TseFI GTSAC 2 cut(s) 101, 313
TseI GCWGC 2 cut(s) 11, 14
Tsp45I GTSAC 2 cut(s) 101, 313
TspGWI ACGGA 1 cut(s) 160
XapI RAATTY 1 cut(s) 320
XceI RCATGY 1 cut(s) 384
XcmI CCANNNNNNNNNTGG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.