pycom461g00080

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0019146
Physical Location & Seq
Reverse (-)
42111 .. 42582
472 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom461g00080.2

Sequence Viewer

Length: 408 bp
ATGATGACAACATCCCCCGCAATTCAACTTGAGGGTTACCGGTCAAGGGGGGAGTATTGGCAGGGGGTTAAAACCGCCCTGGATGAAGCGACCTCTCAGGGGGAGTATAACCGCCTACTGGATTTCGAAAATAGGGATCTCCGGATTCGGGAGCAGAGGCATTCGTGCTATTGCCTTTTCGAACAGGTGCTTTCTGAGCATCCGTCCTTGGCGGAAAGAGCCCCCTACGATCCTAAAGAAGTCTTTTTTGATTTCGTTTCCGAGGCGCGGAACGGGCTGGACGCCCATCAGGAGTGGAGCCCTCGGGAAAAGGACTTCCGAGAACTAGAATTTTTAAGTGGACTTCAAAAAGGCCTCCGAGAAAGGGGCGCCAACTCACTAGTTTTAGCTCGGATTCTGCGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

15.81

Weight (kDa)

5.45

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07695 ATMG01280
pyrus_communis pycom461g00080
rosa_chinensis RchiOBHm_MTg0498241
rosa_laevigata RLG00000000359 RLG00000000373 RLG00000000389
rosa_roxburghii Rroxscaffold_7G00202780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 368
AccII CGCG 1 cut(s) 268
AccIII TCCGGA 1 cut(s) 141
AciI CCGC 6 cut(s) 18, 75, 112, 212, 268, 400
AclWI GGATC 2 cut(s) 144, 224
AcsI RAATTY 1 cut(s) 329
AcyI GRCGYC 2 cut(s) 282, 369
AfiI CCNNNNNNNGG 6 cut(s) 46, 99, 118, 148, 267, 364
AgeI ACCGGT 1 cut(s) 39
AgsI TTSAA 2 cut(s) 26, 347
AhlI ACTAGT 1 cut(s) 379
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 1 cut(s) 389
AluI AGCT 1 cut(s) 389
AlwI GGATC 2 cut(s) 144, 224
Ama87I CYCGRG 1 cut(s) 303
Aor13HI TCCGGA 1 cut(s) 141
AoxI GGCC 1 cut(s) 352
ApoI RAATTY 1 cut(s) 329
AsiGI ACCGGT 1 cut(s) 39
AspLEI GCGC 2 cut(s) 268, 371
AsuII TTCGAA 2 cut(s) 126, 180
AvaI CYCGRG 1 cut(s) 303
BanI GGYRCC 1 cut(s) 368
BanII GRGCYC 2 cut(s) 223, 302
BccI CCATC 1 cut(s) 294
BciT130I CCWGG 1 cut(s) 80
BcuI ACTAGT 1 cut(s) 379
BfaI CTAG 2 cut(s) 326, 380
BfoI RGCGCY 1 cut(s) 372
BisI GCNGC 1 cut(s) 401
BlsI GCNGC 1 cut(s) 402
Bme1390I CCNGG 1 cut(s) 80
BmeT110I CYCGRG 1 cut(s) 303
BmiI GGNNCC 2 cut(s) 299, 370
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 208
Bpu14I TTCGAA 2 cut(s) 126, 180
BpuEI CTTGAG 1 cut(s) 50
BsaHI GRCGYC 2 cut(s) 282, 369
BsaJI CCNNGG 4 cut(s) 78, 207, 261, 302
BsaWI WCCGGW 2 cut(s) 39, 141
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 99, 118, 148, 267, 364
Bse118I RCCGGY 1 cut(s) 39
Bse1I ACTGG 1 cut(s) 123
BseAI TCCGGA 1 cut(s) 141
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 4 cut(s) 78, 207, 261, 302
BseGI GGATG 3 cut(s) 11, 88, 199
BseLI CCNNNNNNNGG 6 cut(s) 46, 99, 118, 148, 267, 364
BseMII CTCAG 2 cut(s) 110, 186
BseNI ACTGG 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 268
BshFI GGCC 1 cut(s) 354
BshNI GGYRCC 1 cut(s) 368
BshTI ACCGGT 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 2 cut(s) 40, 142
BslI CCNNNNNNNGG 6 cut(s) 46, 99, 118, 148, 267, 364
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 1 cut(s) 354
BsoBI CYCGRG 1 cut(s) 303
Bsp119I TTCGAA 2 cut(s) 126, 180
Bsp1286I GDGCHC 2 cut(s) 223, 302
Bsp13I TCCGGA 1 cut(s) 141
Bsp143I GATC 2 cut(s) 136, 229
BspACI CCGC 6 cut(s) 18, 75, 112, 212, 268, 400
BspANI GGCC 1 cut(s) 354
BspCNI CTCAG 2 cut(s) 109, 187
BspEI TCCGGA 1 cut(s) 141
BspFNI CGCG 1 cut(s) 268
BspLI GGNNCC 2 cut(s) 299, 370
BspPI GGATC 2 cut(s) 144, 224
BspT104I TTCGAA 2 cut(s) 126, 180
BspT107I GGYRCC 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 39
BsrI ACTGG 1 cut(s) 123
BssAI RCCGGY 1 cut(s) 39
BssECI CCNNGG 4 cut(s) 78, 207, 261, 302
BssMI GATC 2 cut(s) 136, 229
BssNI GRCGYC 2 cut(s) 282, 369
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 80
BstACI GRCGYC 2 cut(s) 282, 369
BstBI TTCGAA 2 cut(s) 126, 180
BstDEI CTNAG 2 cut(s) 96, 195
BstEII GGTNACC 1 cut(s) 35
BstF5I GGATG 3 cut(s) 11, 88, 199
BstFNI CGCG 1 cut(s) 268
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 2 cut(s) 268, 371
BstKTI GATC 2 cut(s) 139, 232
BstMBI GATC 2 cut(s) 136, 229
BstMWI GCNNNNNNNGC 3 cut(s) 196, 218, 274
BstNI CCWGG 1 cut(s) 80
BstPI GGTNACC 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 78
BstUI CGCG 1 cut(s) 268
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BsuRI GGCC 1 cut(s) 354
BtsCI GGATG 3 cut(s) 11, 88, 199
CfoI GCGC 2 cut(s) 268, 371
Cfr10I RCCGGY 1 cut(s) 39
CseI GACGC 1 cut(s) 290
CspAI ACCGGT 1 cut(s) 39
CviJI RGCY 6 cut(s) 221, 277, 300, 354, 389, 403
CviKI_1 RGCY 6 cut(s) 221, 277, 300, 354, 389, 403
DdeI CTNAG 2 cut(s) 96, 195
DinI GGCGCC 1 cut(s) 370
DpnI GATC 2 cut(s) 138, 231
DpnII GATC 2 cut(s) 136, 229
EciI GGCGGA 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 207
Eco147I AGGCCT 1 cut(s) 354
Eco24I GRGCYC 2 cut(s) 223, 302
Eco88I CYCGRG 1 cut(s) 303
Eco91I GGTNACC 1 cut(s) 35
EcoO65I GGTNACC 1 cut(s) 35
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 207
EcoT38I GRGCYC 2 cut(s) 223, 302
EgeI GGCGCC 1 cut(s) 370
EheI GGCGCC 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 207
FaiI YATR 2 cut(s) 108, 406
FauI CCCGC 1 cut(s) 25
Fnu4HI GCNGC 1 cut(s) 401
FokI GGATG 2 cut(s) 95, 186
FriOI GRGCYC 2 cut(s) 223, 302
Fsp4HI GCNGC 1 cut(s) 401
FspBI CTAG 2 cut(s) 326, 380
GlaI GCGC 2 cut(s) 267, 370
GluI GCNGC 1 cut(s) 401
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 1 cut(s) 354
HapII CCGG 2 cut(s) 40, 142
HgaI GACGC 1 cut(s) 290
HhaI GCGC 2 cut(s) 268, 371
Hin1I GRCGYC 2 cut(s) 282, 369
Hin6I GCGC 2 cut(s) 266, 369
HinP1I GCGC 2 cut(s) 266, 369
HinfI GANTC 2 cut(s) 145, 394
HpaII CCGG 2 cut(s) 40, 142
Hpy166II GTNNAC 1 cut(s) 341
Hpy188I TCNGA 5 cut(s) 196, 262, 320, 359, 393
Hpy188III TCNNGA 4 cut(s) 142, 149, 290, 305
Hpy8I GTNNAC 1 cut(s) 341
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 218, 274
HpyF3I CTNAG 2 cut(s) 96, 195
Hsp92I GRCGYC 2 cut(s) 282, 369
HspAI GCGC 2 cut(s) 266, 369
KasI GGCGCC 1 cut(s) 368
Kpn2I TCCGGA 1 cut(s) 141
Kzo9I GATC 2 cut(s) 136, 229
LmnI GCTCC 2 cut(s) 151, 297
LweI GCATC 1 cut(s) 208
MaeI CTAG 2 cut(s) 326, 380
MaeIII GTNAC 1 cut(s) 35
MalI GATC 2 cut(s) 138, 231
MboI GATC 2 cut(s) 136, 229
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 2 cut(s) 223, 302
MluCI AATT 2 cut(s) 21, 329
Mly113I GGCGCC 1 cut(s) 369
MnlI CCTC 6 cut(s) 25, 103, 150, 256, 312, 365
MroI TCCGGA 1 cut(s) 141
MseI TTAA 2 cut(s) 69, 335
MspI CCGG 2 cut(s) 40, 142
MspR9I CCNGG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 1 cut(s) 80
MvnI CGCG 1 cut(s) 268
MwoI GCNNNNNNNGC 3 cut(s) 196, 218, 274
NarI GGCGCC 1 cut(s) 369
NdeII GATC 2 cut(s) 136, 229
NlaIV GGNNCC 2 cut(s) 299, 370
NspV TTCGAA 2 cut(s) 126, 180
PceI AGGCCT 1 cut(s) 354
PctI GAATGC 1 cut(s) 160
PfeI GAWTC 2 cut(s) 145, 394
PinAI ACCGGT 1 cut(s) 39
PkrI GCNGC 1 cut(s) 402
PluTI GGCGCC 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 78
PspEI GGTNACC 1 cut(s) 35
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 2 cut(s) 299, 370
PsuI RGATCY 1 cut(s) 136
SaqAI TTAA 2 cut(s) 69, 335
SatI GCNGC 1 cut(s) 401
Sau3AI GATC 2 cut(s) 136, 229
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 2 cut(s) 223, 302
SetI ASST 3 cut(s) 95, 189, 391
SfaNI GCATC 1 cut(s) 208
SfoI GGCGCC 1 cut(s) 370
SfuI TTCGAA 2 cut(s) 126, 180
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SpeI ACTAGT 1 cut(s) 379
Sse9I AATT 2 cut(s) 21, 329
SseBI AGGCCT 1 cut(s) 354
SsiI CCGC 6 cut(s) 18, 75, 112, 212, 268, 400
SspDI GGCGCC 1 cut(s) 368
SspMI CTAG 2 cut(s) 326, 380
StuI AGGCCT 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 78
StyI CCWWGG 1 cut(s) 207
TaqI TCGA 2 cut(s) 126, 180
TasI AATT 2 cut(s) 21, 329
TauI GCSGC 1 cut(s) 403
TfiI GAWTC 2 cut(s) 145, 394
Tru1I TTAA 2 cut(s) 69, 335
Tru9I TTAA 2 cut(s) 69, 335
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 1 cut(s) 192
XapI RAATTY 1 cut(s) 329
XspI CTAG 2 cut(s) 326, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.