pycom5153684g00150

Catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012527_4311101_to_5153684
OG0015233
Physical Location & Seq
Forward (+)
297216 .. 297806
591 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 591 bp
ATGGAGATGGAGACGGCTGATGATGAGAAAAATCTAGTGGAAATCTTAGAGGAGGAGATCCAAATTGACATGGCCAAATATATCAACTACGTGAGTGCCCCACAGGCGGGTGCCATTGCCACATTTTCGGGTACCACGCGCGACACTTTTGAGGGCAAAACAGTCTTGGAGCTCAGATATGAAGCATATGTTCCCATGGCAATACGGTGCCTAAAATCCATTTGTTCATCTGCTAGATCATCCTGGAATCTCCTCTCCATTGCAGTTGCCCACCGCCTGGGCCCCGTTCCAGTTGGTCAAACAAGTGTTTTCATTGCAGTGTCATCTATTCATCGAGTTGATGCATTGGATGCTTGTAAATTTGTGATTGATGAGATAAAGGCATCAGTTCCAATATGGAAAAAGGAGGTTTATGCTAATGGAGAGGTTTGGAAGGAGAATTCAGAGTTCCTAGAGCGGAGGTTGGATCTCGGGAAAAAAGATGACGTGTATTATGAGAAGCAAGTCGAAGCTGATTCCGAAAGGCATAGCAGGAAGAGCTGTTGTGGGGCTAAGGTCAAGGTCACTGAGGAAGGAGACGGAAAGTCGTAG

Protein Analysis

197

Amino Acids

21.93

Weight (kDa)

5.15

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MoaE PF02391 21 - 128 2.1e-37 MoaE protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43760 AT2G43760 AT2G43760
fragaria_vesca FvH4_1g18980
prunus_persica Prupe.4G269700_v2.0.a1 Prupe.4G269700_v2.0.a1 Prupe.4G269700_v2.0.a1
pyrus_communis pycom5153684g00150
rosa_chinensis RchiOBHm_Chr2g0110001
rosa_roxburghii Rroxscaffold_2G00133790
rosa_rugosa Rorug02G0165100
rosa_samantha Rh2AG216600 Rh2BG226600 Rh2CG218800 Rh2DG222200
rosa_wichuraiana Rw2G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 131
AccB1I GGYRCC 3 cut(s) 110, 131, 207
AccB7I CCANNNNNTGG 1 cut(s) 277
AccBSI CCGCTC 1 cut(s) 457
AccII CGCG 2 cut(s) 139, 141
AciI CCGC 3 cut(s) 107, 274, 457
AclWI GGATC 2 cut(s) 52, 474
AcoI YGGCCR 1 cut(s) 72
AcsI RAATTY 2 cut(s) 359, 439
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 3 cut(s) 106, 107, 277
AflIII ACRYGT 1 cut(s) 486
AhdI GACNNNNNGTC 1 cut(s) 583
AjiI CACGTC 1 cut(s) 487
AjnI CCWGG 2 cut(s) 242, 276
AluBI AGCT 3 cut(s) 172, 512, 540
AluI AGCT 3 cut(s) 172, 512, 540
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 2 cut(s) 5, 570
AlwI GGATC 2 cut(s) 52, 474
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 2 cut(s) 72, 280
ApaI GGGCCC 1 cut(s) 284
ApoI RAATTY 2 cut(s) 359, 439
Asp718I GGTACC 1 cut(s) 131
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 2 cut(s) 280, 281
AvaI CYCGRG 1 cut(s) 470
BaeGI GKGCMC 2 cut(s) 100, 284
BalI TGGCCA 1 cut(s) 74
BanI GGYRCC 3 cut(s) 110, 131, 207
BanII GRGCYC 2 cut(s) 174, 284
Bbv12I GWGCWC 1 cut(s) 174
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 2 cut(s) 244, 278
BcoDI GTCTC 2 cut(s) 5, 570
BfaI CTAG 3 cut(s) 35, 234, 452
BglI GCCNNNNNGGC 1 cut(s) 104
Bme1390I CCNGG 2 cut(s) 244, 278
BmeRI GACNNNNNGTC 1 cut(s) 583
BmeT110I CYCGRG 1 cut(s) 470
BmgBI CACGTC 1 cut(s) 487
BmgT120I GGNCC 2 cut(s) 280, 281
BmiI GGNNCC 5 cut(s) 112, 133, 209, 282, 283
BmrFI CCNGG 2 cut(s) 244, 278
BmsI GCATC 3 cut(s) 331, 340, 392
Bpu10I CCTNAGC 1 cut(s) 552
BsaAI YACGTR 1 cut(s) 91
BsaJI CCNNGG 2 cut(s) 195, 277
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 107, 277
Bse1I ACTGG 1 cut(s) 290
Bse3DI GCAATG 3 cut(s) 114, 258, 312
BseBI CCWGG 2 cut(s) 244, 278
BseDI CCNNGG 2 cut(s) 195, 277
BseGI GGATG 2 cut(s) 239, 355
BseLI CCNNNNNNNGG 3 cut(s) 106, 107, 277
BseMI GCAATG 3 cut(s) 114, 258, 312
BseMII CTCAG 2 cut(s) 187, 558
BseNI ACTGG 1 cut(s) 290
BseRI GAGGAG 3 cut(s) 65, 68, 242
BseSI GKGCMC 2 cut(s) 100, 284
Bsh1236I CGCG 2 cut(s) 139, 141
BshFI GGCC 2 cut(s) 74, 282
BshNI GGYRCC 3 cut(s) 110, 131, 207
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 470
BslI CCNNNNNNNGG 3 cut(s) 106, 107, 277
BsmAI GTCTC 2 cut(s) 5, 570
BsmBI CGTCTC 2 cut(s) 5, 570
BsnI GGCC 2 cut(s) 74, 282
BsoBI CYCGRG 1 cut(s) 470
Bsp120I GGGCCC 1 cut(s) 280
Bsp1286I GDGCHC 3 cut(s) 100, 174, 284
Bsp143I GATC 3 cut(s) 57, 236, 466
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 3 cut(s) 107, 274, 457
BspANI GGCC 2 cut(s) 74, 282
BspCNI CTCAG 2 cut(s) 186, 559
BspFNI CGCG 2 cut(s) 139, 141
BspLI GGNNCC 5 cut(s) 112, 133, 209, 282, 283
BspPI GGATC 2 cut(s) 52, 474
BspQI GCTCTTC 1 cut(s) 530
BspT107I GGYRCC 3 cut(s) 110, 131, 207
BsrBI CCGCTC 1 cut(s) 457
BsrDI GCAATG 3 cut(s) 114, 258, 312
BsrI ACTGG 1 cut(s) 290
BssECI CCNNGG 2 cut(s) 195, 277
BssMI GATC 3 cut(s) 57, 236, 466
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 2 cut(s) 244, 278
Bst4CI ACNGT 2 cut(s) 163, 207
Bst6I CTCTTC 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 350
BstBAI YACGTR 1 cut(s) 91
BstDEI CTNAG 4 cut(s) 46, 173, 552, 567
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 2 cut(s) 239, 355
BstFNI CGCG 2 cut(s) 139, 141
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 3 cut(s) 60, 239, 469
BstMAI GTCTC 2 cut(s) 5, 570
BstMBI GATC 3 cut(s) 57, 236, 466
BstMWI GCNNNNNNNGC 3 cut(s) 104, 350, 537
BstNI CCWGG 2 cut(s) 244, 278
BstSCI CCNGG 2 cut(s) 242, 276
BstSLI GKGCMC 2 cut(s) 100, 284
BstUI CGCG 2 cut(s) 139, 141
BstX2I RGATCY 2 cut(s) 57, 466
BstYI RGATCY 2 cut(s) 57, 466
BsuRI GGCC 2 cut(s) 74, 282
BtgI CCRYGG 1 cut(s) 195
BtrI CACGTC 1 cut(s) 487
BtsCI GGATG 2 cut(s) 239, 355
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 2 cut(s) 324, 564
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 2 cut(s) 280, 281
Csp6I GTAC 1 cut(s) 132
CviAII CATG 2 cut(s) 70, 196
CviJI RGCY 7 cut(s) 17, 74, 172, 282, 512, 540, 551
CviKI_1 RGCY 7 cut(s) 17, 74, 172, 282, 512, 540, 551
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 4 cut(s) 46, 173, 552, 567
DpnI GATC 3 cut(s) 59, 238, 468
DpnII GATC 3 cut(s) 57, 236, 466
DriI GACNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 72
Eam1104I CTCTTC 1 cut(s) 530
Eam1105I GACNNNNNGTC 1 cut(s) 583
EarI CTCTTC 1 cut(s) 530
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 2 cut(s) 174, 284
Eco53kI GAGCTC 1 cut(s) 172
Eco88I CYCGRG 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 281
EcoRI GAATTC 1 cut(s) 439
EcoRII CCWGG 2 cut(s) 242, 276
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 346
EcoT38I GRGCYC 2 cut(s) 174, 284
ErhI CCWWGG 1 cut(s) 195
Esp3I CGTCTC 2 cut(s) 5, 570
FaeI CATG 2 cut(s) 73, 199
FatI CATG 2 cut(s) 69, 195
FauI CCCGC 1 cut(s) 100
FauNDI CATATG 1 cut(s) 187
FokI GGATG 2 cut(s) 226, 362
FriOI GRGCYC 2 cut(s) 174, 284
FspBI CTAG 3 cut(s) 35, 234, 452
GlaI GCGC 1 cut(s) 140
HaeIII GGCC 2 cut(s) 74, 282
HhaI GCGC 1 cut(s) 141
Hin1II CATG 2 cut(s) 73, 199
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HinfI GANTC 2 cut(s) 247, 515
Hpy188I TCNGA 3 cut(s) 176, 445, 520
Hpy188III TCNNGA 1 cut(s) 472
HpyAV CCTTC 2 cut(s) 427, 566
HpyCH4III ACNGT 2 cut(s) 163, 207
HpyCH4IV ACGT 2 cut(s) 90, 486
HpyCH4V TGCA 3 cut(s) 263, 317, 344
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 350, 537
HpyF3I CTNAG 4 cut(s) 46, 173, 552, 567
HpySE526I ACGT 2 cut(s) 90, 486
Hsp92II CATG 2 cut(s) 73, 199
HspAI GCGC 1 cut(s) 139
KpnI GGTACC 1 cut(s) 135
Kzo9I GATC 3 cut(s) 57, 236, 466
LguI GCTCTTC 1 cut(s) 530
LmnI GCTCC 1 cut(s) 169
LpnPI CCDG 7 cut(s) 89, 229, 256, 263, 290, 303, 517
LweI GCATC 3 cut(s) 331, 340, 392
MaeI CTAG 3 cut(s) 35, 234, 452
MaeII ACGT 2 cut(s) 90, 486
MaeIII GTNAC 1 cut(s) 562
MalI GATC 3 cut(s) 59, 238, 468
MbiI CCGCTC 1 cut(s) 457
MboI GATC 3 cut(s) 57, 236, 466
MboII GAAGA 1 cut(s) 547
MflI RGATCY 2 cut(s) 57, 466
MhlI GDGCHC 3 cut(s) 100, 174, 284
MlsI TGGCCA 1 cut(s) 74
MluCI AATT 3 cut(s) 63, 359, 439
MluNI TGGCCA 1 cut(s) 74
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 8 cut(s) 43, 46, 145, 263, 400, 418, 453, 562
Mox20I TGGCCA 1 cut(s) 74
Mph1103I ATGCAT 1 cut(s) 346
MscI TGGCCA 1 cut(s) 74
MslI CAYNNNNRTG 1 cut(s) 317
Msp20I TGGCCA 1 cut(s) 74
MspR9I CCNGG 2 cut(s) 244, 278
MvaI CCWGG 2 cut(s) 244, 278
MvnI CGCG 2 cut(s) 139, 141
MwoI GCNNNNNNNGC 3 cut(s) 104, 350, 537
NcoI CCATGG 1 cut(s) 195
NdeI CATATG 1 cut(s) 187
NdeII GATC 3 cut(s) 57, 236, 466
NlaIII CATG 2 cut(s) 73, 199
NlaIV GGNNCC 5 cut(s) 112, 133, 209, 282, 283
NmuCI GTSAC 1 cut(s) 562
NsiI ATGCAT 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 530
PfeI GAWTC 2 cut(s) 247, 515
PflMI CCANNNNNTGG 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 242
Ppu21I YACGTR 1 cut(s) 91
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 242, 276
PspGI CCWGG 2 cut(s) 242, 276
PspN4I GGNNCC 5 cut(s) 112, 133, 209, 282, 283
PspOMI GGGCCC 1 cut(s) 280
PspPI GGNCC 2 cut(s) 280, 281
PsuI RGATCY 2 cut(s) 57, 466
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
RseI CAYNNNNRTG 1 cut(s) 317
SacI GAGCTC 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 530
Sau3AI GATC 3 cut(s) 57, 236, 466
Sau96I GGNCC 2 cut(s) 280, 281
ScrFI CCNGG 2 cut(s) 244, 278
SduI GDGCHC 3 cut(s) 100, 174, 284
SfaNI GCATC 3 cut(s) 331, 340, 392
SmiMI CAYNNNNRTG 1 cut(s) 317
Sse9I AATT 3 cut(s) 63, 359, 439
SsiI CCGC 3 cut(s) 107, 274, 457
SspMI CTAG 3 cut(s) 35, 234, 452
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 2 cut(s) 242, 276
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 163, 207
TaiI ACGT 2 cut(s) 93, 489
TaqI TCGA 2 cut(s) 334, 507
TasI AATT 3 cut(s) 63, 359, 439
TfiI GAWTC 2 cut(s) 247, 515
TscAI CASTG 2 cut(s) 324, 571
TseFI GTSAC 1 cut(s) 562
Tsp45I GTSAC 1 cut(s) 562
TspDTI ATGAA 4 cut(s) 195, 216, 301, 320
TspRI CASTG 2 cut(s) 324, 571
Van91I CCANNNNNTGG 1 cut(s) 277
XapI RAATTY 2 cut(s) 359, 439
XspI CTAG 3 cut(s) 35, 234, 452
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.