RchiOBHm_Chr7g0242471

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0021465
Physical Location & Seq
Reverse (-)
68543785 .. 68545778
1994 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1797 bp
ATGTCAAGTCTTCTTGCAGCTGAAAACGATGCCAAAGAATTCGAGCGCTACATACCCTTCTCTAACTTTGTGATAAATGGTGATTGGGAGAATGCAAAGGAATGTCTTACAGAACTTCCAGATGCAATAACAGCAAGAAACAAATTTGGAGACATAGCTCTTCATATAGCAGCCAGGAAAGGGCACTTGCATATCGTGAAAGAGTTGGTCTTATTGATGGGACAAGATGATTTAAAATCCAAAAGCGCTGATGGTCTCACAGCTCTTCATCTAGCATTTAGGAACGGGCGCTTTGACACTGTTAAAGAGCTGTTGCCCGTGATGGGAGGAGTAAAAGACTGCTATGGTAACACGGTTCTCCACATGGCATTACTTATGCAGCAACCACGCACTGTAAAGTGGATGGTGAAGTTAATGAAAAGAGAAGACTTGGAATTAAAAAACAGCGATGGTTATACAGCTCTGAAATTAGCAGTCAAGAAGGGGAATGTGTCTACTATTAAAGAGTTGCTGCCGTACGATGATGACTTTGACCGTTATATACCGTTCACTAATGCTGTAATTGAAGGTGATTGGAATAATGCAAAGTATTGTCTTAGAGATCTAGATCCCTACAGGGCAGTAAGAGCAAGAGATCCAAGAGATGGACATGAGAACACGGCTCTTCACTCAGCAATCAAGCATGGGCATGTGGATATTGTGAAAGAGTTGGTGTCGTTGATGACACAAGAAGATTTGGAAGTGAAAAATGCTGATGCTTTCACGGGTAAGTTAAATGCAGACCTCAGTGAACGAGCAGTCGTTGAAATGGCTAAGTACATGGTTGAACAGAATGAGATACTGCTTGCCCGTGTTTTAGAACTAGAGAATGCTTTTGGAGACACACCTCTTCACGAAGCAGTCAGTAAGGGGGATATGTCTATTGTGGAAGAATTGGTACCTTCGATGAGACAAGAAGGTTTTGAAATAAAAAATAGTAATGGTTTTACAGCTCTTCACAAAGCAGTCATGAACGAAAATGTGGATATTGTAAAGATTATAGTCCGGCGGATGAGACGAGAAGGTCTGGAAGTAAAAGGTGCTCTTGGTTACACAGCACTTCACCAAGCAGTCAAGATGGGGAATATGGGGATTGTGAAAGAGTTGGTATTGTTGATGAGACAAGAAGGTCTTGTAGTAAAAAACGACGAGGGTTCCAATGCTCTTCACCTAGCAGTCATGAATGGGAATGTGGACATCGTGAAGATGTTGGTCTCACTGATGAGACGAGAAGATTTGGAAATAAAAGGCGCTCTTGGTTACACAGCACTTCACCAAGCAGTGAATATGGGAAAGACGGGGATTGTGAAAGAGTTGGTATTGGTGATGAGACAAGAGGGTCTTGAAGTAAAAAACGATAAGGGTTCCAATGCTCTTCACCTAGCAGTCATGGAGAAAAAGAATATGGACATTGTGAGAGAGTTGATGTCGTTGATGAGACCACAAGCGATGGAAATAAGAAATGGTGAAGGTTTTACAGTTTTAGGTTGTGCTATGAAGGTAGGCCACAACGATGGAGACATTTGGGAAATGGCTAAATACATGGCTGAAAAGAACAAAAAAGTATTTGGCACTCGCTCAGGTATAGACATTCCAGTTGTATGGGCTGCTGGCTGGGGCAAATGGAAATTAACTCACTATCTCTATTCCGTCACTCCTTGTGAAGCTCTAAACGGGCTTGACGGCGCTAAATTTATTAGTATTGCTATTGAACGTCTGGAAGCGCTTGGTAAGAGTGTTTTAAATATCATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

598

Amino Acids

66.81

Weight (kDa)

6.32

Isoelectric Point (pI)

25.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 35 - 105 4.3e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 54 - 137 6.2e-15 Ankyrin repeats (3 copies)
Ank_2 PF12796 111 - 177 4.7e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 138 - 240 7.6e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 287 - 348 7.9e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 361 - 419 2.5e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 367 - 453 4.5e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 367 - 418 9.4e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 393 - 438 2.8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 425 - 489 1.2e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 437 - 518 3.9e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021465)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0242411 RchiOBHm_Chr7g0242471
rosa_multiflora Rmu_sc0000429.1_g000058 Rmu_sc0000429.1_g000066
rosa_rugosa Rorug04G0006800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 1062, 1167, 1377
Acc65I GGTACC 1 cut(s) 937
AccB1I GGYRCC 1 cut(s) 937
AccB7I CCANNNNNTGG 1 cut(s) 644
AccI GTMKAC 1 cut(s) 494
AciI CCGC 1 cut(s) 1048
AclWI GGATC 2 cut(s) 602, 629
AcsI RAATTY 3 cut(s) 38, 143, 1730
AfaI GTAC 3 cut(s) 518, 818, 939
AfeI AGCGCT 3 cut(s) 47, 247, 1764
AfiI CCNNNNNNNGG 3 cut(s) 180, 323, 644
AgsI TTSAA 6 cut(s) 566, 806, 827, 965, 1385, 1751
AjnI CCWGG 1 cut(s) 173
AluBI AGCT 7 cut(s) 20, 158, 263, 310, 461, 992, 1706
AluI AGCT 7 cut(s) 20, 158, 263, 310, 461, 992, 1706
Alw21I GWGCWC 1 cut(s) 1084
AlwI GGATC 2 cut(s) 602, 629
Aor51HI AGCGCT 3 cut(s) 47, 247, 1764
AoxI GGCC 1 cut(s) 1543
ApeKI GCWGC 5 cut(s) 17, 170, 379, 511, 1646
ApoI RAATTY 3 cut(s) 38, 143, 1730
AseI ATTAAT 1 cut(s) 1791
Asp718I GGTACC 1 cut(s) 937
AspLEI GCGC 6 cut(s) 48, 248, 291, 1292, 1727, 1765
AsuHPI GGTGA 9 cut(s) 92, 418, 581, 1094, 1199, 1304, 1375, 1409, 1517
BaeGI GKGCMC 1 cut(s) 186
BanI GGYRCC 1 cut(s) 937
BarI GAAGNNNNNNTAC 2 cut(s) 921, 953
BbsI GAAGAC 2 cut(s) 2, 432
Bbv12I GWGCWC 1 cut(s) 1084
BbvI GCAGC 5 cut(s) 29, 182, 391, 498, 1633
BccI CCATC 9 cut(s) 211, 245, 316, 397, 443, 638, 1111, 1483, 1547
BceAI ACGGC 3 cut(s) 499, 675, 1738
BciT130I CCWGG 1 cut(s) 175
BfaI CTAG 5 cut(s) 272, 605, 863, 1211, 1421
BfmI CTRYAG 1 cut(s) 613
BfoI RGCGCY 6 cut(s) 49, 249, 292, 1293, 1728, 1766
BglII AGATCT 1 cut(s) 601
BisI GCNGC 5 cut(s) 18, 171, 380, 512, 1647
BlsI GCNGC 5 cut(s) 19, 172, 381, 513, 1648
Bme1390I CCNGG 1 cut(s) 175
BmiI GGNNCC 3 cut(s) 939, 1195, 1405
BmrFI CCNGG 1 cut(s) 175
BmsI GCATC 3 cut(s) 19, 112, 745
BpiI GAAGAC 2 cut(s) 2, 432
Bpu10I CCTNAGC 1 cut(s) 1618
BsaBI GATNNNNATC 1 cut(s) 606
BsaI GGTCTC 3 cut(s) 260, 1258, 1471
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 323, 644
Bse1I ACTGG 1 cut(s) 1634
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 1 cut(s) 175
BseGI GGATG 2 cut(s) 408, 1056
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 3 cut(s) 180, 323, 644
BseMII CTCAG 3 cut(s) 684, 799, 1632
BseNI ACTGG 1 cut(s) 1634
BseRI GAGGAG 1 cut(s) 342
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 5 cut(s) 29, 182, 391, 498, 1633
BseYI CCCAGC 1 cut(s) 1653
BshFI GGCC 1 cut(s) 1545
BshNI GGYRCC 1 cut(s) 937
BsiHKAI GWGCWC 1 cut(s) 1084
BsiSI CCGG 1 cut(s) 1045
BsiWI CGTACG 1 cut(s) 516
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 3 cut(s) 180, 323, 644
BsmBI CGTCTC 2 cut(s) 1048, 1258
BsmFI GGGAC 1 cut(s) 234
BsmI GAATGC 2 cut(s) 97, 874
BsnI GGCC 1 cut(s) 1545
Bso31I GGTCTC 3 cut(s) 260, 1258, 1471
Bsp1286I GDGCHC 2 cut(s) 186, 1084
Bsp143I GATC 3 cut(s) 601, 607, 634
BspACI CCGC 1 cut(s) 1048
BspANI GGCC 1 cut(s) 1545
BspCNI CTCAG 3 cut(s) 683, 798, 1631
BspHI TCATGA 2 cut(s) 1008, 1218
BspLI GGNNCC 3 cut(s) 939, 1195, 1405
BspPI GGATC 2 cut(s) 602, 629
BspQI GCTCTTC 6 cut(s) 165, 270, 669, 999, 1209, 1419
BspT107I GGYRCC 1 cut(s) 937
BspTNI GGTCTC 3 cut(s) 260, 1258, 1471
BsrI ACTGG 1 cut(s) 1634
BssMI GATC 3 cut(s) 601, 607, 634
Bst2UI CCWGG 1 cut(s) 175
Bst4CI ACNGT 6 cut(s) 301, 355, 394, 536, 546, 1519
Bst6I CTCTTC 7 cut(s) 165, 270, 669, 894, 999, 1209, 1419
BstC8I GCNNGC 2 cut(s) 846, 1651
BstDEI CTNAG 5 cut(s) 596, 670, 785, 813, 1618
BstF5I GGATG 2 cut(s) 408, 1056
BstH2I RGCGCY 6 cut(s) 49, 249, 292, 1293, 1728, 1766
BstHHI GCGC 6 cut(s) 48, 248, 291, 1292, 1727, 1765
BstKTI GATC 3 cut(s) 604, 610, 637
BstMBI GATC 3 cut(s) 601, 607, 634
BstMWI GCNNNNNNNGC 2 cut(s) 131, 626
BstNI CCWGG 1 cut(s) 175
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 1 cut(s) 173
BstSFI CTRYAG 1 cut(s) 613
BstSLI GKGCMC 1 cut(s) 186
BstV1I GCAGC 5 cut(s) 29, 182, 391, 498, 1633
BstV2I GAAGAC 2 cut(s) 2, 432
BstX2I RGATCY 3 cut(s) 601, 607, 634
BstXI CCANNNNNNTGG 2 cut(s) 1553, 1641
BstYI RGATCY 3 cut(s) 601, 607, 634
BsuRI GGCC 1 cut(s) 1545
BtgZI GCGATG 2 cut(s) 462, 1502
BtsCI GGATG 2 cut(s) 408, 1056
BtsI GCAGTG 1 cut(s) 1326
BtsIMutI CAGTG 5 cut(s) 297, 390, 793, 1256, 1326
Cac8I GCNNGC 2 cut(s) 846, 1651
CciI TCATGA 2 cut(s) 1008, 1218
CfoI GCGC 6 cut(s) 48, 248, 291, 1292, 1727, 1765
Csp6I GTAC 3 cut(s) 517, 817, 938
CviAII CATG 9 cut(s) 364, 650, 683, 689, 820, 1009, 1219, 1429, 1582
CviQI GTAC 3 cut(s) 517, 817, 938
DdeI CTNAG 5 cut(s) 596, 670, 785, 813, 1618
DpnI GATC 3 cut(s) 603, 609, 636
DpnII GATC 3 cut(s) 601, 607, 634
DraI TTTAAA 2 cut(s) 234, 1782
DrdI GACNNNNNNGTC 3 cut(s) 1062, 1167, 1377
DseDI GACNNNNNNGTC 3 cut(s) 1062, 1167, 1377
Eam1104I CTCTTC 7 cut(s) 165, 270, 669, 894, 999, 1209, 1419
EarI CTCTTC 7 cut(s) 165, 270, 669, 894, 999, 1209, 1419
EciI GGCGGA 1 cut(s) 1063
Eco31I GGTCTC 3 cut(s) 260, 1258, 1471
Eco47III AGCGCT 3 cut(s) 47, 247, 1764
EcoRI GAATTC 1 cut(s) 38
EcoRII CCWGG 1 cut(s) 173
Esp3I CGTCTC 2 cut(s) 1048, 1258
FaeI CATG 9 cut(s) 367, 653, 686, 692, 823, 1012, 1222, 1432, 1585
FalI AAGNNNNNCTT 8 cut(s) 1068, 1100, 1155, 1187, 1278, 1310, 1365, 1397
FaqI GGGAC 1 cut(s) 234
FatI CATG 9 cut(s) 363, 649, 682, 688, 819, 1008, 1218, 1428, 1581
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 5 cut(s) 18, 171, 380, 512, 1647
FokI GGATG 2 cut(s) 415, 1063
Fsp4HI GCNGC 5 cut(s) 18, 171, 380, 512, 1647
FspBI CTAG 5 cut(s) 272, 605, 863, 1211, 1421
GlaI GCGC 6 cut(s) 47, 247, 290, 1291, 1726, 1764
GluI GCNGC 5 cut(s) 18, 171, 380, 512, 1647
GsaI CCCAGC 1 cut(s) 1657
HaeII RGCGCY 6 cut(s) 49, 249, 292, 1293, 1728, 1766
HaeIII GGCC 1 cut(s) 1545
HapII CCGG 1 cut(s) 1045
HhaI GCGC 6 cut(s) 48, 248, 291, 1292, 1727, 1765
Hin1II CATG 9 cut(s) 367, 653, 686, 692, 823, 1012, 1222, 1432, 1585
Hin6I GCGC 6 cut(s) 46, 246, 289, 1290, 1725, 1763
HinP1I GCGC 6 cut(s) 46, 246, 289, 1290, 1725, 1763
HpaII CCGG 1 cut(s) 1045
HphI GGTGA 9 cut(s) 92, 418, 581, 1094, 1199, 1304, 1375, 1409, 1517
Hpy166II GTNNAC 4 cut(s) 495, 549, 791, 1234
Hpy188I TCNGA 1 cut(s) 465
Hpy8I GTNNAC 4 cut(s) 495, 549, 791, 1234
Hpy99I CGWCG 1 cut(s) 1190
HpyAV CCTTC 9 cut(s) 67, 475, 560, 950, 951, 1055, 1160, 1502, 1531
HpyCH4III ACNGT 6 cut(s) 301, 355, 394, 536, 546, 1519
HpyCH4IV ACGT 1 cut(s) 1753
HpyCH4V TGCA 7 cut(s) 17, 95, 125, 190, 379, 584, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 626
HpyF3I CTNAG 5 cut(s) 596, 670, 785, 813, 1618
HpySE526I ACGT 1 cut(s) 1753
Hsp92II CATG 9 cut(s) 367, 653, 686, 692, 823, 1012, 1222, 1432, 1585
HspAI GCGC 6 cut(s) 46, 246, 289, 1290, 1725, 1763
KpnI GGTACC 1 cut(s) 941
Kzo9I GATC 3 cut(s) 601, 607, 634
LguI GCTCTTC 6 cut(s) 165, 270, 669, 999, 1209, 1419
Lsp1109I GCAGC 5 cut(s) 29, 182, 391, 498, 1633
LweI GCATC 3 cut(s) 19, 112, 745
MaeI CTAG 5 cut(s) 272, 605, 863, 1211, 1421
MaeII ACGT 1 cut(s) 1753
MaeIII GTNAC 4 cut(s) 347, 1088, 1298, 1690
MalI GATC 3 cut(s) 603, 609, 636
MboI GATC 3 cut(s) 601, 607, 634
MflI RGATCY 3 cut(s) 601, 607, 634
MhlI GDGCHC 2 cut(s) 186, 1084
MluCI AATT 9 cut(s) 38, 143, 434, 467, 561, 932, 1667, 1730, 1792
MnlI CCTC 5 cut(s) 320, 794, 897, 1183, 1369
MslI CAYNNNNRTG 2 cut(s) 687, 1551
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 1 cut(s) 1045
MspR9I CCNGG 1 cut(s) 175
Mva1269I GAATGC 2 cut(s) 97, 874
MvaI CCWGG 1 cut(s) 175
MwoI GCNNNNNNNGC 2 cut(s) 131, 626
NdeII GATC 3 cut(s) 601, 607, 634
NlaIII CATG 9 cut(s) 367, 653, 686, 692, 823, 1012, 1222, 1432, 1585
NlaIV GGNNCC 3 cut(s) 939, 1195, 1405
NmuCI GTSAC 1 cut(s) 1690
NspI RCATGY 1 cut(s) 692
PacI TTAATTAA 1 cut(s) 1795
PagI TCATGA 2 cut(s) 1008, 1218
PciSI GCTCTTC 6 cut(s) 165, 270, 669, 999, 1209, 1419
PctI GAATGC 2 cut(s) 97, 874
Pfl23II CGTACG 1 cut(s) 516
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 5 cut(s) 19, 172, 381, 513, 1648
PshBI ATTAAT 1 cut(s) 1791
Psp6I CCWGG 1 cut(s) 173
PspFI CCCAGC 1 cut(s) 1653
PspGI CCWGG 1 cut(s) 173
PspLI CGTACG 1 cut(s) 516
PspN4I GGNNCC 3 cut(s) 939, 1195, 1405
PsuI RGATCY 3 cut(s) 601, 607, 634
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 3 cut(s) 518, 818, 939
RsaNI GTAC 3 cut(s) 517, 817, 938
RseI CAYNNNNRTG 2 cut(s) 687, 1551
SapI GCTCTTC 6 cut(s) 165, 270, 669, 999, 1209, 1419
SatI GCNGC 5 cut(s) 18, 171, 380, 512, 1647
Sau3AI GATC 3 cut(s) 601, 607, 634
ScrFI CCNGG 1 cut(s) 175
SduI GDGCHC 2 cut(s) 186, 1084
SfaNI GCATC 3 cut(s) 19, 112, 745
SfcI CTRYAG 1 cut(s) 613
SmiMI CAYNNNNRTG 2 cut(s) 687, 1551
Sse9I AATT 9 cut(s) 38, 143, 434, 467, 561, 932, 1667, 1730, 1792
SsiI CCGC 1 cut(s) 1048
SspMI CTAG 5 cut(s) 272, 605, 863, 1211, 1421
StyD4I CCNGG 1 cut(s) 173
TaaI ACNGT 6 cut(s) 301, 355, 394, 536, 546, 1519
TaiI ACGT 1 cut(s) 1756
TaqI TCGA 2 cut(s) 42, 944
TasI AATT 9 cut(s) 38, 143, 434, 467, 561, 932, 1667, 1730, 1792
TatI WGTACW 1 cut(s) 816
TscAI CASTG 5 cut(s) 304, 397, 793, 1263, 1326
TseFI GTSAC 1 cut(s) 1690
TseI GCWGC 5 cut(s) 17, 170, 379, 511, 1646
Tsp45I GTSAC 1 cut(s) 1690
TspDTI ATGAA 6 cut(s) 152, 257, 431, 1025, 1235, 1550
TspGWI ACGGA 1 cut(s) 1678
TspRI CASTG 5 cut(s) 304, 397, 793, 1263, 1326
Van91I CCANNNNNTGG 1 cut(s) 644
VspI ATTAAT 1 cut(s) 1791
XapI RAATTY 3 cut(s) 38, 143, 1730
XbaI TCTAGA 1 cut(s) 604
XceI RCATGY 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 5 cut(s) 272, 605, 863, 1211, 1421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.