Rmu_sc0000429.1_g000058

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000429.1
OG0021465
Physical Location & Seq
Reverse (-)
253669 .. 255666
1998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000429.1_g000058.1.cds

Sequence Viewer

Length: 1800 bp
atgtcaagtcttcttgcagctgaaaacgatgccaaggaaatcgagcgctacatacccttctctaactttgtgataaatggtgattgggagaatgcaaaggagtgtcttacaaaacttccagatgcaataacagcaagataccaatttggagccacagcccttcatatagcagccgggcaagggcacctgcatatcgtgaaagagttggtcttgctgatgggacaagatgatttacaaactaaaaacgcttatggttacacagctcttcatgtagcattcttcaacgggcgctttgacattgttaaagagctgttgcccctgatgggaggagtaaaagactgccatggtaacacggttcttcacatggcattaattaggcagcaaccacacactgtagagtggatggtgaagttaatgaaaagagaagacttggaattaaaaaacaacgatggttctacagctctgaacatagcagtcaagaaggggaatgtgtctactattaaagagttgctgccgcatgatgatgactttgaccgttatataccgtttactaatgctgtaattgaccgtgattggaataatgcaaactattgtcttagagatctagatccctacagggcagtaagagcaagagatccaagagatggaaatgagaacacggctcttcacttagcaatcaagcatgggcatgtggatattgtgaaagagttggtgtcgttgatgacacaagaagatttggaagtgaaaaatgctgatccttctacgggtaatttaaatgcagacctcagtgaacgagcagtcgtcgaaatggctaagtacatggttgaacagaatgagatactgcttgcccgtgttttagaactagagaatgcttttggagatacacctattcacgaagcagtcagtaagggggatatgtctattgtggaagaattggtaccttcgatgagacaagaaggttttgaaataaaaaataataatggttttacagctcttcaccaagcagtcatgaacgaaaatgtggatattgtgaagattatagtccggcggatgaggcgagaaggtctggaagtaaaaggtgctcttggttacacagcacttcaccaagcagtgaagatggggaatatggggattgtgaaagagattgtattgttgatgagacaagaaggtttcgtagtaaaaaacgacgagggtttcaatgctcttcacctagcagtcatgaagggggatgtggatatcgtgaagatgttggtctcactgatgagacgagatgatttggaaataaaaggcgctcttggttacacagcacttcaccaagcagtgaagatggggaatatggggattgtgaaagagttgctattggtgatgagacaagagggtcttgaagtaaaaaatgacgcgggttccaatgctcttcaccttgcagtcatggagaataagaatatgggcattgcgagagagttgatgtcgttgatgagaccgcaagcgatggaaataaaaaatggtcaaggttttacagctttaggttgtgctatggagcttggcgccaaagatggagacttttgggaaatagctaagtacatggccggaaagaacaaaaatgtatttggcattcgctcgggtagattcattccagttgtatgggctgctgcctggtgcaaatggaaattaactcactgtctctattccgtcactccacgtgaagttctaaacgggtttaacggcgctcagttaattacttttgctattgaacgtctgcaagcgcttggtaagagtgttttaaatatcaattattattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

599

Amino Acids

67.0

Weight (kDa)

6.09

Isoelectric Point (pI)

27.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021465)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0242411 RchiOBHm_Chr7g0242471
rosa_multiflora Rmu_sc0000429.1_g000058 Rmu_sc0000429.1_g000066
rosa_rugosa Rorug04G0006800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 195
AasI GACNNNNNNGTC 1 cut(s) 1377
Acc36I ACCTGC 1 cut(s) 195
Acc65I GGTACC 1 cut(s) 937
AccB1I GGYRCC 3 cut(s) 183, 937, 1544
AccB7I CCANNNNNTGG 1 cut(s) 644
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 1400
AciI CCGC 4 cut(s) 515, 1048, 1400, 1481
AclWI GGATC 3 cut(s) 602, 629, 749
AcoI YGGCCR 1 cut(s) 1584
AcvI CACGTG 1 cut(s) 1700
AcyI GRCGYC 1 cut(s) 1545
AfaI GTAC 3 cut(s) 818, 939, 1580
AfeI AGCGCT 2 cut(s) 47, 1764
AfiI CCNNNNNNNGG 4 cut(s) 180, 323, 644, 764
AgsI TTSAA 6 cut(s) 283, 827, 965, 1198, 1385, 1751
AjnI CCWGG 1 cut(s) 1652
AluBI AGCT 8 cut(s) 20, 263, 310, 461, 992, 1520, 1540, 1574
AluI AGCT 8 cut(s) 20, 263, 310, 461, 992, 1520, 1540, 1574
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 8 cut(s) 943, 1153, 1258, 1258, 1363, 1471, 1551, 1685
AlwI GGATC 3 cut(s) 602, 629, 749
Ama87I CYCGRG 1 cut(s) 1618
Aor51HI AGCGCT 2 cut(s) 47, 1764
AoxI GGCC 1 cut(s) 1584
ApeKI GCWGC 6 cut(s) 17, 170, 379, 511, 1646, 1649
AseI ATTAAT 1 cut(s) 371
Asp718I GGTACC 1 cut(s) 937
AspLEI GCGC 6 cut(s) 48, 291, 1292, 1547, 1727, 1765
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 8 cut(s) 92, 418, 989, 1094, 1199, 1304, 1375, 1409
AvaI CYCGRG 1 cut(s) 1618
BaeGI GKGCMC 1 cut(s) 186
BanI GGYRCC 3 cut(s) 183, 937, 1544
BarI GAAGNNNNNNTAC 2 cut(s) 921, 953
BbrPI CACGTG 1 cut(s) 1700
BbsI GAAGAC 2 cut(s) 2, 432
Bbv12I GWGCWC 1 cut(s) 1084
BbvI GCAGC 6 cut(s) 29, 182, 391, 498, 1633, 1636
BccI CCATC 9 cut(s) 211, 316, 397, 443, 638, 1111, 1321, 1483, 1547
BceAI ACGGC 2 cut(s) 675, 1738
BciT130I CCWGG 1 cut(s) 1654
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 8 cut(s) 943, 1153, 1258, 1258, 1363, 1471, 1551, 1685
BfaI CTAG 3 cut(s) 605, 863, 1211
BfmI CTRYAG 3 cut(s) 393, 456, 613
BfoI RGCGCY 6 cut(s) 49, 292, 1293, 1548, 1728, 1766
BfuAI ACCTGC 1 cut(s) 195
BglII AGATCT 1 cut(s) 601
BisI GCNGC 7 cut(s) 18, 171, 380, 512, 515, 1647, 1650
BlsI GCNGC 7 cut(s) 19, 172, 381, 513, 516, 1648, 1651
Bme1390I CCNGG 2 cut(s) 175, 1654
BmeT110I CYCGRG 1 cut(s) 1618
BmiI GGNNCC 5 cut(s) 151, 185, 939, 1405, 1546
BmrFI CCNGG 2 cut(s) 175, 1654
BmsI GCATC 2 cut(s) 19, 112
BpiI GAAGAC 2 cut(s) 2, 432
BpuMI CCSGG 1 cut(s) 175
BsaAI YACGTR 1 cut(s) 1700
BsaBI GATNNNNATC 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 1545
BsaI GGTCTC 2 cut(s) 1258, 1471
BsaJI CCNNGG 2 cut(s) 33, 343
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 323, 644, 764
Bse1I ACTGG 1 cut(s) 1634
Bse3DI GCAATG 1 cut(s) 1449
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 1 cut(s) 1654
BseDI CCNNGG 2 cut(s) 33, 343
BseGI GGATG 3 cut(s) 408, 1056, 1234
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 4 cut(s) 180, 323, 644, 764
BseMI GCAATG 1 cut(s) 1449
BseMII CTCAG 2 cut(s) 799, 1742
BseNI ACTGG 1 cut(s) 1634
BseRI GAGGAG 1 cut(s) 342
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 6 cut(s) 29, 182, 391, 498, 1633, 1636
Bsh1236I CGCG 1 cut(s) 1400
BshFI GGCC 1 cut(s) 1586
BshNI GGYRCC 3 cut(s) 183, 937, 1544
BsiHKAI GWGCWC 1 cut(s) 1084
BsiHKCI CYCGRG 1 cut(s) 1618
BsiSI CCGG 3 cut(s) 174, 1045, 1587
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 4 cut(s) 180, 323, 644, 764
BsmAI GTCTC 8 cut(s) 943, 1153, 1258, 1258, 1363, 1471, 1551, 1685
BsmBI CGTCTC 1 cut(s) 1258
BsmFI GGGAC 1 cut(s) 234
BsmI GAATGC 4 cut(s) 97, 275, 874, 1611
BsnI GGCC 1 cut(s) 1586
Bso31I GGTCTC 2 cut(s) 1258, 1471
BsoBI CYCGRG 1 cut(s) 1618
Bsp1286I GDGCHC 2 cut(s) 186, 1084
Bsp143I GATC 4 cut(s) 601, 607, 634, 754
Bsp19I CCATGG 1 cut(s) 343
BspACI CCGC 4 cut(s) 515, 1048, 1400, 1481
BspANI GGCC 1 cut(s) 1586
BspCNI CTCAG 2 cut(s) 798, 1741
BspFNI CGCG 1 cut(s) 1400
BspHI TCATGA 2 cut(s) 1008, 1218
BspLI GGNNCC 5 cut(s) 151, 185, 939, 1405, 1546
BspMI ACCTGC 1 cut(s) 195
BspPI GGATC 3 cut(s) 602, 629, 749
BspQI GCTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
BspT107I GGYRCC 3 cut(s) 183, 937, 1544
BspTNI GGTCTC 2 cut(s) 1258, 1471
BsrDI GCAATG 1 cut(s) 1449
BsrI ACTGG 1 cut(s) 1634
BssECI CCNNGG 2 cut(s) 33, 343
BssMI GATC 4 cut(s) 601, 607, 634, 754
BssNI GRCGYC 1 cut(s) 1545
BssT1I CCWWGG 2 cut(s) 33, 343
Bst2UI CCWGG 1 cut(s) 1654
Bst4CI ACNGT 6 cut(s) 355, 394, 536, 546, 569, 1679
Bst6I CTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
BstACI GRCGYC 1 cut(s) 1545
BstBAI YACGTR 1 cut(s) 1700
BstC8I GCNNGC 3 cut(s) 846, 1485, 1761
BstDEI CTNAG 6 cut(s) 596, 670, 785, 813, 1575, 1728
BstDSI CCRYGG 1 cut(s) 343
BstF5I GGATG 3 cut(s) 408, 1056, 1234
BstFNI CGCG 1 cut(s) 1400
BstH2I RGCGCY 6 cut(s) 49, 292, 1293, 1548, 1728, 1766
BstHHI GCGC 6 cut(s) 48, 291, 1292, 1547, 1727, 1765
BstKTI GATC 4 cut(s) 604, 610, 637, 757
BstMAI GTCTC 8 cut(s) 943, 1153, 1258, 1258, 1363, 1471, 1551, 1685
BstMBI GATC 4 cut(s) 601, 607, 634, 754
BstMWI GCNNNNNNNGC 3 cut(s) 131, 626, 1054
BstNI CCWGG 1 cut(s) 1654
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 2 cut(s) 173, 1652
BstSFI CTRYAG 3 cut(s) 393, 456, 613
BstSLI GKGCMC 1 cut(s) 186
BstUI CGCG 1 cut(s) 1400
BstV1I GCAGC 6 cut(s) 29, 182, 391, 498, 1633, 1636
BstV2I GAAGAC 2 cut(s) 2, 432
BstX2I RGATCY 3 cut(s) 601, 607, 634
BstXI CCANNNNNNTGG 1 cut(s) 1641
BstYI RGATCY 3 cut(s) 601, 607, 634
BsuRI GGCC 1 cut(s) 1586
BtgI CCRYGG 1 cut(s) 343
BtgZI GCGATG 1 cut(s) 1502
BtsCI GGATG 3 cut(s) 408, 1056, 1234
BtsI GCAGTG 2 cut(s) 1116, 1326
BtsIMutI CAGTG 6 cut(s) 390, 793, 1116, 1256, 1326, 1675
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 3 cut(s) 846, 1485, 1761
CciI TCATGA 2 cut(s) 1008, 1218
CfoI GCGC 6 cut(s) 48, 291, 1292, 1547, 1727, 1765
CseI GACGC 1 cut(s) 1406
Csp6I GTAC 3 cut(s) 817, 938, 1579
CviQI GTAC 3 cut(s) 817, 938, 1579
DdeI CTNAG 6 cut(s) 596, 670, 785, 813, 1575, 1728
DinI GGCGCC 1 cut(s) 1546
DpnI GATC 4 cut(s) 603, 609, 636, 756
DpnII GATC 4 cut(s) 601, 607, 634, 754
DraI TTTAAA 2 cut(s) 774, 1782
DrdI GACNNNNNNGTC 1 cut(s) 1377
DseDI GACNNNNNNGTC 1 cut(s) 1377
EaeI YGGCCR 1 cut(s) 1584
Eam1104I CTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
EarI CTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
EciI GGCGGA 1 cut(s) 1063
Eco130I CCWWGG 2 cut(s) 33, 343
Eco31I GGTCTC 2 cut(s) 1258, 1471
Eco32I GATATC 1 cut(s) 1237
Eco47III AGCGCT 2 cut(s) 47, 1764
Eco72I CACGTG 1 cut(s) 1700
Eco88I CYCGRG 1 cut(s) 1618
EcoRII CCWGG 1 cut(s) 1652
EcoRV GATATC 1 cut(s) 1237
EcoT14I CCWWGG 2 cut(s) 33, 343
EgeI GGCGCC 1 cut(s) 1546
EheI GGCGCC 1 cut(s) 1546
ErhI CCWWGG 2 cut(s) 33, 343
Esp3I CGTCTC 1 cut(s) 1258
FalI AAGNNNNNCTT 6 cut(s) 1068, 1100, 1278, 1310, 1365, 1397
FaqI GGGAC 1 cut(s) 234
FauI CCCGC 1 cut(s) 1393
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 7 cut(s) 18, 171, 380, 512, 515, 1647, 1650
FokI GGATG 3 cut(s) 415, 1063, 1241
Fsp4HI GCNGC 7 cut(s) 18, 171, 380, 512, 515, 1647, 1650
FspBI CTAG 3 cut(s) 605, 863, 1211
GlaI GCGC 6 cut(s) 47, 290, 1291, 1546, 1726, 1764
GluI GCNGC 7 cut(s) 18, 171, 380, 512, 515, 1647, 1650
HaeII RGCGCY 6 cut(s) 49, 292, 1293, 1548, 1728, 1766
HaeIII GGCC 1 cut(s) 1586
HapII CCGG 3 cut(s) 174, 1045, 1587
HgaI GACGC 1 cut(s) 1406
HhaI GCGC 6 cut(s) 48, 291, 1292, 1547, 1727, 1765
Hin1I GRCGYC 1 cut(s) 1545
Hin6I GCGC 6 cut(s) 46, 289, 1290, 1545, 1725, 1763
HinP1I GCGC 6 cut(s) 46, 289, 1290, 1545, 1725, 1763
HinfI GANTC 1 cut(s) 1626
HpaII CCGG 3 cut(s) 174, 1045, 1587
HphI GGTGA 8 cut(s) 92, 418, 989, 1094, 1199, 1304, 1375, 1409
Hpy166II GTNNAC 3 cut(s) 495, 549, 791
Hpy188I TCNGA 1 cut(s) 465
Hpy8I GTNNAC 3 cut(s) 495, 549, 791
Hpy99I CGWCG 2 cut(s) 806, 1190
HpyAV CCTTC 9 cut(s) 67, 170, 475, 768, 950, 951, 1055, 1160, 1216
HpyCH4III ACNGT 6 cut(s) 355, 394, 536, 546, 569, 1679
HpyCH4IV ACGT 2 cut(s) 1699, 1753
HpyCH4V TGCA 9 cut(s) 17, 95, 125, 190, 584, 779, 1424, 1659, 1759
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 626, 1054
HpyF3I CTNAG 6 cut(s) 596, 670, 785, 813, 1575, 1728
HpySE526I ACGT 2 cut(s) 1699, 1753
Hsp92I GRCGYC 1 cut(s) 1545
HspAI GCGC 6 cut(s) 46, 289, 1290, 1545, 1725, 1763
KasI GGCGCC 1 cut(s) 1544
KpnI GGTACC 1 cut(s) 941
Kzo9I GATC 4 cut(s) 601, 607, 634, 754
LguI GCTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
LmnI GCTCC 2 cut(s) 149, 1537
Lsp1109I GCAGC 6 cut(s) 29, 182, 391, 498, 1633, 1636
LweI GCATC 2 cut(s) 19, 112
MaeI CTAG 3 cut(s) 605, 863, 1211
MaeII ACGT 2 cut(s) 1699, 1753
MaeIII GTNAC 5 cut(s) 254, 347, 1088, 1298, 1690
MalI GATC 4 cut(s) 603, 609, 636, 756
MboI GATC 4 cut(s) 601, 607, 634, 754
MflI RGATCY 3 cut(s) 601, 607, 634
MhlI GDGCHC 2 cut(s) 186, 1084
MluCI AATT 9 cut(s) 143, 372, 434, 561, 769, 932, 1667, 1734, 1789
Mly113I GGCGCC 1 cut(s) 1545
MnlI CCTC 5 cut(s) 320, 794, 1047, 1183, 1369
MslI CAYNNNNRTG 2 cut(s) 522, 687
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 3 cut(s) 174, 1045, 1587
MspR9I CCNGG 2 cut(s) 175, 1654
Mva1269I GAATGC 4 cut(s) 97, 275, 874, 1611
MvaI CCWGG 1 cut(s) 1654
MvnI CGCG 1 cut(s) 1400
MwoI GCNNNNNNNGC 3 cut(s) 131, 626, 1054
NarI GGCGCC 1 cut(s) 1545
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 343
NdeII GATC 4 cut(s) 601, 607, 634, 754
NlaIV GGNNCC 5 cut(s) 151, 185, 939, 1405, 1546
NmuCI GTSAC 1 cut(s) 1690
NspI RCATGY 1 cut(s) 692
PagI TCATGA 2 cut(s) 1008, 1218
PaqCI CACCTGC 1 cut(s) 195
PciSI GCTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
PctI GAATGC 4 cut(s) 97, 275, 874, 1611
PfeI GAWTC 1 cut(s) 1626
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 7 cut(s) 19, 172, 381, 513, 516, 1648, 1651
PluTI GGCGCC 1 cut(s) 1548
PmaCI CACGTG 1 cut(s) 1700
PmlI CACGTG 1 cut(s) 1700
Ppu21I YACGTR 1 cut(s) 1700
PshBI ATTAAT 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 1652
PspCI CACGTG 1 cut(s) 1700
PspGI CCWGG 1 cut(s) 1652
PspN4I GGNNCC 5 cut(s) 151, 185, 939, 1405, 1546
PsuI RGATCY 3 cut(s) 601, 607, 634
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 3 cut(s) 818, 939, 1580
RsaNI GTAC 3 cut(s) 817, 938, 1579
RseI CAYNNNNRTG 2 cut(s) 522, 687
SapI GCTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
SatI GCNGC 7 cut(s) 18, 171, 380, 512, 515, 1647, 1650
Sau3AI GATC 4 cut(s) 601, 607, 634, 754
ScrFI CCNGG 2 cut(s) 175, 1654
SduI GDGCHC 2 cut(s) 186, 1084
SfaNI GCATC 2 cut(s) 19, 112
SfcI CTRYAG 3 cut(s) 393, 456, 613
SfoI GGCGCC 1 cut(s) 1546
SmiI ATTTAAAT 1 cut(s) 774
SmiMI CAYNNNNRTG 2 cut(s) 522, 687
Sse9I AATT 9 cut(s) 143, 372, 434, 561, 769, 932, 1667, 1734, 1789
SsiI CCGC 4 cut(s) 515, 1048, 1400, 1481
SspDI GGCGCC 1 cut(s) 1544
SspMI CTAG 3 cut(s) 605, 863, 1211
StyD4I CCNGG 2 cut(s) 173, 1652
StyI CCWWGG 2 cut(s) 33, 343
SwaI ATTTAAAT 1 cut(s) 774
TaaI ACNGT 6 cut(s) 355, 394, 536, 546, 569, 1679
TaiI ACGT 2 cut(s) 1702, 1756
TaqI TCGA 3 cut(s) 42, 804, 944
TasI AATT 9 cut(s) 143, 372, 434, 561, 769, 932, 1667, 1734, 1789
TatI WGTACW 2 cut(s) 816, 1578
TauI GCSGC 1 cut(s) 517
TfiI GAWTC 1 cut(s) 1626
TscAI CASTG 6 cut(s) 397, 793, 1116, 1263, 1326, 1682
TseFI GTSAC 1 cut(s) 1690
TseI GCWGC 6 cut(s) 17, 170, 379, 511, 1646, 1649
Tsp45I GTSAC 1 cut(s) 1690
TspDTI ATGAA 6 cut(s) 152, 257, 431, 1025, 1235, 1618
TspGWI ACGGA 1 cut(s) 1678
TspRI CASTG 6 cut(s) 397, 793, 1116, 1263, 1326, 1682
Van91I CCANNNNNTGG 1 cut(s) 644
VspI ATTAAT 1 cut(s) 371
XbaI TCTAGA 1 cut(s) 604
XceI RCATGY 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 3 cut(s) 605, 863, 1211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.