Rmu_sc0000429.1_g000066

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000429.1
OG0021465
Physical Location & Seq
Reverse (-)
295160 .. 297083
1924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000429.1_g000066.1.cds

Sequence Viewer

Length: 1608 bp
atggacaaaaatcctaaagatatccctggcattttgatattaatgtcaagtcttcttgcagctgaaaacgaattcgagcgctacatacccttctctagctttgtgaatgatggtgattgggagaatgcaaaggagtgtctcacagaacttccagatgcaataacagcaagaaacgaatttggagacatagctcttcatatagcagccaggcaagggcaccaacatattgtgaaagagttggtcttgctgatgggacaagatgatttacaaaataaaaacgctgatggtctcacagctcttcatgaagcattcatgaaagggcgctttgacactgttaaagagctgttgcccgtgatgggaggagtaaaagacgggcgccatggtaacacggttcttcacatggcattaattatgcaccaaccacgcactgtaaagtggatcgtaaagtggatggtgaagttaatgaaaagagaagacatggacttacaaaacaactatggtcatacagccttgaaattagcagtcaagaagcggaatgtggctactattaaagagttgctgccgtacgtaattgaaggtgattggaataatgcaaaggattatcttagagatctaaatccctacagggcagtaagagcaagagatccaagagatggacatgagaacacggctcttcacgtagcaatcaagcatgggcatgtggatattgtgaaagaattggtgtcgttgatgacacaagaagatttggaagtgaaaaatgctgatcctttcacaggtaatttaaatgcagacctcagtgaacgagcagtcgtcgaaatggctaagtacatggttgaacagaatgagatactgcttgcccgtgttttagaactagagaatgcttttggagacacacctctccacgaagcagtcagtaagggggatatgtctattgtggaagaattggtaccttcgatgagacaagaaggtttcgaaataaaaaataataaaggtgtaacagctcttcaccaagcagtcatgaacgaaaatgtggatatcgtgaagattatagtcccgcggatgagaccagaagatttggaaataaaaaacgacgcgggtcgcaatgttcttcacatagcagtcgtggagaataaggatatggtcattgtgagagagttgatgtcgttgatgagaccacaagctatggaagcaaaagatggtgtcggttctacagctttagattctgctattaacgaaggcgccaacgatggagacattttggaaatggctaaatacatggctggaaagaacaaaaatgtatttggcattcgcaatcgctcgggtagatacattccagttgtatgcgctgctgcctggggcaaatggaaaataactcattatctatattctgtcactccactcgaagctctaagcgggcaagacggcgctcaacttattgcttttgctattgaacgtctgaaagcgcttgatttggcatggaatttactacagcatttcccaagactggcaatagatcctctagcatgtcccctgttctcggtggctactgcagaatatgcattcggagtgcgacccacattctggcaatcgtggatctacgacggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

535

Amino Acids

60.1

Weight (kDa)

5.7

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021465)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0242411 RchiOBHm_Chr7g0242471
rosa_multiflora Rmu_sc0000429.1_g000058 Rmu_sc0000429.1_g000066
rosa_rugosa Rorug04G0006800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 946
AccB1I GGYRCC 4 cut(s) 216, 375, 946, 1238
AccB7I CCANNNNNTGG 2 cut(s) 653, 1581
AccII CGCG 2 cut(s) 1057, 1094
AciI CCGC 5 cut(s) 532, 1055, 1057, 1094, 1413
AclWI GGATC 5 cut(s) 446, 638, 758, 1508, 1601
AcsI RAATTY 3 cut(s) 71, 176, 1480
AcyI GRCGYC 2 cut(s) 376, 1239
AfaI GTAC 3 cut(s) 566, 827, 948
AfeI AGCGCT 2 cut(s) 80, 1464
AfiI CCNNNNNNNGG 7 cut(s) 213, 356, 653, 773, 1504, 1537, 1581
AgsI TTSAA 4 cut(s) 514, 575, 836, 1451
AjnI CCWGG 3 cut(s) 25, 206, 1352
AluBI AGCT 9 cut(s) 62, 99, 191, 296, 343, 1001, 1181, 1214, 1406
AluI AGCT 9 cut(s) 62, 99, 191, 296, 343, 1001, 1181, 1214, 1406
Alw26I GTCTC 8 cut(s) 143, 177, 293, 882, 952, 1057, 1165, 1245
AlwI GGATC 5 cut(s) 446, 638, 758, 1508, 1601
Ama87I CYCGRG 1 cut(s) 1318
Aor51HI AGCGCT 2 cut(s) 80, 1464
ApeKI GCWGC 5 cut(s) 59, 203, 559, 1346, 1349
ApoI RAATTY 3 cut(s) 71, 176, 1480
AseI ATTAAT 2 cut(s) 41, 407
Asp718I GGTACC 1 cut(s) 946
AspLEI GCGC 7 cut(s) 81, 324, 378, 1241, 1346, 1427, 1465
AsuHPI GGTGA 4 cut(s) 125, 466, 590, 998
AsuII TTCGAA 1 cut(s) 972
AvaI CYCGRG 1 cut(s) 1318
BaeGI GKGCMC 1 cut(s) 219
BanI GGYRCC 4 cut(s) 216, 375, 946, 1238
BarI GAAGNNNNNNTAC 4 cut(s) 930, 962, 987, 1019
BbsI GAAGAC 2 cut(s) 44, 480
BbvI GCAGC 5 cut(s) 71, 215, 546, 1333, 1336
BccI CCATC 8 cut(s) 104, 244, 278, 349, 445, 647, 1190, 1241
BceAI ACGGC 3 cut(s) 547, 684, 1438
BciT130I CCWGG 3 cut(s) 27, 208, 1354
BcoDI GTCTC 8 cut(s) 143, 177, 293, 882, 952, 1057, 1165, 1245
BfaI CTAG 3 cut(s) 96, 872, 1520
BfmI CTRYAG 4 cut(s) 622, 1209, 1487, 1548
BfoI RGCGCY 6 cut(s) 82, 325, 379, 1242, 1428, 1466
BglII AGATCT 1 cut(s) 610
BisI GCNGC 5 cut(s) 60, 204, 560, 1347, 1350
BlsI GCNGC 5 cut(s) 61, 205, 561, 1348, 1351
Bme1390I CCNGG 3 cut(s) 27, 208, 1354
BmeT110I CYCGRG 1 cut(s) 1318
BmiI GGNNCC 4 cut(s) 218, 377, 948, 1240
BmrFI CCNGG 3 cut(s) 27, 208, 1354
BmsI GCATC 1 cut(s) 145
BoxI GACNNNNGTC 1 cut(s) 1095
BpiI GAAGAC 2 cut(s) 44, 480
Bpu14I TTCGAA 1 cut(s) 972
BsaAI YACGTR 2 cut(s) 568, 679
BsaBI GATNNNNATC 1 cut(s) 615
BsaHI GRCGYC 2 cut(s) 376, 1239
BsaI GGTCTC 3 cut(s) 293, 1057, 1165
BsaJI CCNNGG 4 cut(s) 25, 379, 1055, 1353
Bsc4I CCNNNNNNNGG 7 cut(s) 213, 356, 653, 773, 1504, 1537, 1581
Bse1I ACTGG 2 cut(s) 1334, 1509
Bse3DI GCAATG 1 cut(s) 1108
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 3 cut(s) 27, 208, 1354
BseDI CCNNGG 4 cut(s) 25, 379, 1055, 1353
BseGI GGATG 2 cut(s) 456, 1065
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 7 cut(s) 213, 356, 653, 773, 1504, 1537, 1581
BseMI GCAATG 1 cut(s) 1108
BseMII CTCAG 1 cut(s) 808
BseNI ACTGG 2 cut(s) 1334, 1509
BseRI GAGGAG 1 cut(s) 375
BseSI GKGCMC 1 cut(s) 219
BseXI GCAGC 5 cut(s) 71, 215, 546, 1333, 1336
Bsh1236I CGCG 2 cut(s) 1057, 1094
BshNI GGYRCC 4 cut(s) 216, 375, 946, 1238
BsiHKCI CYCGRG 1 cut(s) 1318
BsiWI CGTACG 1 cut(s) 564
BslFI GGGAC 3 cut(s) 267, 1037, 1512
BslI CCNNNNNNNGG 7 cut(s) 213, 356, 653, 773, 1504, 1537, 1581
BsmAI GTCTC 8 cut(s) 143, 177, 293, 882, 952, 1057, 1165, 1245
BsmFI GGGAC 3 cut(s) 267, 1037, 1512
BsmI GAATGC 5 cut(s) 130, 308, 883, 1305, 1559
Bso31I GGTCTC 3 cut(s) 293, 1057, 1165
BsoBI CYCGRG 1 cut(s) 1318
Bsp119I TTCGAA 1 cut(s) 972
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 6 cut(s) 438, 610, 643, 763, 1513, 1593
Bsp19I CCATGG 1 cut(s) 379
BspACI CCGC 5 cut(s) 532, 1055, 1057, 1094, 1413
BspCNI CTCAG 1 cut(s) 807
BspFNI CGCG 2 cut(s) 1057, 1094
BspHI TCATGA 3 cut(s) 301, 312, 1017
BspLI GGNNCC 4 cut(s) 218, 377, 948, 1240
BspMAI CTGCAG 1 cut(s) 1552
BspPI GGATC 5 cut(s) 446, 638, 758, 1508, 1601
BspQI GCTCTTC 4 cut(s) 198, 303, 678, 1008
BspT104I TTCGAA 1 cut(s) 972
BspT107I GGYRCC 4 cut(s) 216, 375, 946, 1238
BspTNI GGTCTC 3 cut(s) 293, 1057, 1165
BsrDI GCAATG 1 cut(s) 1108
BsrI ACTGG 2 cut(s) 1334, 1509
BssECI CCNNGG 4 cut(s) 25, 379, 1055, 1353
BssMI GATC 6 cut(s) 438, 610, 643, 763, 1513, 1593
BssNI GRCGYC 2 cut(s) 376, 1239
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 3 cut(s) 27, 208, 1354
Bst4CI ACNGT 4 cut(s) 334, 391, 430, 1604
Bst6I CTCTTC 4 cut(s) 198, 303, 678, 1008
BstACI GRCGYC 2 cut(s) 376, 1239
BstAPI GCANNNNNTGC 1 cut(s) 1556
BstBAI YACGTR 2 cut(s) 568, 679
BstBI TTCGAA 1 cut(s) 972
BstC8I GCNNGC 2 cut(s) 855, 1415
BstDEI CTNAG 4 cut(s) 605, 794, 822, 1409
BstDSI CCRYGG 2 cut(s) 379, 1055
BstENI CCTNNNNNAGG 1 cut(s) 771
BstF5I GGATG 2 cut(s) 456, 1065
BstFNI CGCG 2 cut(s) 1057, 1094
BstH2I RGCGCY 6 cut(s) 82, 325, 379, 1242, 1428, 1466
BstHHI GCGC 7 cut(s) 81, 324, 378, 1241, 1346, 1427, 1465
BstKTI GATC 6 cut(s) 441, 613, 646, 766, 1516, 1596
BstMAI GTCTC 8 cut(s) 143, 177, 293, 882, 952, 1057, 1165, 1245
BstMBI GATC 6 cut(s) 438, 610, 643, 763, 1513, 1593
BstMWI GCNNNNNNNGC 4 cut(s) 164, 635, 1187, 1556
BstNI CCWGG 3 cut(s) 27, 208, 1354
BstNSI RCATGY 2 cut(s) 701, 1527
BstPAI GACNNNNGTC 1 cut(s) 1095
BstSCI CCNGG 3 cut(s) 25, 206, 1352
BstSFI CTRYAG 4 cut(s) 622, 1209, 1487, 1548
BstSLI GKGCMC 1 cut(s) 219
BstSNI TACGTA 1 cut(s) 568
BstUI CGCG 2 cut(s) 1057, 1094
BstV1I GCAGC 5 cut(s) 71, 215, 546, 1333, 1336
BstV2I GAAGAC 2 cut(s) 44, 480
BstX2I RGATCY 4 cut(s) 610, 643, 1513, 1593
BstYI RGATCY 4 cut(s) 610, 643, 1513, 1593
BtgI CCRYGG 2 cut(s) 379, 1055
BtsCI GGATG 2 cut(s) 456, 1065
BtsIMutI CAGTG 3 cut(s) 330, 426, 802
Cac8I GCNNGC 2 cut(s) 855, 1415
CciI TCATGA 3 cut(s) 301, 312, 1017
CfoI GCGC 7 cut(s) 81, 324, 378, 1241, 1346, 1427, 1465
Cfr42I CCGCGG 1 cut(s) 1058
CseI GACGC 1 cut(s) 1100
Csp6I GTAC 3 cut(s) 565, 826, 947
CviQI GTAC 3 cut(s) 565, 826, 947
DdeI CTNAG 4 cut(s) 605, 794, 822, 1409
DinI GGCGCC 2 cut(s) 377, 1240
DpnI GATC 6 cut(s) 440, 612, 645, 765, 1515, 1595
DpnII GATC 6 cut(s) 438, 610, 643, 763, 1513, 1593
DraI TTTAAA 1 cut(s) 783
Eam1104I CTCTTC 4 cut(s) 198, 303, 678, 1008
EarI CTCTTC 4 cut(s) 198, 303, 678, 1008
Eco105I TACGTA 1 cut(s) 568
Eco130I CCWWGG 1 cut(s) 379
Eco31I GGTCTC 3 cut(s) 293, 1057, 1165
Eco32I GATATC 2 cut(s) 22, 1036
Eco47III AGCGCT 2 cut(s) 80, 1464
Eco88I CYCGRG 1 cut(s) 1318
EcoNI CCTNNNNNAGG 1 cut(s) 771
EcoRI GAATTC 1 cut(s) 71
EcoRII CCWGG 3 cut(s) 25, 206, 1352
EcoRV GATATC 2 cut(s) 22, 1036
EcoT14I CCWWGG 1 cut(s) 379
EcoT22I ATGCAT 1 cut(s) 1561
EgeI GGCGCC 2 cut(s) 377, 1240
EheI GGCGCC 2 cut(s) 377, 1240
ErhI CCWWGG 1 cut(s) 379
FaqI GGGAC 3 cut(s) 267, 1037, 1512
FauI CCCGC 3 cut(s) 1062, 1087, 1406
Fnu4HI GCNGC 5 cut(s) 60, 204, 560, 1347, 1350
FokI GGATG 2 cut(s) 463, 1072
Fsp4HI GCNGC 5 cut(s) 60, 204, 560, 1347, 1350
FspBI CTAG 3 cut(s) 96, 872, 1520
GlaI GCGC 7 cut(s) 80, 323, 377, 1240, 1345, 1426, 1464
GluI GCNGC 5 cut(s) 60, 204, 560, 1347, 1350
HaeII RGCGCY 6 cut(s) 82, 325, 379, 1242, 1428, 1466
HgaI GACGC 1 cut(s) 1100
HhaI GCGC 7 cut(s) 81, 324, 378, 1241, 1346, 1427, 1465
Hin1I GRCGYC 2 cut(s) 376, 1239
Hin6I GCGC 7 cut(s) 79, 322, 376, 1239, 1344, 1425, 1463
HinP1I GCGC 7 cut(s) 79, 322, 376, 1239, 1344, 1425, 1463
HinfI GANTC 1 cut(s) 1220
HphI GGTGA 4 cut(s) 125, 466, 590, 998
Hpy166II GTNNAC 1 cut(s) 800
Hpy188I TCNGA 2 cut(s) 1458, 1565
Hpy188III TCNNGA 6 cut(s) 152, 302, 313, 526, 1018, 1039
Hpy8I GTNNAC 1 cut(s) 800
Hpy99I CGWCG 3 cut(s) 815, 1094, 1604
HpyAV CCTTC 5 cut(s) 100, 569, 959, 960, 1229
HpyCH4III ACNGT 4 cut(s) 334, 391, 430, 1604
HpyCH4IV ACGT 3 cut(s) 567, 678, 1453
HpyCH4V TGCA 8 cut(s) 59, 128, 158, 415, 593, 788, 1550, 1559
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 635, 1187, 1556
HpyF3I CTNAG 4 cut(s) 605, 794, 822, 1409
HpySE526I ACGT 3 cut(s) 567, 678, 1453
Hsp92I GRCGYC 2 cut(s) 376, 1239
HspAI GCGC 7 cut(s) 79, 322, 376, 1239, 1344, 1425, 1463
KasI GGCGCC 2 cut(s) 375, 1238
KpnI GGTACC 1 cut(s) 950
KspI CCGCGG 1 cut(s) 1058
Kzo9I GATC 6 cut(s) 438, 610, 643, 763, 1513, 1593
LguI GCTCTTC 4 cut(s) 198, 303, 678, 1008
Lsp1109I GCAGC 5 cut(s) 71, 215, 546, 1333, 1336
LweI GCATC 1 cut(s) 145
MaeI CTAG 3 cut(s) 96, 872, 1520
MaeII ACGT 3 cut(s) 567, 678, 1453
MaeIII GTNAC 3 cut(s) 383, 994, 1390
MalI GATC 6 cut(s) 440, 612, 645, 765, 1515, 1595
MboI GATC 6 cut(s) 438, 610, 643, 763, 1513, 1593
MflI RGATCY 4 cut(s) 610, 643, 1513, 1593
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 9 cut(s) 71, 176, 408, 515, 570, 716, 778, 941, 1480
Mly113I GGCGCC 2 cut(s) 376, 1239
MnlI CCTC 4 cut(s) 353, 803, 906, 1527
Mph1103I ATGCAT 1 cut(s) 1561
MseI TTAA 8 cut(s) 41, 336, 407, 461, 549, 782, 1230, 1606
MslI CAYNNNNRTG 1 cut(s) 696
MspA1I CMGCKG 2 cut(s) 62, 1057
MspR9I CCNGG 3 cut(s) 27, 208, 1354
Mva1269I GAATGC 5 cut(s) 130, 308, 883, 1305, 1559
MvaI CCWGG 3 cut(s) 27, 208, 1354
MvnI CGCG 2 cut(s) 1057, 1094
MwoI GCNNNNNNNGC 4 cut(s) 164, 635, 1187, 1556
NarI GGCGCC 2 cut(s) 376, 1239
NcoI CCATGG 1 cut(s) 379
NdeII GATC 6 cut(s) 438, 610, 643, 763, 1513, 1593
NlaIV GGNNCC 4 cut(s) 218, 377, 948, 1240
NmuCI GTSAC 1 cut(s) 1390
NsiI ATGCAT 1 cut(s) 1561
NspI RCATGY 2 cut(s) 701, 1527
NspV TTCGAA 1 cut(s) 972
PagI TCATGA 3 cut(s) 301, 312, 1017
PciSI GCTCTTC 4 cut(s) 198, 303, 678, 1008
PctI GAATGC 5 cut(s) 130, 308, 883, 1305, 1559
PfeI GAWTC 1 cut(s) 1220
Pfl23II CGTACG 1 cut(s) 564
PflMI CCANNNNNTGG 2 cut(s) 653, 1581
PkrI GCNGC 5 cut(s) 61, 205, 561, 1348, 1351
PluTI GGCGCC 2 cut(s) 379, 1242
Ppu21I YACGTR 2 cut(s) 568, 679
PshAI GACNNNNGTC 1 cut(s) 1095
PshBI ATTAAT 2 cut(s) 41, 407
Psp6I CCWGG 3 cut(s) 25, 206, 1352
PspGI CCWGG 3 cut(s) 25, 206, 1352
PspLI CGTACG 1 cut(s) 564
PspN4I GGNNCC 4 cut(s) 218, 377, 948, 1240
PstI CTGCAG 1 cut(s) 1552
PsuI RGATCY 4 cut(s) 610, 643, 1513, 1593
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 3 cut(s) 566, 827, 948
RsaNI GTAC 3 cut(s) 565, 826, 947
RseI CAYNNNNRTG 1 cut(s) 696
SacII CCGCGG 1 cut(s) 1058
SapI GCTCTTC 4 cut(s) 198, 303, 678, 1008
SaqAI TTAA 8 cut(s) 41, 336, 407, 461, 549, 782, 1230, 1606
SatI GCNGC 5 cut(s) 60, 204, 560, 1347, 1350
Sau3AI GATC 6 cut(s) 438, 610, 643, 763, 1513, 1593
ScrFI CCNGG 3 cut(s) 27, 208, 1354
SduI GDGCHC 1 cut(s) 219
SfaNI GCATC 1 cut(s) 145
SfcI CTRYAG 4 cut(s) 622, 1209, 1487, 1548
SfoI GGCGCC 2 cut(s) 377, 1240
Sfr303I CCGCGG 1 cut(s) 1058
SfuI TTCGAA 1 cut(s) 972
SgrBI CCGCGG 1 cut(s) 1058
SmiI ATTTAAAT 1 cut(s) 783
SmiMI CAYNNNNRTG 1 cut(s) 696
SnaBI TACGTA 1 cut(s) 568
Sse9I AATT 9 cut(s) 71, 176, 408, 515, 570, 716, 778, 941, 1480
SsiI CCGC 5 cut(s) 532, 1055, 1057, 1094, 1413
SspDI GGCGCC 2 cut(s) 375, 1238
SspMI CTAG 3 cut(s) 96, 872, 1520
StyD4I CCNGG 3 cut(s) 25, 206, 1352
StyI CCWWGG 1 cut(s) 379
SwaI ATTTAAAT 1 cut(s) 783
TaaI ACNGT 4 cut(s) 334, 391, 430, 1604
TaiI ACGT 3 cut(s) 570, 681, 1456
TaqI TCGA 5 cut(s) 75, 813, 953, 972, 1401
TasI AATT 9 cut(s) 71, 176, 408, 515, 570, 716, 778, 941, 1480
TatI WGTACW 1 cut(s) 825
TfiI GAWTC 1 cut(s) 1220
Tru1I TTAA 8 cut(s) 41, 336, 407, 461, 549, 782, 1230, 1606
Tru9I TTAA 8 cut(s) 41, 336, 407, 461, 549, 782, 1230, 1606
TscAI CASTG 3 cut(s) 337, 433, 802
TseFI GTSAC 1 cut(s) 1390
TseI GCWGC 5 cut(s) 59, 203, 559, 1346, 1349
Tsp45I GTSAC 1 cut(s) 1390
TspDTI ATGAA 7 cut(s) 185, 290, 301, 318, 329, 479, 1034
TspRI CASTG 3 cut(s) 337, 433, 802
Van91I CCANNNNNTGG 2 cut(s) 653, 1581
VspI ATTAAT 2 cut(s) 41, 407
XagI CCTNNNNNAGG 1 cut(s) 771
XapI RAATTY 3 cut(s) 71, 176, 1480
XceI RCATGY 2 cut(s) 701, 1527
XspI CTAG 3 cut(s) 96, 872, 1520
Zsp2I ATGCAT 1 cut(s) 1561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.