RchiOBHm_Chr7g0242411

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0021465
Physical Location & Seq
Reverse (-)
68519769 .. 68521553
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21726

Sequence Viewer

Length: 1587 bp
ATGTCAAGTCTTCTTGCAGCTGAAAACGATGCCAAGGAAATCGAGCGCTACATACCCTTCTCTAACTTTGTGATAAATGGTGATTGGGAGAATGCAAAGGAGTGTCTTACAAAACTTCCAGATGCAATAACAGCAAGATACCAATTTGGAGCCACAGCCCTTCATATAGCAGCCGGGCAAGGGCACCTGCATATCTTGAAAGAGTTGGTCTTGCTGATGGGACAAGATGATTTACAAACTAAAAACGCTTATGGTTACACAGCTCTTCATGTAGCATTCTTCAACGGGCGCTTTGACATTGTTAAAGAGCTGTTGCCCCTGATGGGAGGAGTAAAAGACTGCCATGGTAACACGGTTCTTCACATGGCATTAATTAGGCAGCAACCACACACTGTAGAGTGGATGGTGAAGTTAATGAAAAGAGAAGACTTGGAATTAAAAAACAACGATGGTTCTACAGCTCTGAACATAGCAGTCAAGAAGGGGAATGTGTCTACTATTAAAGAGTTGCTGCCGCATGATGATGACTTTGACCGTTATATACCGTTTACTAATGCTGTAATTGACGGTGATTGGAATAATGCAAACTATTGTCTTAGAGATCTAGATCCCTACAGGGCAGTAAGAGCAAGAGATCCAAGAGATGGAAATGAGAACACGGCTCTTCACTTAGCAATCAAGCATGGGCATGTGGATATTGTGAAAGAGTTGGTGTCGTTGATGACACAAGAAGATTTGGAAGTGAAAAATGCTGATCCTTCCACGGGTAATTTAAATGCAGACCTCAGTGAACGAGCAGTCGTCGAAATGGCTAAGTACATGGTTGAACAGAATGAGATACTGCTTGCCCGTGTTTTAGAACTAGAGAATGCTTTTGGAGATACACCTCTTCACGAAGCAGTCAGTAAGGGGGATATGTCTATTGTGGAAGAATTGGTACCTTCGATGAGACAAGAAGGTTTTGAAATAAAAAATAATAATGGTTTTACAGCTCTTCACCAAGCAGTCATGAACGAAAATGTGGATATTGTGAAGATTATAGTCCGGCGGATGAGGCGAGAAGGTCTGGAAGTAAAAGGTGCTCTTGGTTACACAGCACTTCACCAAGCAGTGGAGATGGGGAATATGGGGATTGTGAAAGAGTTGGTATTGGTGATGAGACAAGAGGGTCTTGAAGTAAAAAACGACGCGGGTTCCAATGCTCTTCACCTTGCAGTCATGGAGAATAAGAATATGGACATTGTGAGAGAGTTGATGTCGTTGATGAGACCACAAGCGATGGAAATAAAAAATGGTGAAGGTTTTACAGCTTTAGGTTGTGCTATGGAGCTACCCGCCATAGATGGAGACATTTGGGAAATGGCTAAATTCATGGCTGGAAAGAACAAAAAAGTATTTGGCATGCGCTCGGGTAGAGACATTCCAGTTGTATGGGCTGCTGGCTGGGGCAAGTGGAAATTAACTCAGTATCTCTATTTCGTCACTCCTCGTGAAGCTCTAAACGGGTTTGACGGCGCTCATTTTATTACTATTGCTATTGAACGTCTGAAAGCGCTCGGTAAGGGTGTTTTAAATATCATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

528

Amino Acids

59.02

Weight (kDa)

5.59

Isoelectric Point (pI)

25.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 42 - 106 2.3e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 54 - 102 7.7e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 111 - 178 5.9e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 287 - 348 2.5e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 323 - 383 6.1e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 361 - 420 1.1e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 367 - 443 3.5e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021465)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0242411 RchiOBHm_Chr7g0242471
rosa_multiflora Rmu_sc0000429.1_g000058 Rmu_sc0000429.1_g000066
rosa_rugosa Rorug04G0006800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 195
AasI GACNNNNNNGTC 1 cut(s) 1167
Acc36I ACCTGC 1 cut(s) 195
Acc65I GGTACC 1 cut(s) 937
AccB1I GGYRCC 2 cut(s) 183, 937
AccB7I CCANNNNNTGG 2 cut(s) 644, 1111
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 1190
AciI CCGC 4 cut(s) 515, 1048, 1190, 1335
AclWI GGATC 3 cut(s) 602, 629, 749
AcsI RAATTY 1 cut(s) 1367
AfaI GTAC 2 cut(s) 818, 939
AfeI AGCGCT 2 cut(s) 47, 1554
AfiI CCNNNNNNNGG 5 cut(s) 180, 323, 644, 764, 1111
AgsI TTSAA 6 cut(s) 199, 283, 827, 965, 1175, 1541
AluBI AGCT 8 cut(s) 20, 263, 310, 461, 992, 1310, 1330, 1496
AluI AGCT 8 cut(s) 20, 263, 310, 461, 992, 1310, 1330, 1496
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 5 cut(s) 943, 1153, 1261, 1341, 1410
AlwI GGATC 3 cut(s) 602, 629, 749
Ama87I CYCGRG 1 cut(s) 1408
Aor51HI AGCGCT 2 cut(s) 47, 1554
ApeKI GCWGC 5 cut(s) 17, 170, 379, 511, 1436
ApoI RAATTY 1 cut(s) 1367
AseI ATTAAT 2 cut(s) 371, 1581
Asp718I GGTACC 1 cut(s) 937
AspLEI GCGC 5 cut(s) 48, 291, 1407, 1517, 1555
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 8 cut(s) 92, 418, 581, 989, 1094, 1165, 1199, 1307
AvaI CYCGRG 1 cut(s) 1408
BaeGI GKGCMC 1 cut(s) 186
BanI GGYRCC 2 cut(s) 183, 937
BarI GAAGNNNNNNTAC 2 cut(s) 921, 953
BauI CACGAG 1 cut(s) 1488
BbsI GAAGAC 2 cut(s) 2, 432
Bbv12I GWGCWC 1 cut(s) 1084
BbvI GCAGC 5 cut(s) 29, 182, 391, 498, 1423
BccI CCATC 8 cut(s) 211, 316, 397, 443, 638, 1111, 1273, 1337
BceAI ACGGC 2 cut(s) 675, 1528
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 5 cut(s) 943, 1153, 1261, 1341, 1410
BfaI CTAG 2 cut(s) 605, 863
BfmI CTRYAG 3 cut(s) 393, 456, 613
BfoI RGCGCY 4 cut(s) 49, 292, 1518, 1556
BfuAI ACCTGC 1 cut(s) 195
BglII AGATCT 1 cut(s) 601
BisI GCNGC 6 cut(s) 18, 171, 380, 512, 515, 1437
BlsI GCNGC 6 cut(s) 19, 172, 381, 513, 516, 1438
Bme1390I CCNGG 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 1408
BmiI GGNNCC 4 cut(s) 151, 185, 939, 1195
BmrFI CCNGG 1 cut(s) 175
BmsI GCATC 2 cut(s) 19, 112
BpiI GAAGAC 2 cut(s) 2, 432
BpuMI CCSGG 1 cut(s) 175
BsaBI GATNNNNATC 1 cut(s) 606
BsaI GGTCTC 1 cut(s) 1261
BsaJI CCNNGG 3 cut(s) 33, 343, 762
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 323, 644, 764, 1111
Bse1I ACTGG 1 cut(s) 1424
Bse8I GATNNNNATC 1 cut(s) 606
BseDI CCNNGG 3 cut(s) 33, 343, 762
BseGI GGATG 2 cut(s) 408, 1056
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 5 cut(s) 180, 323, 644, 764, 1111
BseMII CTCAG 2 cut(s) 799, 1478
BseNI ACTGG 1 cut(s) 1424
BseRI GAGGAG 2 cut(s) 342, 1476
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 5 cut(s) 29, 182, 391, 498, 1423
BseYI CCCAGC 1 cut(s) 1443
Bsh1236I CGCG 1 cut(s) 1190
BshNI GGYRCC 2 cut(s) 183, 937
BsiHKAI GWGCWC 1 cut(s) 1084
BsiHKCI CYCGRG 1 cut(s) 1408
BsiSI CCGG 2 cut(s) 174, 1045
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 5 cut(s) 180, 323, 644, 764, 1111
BsmAI GTCTC 5 cut(s) 943, 1153, 1261, 1341, 1410
BsmFI GGGAC 1 cut(s) 234
BsmI GAATGC 3 cut(s) 97, 275, 874
Bso31I GGTCTC 1 cut(s) 1261
BsoBI CYCGRG 1 cut(s) 1408
Bsp1286I GDGCHC 2 cut(s) 186, 1084
Bsp143I GATC 4 cut(s) 601, 607, 634, 754
Bsp19I CCATGG 1 cut(s) 343
BspACI CCGC 4 cut(s) 515, 1048, 1190, 1335
BspCNI CTCAG 2 cut(s) 798, 1477
BspFNI CGCG 1 cut(s) 1190
BspHI TCATGA 1 cut(s) 1008
BspLI GGNNCC 4 cut(s) 151, 185, 939, 1195
BspMI ACCTGC 1 cut(s) 195
BspPI GGATC 3 cut(s) 602, 629, 749
BspQI GCTCTTC 4 cut(s) 270, 669, 999, 1209
BspT107I GGYRCC 2 cut(s) 183, 937
BspTNI GGTCTC 1 cut(s) 1261
BsrI ACTGG 1 cut(s) 1424
BssECI CCNNGG 3 cut(s) 33, 343, 762
BssMI GATC 4 cut(s) 601, 607, 634, 754
BssSI CACGAG 1 cut(s) 1488
BssT1I CCWWGG 2 cut(s) 33, 343
Bst2BI CACGAG 1 cut(s) 1488
Bst4CI ACNGT 5 cut(s) 355, 394, 536, 546, 569
Bst6I CTCTTC 5 cut(s) 270, 669, 894, 999, 1209
BstC8I GCNNGC 3 cut(s) 846, 1403, 1441
BstDEI CTNAG 5 cut(s) 596, 670, 785, 813, 1464
BstDSI CCRYGG 2 cut(s) 343, 762
BstF5I GGATG 2 cut(s) 408, 1056
BstFNI CGCG 1 cut(s) 1190
BstH2I RGCGCY 4 cut(s) 49, 292, 1518, 1556
BstHHI GCGC 5 cut(s) 48, 291, 1407, 1517, 1555
BstKTI GATC 4 cut(s) 604, 610, 637, 757
BstMAI GTCTC 5 cut(s) 943, 1153, 1261, 1341, 1410
BstMBI GATC 4 cut(s) 601, 607, 634, 754
BstMWI GCNNNNNNNGC 3 cut(s) 131, 626, 1054
BstNSI RCATGY 2 cut(s) 692, 1405
BstSCI CCNGG 1 cut(s) 173
BstSFI CTRYAG 3 cut(s) 393, 456, 613
BstSLI GKGCMC 1 cut(s) 186
BstUI CGCG 1 cut(s) 1190
BstV1I GCAGC 5 cut(s) 29, 182, 391, 498, 1423
BstV2I GAAGAC 2 cut(s) 2, 432
BstX2I RGATCY 3 cut(s) 601, 607, 634
BstXI CCANNNNNNTGG 1 cut(s) 1431
BstYI RGATCY 3 cut(s) 601, 607, 634
BtgI CCRYGG 2 cut(s) 343, 762
BtgZI GCGATG 1 cut(s) 1292
BtsCI GGATG 2 cut(s) 408, 1056
BtsI GCAGTG 1 cut(s) 1116
BtsIMutI CAGTG 3 cut(s) 390, 793, 1116
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 3 cut(s) 846, 1403, 1441
CciI TCATGA 1 cut(s) 1008
CfoI GCGC 5 cut(s) 48, 291, 1407, 1517, 1555
CseI GACGC 1 cut(s) 1196
Csp6I GTAC 2 cut(s) 817, 938
CviQI GTAC 2 cut(s) 817, 938
DdeI CTNAG 5 cut(s) 596, 670, 785, 813, 1464
DpnI GATC 4 cut(s) 603, 609, 636, 756
DpnII GATC 4 cut(s) 601, 607, 634, 754
DraI TTTAAA 2 cut(s) 774, 1572
DrdI GACNNNNNNGTC 1 cut(s) 1167
DseDI GACNNNNNNGTC 1 cut(s) 1167
Eam1104I CTCTTC 5 cut(s) 270, 669, 894, 999, 1209
EarI CTCTTC 5 cut(s) 270, 669, 894, 999, 1209
EciI GGCGGA 1 cut(s) 1063
Eco130I CCWWGG 2 cut(s) 33, 343
Eco31I GGTCTC 1 cut(s) 1261
Eco47III AGCGCT 2 cut(s) 47, 1554
Eco88I CYCGRG 1 cut(s) 1408
EcoT14I CCWWGG 2 cut(s) 33, 343
ErhI CCWWGG 2 cut(s) 33, 343
FalI AAGNNNNNCTT 4 cut(s) 1068, 1100, 1155, 1187
FaqI GGGAC 1 cut(s) 234
FauI CCCGC 2 cut(s) 1183, 1342
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 6 cut(s) 18, 171, 380, 512, 515, 1437
FokI GGATG 2 cut(s) 415, 1063
Fsp4HI GCNGC 6 cut(s) 18, 171, 380, 512, 515, 1437
FspBI CTAG 2 cut(s) 605, 863
GlaI GCGC 5 cut(s) 47, 290, 1406, 1516, 1554
GluI GCNGC 6 cut(s) 18, 171, 380, 512, 515, 1437
GsaI CCCAGC 1 cut(s) 1447
HaeII RGCGCY 4 cut(s) 49, 292, 1518, 1556
HapII CCGG 2 cut(s) 174, 1045
HgaI GACGC 1 cut(s) 1196
HhaI GCGC 5 cut(s) 48, 291, 1407, 1517, 1555
Hin6I GCGC 5 cut(s) 46, 289, 1405, 1515, 1553
HinP1I GCGC 5 cut(s) 46, 289, 1405, 1515, 1553
HpaII CCGG 2 cut(s) 174, 1045
HphI GGTGA 8 cut(s) 92, 418, 581, 989, 1094, 1165, 1199, 1307
Hpy166II GTNNAC 3 cut(s) 495, 549, 791
Hpy188I TCNGA 2 cut(s) 465, 1548
Hpy188III TCNNGA 9 cut(s) 119, 196, 478, 605, 893, 1009, 1067, 1172, 1490
Hpy8I GTNNAC 3 cut(s) 495, 549, 791
Hpy99I CGWCG 2 cut(s) 806, 1190
HpyAV CCTTC 8 cut(s) 67, 170, 475, 768, 950, 951, 1055, 1292
HpyCH4III ACNGT 5 cut(s) 355, 394, 536, 546, 569
HpyCH4IV ACGT 1 cut(s) 1543
HpyCH4V TGCA 7 cut(s) 17, 95, 125, 190, 584, 779, 1214
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 626, 1054
HpyF3I CTNAG 5 cut(s) 596, 670, 785, 813, 1464
HpySE526I ACGT 1 cut(s) 1543
HspAI GCGC 5 cut(s) 46, 289, 1405, 1515, 1553
KpnI GGTACC 1 cut(s) 941
Kzo9I GATC 4 cut(s) 601, 607, 634, 754
LguI GCTCTTC 4 cut(s) 270, 669, 999, 1209
LmnI GCTCC 2 cut(s) 149, 1327
Lsp1109I GCAGC 5 cut(s) 29, 182, 391, 498, 1423
LweI GCATC 2 cut(s) 19, 112
MaeI CTAG 2 cut(s) 605, 863
MaeII ACGT 1 cut(s) 1543
MaeIII GTNAC 4 cut(s) 254, 347, 1088, 1480
MalI GATC 4 cut(s) 603, 609, 636, 756
MboI GATC 4 cut(s) 601, 607, 634, 754
MflI RGATCY 3 cut(s) 601, 607, 634
MhlI GDGCHC 2 cut(s) 186, 1084
MluCI AATT 9 cut(s) 143, 372, 434, 561, 769, 932, 1367, 1457, 1582
MnlI CCTC 6 cut(s) 320, 794, 897, 1047, 1159, 1497
MslI CAYNNNNRTG 2 cut(s) 522, 687
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 2 cut(s) 174, 1045
MspR9I CCNGG 1 cut(s) 175
Mva1269I GAATGC 3 cut(s) 97, 275, 874
MvnI CGCG 1 cut(s) 1190
MwoI GCNNNNNNNGC 3 cut(s) 131, 626, 1054
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 343
NdeII GATC 4 cut(s) 601, 607, 634, 754
NlaIV GGNNCC 4 cut(s) 151, 185, 939, 1195
NmuCI GTSAC 1 cut(s) 1480
NspI RCATGY 2 cut(s) 692, 1405
PacI TTAATTAA 1 cut(s) 1585
PaeI GCATGC 1 cut(s) 1405
PagI TCATGA 1 cut(s) 1008
PaqCI CACCTGC 1 cut(s) 195
PciSI GCTCTTC 4 cut(s) 270, 669, 999, 1209
PctI GAATGC 3 cut(s) 97, 275, 874
PflMI CCANNNNNTGG 2 cut(s) 644, 1111
PkrI GCNGC 6 cut(s) 19, 172, 381, 513, 516, 1438
PshBI ATTAAT 2 cut(s) 371, 1581
PspFI CCCAGC 1 cut(s) 1443
PspN4I GGNNCC 4 cut(s) 151, 185, 939, 1195
PsuI RGATCY 3 cut(s) 601, 607, 634
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 2 cut(s) 818, 939
RsaNI GTAC 2 cut(s) 817, 938
RseI CAYNNNNRTG 2 cut(s) 522, 687
SapI GCTCTTC 4 cut(s) 270, 669, 999, 1209
SatI GCNGC 6 cut(s) 18, 171, 380, 512, 515, 1437
Sau3AI GATC 4 cut(s) 601, 607, 634, 754
ScrFI CCNGG 1 cut(s) 175
SduI GDGCHC 2 cut(s) 186, 1084
SfaNI GCATC 2 cut(s) 19, 112
SfcI CTRYAG 3 cut(s) 393, 456, 613
SmiI ATTTAAAT 1 cut(s) 774
SmiMI CAYNNNNRTG 2 cut(s) 522, 687
SphI GCATGC 1 cut(s) 1405
Sse9I AATT 9 cut(s) 143, 372, 434, 561, 769, 932, 1367, 1457, 1582
SsiI CCGC 4 cut(s) 515, 1048, 1190, 1335
SspMI CTAG 2 cut(s) 605, 863
StyD4I CCNGG 1 cut(s) 173
StyI CCWWGG 2 cut(s) 33, 343
SwaI ATTTAAAT 1 cut(s) 774
TaaI ACNGT 5 cut(s) 355, 394, 536, 546, 569
TaiI ACGT 1 cut(s) 1546
TaqI TCGA 3 cut(s) 42, 804, 944
TasI AATT 9 cut(s) 143, 372, 434, 561, 769, 932, 1367, 1457, 1582
TatI WGTACW 1 cut(s) 816
TauI GCSGC 1 cut(s) 517
TscAI CASTG 3 cut(s) 397, 793, 1116
TseFI GTSAC 1 cut(s) 1480
TseI GCWGC 5 cut(s) 17, 170, 379, 511, 1436
Tsp45I GTSAC 1 cut(s) 1480
TspDTI ATGAA 5 cut(s) 152, 257, 431, 1025, 1360
TspRI CASTG 3 cut(s) 397, 793, 1116
Van91I CCANNNNNTGG 2 cut(s) 644, 1111
VspI ATTAAT 2 cut(s) 371, 1581
XapI RAATTY 1 cut(s) 1367
XbaI TCTAGA 1 cut(s) 604
XceI RCATGY 2 cut(s) 692, 1405
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 2 cut(s) 605, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.