RchiOBHm_Chr5g0004841

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013815
Physical Location & Seq
Reverse (-)
3197541 .. 3200481
2941 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGGCCACCAAAGTCTACATTGTTTACTATTCTATGTATGGACATGTTGAGAAGCTGGCTGAAGAGATTAAAAAGGGAGCTGCATCTGTGGAAGGAGTAGAAGCCCAACTATATCAGGTACCAGAAACACTGCAAGATGAGGTTCTCGGAAAGATGGGTGCACCACCAAAGAGCGAAGTACCTATTATTGCACCCAATGAGCTTTCTGATGCCGATGGTTTACTATTTGGTTTCCCTACTAGATTTGGAATGATGGCTGCACAGTTCAAAGCATTTTTAGATGCAACCGGAGGTTTATGGAGGACACAACAGTTAGCAGGCAAACCTGCAGGAATTTTCTACAGCACTGGTTCTCAAGGAGGTGGACAAGAGACTACCGCCTTGACAGCCATCACTCAGCTTGTTCACCATGGAATGGTGTTTGTGCCAATTGGGTACACATTTGGAGCTGGCATGTTTGAGATGGAGCAGATCAAGGGTGGCAGCCCCTATGGTGCAGGAACTTATGCCGGGGATGGCACAAGACAACCTTCTGAACTCGAATTACAGCAAGCTTTCCACCAGGGAAAGTATTTCGCCAGCATTGCCAAGAAACTCAAGGGGACTACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

204

Amino Acids

21.79

Weight (kDa)

5.81

Isoelectric Point (pI)

32.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavodoxin_2 PF02525 4 - 128 9.2e-06 Flavodoxin-like fold
Flavodoxin_1 PF00258 7 - 138 5.2e-10 Flavodoxin
FMN_red PF03358 17 - 145 3.1e-13 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013815)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54500
fragaria_vesca FvH4_3g03210 FvH4_3g03210
rosa_chinensis RchiOBHm_Chr5g0004841
rosa_laevigata RLG00000031266
rosa_multiflora Rmu_sc0010871.1_g000002
rosa_roxburghii Rroxscaffold_1G00070770
rosa_rugosa Rorug04G0414400 Rorug04G0414400 Rorug04G0414500 Rorug04G0414600 Rorug04G0414700.1
rosa_samantha Rh5AG042200 Rh5BG041900 Rh5CG045700 Rh5DG042300
rosa_wichuraiana Rw5G004080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 334
Acc65I GGTACC 1 cut(s) 118
AccB1I GGYRCC 1 cut(s) 118
AccB7I CCANNNNNTGG 1 cut(s) 415
AccI GTMKAC 1 cut(s) 15
AciI CCGC 1 cut(s) 378
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 333
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 3 cut(s) 120, 180, 437
AfiI CCNNNNNNNGG 1 cut(s) 415
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 1 cut(s) 268
AjnI CCWGG 1 cut(s) 561
AluBI AGCT 6 cut(s) 55, 80, 202, 400, 449, 554
AluI AGCT 6 cut(s) 55, 80, 202, 400, 449, 554
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 1 cut(s) 365
Alw44I GTGCAC 1 cut(s) 159
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 159
ApeKI GCWGC 3 cut(s) 80, 257, 483
ApoI RAATTY 1 cut(s) 333
Asp718I GGTACC 1 cut(s) 118
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 163
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 118
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 3 cut(s) 67, 244, 495
BccI CCATC 6 cut(s) 148, 209, 247, 398, 457, 509
BciT130I CCWGG 1 cut(s) 563
BcnI CCSGG 1 cut(s) 511
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 1 cut(s) 240
BfmI CTRYAG 2 cut(s) 327, 340
BfuAI ACCTGC 1 cut(s) 334
BisI GCNGC 3 cut(s) 81, 258, 484
BlsI GCNGC 3 cut(s) 82, 259, 485
Bme1390I CCNGG 2 cut(s) 511, 563
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 2 cut(s) 511, 563
BmsI GCATC 3 cut(s) 92, 199, 271
BpuEI CTTGAG 2 cut(s) 339, 581
BpuMI CCSGG 1 cut(s) 511
BsaJI CCNNGG 3 cut(s) 409, 510, 562
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 582
BseBI CCWGG 1 cut(s) 563
BseDI CCNNGG 3 cut(s) 409, 510, 562
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 1 cut(s) 410
BseNI ACTGG 1 cut(s) 352
BseSI GKGCMC 1 cut(s) 163
BseXI GCAGC 3 cut(s) 67, 244, 495
BsgI GTGCAG 2 cut(s) 243, 516
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 2 cut(s) 288, 510
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 1 cut(s) 365
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 1 cut(s) 471
Bsp19I CCATGG 1 cut(s) 409
BspACI CCGC 1 cut(s) 378
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 409
BspLI GGNNCC 1 cut(s) 120
BspMAI CTGCAG 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 334
BspT107I GGYRCC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 582
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 3 cut(s) 409, 510, 562
BssMI GATC 1 cut(s) 471
BssT1I CCWWGG 1 cut(s) 409
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 2 cut(s) 264, 312
Bst6I CTCTTC 1 cut(s) 57
BstC8I GCNNGC 5 cut(s) 57, 319, 451, 552, 580
BstDEI CTNAG 1 cut(s) 396
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 1 cut(s) 520
BstKTI GATC 1 cut(s) 474
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 1 cut(s) 471
BstMWI GCNNNNNNNGC 2 cut(s) 386, 584
BstNI CCWGG 1 cut(s) 563
BstNSI RCATGY 2 cut(s) 47, 457
BstSCI CCNGG 2 cut(s) 509, 561
BstSFI CTRYAG 2 cut(s) 327, 340
BstSLI GKGCMC 1 cut(s) 163
BstV1I GCAGC 3 cut(s) 67, 244, 495
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 2 cut(s) 128, 345
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 5 cut(s) 57, 319, 451, 552, 580
Csp6I GTAC 3 cut(s) 119, 179, 436
CviAII CATG 3 cut(s) 44, 410, 454
CviQI GTAC 3 cut(s) 119, 179, 436
DdeI CTNAG 1 cut(s) 396
DpnI GATC 1 cut(s) 473
DpnII GATC 1 cut(s) 471
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 561
EcoT14I CCWWGG 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 3 cut(s) 47, 413, 457
FaiI YATR 9 cut(s) 35, 39, 45, 112, 298, 411, 455, 492, 507
FalI AAGNNNNNCTT 2 cut(s) 514, 546
FatI CATG 3 cut(s) 43, 409, 453
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 3 cut(s) 81, 258, 484
FokI GGATG 1 cut(s) 527
Fsp4HI GCNGC 3 cut(s) 81, 258, 484
FspBI CTAG 1 cut(s) 240
GluI GCNGC 3 cut(s) 81, 258, 484
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 288, 510
Hin1II CATG 3 cut(s) 47, 413, 457
HindIII AAGCTT 1 cut(s) 552
HpaII CCGG 2 cut(s) 288, 510
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 7 cut(s) 16, 25, 161, 221, 365, 406, 438
Hpy188I TCNGA 3 cut(s) 149, 208, 535
Hpy8I GTNNAC 7 cut(s) 16, 25, 161, 221, 365, 406, 438
HpyAV CCTTC 2 cut(s) 86, 540
HpyCH4III ACNGT 2 cut(s) 264, 312
HpyCH4V TGCA 8 cut(s) 83, 133, 161, 191, 260, 284, 329, 497
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 584
HpyF3I CTNAG 1 cut(s) 396
Hsp92II CATG 3 cut(s) 47, 413, 457
KpnI GGTACC 1 cut(s) 122
Kzo9I GATC 1 cut(s) 471
LmnI GCTCC 3 cut(s) 77, 446, 466
Lsp1109I GCAGC 3 cut(s) 67, 244, 495
LweI GCATC 3 cut(s) 92, 199, 271
MaeI CTAG 1 cut(s) 240
MalI GATC 1 cut(s) 473
MboI GATC 1 cut(s) 471
MboII GAAGA 1 cut(s) 74
MfeI CAATTG 1 cut(s) 429
MhlI GDGCHC 1 cut(s) 163
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 333, 429, 542
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 4 cut(s) 133, 284, 294, 353
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 69
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 288, 510
MspR9I CCNGG 2 cut(s) 511, 563
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 563
MwoI GCNNNNNNNGC 2 cut(s) 386, 584
NciI CCSGG 1 cut(s) 511
NcoI CCATGG 1 cut(s) 409
NdeII GATC 1 cut(s) 471
NlaIII CATG 3 cut(s) 47, 413, 457
NlaIV GGNNCC 1 cut(s) 120
NspI RCATGY 2 cut(s) 47, 457
PciI ACATGT 1 cut(s) 43
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 3 cut(s) 82, 259, 485
PscI ACATGT 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 561
PspGI CCWGG 1 cut(s) 561
PspN4I GGNNCC 1 cut(s) 120
PsrI GAACNNNNNNTAC 2 cut(s) 528, 560
PstI CTGCAG 1 cut(s) 331
RsaI GTAC 3 cut(s) 120, 180, 437
RsaNI GTAC 3 cut(s) 119, 179, 436
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 3 cut(s) 81, 258, 484
Sau3AI GATC 1 cut(s) 471
SbfI CCTGCAGG 1 cut(s) 331
ScrFI CCNGG 2 cut(s) 511, 563
SdaI CCTGCAGG 1 cut(s) 331
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 3 cut(s) 92, 199, 271
SfcI CTRYAG 2 cut(s) 327, 340
SmlI CTYRAG 2 cut(s) 354, 596
SmoI CTYRAG 2 cut(s) 354, 596
Sse8387I CCTGCAGG 1 cut(s) 331
Sse9I AATT 3 cut(s) 333, 429, 542
SsiI CCGC 1 cut(s) 378
SspMI CTAG 1 cut(s) 240
StyD4I CCNGG 2 cut(s) 509, 561
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 264, 312
TaqI TCGA 1 cut(s) 540
TasI AATT 3 cut(s) 333, 429, 542
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 2 cut(s) 135, 352
TseI GCWGC 3 cut(s) 80, 257, 483
TspRI CASTG 2 cut(s) 135, 352
Van91I CCANNNNNTGG 1 cut(s) 415
VneI GTGCAC 1 cut(s) 159
XapI RAATTY 1 cut(s) 333
XceI RCATGY 2 cut(s) 47, 457
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.