RchiOBHm_Chr5g0028421

Cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018400
Physical Location & Seq
Reverse (-)
22256655 .. 22260863
4209 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGAATAGATCTATGCAAGTTGATGAAGGGTTAATGATCATAGAAGAGGCAATTCAGAAAGCAAAGAAGATTGTGGAAGGATTCCCAGAGACAAAGTTCACCACGGAAGAATATATGAAGTACTATGCATGTGTTTATATTATGTGCGATAAGCCTGGGGCAGGAAATGAGGCGTTGCTATATGAGAAATTTAAGAACAGCTTGGAAGACATCATCAGCTCCTTGGCACTGCCCTCGTTGGCAGCGAAAGAAAATGAGCTTCTGTTAAGGGAACTTGTACAGATGTGGTCAAATTACAAGATAATGGCAAGATGGCTATGTAGATTCTTTGAATACTTGGACCGCTATTATATCCCCCGTCATTGTCTTGTTTCACTTACTGACACCTCAATTGATTGCTTTTGTAATCTGGTCTTCAATGGGTTGGCTAACAGAATTCAGGCAGCTGCAATGTCTCTGATTAATCAAGAGCGTTATGGACAACTGATTGATGCAAAGATGCTTCAAGATGTCATGAGTATCTTTATGGAAATTGGTCAAAATAGATCACCATCATACTACGAAATCATTGAGCAGGCTGTGCTCGGAGAAACTGCCACTTTCTATTCCCAATTATCTTCACAGTGGCTTTTGTGCGATTCGTTGACTGATTATATCCGGAAGGTGAACTTGTGCATTTTGCAGGAGAAAGAAAGAGCTGGCCGGTATCTACATCCCACATCTGTGGTGAAGTTAATACAGGTTGTGCGATTCCAGTTGCTGGATCTGAATGCAAATAAGTTGCTTGAAAAGCAGAAGTCTGAAAACTGCAGTCAACTGGATTATCAGGAGATATTGTCAAAGTGTGCCTGCTTAAATCTTGGAGAGGACAGCTCTACATCAACACTAGTGGAAAATCTCTGTTCTTCAATGGCACAATCAGCTCATATTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04114 ko04120 ko04141 ko04310 ko04340 ko04341 ko04350 ko04710 ko05168 ko05200 map04110 map04111 map04114 map04120 map04141 map04310 map04340 map04341 map04350 map04710 map05168 map05200
Pfam Domains
Protein Families
Cullin

Protein Analysis

311

Amino Acids

35.77

Weight (kDa)

5.22

Isoelectric Point (pI)

52.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 30 - 271 1.1e-44 Cullin alpha solenoid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018400)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g17070 FvH4_3g17070 FvH4_3g17070
rosa_chinensis RchiOBHm_Chr5g0028421
rosa_laevigata RLG00000033093
rosa_multiflora Rmu_sc0006771.1_g000005
rosa_roxburghii Rroxscaffold_1G00051260
rosa_rugosa Rorug05G0107500
rosa_samantha Rh5AG200300 Rh5BG198700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 760
AccIII TCCGGA 1 cut(s) 657
AciI CCGC 1 cut(s) 343
AclWI GGATC 1 cut(s) 771
AcoI YGGCCR 1 cut(s) 700
AcsI RAATTY 2 cut(s) 188, 435
AfaI GTAC 2 cut(s) 122, 279
AfiI CCNNNNNNNGG 2 cut(s) 161, 760
AgsI TTSAA 5 cut(s) 332, 418, 506, 788, 909
AhlI ACTAGT 1 cut(s) 886
AjnI CCWGG 1 cut(s) 154
AleI CACNNNNGTG 1 cut(s) 722
AluBI AGCT 7 cut(s) 201, 219, 259, 446, 698, 873, 923
AluI AGCT 7 cut(s) 201, 219, 259, 446, 698, 873, 923
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 2 cut(s) 83, 459
AlwI GGATC 1 cut(s) 771
AlwNI CAGNNNCTG 1 cut(s) 760
Aor13HI TCCGGA 1 cut(s) 657
AoxI GGCC 1 cut(s) 700
ApeKI GCWGC 3 cut(s) 242, 443, 446
ApoI RAATTY 2 cut(s) 188, 435
AseI ATTAAT 1 cut(s) 462
AspS9I GGNCC 1 cut(s) 340
AsuHPI GGTGA 4 cut(s) 91, 540, 676, 739
AvaII GGWCC 1 cut(s) 340
BbsI GAAGAC 2 cut(s) 213, 406
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 3 cut(s) 254, 433, 455
BccI CCATC 2 cut(s) 306, 559
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 1 cut(s) 156
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 2 cut(s) 83, 459
BcuI ACTAGT 1 cut(s) 886
BfaI CTAG 1 cut(s) 887
BfmI CTRYAG 1 cut(s) 808
BglII AGATCT 1 cut(s) 8
BisI GCNGC 3 cut(s) 243, 444, 447
BlsI GCNGC 3 cut(s) 244, 445, 448
BmcAI AGTACT 1 cut(s) 122
Bme1390I CCNGG 1 cut(s) 156
Bme18I GGWCC 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 340
BmrFI CCNGG 1 cut(s) 156
BmsI GCATC 2 cut(s) 481, 489
BpiI GAAGAC 2 cut(s) 213, 406
BplI GAGNNNNNCTC 2 cut(s) 857, 889
BsaBI GATNNNNATC 1 cut(s) 550
BsaJI CCNNGG 3 cut(s) 102, 155, 222
BsaWI WCCGGW 1 cut(s) 657
BsaXI ACNNNNNCTCC 2 cut(s) 821, 851
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 760
Bse118I RCCGGY 1 cut(s) 702
Bse1I ACTGG 2 cut(s) 754, 822
Bse3DI GCAATG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 550
BseAI TCCGGA 1 cut(s) 657
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 3 cut(s) 102, 155, 222
BseGI GGATG 1 cut(s) 712
BseJI GATNNNNATC 1 cut(s) 550
BseLI CCNNNNNNNGG 2 cut(s) 161, 760
BseMI GCAATG 1 cut(s) 456
BseNI ACTGG 2 cut(s) 754, 822
BseXI GCAGC 3 cut(s) 254, 433, 455
BshFI GGCC 1 cut(s) 702
BsiHKAI GWGCWC 1 cut(s) 585
BsiSI CCGG 2 cut(s) 658, 703
BslI CCNNNNNNNGG 2 cut(s) 161, 760
BsmAI GTCTC 2 cut(s) 83, 459
BsmI GAATGC 1 cut(s) 775
BsnI GGCC 1 cut(s) 702
Bsp1286I GDGCHC 1 cut(s) 585
Bsp13I TCCGGA 1 cut(s) 657
Bsp1407I TGTACA 1 cut(s) 277
Bsp143I GATC 4 cut(s) 8, 36, 545, 763
BspACI CCGC 1 cut(s) 343
BspANI GGCC 1 cut(s) 702
BspEI TCCGGA 1 cut(s) 657
BspHI TCATGA 1 cut(s) 513
BspMAI CTGCAG 1 cut(s) 812
BspPI GGATC 1 cut(s) 771
BsrDI GCAATG 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 702
BsrGI TGTACA 1 cut(s) 277
BsrI ACTGG 2 cut(s) 754, 822
BssAI RCCGGY 1 cut(s) 702
BssECI CCNNGG 3 cut(s) 102, 155, 222
BssMI GATC 4 cut(s) 8, 36, 545, 763
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 1 cut(s) 624
Bst6I CTCTTC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 580
BstAUI TGTACA 1 cut(s) 277
BstC8I GCNNGC 3 cut(s) 576, 700, 850
BstDSI CCRYGG 1 cut(s) 102
BstENI CCTNNNNNAGG 1 cut(s) 159
BstF5I GGATG 1 cut(s) 712
BstKTI GATC 4 cut(s) 11, 39, 548, 766
BstMAI GTCTC 2 cut(s) 83, 459
BstMBI GATC 4 cut(s) 8, 36, 545, 763
BstMWI GCNNNNNNNGC 3 cut(s) 580, 790, 920
BstNI CCWGG 1 cut(s) 156
BstNSI RCATGY 1 cut(s) 132
BstSCI CCNGG 1 cut(s) 154
BstSFI CTRYAG 1 cut(s) 808
BstV1I GCAGC 3 cut(s) 254, 433, 455
BstV2I GAAGAC 2 cut(s) 213, 406
BstX2I RGATCY 2 cut(s) 8, 763
BstXI CCANNNNNNTGG 1 cut(s) 724
BstYI RGATCY 2 cut(s) 8, 763
BsuRI GGCC 1 cut(s) 702
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 1 cut(s) 712
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 2 cut(s) 227, 629
Cac8I GCNNGC 3 cut(s) 576, 700, 850
CaiI CAGNNNCTG 1 cut(s) 760
CciI TCATGA 1 cut(s) 513
Cfr10I RCCGGY 1 cut(s) 702
Cfr13I GGNCC 1 cut(s) 340
Csp6I GTAC 2 cut(s) 121, 278
CspCI CAANNNNNGTGG 2 cut(s) 870, 905
CviAII CATG 2 cut(s) 129, 514
CviQI GTAC 2 cut(s) 121, 278
DpnI GATC 4 cut(s) 10, 38, 547, 765
DpnII GATC 4 cut(s) 8, 36, 545, 763
EaeI YGGCCR 1 cut(s) 700
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 340
EcoNI CCTNNNNNAGG 1 cut(s) 159
EcoRI GAATTC 1 cut(s) 435
EcoRII CCWGG 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 2 cut(s) 132, 517
FalI AAGNNNNNCTT 4 cut(s) 185, 217, 653, 685
FatI CATG 2 cut(s) 128, 513
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 3 cut(s) 243, 444, 447
FokI GGATG 1 cut(s) 699
Fsp4HI GCNGC 3 cut(s) 243, 444, 447
FspBI CTAG 1 cut(s) 887
GluI GCNGC 3 cut(s) 243, 444, 447
HaeIII GGCC 1 cut(s) 702
HapII CCGG 2 cut(s) 658, 703
Hin1II CATG 2 cut(s) 132, 517
HincII GTYRAC 2 cut(s) 645, 815
HindII GTYRAC 2 cut(s) 645, 815
HinfI GANTC 4 cut(s) 81, 324, 638, 750
HpaII CCGG 2 cut(s) 658, 703
HphI GGTGA 4 cut(s) 91, 540, 676, 739
Hpy166II GTNNAC 4 cut(s) 99, 645, 667, 815
Hpy188I TCNGA 5 cut(s) 57, 459, 587, 768, 802
Hpy188III TCNNGA 5 cut(s) 467, 506, 514, 658, 827
Hpy8I GTNNAC 4 cut(s) 99, 645, 667, 815
HpyAV CCTTC 3 cut(s) 20, 71, 655
HpyCH4III ACNGT 1 cut(s) 624
HpyCH4V TGCA 8 cut(s) 16, 128, 449, 494, 675, 682, 773, 810
HpyF10VI GCNNNNNNNGC 3 cut(s) 580, 790, 920
Hsp92II CATG 2 cut(s) 132, 517
Kpn2I TCCGGA 1 cut(s) 657
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 4 cut(s) 8, 36, 545, 763
LmnI GCTCC 1 cut(s) 224
Lsp1109I GCAGC 3 cut(s) 254, 433, 455
LweI GCATC 2 cut(s) 481, 489
MaeI CTAG 1 cut(s) 887
MalI GATC 4 cut(s) 10, 38, 547, 765
MboI GATC 4 cut(s) 8, 36, 545, 763
MboII GAAGA 7 cut(s) 56, 79, 119, 218, 406, 609, 897
MfeI CAATTG 1 cut(s) 390
MflI RGATCY 2 cut(s) 8, 763
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 7 cut(s) 51, 188, 292, 390, 435, 531, 611
MnlI CCTC 5 cut(s) 40, 163, 244, 397, 859
Mph1103I ATGCAT 1 cut(s) 130
MroI TCCGGA 1 cut(s) 657
MseI TTAA 7 cut(s) 32, 192, 266, 462, 734, 854, 934
MslI CAYNNNNRTG 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 2 cut(s) 658, 703
MspR9I CCNGG 1 cut(s) 156
MunI CAATTG 1 cut(s) 390
Mva1269I GAATGC 1 cut(s) 775
MvaI CCWGG 1 cut(s) 156
MwoI GCNNNNNNNGC 3 cut(s) 580, 790, 920
NdeII GATC 4 cut(s) 8, 36, 545, 763
NlaIII CATG 2 cut(s) 132, 517
NsiI ATGCAT 1 cut(s) 130
NspI RCATGY 1 cut(s) 132
OliI CACNNNNGTG 1 cut(s) 722
PagI TCATGA 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PctI GAATGC 1 cut(s) 775
PfeI GAWTC 4 cut(s) 81, 324, 638, 750
PflMI CCANNNNNTGG 1 cut(s) 760
PkrI GCNGC 3 cut(s) 244, 445, 448
PshBI ATTAAT 1 cut(s) 462
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspPI GGNCC 1 cut(s) 340
PstI CTGCAG 1 cut(s) 812
PstNI CAGNNNCTG 1 cut(s) 760
PsuI RGATCY 2 cut(s) 8, 763
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 2 cut(s) 122, 279
RsaNI GTAC 2 cut(s) 121, 278
RseI CAYNNNNRTG 1 cut(s) 722
SaqAI TTAA 7 cut(s) 32, 192, 266, 462, 734, 854, 934
SatI GCNGC 3 cut(s) 243, 444, 447
Sau3AI GATC 4 cut(s) 8, 36, 545, 763
Sau96I GGNCC 1 cut(s) 340
ScaI AGTACT 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 2 cut(s) 481, 489
SfcI CTRYAG 1 cut(s) 808
SinI GGWCC 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 722
SpeI ACTAGT 1 cut(s) 886
Sse9I AATT 7 cut(s) 51, 188, 292, 390, 435, 531, 611
SsiI CCGC 1 cut(s) 343
SspMI CTAG 1 cut(s) 887
StyD4I CCNGG 1 cut(s) 154
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 624
TasI AATT 7 cut(s) 51, 188, 292, 390, 435, 531, 611
TatI WGTACW 2 cut(s) 120, 277
TfiI GAWTC 4 cut(s) 81, 324, 638, 750
Tru1I TTAA 7 cut(s) 32, 192, 266, 462, 734, 854, 934
Tru9I TTAA 7 cut(s) 32, 192, 266, 462, 734, 854, 934
TscAI CASTG 2 cut(s) 234, 629
TseI GCWGC 3 cut(s) 242, 443, 446
TspDTI ATGAA 4 cut(s) 17, 39, 131, 920
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 2 cut(s) 234, 629
Van91I CCANNNNNTGG 1 cut(s) 760
VpaK11BI GGWCC 1 cut(s) 340
VspI ATTAAT 1 cut(s) 462
XagI CCTNNNNNAGG 1 cut(s) 159
XapI RAATTY 2 cut(s) 188, 435
XceI RCATGY 1 cut(s) 132
XspI CTAG 1 cut(s) 887
ZrmI AGTACT 1 cut(s) 122
Zsp2I ATGCAT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.