Rh5AG200300

Cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018400
Physical Location & Seq
Reverse (-)
23571148 .. 23578062
6915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG200300.1

Sequence Viewer

Length: 816 bp
ATGCGCCGTCTTCTTACTCCAGCAATAGCGCCAATAACGGCGGCAGTGGTCTTCCGACTTCCGGCGTTTTCCATGACTCGACCTACTAGATCTCCGTCTCCGGCGGCACTGCCCTCGTTGGCAGCGAAAGAAAATGAGTTTCTGTTAAGGGAACTTGTACAGATGTGGTCAAATTACAAGATAATGGCAAGATGGCTATGTAGATTCTTTGAATACTTGGACCGCTATTATATCCCCCGTCATTGTCTTGTTTCACTTACTGACACCTCAATTGATTGCTTTTGTAATCTGGTCTTCAATGGGTTGGCTAACAGAATTCAGGCAGCTGCAATGTCTCTGATTAATCAAGAGCGTTATGGACAACTGATTGATGCAAAGATGCTTCACGATGTCATGAGTATCTTTATGGAAATTGGTCAAAATAGATCGCCATCATACTATGAAATCATTGAGCAGGCTGTGCTCGGAGAAACTGCCACTTTCTATTCCCAATTATCTTCACAGTGGCTTTTGTGCGATTCGTTTACTGATTATATCCGGAAGGTGAACTGGTGCATTTTGCAGGAGAAAGAAAGAGCTGGCCGGTATCTACATCCCACATCTGTGGCGAAGTTAATACAGGTTGTGCGATTCCAGTTGCTGGATCTGAATGCAAATAAGTTGCTTGAAAAGCAGAAGTCTGAAAACTGCAGTCAACTGGATTATCAGGAGATATTGGCAAAGTGTGCCTGCTTAAATCTTGGAGAGGACAGCTCTACATCAACACTAGTGGAAAATCTCTGTTCTTCAATGGCACAATCAGCTCATATTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04114 ko04120 ko04141 ko04310 ko04340 ko04341 ko04350 ko04710 ko05168 ko05200 map04110 map04111 map04114 map04120 map04141 map04310 map04340 map04341 map04350 map04710 map05168 map05200
Pfam Domains
Protein Families
Cullin

Protein Analysis

271

Amino Acids

31.03

Weight (kDa)

7.52

Isoelectric Point (pI)

58.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 42 - 231 7.1e-37 Cullin alpha solenoid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018400)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g17070 FvH4_3g17070 FvH4_3g17070
rosa_chinensis RchiOBHm_Chr5g0028421
rosa_laevigata RLG00000033093
rosa_multiflora Rmu_sc0006771.1_g000005
rosa_roxburghii Rroxscaffold_1G00051260
rosa_rugosa Rorug05G0107500
rosa_samantha Rh5AG200300 Rh5BG198700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 640
AccIII TCCGGA 1 cut(s) 537
AciI CCGC 3 cut(s) 41, 104, 223
AclWI GGATC 1 cut(s) 651
AcoI YGGCCR 1 cut(s) 580
AcsI RAATTY 1 cut(s) 315
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 2 cut(s) 61, 640
AgsI TTSAA 4 cut(s) 212, 298, 668, 789
AhlI ACTAGT 1 cut(s) 766
AleI CACNNNNGTG 1 cut(s) 602
AluBI AGCT 4 cut(s) 326, 578, 753, 803
AluI AGCT 4 cut(s) 326, 578, 753, 803
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 2 cut(s) 102, 339
AlwI GGATC 1 cut(s) 651
AlwNI CAGNNNCTG 1 cut(s) 640
Aor13HI TCCGGA 1 cut(s) 537
AoxI GGCC 1 cut(s) 580
ApeKI GCWGC 3 cut(s) 122, 323, 326
ApoI RAATTY 1 cut(s) 315
AseI ATTAAT 1 cut(s) 342
AspLEI GCGC 2 cut(s) 6, 31
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 1 cut(s) 556
AvaII GGWCC 1 cut(s) 220
BbsI GAAGAC 3 cut(s) 2, 43, 286
Bbv12I GWGCWC 1 cut(s) 465
BbvI GCAGC 3 cut(s) 134, 313, 335
BccI CCATC 2 cut(s) 186, 439
BceAI ACGGC 1 cut(s) 54
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BcoDI GTCTC 2 cut(s) 102, 339
BcuI ACTAGT 1 cut(s) 766
BfaI CTAG 2 cut(s) 87, 767
BfmI CTRYAG 1 cut(s) 688
BfoI RGCGCY 1 cut(s) 32
BglII AGATCT 1 cut(s) 89
BisI GCNGC 5 cut(s) 42, 105, 123, 324, 327
BlsI GCNGC 5 cut(s) 43, 106, 124, 325, 328
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmsI GCATC 2 cut(s) 361, 369
BpiI GAAGAC 3 cut(s) 2, 43, 286
BplI GAGNNNNNCTC 2 cut(s) 737, 769
BsaBI GATNNNNATC 1 cut(s) 430
BsaWI WCCGGW 1 cut(s) 537
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 640
Bse118I RCCGGY 1 cut(s) 582
Bse1I ACTGG 3 cut(s) 554, 634, 702
Bse3DI GCAATG 1 cut(s) 336
Bse8I GATNNNNATC 1 cut(s) 430
BseAI TCCGGA 1 cut(s) 537
BseGI GGATG 1 cut(s) 592
BseJI GATNNNNATC 1 cut(s) 430
BseLI CCNNNNNNNGG 2 cut(s) 61, 640
BseMI GCAATG 1 cut(s) 336
BseNI ACTGG 3 cut(s) 554, 634, 702
BseXI GCAGC 3 cut(s) 134, 313, 335
BshFI GGCC 1 cut(s) 582
BsiHKAI GWGCWC 1 cut(s) 465
BsiSI CCGG 4 cut(s) 62, 101, 538, 583
BslI CCNNNNNNNGG 2 cut(s) 61, 640
BsmAI GTCTC 2 cut(s) 102, 339
BsmBI CGTCTC 1 cut(s) 102
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 1 cut(s) 582
Bsp1286I GDGCHC 1 cut(s) 465
Bsp13I TCCGGA 1 cut(s) 537
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 3 cut(s) 89, 425, 643
BspACI CCGC 3 cut(s) 41, 104, 223
BspANI GGCC 1 cut(s) 582
BspEI TCCGGA 1 cut(s) 537
BspHI TCATGA 1 cut(s) 393
BspMAI CTGCAG 1 cut(s) 692
BspPI GGATC 1 cut(s) 651
BsrDI GCAATG 1 cut(s) 336
BsrFI RCCGGY 1 cut(s) 582
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 3 cut(s) 554, 634, 702
BssAI RCCGGY 1 cut(s) 582
BssMI GATC 3 cut(s) 89, 425, 643
Bst4CI ACNGT 1 cut(s) 504
BstAPI GCANNNNNTGC 2 cut(s) 460, 725
BstAUI TGTACA 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 456, 580, 730
BstF5I GGATG 1 cut(s) 592
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 6, 31
BstKTI GATC 3 cut(s) 92, 428, 646
BstMAI GTCTC 2 cut(s) 102, 339
BstMBI GATC 3 cut(s) 89, 425, 643
BstMWI GCNNNNNNNGC 4 cut(s) 460, 670, 725, 800
BstSFI CTRYAG 1 cut(s) 688
BstV1I GCAGC 3 cut(s) 134, 313, 335
BstV2I GAAGAC 3 cut(s) 2, 43, 286
BstX2I RGATCY 2 cut(s) 89, 643
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 2 cut(s) 89, 643
BsuRI GGCC 1 cut(s) 582
BtsCI GGATG 1 cut(s) 592
BtsI GCAGTG 2 cut(s) 51, 107
BtsIMutI CAGTG 3 cut(s) 51, 107, 509
Cac8I GCNNGC 3 cut(s) 456, 580, 730
CaiI CAGNNNCTG 1 cut(s) 640
CciI TCATGA 1 cut(s) 393
CfoI GCGC 2 cut(s) 6, 31
Cfr10I RCCGGY 1 cut(s) 582
Cfr13I GGNCC 1 cut(s) 220
Csp6I GTAC 1 cut(s) 158
CspCI CAANNNNNGTGG 2 cut(s) 750, 785
CviAII CATG 2 cut(s) 73, 394
CviJI RGCY 9 cut(s) 196, 308, 326, 458, 508, 578, 582, 753, 803
CviKI_1 RGCY 9 cut(s) 196, 308, 326, 458, 508, 578, 582, 753, 803
CviQI GTAC 1 cut(s) 158
DpnI GATC 3 cut(s) 91, 427, 645
DpnII GATC 3 cut(s) 89, 425, 643
EaeI YGGCCR 1 cut(s) 580
Eco47I GGWCC 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 102
FaeI CATG 2 cut(s) 76, 397
FatI CATG 2 cut(s) 72, 393
Fnu4HI GCNGC 5 cut(s) 42, 105, 123, 324, 327
FokI GGATG 1 cut(s) 579
Fsp4HI GCNGC 5 cut(s) 42, 105, 123, 324, 327
FspBI CTAG 2 cut(s) 87, 767
GlaI GCGC 2 cut(s) 5, 30
GluI GCNGC 5 cut(s) 42, 105, 123, 324, 327
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 1 cut(s) 582
HapII CCGG 4 cut(s) 62, 101, 538, 583
HhaI GCGC 2 cut(s) 6, 31
Hin1II CATG 2 cut(s) 76, 397
Hin6I GCGC 2 cut(s) 4, 29
HinP1I GCGC 2 cut(s) 4, 29
HincII GTYRAC 1 cut(s) 695
HindII GTYRAC 1 cut(s) 695
HinfI GANTC 4 cut(s) 76, 204, 518, 630
HpaII CCGG 4 cut(s) 62, 101, 538, 583
HphI GGTGA 1 cut(s) 556
Hpy166II GTNNAC 3 cut(s) 525, 547, 695
Hpy188I TCNGA 5 cut(s) 56, 339, 467, 648, 682
Hpy188III TCNNGA 5 cut(s) 347, 386, 394, 538, 707
Hpy8I GTNNAC 3 cut(s) 525, 547, 695
HpyAV CCTTC 1 cut(s) 535
HpyCH4III ACNGT 1 cut(s) 504
HpyCH4V TGCA 6 cut(s) 329, 374, 555, 562, 653, 690
HpyF10VI GCNNNNNNNGC 4 cut(s) 460, 670, 725, 800
Hsp92II CATG 2 cut(s) 76, 397
HspAI GCGC 2 cut(s) 4, 29
Kpn2I TCCGGA 1 cut(s) 537
Kzo9I GATC 3 cut(s) 89, 425, 643
Lsp1109I GCAGC 3 cut(s) 134, 313, 335
LweI GCATC 2 cut(s) 361, 369
MaeI CTAG 2 cut(s) 87, 767
MalI GATC 3 cut(s) 91, 427, 645
MboI GATC 3 cut(s) 89, 425, 643
MboII GAAGA 4 cut(s) 43, 286, 489, 777
MfeI CAATTG 1 cut(s) 270
MflI RGATCY 2 cut(s) 89, 643
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 5 cut(s) 172, 270, 315, 411, 491
MlyI GAGTC 1 cut(s) 70
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 3 cut(s) 124, 277, 739
MroI TCCGGA 1 cut(s) 537
MseI TTAA 5 cut(s) 146, 342, 614, 734, 814
MslI CAYNNNNRTG 1 cut(s) 602
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 4 cut(s) 62, 101, 538, 583
MunI CAATTG 1 cut(s) 270
Mva1269I GAATGC 1 cut(s) 655
MwoI GCNNNNNNNGC 4 cut(s) 460, 670, 725, 800
NdeII GATC 3 cut(s) 89, 425, 643
NlaIII CATG 2 cut(s) 76, 397
OliI CACNNNNGTG 1 cut(s) 602
PagI TCATGA 1 cut(s) 393
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PctI GAATGC 1 cut(s) 655
PfeI GAWTC 3 cut(s) 204, 518, 630
PflMI CCANNNNNTGG 1 cut(s) 640
PkrI GCNGC 5 cut(s) 43, 106, 124, 325, 328
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PshBI ATTAAT 1 cut(s) 342
PspPI GGNCC 1 cut(s) 220
PstI CTGCAG 1 cut(s) 692
PstNI CAGNNNCTG 1 cut(s) 640
PsuI RGATCY 2 cut(s) 89, 643
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
RseI CAYNNNNRTG 1 cut(s) 602
SaqAI TTAA 5 cut(s) 146, 342, 614, 734, 814
SatI GCNGC 5 cut(s) 42, 105, 123, 324, 327
Sau3AI GATC 3 cut(s) 89, 425, 643
Sau96I GGNCC 1 cut(s) 220
SchI GAGTC 1 cut(s) 70
SduI GDGCHC 1 cut(s) 465
SetI ASST 8 cut(s) 85, 269, 328, 546, 580, 624, 755, 805
SfaNI GCATC 2 cut(s) 361, 369
SfcI CTRYAG 1 cut(s) 688
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 602
SpeI ACTAGT 1 cut(s) 766
Sse9I AATT 5 cut(s) 172, 270, 315, 411, 491
SsiI CCGC 3 cut(s) 41, 104, 223
SspMI CTAG 2 cut(s) 87, 767
TaaI ACNGT 1 cut(s) 504
TaqI TCGA 1 cut(s) 79
TasI AATT 5 cut(s) 172, 270, 315, 411, 491
TatI WGTACW 1 cut(s) 157
TauI GCSGC 2 cut(s) 44, 107
TfiI GAWTC 3 cut(s) 204, 518, 630
Tru1I TTAA 5 cut(s) 146, 342, 614, 734, 814
Tru9I TTAA 5 cut(s) 146, 342, 614, 734, 814
TscAI CASTG 3 cut(s) 51, 114, 509
TseI GCWGC 3 cut(s) 122, 323, 326
TspDTI ATGAA 2 cut(s) 456, 800
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 3 cut(s) 51, 114, 509
Van91I CCANNNNNTGG 1 cut(s) 640
VpaK11BI GGWCC 1 cut(s) 220
VspI ATTAAT 1 cut(s) 342
XapI RAATTY 1 cut(s) 315
XspI CTAG 2 cut(s) 87, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.