RLG00000033093

Cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018400
Physical Location & Seq
Reverse (-)
23227761 .. 23230880
3120 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033093

Sequence Viewer

Length: 936 bp
ATGAATAGATCTATGCAAGTTGATGAAGGGTTAATGATTATAGAAGAGGCAATTCAGAAAGCAAAGAAGATTGTGGAAGGATTCCCTGAGACAAAGTTCACTACAGAAGAATATATGAAGTACTATGCATGTGTTTATATTATGTGCGATAAGCCTGGGGCTGGAAACGAGGTGTTGCTATATGAAAAATTTAAGAAGAGCTTGGAAGACATCATCAGCTCCTCGGCACTGCCCTCGTTGGCAGCGAAAGAAAATGAGCTTCTGTTAAGGGAACTTGTACAGATGTGGTCAAATTACAAGATAATGGCGAGATGGCTATGTAGGTTCTTTGAATACATGGACCGCTATTATATCCCCCGTCATTGTCTTCTTTCACTTACTGACACCTCAATTGATTGCTTTTGTAATCTGGTCTTCAATGGGTTGTCTAACAGAATTCAGGCAGCTGCAATGTCTCTGATTAATCAAGAGCGTTATGGACAACTGATTGATGCAAAGCTGCTTCAAGATGTCATGAGTATCTTTATGGAAATTGGTCAAAATAGATCGCCATCATACTATGAAATCATTGAGCAGGCTGTGCTTGGAGAAACTGCCACTTTCTATTCCCAATTATCTTCACAGTGGCTTTTGTGCGATTCGTTGGCCAATTATATCCAGAAGGTGAACTCGTGCATTTTGCAGGAGAAAGAAAGAGCTGGCCGCTATCTACATCCCACATCTGTGGCGAAGATAATACAGGTTGTGCGATTCCAATTGCTGGATCTGAAAGCAAATAAGTTGCTTGAAAAGCAGAAGTTTGAAAACTGCAGTCAACTGGATTATCAGGAGATATTGTCAAAGTGTGCCTGCTTAAATCTTGGAGAGGACAGCTCCACATCAACACTAGTGGAAAATCTCTGTTCTTCAATGGCACAATCAGCTCATATTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04114 ko04120 ko04141 ko04310 ko04340 ko04341 ko04350 ko04710 ko05168 ko05200 map04110 map04111 map04114 map04120 map04141 map04310 map04340 map04341 map04350 map04710 map05168 map05200
Pfam Domains
Protein Families
Cullin

Protein Analysis

312

Amino Acids

35.77

Weight (kDa)

5.44

Isoelectric Point (pI)

53.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 30 - 279 1.3e-45 Cullin alpha solenoid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018400)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g17070 FvH4_3g17070 FvH4_3g17070
rosa_chinensis RchiOBHm_Chr5g0028421
rosa_laevigata RLG00000033093
rosa_multiflora Rmu_sc0006771.1_g000005
rosa_roxburghii Rroxscaffold_1G00051260
rosa_rugosa Rorug05G0107500
rosa_samantha Rh5AG200300 Rh5BG198700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 760
AciI CCGC 2 cut(s) 343, 703
AclWI GGATC 1 cut(s) 771
AcoI YGGCCR 2 cut(s) 645, 700
AcsI RAATTY 2 cut(s) 188, 435
AfaI GTAC 2 cut(s) 122, 279
AfiI CCNNNNNNNGG 2 cut(s) 161, 760
AgsI TTSAA 6 cut(s) 332, 418, 506, 788, 803, 909
AhlI ACTAGT 1 cut(s) 886
AjnI CCWGG 1 cut(s) 154
AleI CACNNNNGTG 1 cut(s) 722
AluBI AGCT 8 cut(s) 201, 219, 259, 446, 499, 698, 873, 923
AluI AGCT 8 cut(s) 201, 219, 259, 446, 499, 698, 873, 923
Alw26I GTCTC 2 cut(s) 83, 459
AlwI GGATC 1 cut(s) 771
AoxI GGCC 2 cut(s) 645, 700
ApeKI GCWGC 4 cut(s) 242, 443, 446, 499
ApoI RAATTY 2 cut(s) 188, 435
AseI ATTAAT 1 cut(s) 462
AspS9I GGNCC 1 cut(s) 340
AsuHPI GGTGA 1 cut(s) 676
AvaII GGWCC 1 cut(s) 340
BalI TGGCCA 1 cut(s) 647
BauI CACGAG 1 cut(s) 670
BbsI GAAGAC 3 cut(s) 213, 359, 406
BbvI GCAGC 4 cut(s) 254, 433, 455, 486
BccI CCATC 2 cut(s) 306, 559
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 1 cut(s) 156
BcoDI GTCTC 2 cut(s) 83, 459
BcuI ACTAGT 1 cut(s) 886
BfaI CTAG 1 cut(s) 887
BfmI CTRYAG 2 cut(s) 102, 808
BglII AGATCT 1 cut(s) 8
BisI GCNGC 5 cut(s) 243, 444, 447, 500, 703
BlsI GCNGC 5 cut(s) 244, 445, 448, 501, 704
BmcAI AGTACT 1 cut(s) 122
Bme1390I CCNGG 1 cut(s) 156
Bme18I GGWCC 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 340
BmrFI CCNGG 1 cut(s) 156
BmsI GCATC 1 cut(s) 481
BpiI GAAGAC 3 cut(s) 213, 359, 406
BplI GAGNNNNNCTC 2 cut(s) 857, 889
BsaBI GATNNNNATC 1 cut(s) 550
BsaJI CCNNGG 2 cut(s) 155, 222
BsaXI ACNNNNNCTCC 2 cut(s) 821, 851
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 760
Bse1I ACTGG 1 cut(s) 822
Bse3DI GCAATG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 550
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 155, 222
BseGI GGATG 1 cut(s) 712
BseJI GATNNNNATC 1 cut(s) 550
BseLI CCNNNNNNNGG 2 cut(s) 161, 760
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 1 cut(s) 822
BseRI GAGGAG 1 cut(s) 211
BseXI GCAGC 4 cut(s) 254, 433, 455, 486
BshFI GGCC 2 cut(s) 647, 702
BslI CCNNNNNNNGG 2 cut(s) 161, 760
BsmAI GTCTC 2 cut(s) 83, 459
BsnI GGCC 2 cut(s) 647, 702
Bsp1407I TGTACA 1 cut(s) 277
Bsp143I GATC 3 cut(s) 8, 545, 763
BspACI CCGC 2 cut(s) 343, 703
BspANI GGCC 2 cut(s) 647, 702
BspCNI CTCAG 1 cut(s) 79
BspHI TCATGA 1 cut(s) 513
BspMAI CTGCAG 1 cut(s) 812
BspPI GGATC 1 cut(s) 771
BspQI GCTCTTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 456
BsrGI TGTACA 1 cut(s) 277
BsrI ACTGG 1 cut(s) 822
BssECI CCNNGG 2 cut(s) 155, 222
BssMI GATC 3 cut(s) 8, 545, 763
BssSI CACGAG 1 cut(s) 670
Bst2BI CACGAG 1 cut(s) 670
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 1 cut(s) 624
Bst6I CTCTTC 2 cut(s) 39, 191
BstAPI GCANNNNNTGC 1 cut(s) 580
BstAUI TGTACA 1 cut(s) 277
BstC8I GCNNGC 3 cut(s) 576, 700, 850
BstDEI CTNAG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 712
BstKTI GATC 3 cut(s) 11, 548, 766
BstMAI GTCTC 2 cut(s) 83, 459
BstMBI GATC 3 cut(s) 8, 545, 763
BstMWI GCNNNNNNNGC 3 cut(s) 580, 790, 920
BstNI CCWGG 1 cut(s) 156
BstNSI RCATGY 1 cut(s) 132
BstSCI CCNGG 1 cut(s) 154
BstSFI CTRYAG 2 cut(s) 102, 808
BstV1I GCAGC 4 cut(s) 254, 433, 455, 486
BstV2I GAAGAC 3 cut(s) 213, 359, 406
BstX2I RGATCY 2 cut(s) 8, 763
BstXI CCANNNNNNTGG 1 cut(s) 724
BstYI RGATCY 2 cut(s) 8, 763
BsuRI GGCC 2 cut(s) 647, 702
BtsCI GGATG 1 cut(s) 712
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 2 cut(s) 227, 629
Cac8I GCNNGC 3 cut(s) 576, 700, 850
CciI TCATGA 1 cut(s) 513
Cfr13I GGNCC 1 cut(s) 340
Csp6I GTAC 2 cut(s) 121, 278
CspCI CAANNNNNGTGG 2 cut(s) 870, 905
CviAII CATG 3 cut(s) 129, 337, 514
CviQI GTAC 2 cut(s) 121, 278
DdeI CTNAG 1 cut(s) 87
DpnI GATC 3 cut(s) 10, 547, 765
DpnII GATC 3 cut(s) 8, 545, 763
EaeI YGGCCR 2 cut(s) 645, 700
Eam1104I CTCTTC 2 cut(s) 39, 191
EarI CTCTTC 2 cut(s) 39, 191
Eco47I GGWCC 1 cut(s) 340
EcoRI GAATTC 1 cut(s) 435
EcoRII CCWGG 1 cut(s) 154
EcoT22I ATGCAT 1 cut(s) 130
FaeI CATG 3 cut(s) 132, 340, 517
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FatI CATG 3 cut(s) 128, 336, 513
Fnu4HI GCNGC 5 cut(s) 243, 444, 447, 500, 703
FokI GGATG 1 cut(s) 699
Fsp4HI GCNGC 5 cut(s) 243, 444, 447, 500, 703
FspBI CTAG 1 cut(s) 887
GluI GCNGC 5 cut(s) 243, 444, 447, 500, 703
HaeIII GGCC 2 cut(s) 647, 702
Hin1II CATG 3 cut(s) 132, 340, 517
HincII GTYRAC 1 cut(s) 815
HindII GTYRAC 1 cut(s) 815
HinfI GANTC 3 cut(s) 81, 638, 750
HphI GGTGA 1 cut(s) 676
Hpy166II GTNNAC 3 cut(s) 99, 667, 815
Hpy188I TCNGA 3 cut(s) 57, 459, 768
Hpy188III TCNNGA 5 cut(s) 467, 506, 514, 658, 827
Hpy8I GTNNAC 3 cut(s) 99, 667, 815
HpyAV CCTTC 3 cut(s) 20, 71, 655
HpyCH4III ACNGT 1 cut(s) 624
HpyCH4V TGCA 7 cut(s) 16, 128, 449, 494, 675, 682, 810
HpyF10VI GCNNNNNNNGC 3 cut(s) 580, 790, 920
HpyF3I CTNAG 1 cut(s) 87
Hsp92II CATG 3 cut(s) 132, 340, 517
Kzo9I GATC 3 cut(s) 8, 545, 763
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 2 cut(s) 224, 878
Lsp1109I GCAGC 4 cut(s) 254, 433, 455, 486
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 887
MalI GATC 3 cut(s) 10, 547, 765
MboI GATC 3 cut(s) 8, 545, 763
MfeI CAATTG 2 cut(s) 390, 755
MflI RGATCY 2 cut(s) 8, 763
MlsI TGGCCA 1 cut(s) 647
MluCI AATT 9 cut(s) 51, 188, 292, 390, 435, 531, 611, 649, 755
MluNI TGGCCA 1 cut(s) 647
MnlI CCTC 6 cut(s) 40, 163, 232, 244, 397, 859
Mox20I TGGCCA 1 cut(s) 647
Mph1103I ATGCAT 1 cut(s) 130
MscI TGGCCA 1 cut(s) 647
MseI TTAA 6 cut(s) 32, 192, 266, 462, 854, 934
MslI CAYNNNNRTG 1 cut(s) 722
Msp20I TGGCCA 1 cut(s) 647
MspA1I CMGCKG 1 cut(s) 446
MspR9I CCNGG 1 cut(s) 156
MunI CAATTG 2 cut(s) 390, 755
MvaI CCWGG 1 cut(s) 156
MwoI GCNNNNNNNGC 3 cut(s) 580, 790, 920
NdeII GATC 3 cut(s) 8, 545, 763
NlaIII CATG 3 cut(s) 132, 340, 517
NmeAIII GCCGAG 1 cut(s) 203
NsiI ATGCAT 1 cut(s) 130
NspI RCATGY 1 cut(s) 132
OliI CACNNNNGTG 1 cut(s) 722
PagI TCATGA 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PfeI GAWTC 3 cut(s) 81, 638, 750
PflMI CCANNNNNTGG 1 cut(s) 760
PkrI GCNGC 5 cut(s) 244, 445, 448, 501, 704
PshBI ATTAAT 1 cut(s) 462
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspPI GGNCC 1 cut(s) 340
PstI CTGCAG 1 cut(s) 812
PsuI RGATCY 2 cut(s) 8, 763
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 2 cut(s) 122, 279
RsaNI GTAC 2 cut(s) 121, 278
RseI CAYNNNNRTG 1 cut(s) 722
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 6 cut(s) 32, 192, 266, 462, 854, 934
SatI GCNGC 5 cut(s) 243, 444, 447, 500, 703
Sau3AI GATC 3 cut(s) 8, 545, 763
Sau96I GGNCC 1 cut(s) 340
ScaI AGTACT 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 156
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 2 cut(s) 102, 808
SinI GGWCC 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 722
SpeI ACTAGT 1 cut(s) 886
Sse9I AATT 9 cut(s) 51, 188, 292, 390, 435, 531, 611, 649, 755
SsiI CCGC 2 cut(s) 343, 703
SspMI CTAG 1 cut(s) 887
StyD4I CCNGG 1 cut(s) 154
TaaI ACNGT 1 cut(s) 624
TasI AATT 9 cut(s) 51, 188, 292, 390, 435, 531, 611, 649, 755
TatI WGTACW 2 cut(s) 120, 277
TauI GCSGC 1 cut(s) 705
TfiI GAWTC 3 cut(s) 81, 638, 750
Tru1I TTAA 6 cut(s) 32, 192, 266, 462, 854, 934
Tru9I TTAA 6 cut(s) 32, 192, 266, 462, 854, 934
TscAI CASTG 2 cut(s) 234, 629
TseI GCWGC 4 cut(s) 242, 443, 446, 499
TspDTI ATGAA 6 cut(s) 17, 39, 131, 198, 576, 920
TspRI CASTG 2 cut(s) 234, 629
Van91I CCANNNNNTGG 1 cut(s) 760
VpaK11BI GGWCC 1 cut(s) 340
VspI ATTAAT 1 cut(s) 462
XapI RAATTY 2 cut(s) 188, 435
XceI RCATGY 1 cut(s) 132
XspI CTAG 1 cut(s) 887
ZrmI AGTACT 1 cut(s) 122
Zsp2I ATGCAT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.