RchiOBHm_Chr5g0030791

14 kDa proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018613
Physical Location & Seq
Forward (+)
24456683 .. 24457180
498 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 375 bp
ATGGCTTCTTCTACCAATCTCTCTGCAACCCTTCTCGTCTTCTCAGTCCTTCTCTACCCACCACCAATACTGGCACAAACACCATTAACCCCGGTACCAACACCAGCACCAGCTATGGAAACTTGCCCGAGAGACTCGCTGAAGTTAGGGGTTTGTGCAGGCCTTCTGGGACTCGTGAACGTTAAAATTGGATCGTCAGCAACCAGTCCCTGCTGTGCACTGCTTGAAGGCTTGGCTGATTTGAAGGCTGCTCTTTGTCTTTGCACCGCGCTTAAGGCCAATGTGCTCGGTATTAACTTGGACGTGCCAATTGCTTTGAATTTGATTGTTAGTGCTTGCCAGAAAACTGTCCCTCCTGGTTTTAAATGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

124

Amino Acids

12.63

Weight (kDa)

7.48

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 30 - 123 1.1e-07 Probable lipid transfer
Hydrophob_seed PF14547 41 - 123 4.4e-27 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 42 - 123 1.5e-13 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018613)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0030791
rosa_laevigata RLG00000033253
rosa_multiflora Rmu_co8328467.1_g000001
rosa_roxburghii Rroxscaffold_1G00049500
rosa_rugosa Rorug05G0120800
rosa_samantha Rh5AG213300 Rh5BG212100 Rh5CG235400 Rh5DG216900
rosa_wichuraiana Rw5G019510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 94
AccB1I GGYRCC 1 cut(s) 94
AccII CGCG 1 cut(s) 269
AciI CCGC 1 cut(s) 267
AclI AACGTT 1 cut(s) 180
AclWI GGATC 1 cut(s) 199
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 1 cut(s) 96
AflII CTTAAG 1 cut(s) 272
AgsI TTSAA 3 cut(s) 227, 244, 319
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 355
AluBI AGCT 1 cut(s) 113
AluI AGCT 1 cut(s) 113
Alw21I GWGCWC 2 cut(s) 220, 288
Alw26I GTCTC 1 cut(s) 126
Alw44I GTGCAC 1 cut(s) 216
AlwI GGATC 1 cut(s) 199
AlwNI CAGNNNCTG 1 cut(s) 210
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 2 cut(s) 160, 276
ApaLI GTGCAC 1 cut(s) 216
ApeKI GCWGC 1 cut(s) 248
ApoI RAATTY 1 cut(s) 319
Asp718I GGTACC 1 cut(s) 94
AspLEI GCGC 1 cut(s) 271
AsuC2I CCSGG 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 220
BaeI ACNNNNGTAYC 2 cut(s) 78, 111
BanI GGYRCC 1 cut(s) 94
BauI CACGAG 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 2 cut(s) 220, 288
BbvI GCAGC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 357
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 126
BfrI CTTAAG 1 cut(s) 272
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
Bme1390I CCNGG 2 cut(s) 92, 357
BmeT110I CYCGRG 1 cut(s) 127
BmgBI CACGTC 1 cut(s) 304
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 2 cut(s) 92, 357
BpiI GAAGAC 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 90
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bse1I ACTGG 2 cut(s) 75, 204
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 90
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 2 cut(s) 75, 204
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 1 cut(s) 235
BsgI GTGCAG 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 2 cut(s) 162, 278
BshNI GGYRCC 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 220, 288
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 3 cut(s) 183, 192, 335
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 3 cut(s) 183, 192, 335
BsnI GGCC 2 cut(s) 162, 278
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 220, 288
Bsp143I GATC 1 cut(s) 191
BspACI CCGC 1 cut(s) 267
BspANI GGCC 2 cut(s) 162, 278
BspCNI CTCAG 1 cut(s) 56
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 1 cut(s) 96
BspPI GGATC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 94
BspTI CTTAAG 1 cut(s) 272
BsrI ACTGG 2 cut(s) 75, 204
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 1 cut(s) 191
BssSI CACGAG 1 cut(s) 173
Bst2BI CACGAG 1 cut(s) 173
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 1 cut(s) 349
BstAFI CTTAAG 1 cut(s) 272
BstC8I GCNNGC 2 cut(s) 160, 337
BstDEI CTNAG 1 cut(s) 43
BstFNI CGCG 1 cut(s) 269
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 1 cut(s) 194
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 1 cut(s) 191
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 90, 355
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 1 cut(s) 235
BstV2I GAAGAC 1 cut(s) 31
BsuRI GGCC 2 cut(s) 162, 278
BtrI CACGTC 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 218
BtsIMutI CAGTG 1 cut(s) 218
Cac8I GCNNGC 2 cut(s) 160, 337
CaiI CAGNNNCTG 1 cut(s) 210
CfoI GCGC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 95
CviJI RGCY 7 cut(s) 5, 113, 162, 231, 236, 248, 278
CviKI_1 RGCY 7 cut(s) 5, 113, 162, 231, 236, 248, 278
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 1 cut(s) 43
DpnI GATC 1 cut(s) 193
DpnII GATC 1 cut(s) 191
DraI TTTAAA 1 cut(s) 364
Eco147I AGGCCT 1 cut(s) 162
Eco57I CTGAAG 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 355
FaiI YATR 1 cut(s) 116
FaqI GGGAC 3 cut(s) 183, 192, 335
Fnu4HI GCNGC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 249
GlaI GCGC 1 cut(s) 270
GluI GCNGC 1 cut(s) 249
HaeIII GGCC 2 cut(s) 162, 278
HapII CCGG 1 cut(s) 92
HhaI GCGC 1 cut(s) 271
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HinfI GANTC 2 cut(s) 134, 171
HpaII CCGG 1 cut(s) 92
Hpy166II GTNNAC 2 cut(s) 178, 218
Hpy188III TCNNGA 1 cut(s) 175
Hpy8I GTNNAC 2 cut(s) 178, 218
HpyAV CCTTC 5 cut(s) 41, 59, 173, 221, 238
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 2 cut(s) 180, 303
HpyCH4V TGCA 4 cut(s) 26, 158, 218, 264
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 1 cut(s) 43
HpySE526I ACGT 2 cut(s) 180, 303
HspAI GCGC 1 cut(s) 269
KpnI GGTACC 1 cut(s) 98
Kzo9I GATC 1 cut(s) 191
Lsp1109I GCAGC 1 cut(s) 235
MaeII ACGT 2 cut(s) 180, 303
MalI GATC 1 cut(s) 193
MboI GATC 1 cut(s) 191
MboII GAAGA 1 cut(s) 31
MfeI CAATTG 1 cut(s) 309
MhlI GDGCHC 2 cut(s) 220, 288
MluCI AATT 3 cut(s) 186, 309, 319
MlyI GAGTC 2 cut(s) 128, 165
MnlI CCTC 1 cut(s) 363
MseI TTAA 5 cut(s) 86, 183, 273, 294, 363
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 2 cut(s) 92, 357
MunI CAATTG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 357
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 275
NciI CCSGG 1 cut(s) 92
NdeII GATC 1 cut(s) 191
NlaIV GGNNCC 1 cut(s) 96
PceI AGGCCT 1 cut(s) 162
PkrI GCNGC 1 cut(s) 250
PleI GAGTC 2 cut(s) 128, 165
PpsI GAGTC 2 cut(s) 128, 165
Psp1406I AACGTT 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 1 cut(s) 96
PstNI CAGNNNCTG 1 cut(s) 210
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
SaqAI TTAA 5 cut(s) 86, 183, 273, 294, 363
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 1 cut(s) 191
SchI GAGTC 2 cut(s) 128, 165
ScrFI CCNGG 2 cut(s) 92, 357
SduI GDGCHC 2 cut(s) 220, 288
SetI ASST 3 cut(s) 115, 183, 306
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 3 cut(s) 186, 309, 319
SseBI AGGCCT 1 cut(s) 162
SsiI CCGC 1 cut(s) 267
StuI AGGCCT 1 cut(s) 162
StyD4I CCNGG 2 cut(s) 90, 355
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 2 cut(s) 183, 306
TasI AATT 3 cut(s) 186, 309, 319
Tru1I TTAA 5 cut(s) 86, 183, 273, 294, 363
Tru9I TTAA 5 cut(s) 86, 183, 273, 294, 363
TscAI CASTG 1 cut(s) 225
TseI GCWGC 1 cut(s) 248
TspRI CASTG 1 cut(s) 225
Vha464I CTTAAG 1 cut(s) 272
VneI GTGCAC 1 cut(s) 216
XapI RAATTY 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.