Rh5AG213300

14 kDa proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018613
Physical Location & Seq
Forward (+)
25160377 .. 25160745
369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG213300.1

Sequence Viewer

Length: 369 bp
ATGGCTTCTTCTACCAATCTCTCTGCAACCCTTCTCGTCTTCTCAGTCCTTCTCTACCCACCACCAATACTGGCACAAACACCAATAACACCGGTACCATCAACACCAGCACCAGCTATGGAAACTTGCCCGAGAGACTCGCTGAAGTTAGGGGTTTGTGCAGGCCTTCTGGGACTCGTGAACGTTAAAATTGGATCCAGTCCCTGCTGTGCACTGCTTGAAGGCTTGGCTGATTTGAAGGCTGCTCTTTGTCTTTGCACCACGCTTAAGGCCAATGTGCTTGGTATTAACTTGGACGTGCCAATTGCTTTGAATTTGATTGTTAGTGCTTGCCAGAAAACTGTCCCTCCTGGTTTTAAATGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

122

Amino Acids

12.49

Weight (kDa)

7.48

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 35 - 121 4.3e-07 Probable lipid transfer
Hydrophob_seed PF14547 42 - 121 1.3e-24 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 43 - 121 5.1e-13 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018613)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0030791
rosa_laevigata RLG00000033253
rosa_multiflora Rmu_co8328467.1_g000001
rosa_roxburghii Rroxscaffold_1G00049500
rosa_rugosa Rorug05G0120800
rosa_samantha Rh5AG213300 Rh5BG212100 Rh5CG235400 Rh5DG216900
rosa_wichuraiana Rw5G019510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 94
AccB1I GGYRCC 1 cut(s) 94
AclI AACGTT 1 cut(s) 183
AclWI GGATC 2 cut(s) 189, 202
AcsI RAATTY 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 1 cut(s) 96
AflII CTTAAG 1 cut(s) 266
AgeI ACCGGT 1 cut(s) 91
AgsI TTSAA 3 cut(s) 221, 238, 313
AjiI CACGTC 1 cut(s) 298
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 129
Alw44I GTGCAC 1 cut(s) 210
AlwI GGATC 2 cut(s) 189, 202
AlwNI CAGNNNCTG 1 cut(s) 204
Ama87I CYCGRG 1 cut(s) 130
AoxI GGCC 2 cut(s) 163, 270
ApaLI GTGCAC 1 cut(s) 210
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 1 cut(s) 313
AsiGI ACCGGT 1 cut(s) 91
Asp718I GGTACC 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 214
BaeI ACNNNNGTAYC 2 cut(s) 78, 111
BamHI GGATCC 1 cut(s) 194
BanI GGYRCC 1 cut(s) 94
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 1 cut(s) 229
BccI CCATC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 351
BcoDI GTCTC 1 cut(s) 129
BfrI CTTAAG 1 cut(s) 266
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 351
BmeT110I CYCGRG 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 298
BmiI GGNNCC 2 cut(s) 96, 196
BmrFI CCNGG 1 cut(s) 351
BpiI GAAGAC 1 cut(s) 31
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 75, 198
BseBI CCWGG 1 cut(s) 351
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 2 cut(s) 75, 198
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 1 cut(s) 229
BsgI GTGCAG 1 cut(s) 180
BshFI GGCC 2 cut(s) 165, 272
BshNI GGYRCC 1 cut(s) 94
BshTI ACCGGT 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 214
BsiHKCI CYCGRG 1 cut(s) 130
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 3 cut(s) 186, 186, 329
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 3 cut(s) 186, 186, 329
BsnI GGCC 2 cut(s) 165, 272
BsoBI CYCGRG 1 cut(s) 130
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 1 cut(s) 194
BspANI GGCC 2 cut(s) 165, 272
BspCNI CTCAG 1 cut(s) 56
BspLI GGNNCC 2 cut(s) 96, 196
BspPI GGATC 2 cut(s) 189, 202
BspT107I GGYRCC 1 cut(s) 94
BspTI CTTAAG 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 2 cut(s) 75, 198
BssAI RCCGGY 1 cut(s) 91
BssMI GATC 1 cut(s) 194
BssSI CACGAG 1 cut(s) 176
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 343
BstAFI CTTAAG 1 cut(s) 266
BstC8I GCNNGC 2 cut(s) 163, 331
BstDEI CTNAG 1 cut(s) 43
BstKTI GATC 1 cut(s) 197
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 1 cut(s) 194
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 349
BstSLI GKGCMC 1 cut(s) 214
BstV1I GCAGC 1 cut(s) 229
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
BsuRI GGCC 2 cut(s) 165, 272
BtrI CACGTC 1 cut(s) 298
BtsI GCAGTG 1 cut(s) 212
BtsIMutI CAGTG 1 cut(s) 212
Cac8I GCNNGC 2 cut(s) 163, 331
CaiI CAGNNNCTG 1 cut(s) 204
Cfr10I RCCGGY 1 cut(s) 91
Csp6I GTAC 1 cut(s) 95
CspAI ACCGGT 1 cut(s) 91
CviJI RGCY 7 cut(s) 5, 116, 165, 225, 230, 242, 272
CviKI_1 RGCY 7 cut(s) 5, 116, 165, 225, 230, 242, 272
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 1 cut(s) 43
DpnI GATC 1 cut(s) 196
DpnII GATC 1 cut(s) 194
DraI TTTAAA 1 cut(s) 358
Eco147I AGGCCT 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 349
FaiI YATR 1 cut(s) 119
FaqI GGGAC 3 cut(s) 186, 186, 329
Fnu4HI GCNGC 1 cut(s) 243
Fsp4HI GCNGC 1 cut(s) 243
GluI GCNGC 1 cut(s) 243
HaeIII GGCC 2 cut(s) 165, 272
HapII CCGG 1 cut(s) 92
HinfI GANTC 2 cut(s) 137, 174
HpaII CCGG 1 cut(s) 92
Hpy166II GTNNAC 2 cut(s) 181, 212
Hpy188III TCNNGA 1 cut(s) 178
Hpy8I GTNNAC 2 cut(s) 181, 212
HpyAV CCTTC 5 cut(s) 41, 59, 176, 215, 232
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4IV ACGT 2 cut(s) 183, 297
HpyCH4V TGCA 4 cut(s) 26, 161, 212, 258
HpyF3I CTNAG 1 cut(s) 43
HpySE526I ACGT 2 cut(s) 183, 297
KpnI GGTACC 1 cut(s) 98
Kzo9I GATC 1 cut(s) 194
Lsp1109I GCAGC 1 cut(s) 229
MaeII ACGT 2 cut(s) 183, 297
MalI GATC 1 cut(s) 196
MboI GATC 1 cut(s) 194
MboII GAAGA 1 cut(s) 31
MfeI CAATTG 1 cut(s) 303
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 3 cut(s) 189, 303, 313
MlyI GAGTC 2 cut(s) 131, 168
MnlI CCTC 1 cut(s) 357
MseI TTAA 4 cut(s) 186, 267, 288, 357
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 303
MvaI CCWGG 1 cut(s) 351
NdeII GATC 1 cut(s) 194
NlaIV GGNNCC 2 cut(s) 96, 196
PceI AGGCCT 1 cut(s) 165
PinAI ACCGGT 1 cut(s) 91
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 2 cut(s) 131, 168
PpsI GAGTC 2 cut(s) 131, 168
Psp1406I AACGTT 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 2 cut(s) 96, 196
PstNI CAGNNNCTG 1 cut(s) 204
PsuI RGATCY 1 cut(s) 194
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
SaqAI TTAA 4 cut(s) 186, 267, 288, 357
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 1 cut(s) 194
SchI GAGTC 2 cut(s) 131, 168
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 1 cut(s) 214
SetI ASST 3 cut(s) 118, 186, 300
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 3 cut(s) 189, 303, 313
SseBI AGGCCT 1 cut(s) 165
StuI AGGCCT 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 349
TaaI ACNGT 1 cut(s) 343
TaiI ACGT 2 cut(s) 186, 300
TasI AATT 3 cut(s) 189, 303, 313
Tru1I TTAA 4 cut(s) 186, 267, 288, 357
Tru9I TTAA 4 cut(s) 186, 267, 288, 357
TscAI CASTG 1 cut(s) 219
TseI GCWGC 1 cut(s) 242
TspRI CASTG 1 cut(s) 219
Vha464I CTTAAG 1 cut(s) 266
VneI GTGCAC 1 cut(s) 210
XapI RAATTY 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.