RLG00000033253

14 kDa proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018613
Physical Location & Seq
Forward (+)
24996457 .. 24999389
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033253

Sequence Viewer

Length: 621 bp
ATGGCTTCTTCTACCAATCTCTCTGCAACCCTGCTCGTCTTCTCAGTCCTTCTCTACCCACCACCAATACTGGCACAAACACCAATAACACCGGTACCAACAACAGCACCAGCTATGGAAACTTGCCCGAGAGACTCGCTGAAGTTAGGGGTTTGTGCTGGCCTTCTGGGACTCGTGAACGTTAAAATTGGATCGGCAGCAACCAGTCCCTGCTGTGCACTGCTTGAAGGCTTGGCTGATTTGAAGGCTGCTCTTTGTCTTTGCACCGCGCTGAAGGCCAATGTGCTTGGTATTAACTTGGACGTGCCAATTGCTTTGAATTTGCTTGTTAGTGCTTGCCAGAAAACTGTCCCTCCTGGTTTTAAATATGCGAAGGATGGCGGCGTCAGCCACAATTCTCCAAGCTACACGCTCAATTTTGAGGACGACACCACATGTGTCGATGACATCCTCCTCCAGTCGCAGCTGTTGGCCTTAGCCTCCTGGAGTTTGACCCAACCGGAGAAGTTGACCACGAGCTGGCGTATTCTGAACGACTTTCTAGAGGAAGATGAGGACAACCGGAGCTGGAGGTCACTGACGAGGAACAAAGCTAGAGGTCACCGAAGGCGAACAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

207

Amino Acids

22.12

Weight (kDa)

6.7

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 30 - 120 5.3e-06 Probable lipid transfer
Hydrophob_seed PF14547 41 - 122 1e-25 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 42 - 121 1.3e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018613)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0030791
rosa_laevigata RLG00000033253
rosa_multiflora Rmu_co8328467.1_g000001
rosa_roxburghii Rroxscaffold_1G00049500
rosa_rugosa Rorug05G0120800
rosa_samantha Rh5AG213300 Rh5BG212100 Rh5CG235400 Rh5DG216900
rosa_wichuraiana Rw5G019510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 94
AccB1I GGYRCC 1 cut(s) 94
AccB7I CCANNNNNTGG 1 cut(s) 519
AccII CGCG 1 cut(s) 269
AciI CCGC 2 cut(s) 267, 381
AclI AACGTT 1 cut(s) 180
AclWI GGATC 1 cut(s) 199
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 2 cut(s) 161, 293
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 1 cut(s) 96
AfiI CCNNNNNNNGG 1 cut(s) 519
AflIII ACRYGT 1 cut(s) 434
AgeI ACCGGT 1 cut(s) 91
AgsI TTSAA 3 cut(s) 227, 244, 319
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 2 cut(s) 355, 482
AluBI AGCT 6 cut(s) 113, 405, 466, 519, 567, 593
AluI AGCT 6 cut(s) 113, 405, 466, 519, 567, 593
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 1 cut(s) 126
Alw44I GTGCAC 1 cut(s) 216
AlwI GGATC 1 cut(s) 199
AlwNI CAGNNNCTG 1 cut(s) 210
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 3 cut(s) 160, 276, 471
ApaLI GTGCAC 1 cut(s) 216
ApeKI GCWGC 3 cut(s) 197, 248, 463
ApoI RAATTY 1 cut(s) 319
AsiGI ACCGGT 1 cut(s) 91
Asp718I GGTACC 1 cut(s) 94
AspLEI GCGC 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 593
AvaI CYCGRG 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 220
BaeI ACNNNNGTAYC 2 cut(s) 78, 111
BanI GGYRCC 1 cut(s) 94
BauI CACGAG 2 cut(s) 173, 514
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 3 cut(s) 209, 235, 475
BccI CCATC 1 cut(s) 371
BciT130I CCWGG 2 cut(s) 357, 484
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 2 cut(s) 542, 594
BisI GCNGC 4 cut(s) 198, 249, 382, 464
BlsI GCNGC 4 cut(s) 199, 250, 383, 465
Bme1390I CCNGG 2 cut(s) 357, 484
BmeT110I CYCGRG 1 cut(s) 127
BmgBI CACGTC 1 cut(s) 304
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 2 cut(s) 357, 484
BpiI GAAGAC 1 cut(s) 31
BpmI CTGGAG 3 cut(s) 440, 505, 589
Bpu10I CCTNAGC 1 cut(s) 475
BsaHI GRCGYC 1 cut(s) 384
BsaWI WCCGGW 3 cut(s) 91, 499, 561
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 3 cut(s) 75, 204, 457
BseBI CCWGG 2 cut(s) 357, 484
BseGI GGATG 2 cut(s) 382, 447
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 3 cut(s) 75, 204, 457
BseRI GAGGAG 1 cut(s) 443
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 3 cut(s) 209, 235, 475
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 3 cut(s) 162, 278, 473
BshNI GGYRCC 1 cut(s) 94
BshTI ACCGGT 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 3 cut(s) 92, 500, 562
BslFI GGGAC 3 cut(s) 183, 192, 335
BslI CCNNNNNNNGG 1 cut(s) 519
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 3 cut(s) 183, 192, 335
BsnI GGCC 3 cut(s) 162, 278, 473
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 1 cut(s) 191
BspACI CCGC 2 cut(s) 267, 381
BspANI GGCC 3 cut(s) 162, 278, 473
BspCNI CTCAG 1 cut(s) 56
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 1 cut(s) 96
BspPI GGATC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 3 cut(s) 75, 204, 457
BssAI RCCGGY 1 cut(s) 91
BssMI GATC 1 cut(s) 191
BssNI GRCGYC 1 cut(s) 384
BssSI CACGAG 2 cut(s) 173, 514
Bst2BI CACGAG 2 cut(s) 173, 514
Bst2UI CCWGG 2 cut(s) 357, 484
Bst4CI ACNGT 1 cut(s) 349
BstACI GRCGYC 1 cut(s) 384
BstC8I GCNNGC 3 cut(s) 160, 337, 521
BstDEI CTNAG 2 cut(s) 43, 475
BstEII GGTNACC 1 cut(s) 599
BstF5I GGATG 2 cut(s) 382, 447
BstFNI CGCG 1 cut(s) 269
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 1 cut(s) 194
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 1 cut(s) 191
BstMWI GCNNNNNNNGC 2 cut(s) 275, 387
BstNI CCWGG 2 cut(s) 357, 484
BstNSI RCATGY 1 cut(s) 438
BstPI GGTNACC 1 cut(s) 599
BstSCI CCNGG 2 cut(s) 355, 482
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 3 cut(s) 209, 235, 475
BstV2I GAAGAC 1 cut(s) 31
BsuRI GGCC 3 cut(s) 162, 278, 473
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 2 cut(s) 382, 447
BtsI GCAGTG 1 cut(s) 218
BtsIMutI CAGTG 2 cut(s) 218, 575
Cac8I GCNNGC 3 cut(s) 160, 337, 521
CaiI CAGNNNCTG 1 cut(s) 210
CfoI GCGC 1 cut(s) 271
Cfr10I RCCGGY 1 cut(s) 91
CseI GACGC 1 cut(s) 373
Csp6I GTAC 1 cut(s) 95
CspAI ACCGGT 1 cut(s) 91
CviAII CATG 1 cut(s) 435
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 2 cut(s) 43, 475
DpnI GATC 1 cut(s) 193
DpnII GATC 1 cut(s) 191
DraI TTTAAA 1 cut(s) 364
Eco57I CTGAAG 2 cut(s) 161, 293
Eco88I CYCGRG 1 cut(s) 127
Eco91I GGTNACC 1 cut(s) 599
EcoO65I GGTNACC 1 cut(s) 599
EcoRII CCWGG 2 cut(s) 355, 482
FaeI CATG 1 cut(s) 438
FaiI YATR 3 cut(s) 116, 369, 436
FaqI GGGAC 3 cut(s) 183, 192, 335
FatI CATG 1 cut(s) 434
Fnu4HI GCNGC 4 cut(s) 198, 249, 382, 464
FokI GGATG 2 cut(s) 389, 434
Fsp4HI GCNGC 4 cut(s) 198, 249, 382, 464
FspBI CTAG 2 cut(s) 542, 594
GlaI GCGC 1 cut(s) 270
GluI GCNGC 4 cut(s) 198, 249, 382, 464
GsuI CTGGAG 3 cut(s) 440, 505, 589
HaeIII GGCC 3 cut(s) 162, 278, 473
HapII CCGG 3 cut(s) 92, 500, 562
HgaI GACGC 1 cut(s) 373
HhaI GCGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 384
Hin1II CATG 1 cut(s) 438
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HinfI GANTC 2 cut(s) 134, 171
HpaII CCGG 3 cut(s) 92, 500, 562
HphI GGTGA 1 cut(s) 593
Hpy166II GTNNAC 3 cut(s) 178, 218, 510
Hpy188I TCNGA 1 cut(s) 531
Hpy188III TCNNGA 2 cut(s) 175, 542
Hpy8I GTNNAC 3 cut(s) 178, 218, 510
HpyAV CCTTC 7 cut(s) 59, 173, 221, 238, 268, 367, 600
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 2 cut(s) 180, 303
HpyCH4V TGCA 3 cut(s) 26, 218, 264
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 387
HpyF3I CTNAG 2 cut(s) 43, 475
HpySE526I ACGT 2 cut(s) 180, 303
Hsp92I GRCGYC 1 cut(s) 384
Hsp92II CATG 1 cut(s) 438
HspAI GCGC 1 cut(s) 269
KpnI GGTACC 1 cut(s) 98
Kzo9I GATC 1 cut(s) 191
LmnI GCTCC 1 cut(s) 564
Lsp1109I GCAGC 3 cut(s) 209, 235, 475
MaeI CTAG 2 cut(s) 542, 594
MaeII ACGT 2 cut(s) 180, 303
MaeIII GTNAC 2 cut(s) 573, 599
MalI GATC 1 cut(s) 193
MboI GATC 1 cut(s) 191
MboII GAAGA 2 cut(s) 31, 560
MfeI CAATTG 1 cut(s) 309
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 5 cut(s) 186, 309, 319, 394, 415
MlyI GAGTC 2 cut(s) 128, 165
MseI TTAA 3 cut(s) 183, 294, 363
MspA1I CMGCKG 1 cut(s) 466
MspI CCGG 3 cut(s) 92, 500, 562
MspR9I CCNGG 2 cut(s) 357, 484
MunI CAATTG 1 cut(s) 309
MvaI CCWGG 2 cut(s) 357, 484
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 275, 387
NdeII GATC 1 cut(s) 191
NlaIII CATG 1 cut(s) 438
NlaIV GGNNCC 1 cut(s) 96
NmuCI GTSAC 2 cut(s) 573, 599
NspI RCATGY 1 cut(s) 438
PciI ACATGT 1 cut(s) 434
PflMI CCANNNNNTGG 1 cut(s) 519
PfoI TCCNGGA 1 cut(s) 482
PinAI ACCGGT 1 cut(s) 91
PkrI GCNGC 4 cut(s) 199, 250, 383, 465
PleI GAGTC 2 cut(s) 128, 165
PpsI GAGTC 2 cut(s) 128, 165
PscI ACATGT 1 cut(s) 434
Psp1406I AACGTT 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 355, 482
PspEI GGTNACC 1 cut(s) 599
PspGI CCWGG 2 cut(s) 355, 482
PspN4I GGNNCC 1 cut(s) 96
PstNI CAGNNNCTG 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 466
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
SaqAI TTAA 3 cut(s) 183, 294, 363
SatI GCNGC 4 cut(s) 198, 249, 382, 464
Sau3AI GATC 1 cut(s) 191
SchI GAGTC 2 cut(s) 128, 165
ScrFI CCNGG 2 cut(s) 357, 484
SduI GDGCHC 1 cut(s) 220
Sse9I AATT 5 cut(s) 186, 309, 319, 394, 415
SsiI CCGC 2 cut(s) 267, 381
SspMI CTAG 2 cut(s) 542, 594
StyD4I CCNGG 2 cut(s) 355, 482
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 2 cut(s) 183, 306
TaqI TCGA 1 cut(s) 441
TasI AATT 5 cut(s) 186, 309, 319, 394, 415
TauI GCSGC 1 cut(s) 384
Tru1I TTAA 3 cut(s) 183, 294, 363
Tru9I TTAA 3 cut(s) 183, 294, 363
TscAI CASTG 2 cut(s) 225, 582
TseFI GTSAC 2 cut(s) 573, 599
TseI GCWGC 3 cut(s) 197, 248, 463
Tsp45I GTSAC 2 cut(s) 573, 599
TspRI CASTG 2 cut(s) 225, 582
Van91I CCANNNNNTGG 1 cut(s) 519
VneI GTGCAC 1 cut(s) 216
XapI RAATTY 1 cut(s) 319
XbaI TCTAGA 1 cut(s) 541
XceI RCATGY 1 cut(s) 438
XspI CTAG 2 cut(s) 542, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.