Rw5G019510

14 kDa proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018613
Physical Location & Seq
Forward (+)
25454788 .. 25455165
378 bp
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UTR
Exon/CDS
Intron
Rw5G019510.1

Sequence Viewer

Length: 378 bp
ATGGCTTCTTCTACCAATCTCTCTGCAACCCTTCTCGTCTTCTCAGTCCTTCTCTACCCACCACCAATACTGGCACAAACACCAATAACACCGGTACCATCAACACCAGCACCAGCTATGGAAACTTGCCCGAGAGACTCGCTGAAGTTAGGGGTTTGTGCAGGCCTTCTGGGACTCGTGAACGTTAAAATTGGATCGCTAGCAAGCAGTCCCTGCTGTGCACTGCTTGGAGGCTTGGCTGATTTGAAGGCTGCTCTTTGTCTTTGCACCACGCTTAAGGCCAATGTGCTTGGTATTAACTTGGACGTGCCAATTGCTTTGAATTTGATTGTTAGTGCTTGCCAGAAAACTGTCCCTCCTGGTTTTAAATGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

125

Amino Acids

12.69

Weight (kDa)

8.14

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 35 - 124 2.9e-06 Probable lipid transfer
Hydrophob_seed PF14547 42 - 124 4.2e-26 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 43 - 124 5.5e-13 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018613)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0030791
rosa_laevigata RLG00000033253
rosa_multiflora Rmu_co8328467.1_g000001
rosa_roxburghii Rroxscaffold_1G00049500
rosa_rugosa Rorug05G0120800
rosa_samantha Rh5AG213300 Rh5BG212100 Rh5CG235400 Rh5DG216900
rosa_wichuraiana Rw5G019510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 94
AccB1I GGYRCC 1 cut(s) 94
AclI AACGTT 1 cut(s) 183
AclWI GGATC 1 cut(s) 202
AcsI RAATTY 1 cut(s) 322
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 1 cut(s) 96
AflII CTTAAG 1 cut(s) 275
AgeI ACCGGT 1 cut(s) 91
AgsI TTSAA 2 cut(s) 247, 322
AjiI CACGTC 1 cut(s) 307
AjnI CCWGG 1 cut(s) 358
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 129
Alw44I GTGCAC 1 cut(s) 219
AlwI GGATC 1 cut(s) 202
AlwNI CAGNNNCTG 1 cut(s) 213
Ama87I CYCGRG 1 cut(s) 130
AoxI GGCC 2 cut(s) 163, 279
ApaLI GTGCAC 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 1 cut(s) 322
AsiGI ACCGGT 1 cut(s) 91
Asp718I GGTACC 1 cut(s) 94
AsuNHI GCTAGC 1 cut(s) 199
AvaI CYCGRG 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 223
BaeI ACNNNNGTAYC 2 cut(s) 78, 111
BanI GGYRCC 1 cut(s) 94
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 1 cut(s) 238
BccI CCATC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 360
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 1 cut(s) 200
BfrI CTTAAG 1 cut(s) 275
BisI GCNGC 1 cut(s) 252
BlsI GCNGC 1 cut(s) 253
Bme1390I CCNGG 1 cut(s) 360
BmeT110I CYCGRG 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 360
BmtI GCTAGC 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 31
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 360
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 1 cut(s) 75
BseSI GKGCMC 1 cut(s) 223
BseXI GCAGC 1 cut(s) 238
BsgI GTGCAG 1 cut(s) 180
BshFI GGCC 2 cut(s) 165, 281
BshNI GGYRCC 1 cut(s) 94
BshTI ACCGGT 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 130
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 3 cut(s) 186, 195, 338
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 3 cut(s) 186, 195, 338
BsnI GGCC 2 cut(s) 165, 281
BsoBI CYCGRG 1 cut(s) 130
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 1 cut(s) 194
BspANI GGCC 2 cut(s) 165, 281
BspCNI CTCAG 1 cut(s) 56
BspLI GGNNCC 1 cut(s) 96
BspOI GCTAGC 1 cut(s) 203
BspPI GGATC 1 cut(s) 202
BspT107I GGYRCC 1 cut(s) 94
BspTI CTTAAG 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 91
BssMI GATC 1 cut(s) 194
BssSI CACGAG 1 cut(s) 176
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 1 cut(s) 352
BstAFI CTTAAG 1 cut(s) 275
BstAPI GCANNNNNTGC 1 cut(s) 213
BstC8I GCNNGC 4 cut(s) 163, 201, 205, 340
BstDEI CTNAG 1 cut(s) 43
BstKTI GATC 1 cut(s) 197
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 1 cut(s) 194
BstMWI GCNNNNNNNGC 1 cut(s) 213
BstNI CCWGG 1 cut(s) 360
BstSCI CCNGG 1 cut(s) 358
BstSLI GKGCMC 1 cut(s) 223
BstV1I GCAGC 1 cut(s) 238
BstV2I GAAGAC 1 cut(s) 31
BsuRI GGCC 2 cut(s) 165, 281
BtrI CACGTC 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 221
BtsIMutI CAGTG 1 cut(s) 221
Cac8I GCNNGC 4 cut(s) 163, 201, 205, 340
CaiI CAGNNNCTG 1 cut(s) 213
Cfr10I RCCGGY 1 cut(s) 91
Csp6I GTAC 1 cut(s) 95
CspAI ACCGGT 1 cut(s) 91
CviJI RGCY 7 cut(s) 5, 116, 165, 234, 239, 251, 281
CviKI_1 RGCY 7 cut(s) 5, 116, 165, 234, 239, 251, 281
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 1 cut(s) 43
DpnI GATC 1 cut(s) 196
DpnII GATC 1 cut(s) 194
DraI TTTAAA 1 cut(s) 367
Eco147I AGGCCT 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 358
FaiI YATR 1 cut(s) 119
FaqI GGGAC 3 cut(s) 186, 195, 338
Fnu4HI GCNGC 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 252
FspBI CTAG 1 cut(s) 200
GluI GCNGC 1 cut(s) 252
HaeIII GGCC 2 cut(s) 165, 281
HapII CCGG 1 cut(s) 92
HinfI GANTC 2 cut(s) 137, 174
HpaII CCGG 1 cut(s) 92
Hpy166II GTNNAC 2 cut(s) 181, 221
Hpy188III TCNNGA 1 cut(s) 178
Hpy8I GTNNAC 2 cut(s) 181, 221
HpyAV CCTTC 4 cut(s) 41, 59, 176, 241
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4IV ACGT 2 cut(s) 183, 306
HpyCH4V TGCA 4 cut(s) 26, 161, 221, 267
HpyF10VI GCNNNNNNNGC 1 cut(s) 213
HpyF3I CTNAG 1 cut(s) 43
HpySE526I ACGT 2 cut(s) 183, 306
KpnI GGTACC 1 cut(s) 98
Kzo9I GATC 1 cut(s) 194
Lsp1109I GCAGC 1 cut(s) 238
MaeI CTAG 1 cut(s) 200
MaeII ACGT 2 cut(s) 183, 306
MalI GATC 1 cut(s) 196
MboI GATC 1 cut(s) 194
MboII GAAGA 1 cut(s) 31
MfeI CAATTG 1 cut(s) 312
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 3 cut(s) 189, 312, 322
MlyI GAGTC 2 cut(s) 131, 168
MnlI CCTC 2 cut(s) 224, 366
MseI TTAA 4 cut(s) 186, 276, 297, 366
MspCI CTTAAG 1 cut(s) 275
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 360
MunI CAATTG 1 cut(s) 312
MvaI CCWGG 1 cut(s) 360
MwoI GCNNNNNNNGC 1 cut(s) 213
NdeII GATC 1 cut(s) 194
NheI GCTAGC 1 cut(s) 199
NlaIV GGNNCC 1 cut(s) 96
PceI AGGCCT 1 cut(s) 165
PinAI ACCGGT 1 cut(s) 91
PkrI GCNGC 1 cut(s) 253
PleI GAGTC 2 cut(s) 131, 168
PpsI GAGTC 2 cut(s) 131, 168
Psp1406I AACGTT 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 358
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 1 cut(s) 96
PstNI CAGNNNCTG 1 cut(s) 213
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
SaqAI TTAA 4 cut(s) 186, 276, 297, 366
SatI GCNGC 1 cut(s) 252
Sau3AI GATC 1 cut(s) 194
SchI GAGTC 2 cut(s) 131, 168
ScrFI CCNGG 1 cut(s) 360
SduI GDGCHC 1 cut(s) 223
SetI ASST 3 cut(s) 118, 186, 309
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 3 cut(s) 189, 312, 322
SseBI AGGCCT 1 cut(s) 165
SspMI CTAG 1 cut(s) 200
StuI AGGCCT 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 358
TaaI ACNGT 1 cut(s) 352
TaiI ACGT 2 cut(s) 186, 309
TasI AATT 3 cut(s) 189, 312, 322
Tru1I TTAA 4 cut(s) 186, 276, 297, 366
Tru9I TTAA 4 cut(s) 186, 276, 297, 366
TscAI CASTG 1 cut(s) 228
TseI GCWGC 1 cut(s) 251
TspRI CASTG 1 cut(s) 228
Vha464I CTTAAG 1 cut(s) 275
VneI GTGCAC 1 cut(s) 219
XapI RAATTY 1 cut(s) 322
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.