RchiOBHm_Chr4g0442841

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0019918
Physical Location & Seq
Reverse (-)
64288516 .. 64288779
264 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 264 bp
ATGCATGCATATCCATATTTTGCATACACTGCAGATCCTCTCAATGTCCAGTTGGATTATGCTTTGTTTAGGGCACCTTACCCTGTCGTTCAAGACAGTTCACTGGCAAATTACAACATGTTTGACGCTACGGTTGATGCTTTCTACTCGGCAATTGAGAAAGCTGGCAAGCCAGATGTTAATATCATAGTATCCAAGTCTGGTTGGCCTTCAGCTGGGAACGGAAATCTCACCACTCTCGAGCTTGACAGGACATACAAATGA

Protein Analysis

87

Amino Acids

9.67

Weight (kDa)

4.7

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 3 - 76 1.2e-20 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019918)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0442841
rosa_laevigata RLG00000005967
rosa_multiflora Rmu_co8050374.1_g000001 Rmu_sc0004359.1_g000031
rosa_samantha Rh2AG093300 Rh4AG396300 Rh4DG403100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AclWI GGATC 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 195
AfiI CCNNNNNNNGG 1 cut(s) 215
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 1 cut(s) 92
AluBI AGCT 3 cut(s) 164, 215, 244
AluI AGCT 3 cut(s) 164, 215, 244
AlwI GGATC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 1 cut(s) 206
AsuHPI GGTGA 1 cut(s) 223
AvaI CYCGRG 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 76
BanI GGYRCC 1 cut(s) 73
BciVI GTATCC 1 cut(s) 202
BfmI CTRYAG 1 cut(s) 30
BfuI GTATCC 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 75
BmsI GCATC 1 cut(s) 127
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 49, 108
BseLI CCNNNNNNNGG 1 cut(s) 215
BseNI ACTGG 2 cut(s) 49, 108
BseSI GKGCMC 1 cut(s) 76
BseYI CCCAGC 1 cut(s) 215
BshFI GGCC 1 cut(s) 208
BshNI GGYRCC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 215
BsnI GGCC 1 cut(s) 208
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 1 cut(s) 34
BspANI GGCC 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 75
BspMAI CTGCAG 1 cut(s) 34
BspPI GGATC 1 cut(s) 29
BspT107I GGYRCC 1 cut(s) 73
BsrI ACTGG 2 cut(s) 49, 108
BssMI GATC 1 cut(s) 34
Bst4CI ACNGT 2 cut(s) 98, 133
BstAPI GCANNNNNTGC 1 cut(s) 29
BstC8I GCNNGC 3 cut(s) 6, 166, 170
BstKTI GATC 1 cut(s) 37
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNSI RCATGY 2 cut(s) 8, 121
BstSFI CTRYAG 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuI GTATCC 1 cut(s) 202
BsuRI GGCC 1 cut(s) 208
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 2 cut(s) 27, 101
Cac8I GCNNGC 3 cut(s) 6, 166, 170
CseI GACGC 1 cut(s) 134
CviAII CATG 2 cut(s) 5, 118
CviJI RGCY 5 cut(s) 164, 172, 208, 215, 244
CviKI_1 RGCY 5 cut(s) 164, 172, 208, 215, 244
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 195
Eco88I CYCGRG 1 cut(s) 239
EcoT22I ATGCAT 2 cut(s) 6, 10
FaeI CATG 2 cut(s) 8, 121
FaiI YATR 8 cut(s) 6, 10, 16, 25, 60, 119, 188, 256
FatI CATG 2 cut(s) 4, 117
GsaI CCCAGC 1 cut(s) 219
HaeIII GGCC 1 cut(s) 208
HgaI GACGC 1 cut(s) 134
Hin1II CATG 2 cut(s) 8, 121
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 1 cut(s) 101
Hpy188III TCNNGA 2 cut(s) 92, 239
Hpy8I GTNNAC 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 219
HpyCH4III ACNGT 2 cut(s) 98, 133
HpyCH4V TGCA 4 cut(s) 4, 8, 23, 32
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
Hsp92II CATG 2 cut(s) 8, 121
Kzo9I GATC 1 cut(s) 34
LpnPI CCDG 8 cut(s) 62, 89, 96, 150, 186, 186, 201, 235
LweI GCATC 1 cut(s) 127
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 76
MluCI AATT 2 cut(s) 109, 153
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 1 cut(s) 48
Mph1103I ATGCAT 2 cut(s) 6, 10
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 1 cut(s) 259
MspA1I CMGCKG 1 cut(s) 215
MunI CAATTG 1 cut(s) 153
MwoI GCNNNNNNNGC 1 cut(s) 29
NdeII GATC 1 cut(s) 34
NlaIII CATG 2 cut(s) 8, 121
NlaIV GGNNCC 1 cut(s) 75
NmeAIII GCCGAG 1 cut(s) 128
NsiI ATGCAT 2 cut(s) 6, 10
NspI RCATGY 2 cut(s) 8, 121
PaeI GCATGC 1 cut(s) 8
PaeR7I CTCGAG 1 cut(s) 239
PciI ACATGT 1 cut(s) 117
PscI ACATGT 1 cut(s) 117
PspFI CCCAGC 1 cut(s) 215
PspN4I GGNNCC 1 cut(s) 75
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 215
RseI CAYNNNNRTG 1 cut(s) 259
SaqAI TTAA 1 cut(s) 180
Sau3AI GATC 1 cut(s) 34
SduI GDGCHC 1 cut(s) 76
SetI ASST 4 cut(s) 79, 166, 217, 246
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 30
Sfr274I CTCGAG 1 cut(s) 239
SlaI CTCGAG 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
SphI GCATGC 1 cut(s) 8
Sse9I AATT 2 cut(s) 109, 153
TaaI ACNGT 2 cut(s) 98, 133
TaqI TCGA 1 cut(s) 240
TasI AATT 2 cut(s) 109, 153
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 2 cut(s) 34, 108
TspGWI ACGGA 1 cut(s) 237
TspRI CASTG 2 cut(s) 34, 108
XceI RCATGY 2 cut(s) 8, 121
XhoI CTCGAG 1 cut(s) 239
Zsp2I ATGCAT 2 cut(s) 6, 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.