RchiOBHm_Chr3g0459411

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0021089
Physical Location & Seq
Forward (+)
7855257 .. 7855673
417 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
ATGAAAGGTGAAGATATGAGCCTCCATACCGTGGGTGGAAGTTCTTCTATTGAATGGGTACAAGGATCACTATTGGCTCAAAATCAACCCCTTGCATGGTACAAGGCTATTTTAGATGCACCACCAGGAAATGCACCATTAGCTTTAGATATGGGTAGCATGGGAAAGGGTCAAATGTGGATTAATGGTCGGAGCATCGGACGCCATTGGCCAGCATATACAGCAAAAGGCACCTGTGGTACTTGTTATTATGCTGGAACTTATACTGAAAACAAATGCCGAACAAATTGTGGCCAGCCTTCCCAGAGATGGTACCATGTTCCCCGCTCGTGGTTGAAGCCAAGTGGGAATTTATTGGTTGTGTTTGAAGAATGGGGTGGTGATCCGACTAAGATTGCTTTGGTAGCAAGAAGTTGA

Protein Analysis

138

Amino Acids

15.12

Weight (kDa)

8.96

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BetaGal_gal-bd PF21467 31 - 117 3.9e-25 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021089)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g35280 FvH4_7g01440
rosa_chinensis RchiOBHm_Chr3g0459411
rosa_laevigata RLG00000025080
rosa_multiflora Rmu_co8266025.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 2 cut(s) 230, 312
AccB7I CCANNNNNTGG 1 cut(s) 31
AccBSI CCGCTC 1 cut(s) 327
AciI CCGC 1 cut(s) 325
AclWI GGATC 2 cut(s) 73, 377
AcoI YGGCCR 2 cut(s) 209, 292
AcsI RAATTY 1 cut(s) 349
AcyI GRCGYC 1 cut(s) 202
AfaI GTAC 4 cut(s) 60, 101, 241, 314
AfiI CCNNNNNNNGG 4 cut(s) 31, 96, 309, 330
AgsI TTSAA 3 cut(s) 53, 337, 368
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 1 cut(s) 143
AluI AGCT 1 cut(s) 143
AlwI GGATC 2 cut(s) 73, 377
AoxI GGCC 2 cut(s) 209, 292
ApoI RAATTY 1 cut(s) 349
AseI ATTAAT 1 cut(s) 183
Asp700I GAANNNNTTC 1 cut(s) 43
Asp718I GGTACC 1 cut(s) 312
AsuHPI GGTGA 2 cut(s) 20, 392
BalI TGGCCA 2 cut(s) 211, 294
BanI GGYRCC 2 cut(s) 230, 312
BauI CACGAG 1 cut(s) 328
BccI CCATC 1 cut(s) 303
BciT130I CCWGG 1 cut(s) 126
Bme1390I CCNGG 1 cut(s) 126
BmiI GGNNCC 2 cut(s) 232, 314
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 2 cut(s) 106, 204
BsaHI GRCGYC 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 30
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 96, 309, 330
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 30
BseLI CCNNNNNNNGG 4 cut(s) 31, 96, 309, 330
BshFI GGCC 2 cut(s) 211, 294
BshNI GGYRCC 2 cut(s) 230, 312
BslI CCNNNNNNNGG 4 cut(s) 31, 96, 309, 330
BsnI GGCC 2 cut(s) 211, 294
Bsp143I GATC 2 cut(s) 65, 382
BspACI CCGC 1 cut(s) 325
BspANI GGCC 2 cut(s) 211, 294
BspLI GGNNCC 2 cut(s) 232, 314
BspPI GGATC 2 cut(s) 73, 377
BspT107I GGYRCC 2 cut(s) 230, 312
BsrBI CCGCTC 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 2 cut(s) 65, 382
BssNI GRCGYC 1 cut(s) 202
BssSI CACGAG 1 cut(s) 328
Bst2BI CACGAG 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 31
BstACI GRCGYC 1 cut(s) 202
BstC8I GCNNGC 2 cut(s) 213, 296
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 1 cut(s) 30
BstKTI GATC 2 cut(s) 68, 385
BstMBI GATC 2 cut(s) 65, 382
BstMWI GCNNNNNNNGC 4 cut(s) 140, 201, 221, 404
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 124
BsuRI GGCC 2 cut(s) 211, 294
BtgI CCRYGG 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 213, 296
CseI GACGC 1 cut(s) 210
Csp6I GTAC 4 cut(s) 59, 100, 240, 313
CviAII CATG 3 cut(s) 96, 160, 317
CviJI RGCY 8 cut(s) 21, 77, 107, 143, 211, 294, 298, 340
CviKI_1 RGCY 8 cut(s) 21, 77, 107, 143, 211, 294, 298, 340
CviQI GTAC 4 cut(s) 59, 100, 240, 313
DdeI CTNAG 1 cut(s) 390
DpnI GATC 2 cut(s) 67, 384
DpnII GATC 2 cut(s) 65, 382
EaeI YGGCCR 2 cut(s) 209, 292
EcoRII CCWGG 1 cut(s) 124
FaeI CATG 3 cut(s) 99, 163, 320
FatI CATG 3 cut(s) 95, 159, 316
FauI CCCGC 1 cut(s) 332
HaeIII GGCC 2 cut(s) 211, 294
HgaI GACGC 1 cut(s) 210
Hin1I GRCGYC 1 cut(s) 202
Hin1II CATG 3 cut(s) 99, 163, 320
HphI GGTGA 2 cut(s) 20, 392
Hpy188I TCNGA 3 cut(s) 192, 200, 387
HpyAV CCTTC 1 cut(s) 309
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 3 cut(s) 95, 119, 134
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 201, 221, 404
HpyF3I CTNAG 1 cut(s) 390
Hsp92I GRCGYC 1 cut(s) 202
Hsp92II CATG 3 cut(s) 99, 163, 320
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 2 cut(s) 65, 382
LmnI GCTCC 1 cut(s) 192
LpnPI CCDG 7 cut(s) 111, 138, 225, 240, 247, 308, 317
LweI GCATC 2 cut(s) 106, 204
MalI GATC 2 cut(s) 67, 384
MbiI CCGCTC 1 cut(s) 327
MboI GATC 2 cut(s) 65, 382
MboII GAAGA 3 cut(s) 23, 36, 380
MlsI TGGCCA 2 cut(s) 211, 294
MluCI AATT 2 cut(s) 286, 349
MluNI TGGCCA 2 cut(s) 211, 294
MmeI TCCRAC 2 cut(s) 170, 410
MnlI CCTC 1 cut(s) 32
Mox20I TGGCCA 2 cut(s) 211, 294
MroXI GAANNNNTTC 1 cut(s) 43
MscI TGGCCA 2 cut(s) 211, 294
MseI TTAA 1 cut(s) 183
Msp20I TGGCCA 2 cut(s) 211, 294
MspR9I CCNGG 1 cut(s) 126
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 4 cut(s) 140, 201, 221, 404
NdeII GATC 2 cut(s) 65, 382
NlaIII CATG 3 cut(s) 99, 163, 320
NlaIV GGNNCC 2 cut(s) 232, 314
PdmI GAANNNNTTC 1 cut(s) 43
PflMI CCANNNNNTGG 1 cut(s) 31
PshBI ATTAAT 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 232, 314
RsaI GTAC 4 cut(s) 60, 101, 241, 314
RsaNI GTAC 4 cut(s) 59, 100, 240, 313
SaqAI TTAA 1 cut(s) 183
Sau3AI GATC 2 cut(s) 65, 382
ScrFI CCNGG 1 cut(s) 126
SetI ASST 3 cut(s) 10, 145, 236
SfaNI GCATC 2 cut(s) 106, 204
Sse9I AATT 2 cut(s) 286, 349
SsiI CCGC 1 cut(s) 325
StyD4I CCNGG 1 cut(s) 124
TaaI ACNGT 1 cut(s) 31
TasI AATT 2 cut(s) 286, 349
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TspDTI ATGAA 1 cut(s) 17
Van91I CCANNNNNTGG 1 cut(s) 31
VspI ATTAAT 1 cut(s) 183
XapI RAATTY 1 cut(s) 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 32
XmnI GAANNNNTTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.