RLG00000025080

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0021089
Physical Location & Seq
Reverse (-)
41828740 .. 41829534
795 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025080

Sequence Viewer

Length: 450 bp
ATGTCACGATGGCAATGGACCTATAAGATTGGTATGAAAGGTGAAGATATGAGCCTCCATACCGTGGGTGGAAGTTCTTCTATTGAATGGGTACAAGGATCACTATTGGCTCAAAATCAACCCCTTACACGGTACAAGGGTATTTTAGATGCACCACCGGGAAATGCACCAGTAGCTTTAGATATGGGTAGCATGGGAAAGGGTCAAATGTGGATTAATGGTCGGAGCATCGCACGCCATTGGCCAGCATATACAGCAAAAGGCACCTGTGGTACTTGTCATTATGCTGCAACTTATACTGAAAACAAATGCCGAACAAATTGTGGCCATCCTTCCCAGAGATGGTACCATGTTCCCCGCTCGTGGTTGAAGCCAAGTGGGAATTTATTGGTTGTGTTTGAAGAATGGGGTGGTGATCCGACTAAGATTGCTTTGGTAGCAAGAAGTTGA

Protein Analysis

150

Amino Acids

16.54

Weight (kDa)

9.44

Isoelectric Point (pI)

52.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BetaGal_gal-bd PF21467 43 - 128 5.6e-22 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021089)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g35280 FvH4_7g01440
rosa_chinensis RchiOBHm_Chr3g0459411
rosa_laevigata RLG00000025080
rosa_multiflora Rmu_co8266025.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 345
AccB1I GGYRCC 2 cut(s) 263, 345
AccB7I CCANNNNNTGG 1 cut(s) 64
AccBSI CCGCTC 1 cut(s) 360
AciI CCGC 1 cut(s) 358
AclWI GGATC 2 cut(s) 106, 410
AcoI YGGCCR 2 cut(s) 242, 325
AcsI RAATTY 1 cut(s) 382
AfaI GTAC 4 cut(s) 93, 134, 274, 347
AfiI CCNNNNNNNGG 4 cut(s) 64, 129, 342, 363
AgsI TTSAA 3 cut(s) 86, 370, 401
AluBI AGCT 1 cut(s) 176
AluI AGCT 1 cut(s) 176
AlwI GGATC 2 cut(s) 106, 410
AoxI GGCC 2 cut(s) 242, 325
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 382
AseI ATTAAT 1 cut(s) 216
Asp700I GAANNNNTTC 1 cut(s) 76
Asp718I GGTACC 1 cut(s) 345
AspS9I GGNCC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 2 cut(s) 53, 425
AvaII GGWCC 1 cut(s) 18
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BalI TGGCCA 2 cut(s) 244, 327
BanI GGYRCC 2 cut(s) 263, 345
BauI CACGAG 1 cut(s) 361
BbvI GCAGC 1 cut(s) 274
BccI CCATC 3 cut(s) 3, 336, 336
BcnI CCSGG 1 cut(s) 159
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 2 cut(s) 265, 347
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 2 cut(s) 139, 237
BpuMI CCSGG 1 cut(s) 159
BsaJI CCNNGG 1 cut(s) 63
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 129, 342, 363
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 20
BseDI CCNNGG 1 cut(s) 63
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 4 cut(s) 64, 129, 342, 363
BseMI GCAATG 1 cut(s) 20
BseNI ACTGG 1 cut(s) 170
BseXI GCAGC 1 cut(s) 274
BshFI GGCC 2 cut(s) 244, 327
BshNI GGYRCC 2 cut(s) 263, 345
BsiSI CCGG 1 cut(s) 158
BslI CCNNNNNNNGG 4 cut(s) 64, 129, 342, 363
BsnI GGCC 2 cut(s) 244, 327
Bsp143I GATC 2 cut(s) 98, 415
BspACI CCGC 1 cut(s) 358
BspANI GGCC 2 cut(s) 244, 327
BspLI GGNNCC 2 cut(s) 265, 347
BspPI GGATC 2 cut(s) 106, 410
BspT107I GGYRCC 2 cut(s) 263, 345
BsrBI CCGCTC 1 cut(s) 360
BsrDI GCAATG 1 cut(s) 20
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 63
BssMI GATC 2 cut(s) 98, 415
BssSI CACGAG 1 cut(s) 361
Bst2BI CACGAG 1 cut(s) 361
Bst4CI ACNGT 2 cut(s) 64, 132
BstC8I GCNNGC 2 cut(s) 235, 246
BstDEI CTNAG 1 cut(s) 423
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 1 cut(s) 328
BstKTI GATC 2 cut(s) 101, 418
BstMBI GATC 2 cut(s) 98, 415
BstMWI GCNNNNNNNGC 4 cut(s) 173, 234, 254, 437
BstSCI CCNGG 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 274
BsuRI GGCC 2 cut(s) 244, 327
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 214
BtsCI GGATG 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 235, 246
Cfr13I GGNCC 1 cut(s) 18
Csp6I GTAC 4 cut(s) 92, 133, 273, 346
CviAII CATG 2 cut(s) 193, 350
CviJI RGCY 6 cut(s) 54, 110, 176, 244, 327, 373
CviKI_1 RGCY 6 cut(s) 54, 110, 176, 244, 327, 373
CviQI GTAC 4 cut(s) 92, 133, 273, 346
DdeI CTNAG 1 cut(s) 423
DpnI GATC 2 cut(s) 100, 417
DpnII GATC 2 cut(s) 98, 415
EaeI YGGCCR 2 cut(s) 242, 325
Eco47I GGWCC 1 cut(s) 18
FaeI CATG 2 cut(s) 196, 353
FatI CATG 2 cut(s) 192, 349
FauI CCCGC 1 cut(s) 365
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 1 cut(s) 315
Fsp4HI GCNGC 1 cut(s) 288
GluI GCNGC 1 cut(s) 288
HaeIII GGCC 2 cut(s) 244, 327
HapII CCGG 1 cut(s) 158
Hin1II CATG 2 cut(s) 196, 353
HpaII CCGG 1 cut(s) 158
HphI GGTGA 2 cut(s) 53, 425
Hpy188I TCNGA 2 cut(s) 225, 420
Hpy188III TCNNGA 1 cut(s) 6
HpyAV CCTTC 1 cut(s) 342
HpyCH4III ACNGT 2 cut(s) 64, 132
HpyCH4V TGCA 3 cut(s) 152, 167, 290
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 234, 254, 437
HpyF3I CTNAG 1 cut(s) 423
Hsp92II CATG 2 cut(s) 196, 353
KpnI GGTACC 1 cut(s) 349
Kzo9I GATC 2 cut(s) 98, 415
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 5 cut(s) 171, 183, 258, 280, 350
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 2 cut(s) 139, 237
MaeIII GTNAC 1 cut(s) 3
MalI GATC 2 cut(s) 100, 417
MbiI CCGCTC 1 cut(s) 360
MboI GATC 2 cut(s) 98, 415
MboII GAAGA 3 cut(s) 56, 69, 413
MlsI TGGCCA 2 cut(s) 244, 327
MluCI AATT 2 cut(s) 319, 382
MluNI TGGCCA 2 cut(s) 244, 327
MmeI TCCRAC 2 cut(s) 203, 443
MnlI CCTC 1 cut(s) 65
Mox20I TGGCCA 2 cut(s) 244, 327
MroXI GAANNNNTTC 1 cut(s) 76
MscI TGGCCA 2 cut(s) 244, 327
MseI TTAA 1 cut(s) 216
Msp20I TGGCCA 2 cut(s) 244, 327
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 1 cut(s) 159
MwoI GCNNNNNNNGC 4 cut(s) 173, 234, 254, 437
NciI CCSGG 1 cut(s) 159
NdeII GATC 2 cut(s) 98, 415
NlaIII CATG 2 cut(s) 196, 353
NlaIV GGNNCC 2 cut(s) 265, 347
NmuCI GTSAC 1 cut(s) 3
PdmI GAANNNNTTC 1 cut(s) 76
PflMI CCANNNNNTGG 1 cut(s) 64
PkrI GCNGC 1 cut(s) 289
PshBI ATTAAT 1 cut(s) 216
PspN4I GGNNCC 2 cut(s) 265, 347
PspPI GGNCC 1 cut(s) 18
RsaI GTAC 4 cut(s) 93, 134, 274, 347
RsaNI GTAC 4 cut(s) 92, 133, 273, 346
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 2 cut(s) 98, 415
Sau96I GGNCC 1 cut(s) 18
ScrFI CCNGG 1 cut(s) 159
SetI ASST 4 cut(s) 23, 43, 178, 269
SfaNI GCATC 2 cut(s) 139, 237
SinI GGWCC 1 cut(s) 18
Sse9I AATT 2 cut(s) 319, 382
SsiI CCGC 1 cut(s) 358
StyD4I CCNGG 1 cut(s) 157
TaaI ACNGT 2 cut(s) 64, 132
TasI AATT 2 cut(s) 319, 382
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 50
Van91I CCANNNNNTGG 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 18
VspI ATTAAT 1 cut(s) 216
XapI RAATTY 1 cut(s) 382
XcmI CCANNNNNNNNNTGG 1 cut(s) 65
XmnI GAANNNNTTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.