RchiOBHm_Chr3g0461321

Protein MON2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007304
Physical Location & Seq
Forward (+)
9387177 .. 9413691
26515 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5007 bp
ATGGCTTTCATGGCGGTTTTGGAGTCAGATCTTCGCGCTCTCTCCGCCGAGGCTCGCCGTCGCTACCCTGCTGTCAAAGACGGCGCTGAGCACGCAATTATAAAGCTCCGTTCTTTGTCAAGCCCTGGTGAGATTGCGCAGAATGAGGACATATTGAGAATCTTCCTGATGGCTTGTGAGGTTAAAACTGTGAAGTTGAGTGTTATTGGACTCTCGTGTCTACAGAAGCTTATATCTCATGATGCTGTTGCTTCATCAGCTCTGCACGAGATACTTTGTACATTGAAGGATCATGCTGAAATGACCGATGAGAGTGTTCAACTTAAGACCCTGCAGACTGTATTGATAATATTGCAGTCACCTTTACACCCTCAGAATGAGGACGATATGGCTCAAGCTCTTGGTATATGCCTTCGGCTTCTCGAAAATAGGTCCCCTGACAGTGTGCGCAATACTGCTGCAGCTACTTTCAGGCAAGCTGTAGCACTGATTTTTGATCATGTGGTTTGCGCTGAGACTCTTCCTGCCGGCAAGCTTAGTTCTGGAGGTTACATCTCTCGGACCAGTCCAGTTAGTGGTGATGTTAGTTGCAGCATAAATCTTTCAGAGTCTTTGGATGGATCTGTTTCTGGACAGTCATCATTGATGAGGGAGACACTAACTAAAGCTGGAAAGCTTGGGCTTCGTCTGCTTGAAGACCTGACAGCTCTTGCTGCAGGTGGATCTGCAATCTGGTTACGTGTCAGTTCTCTACAGCGGTCCTTTGCACTTGACATACTGGATCCAGCCTTTTGGTTTGTTCTGTCCAACTATGTGGCTGTTTTCCGAACCTTGCTTCCTTATGAGCAGGTCCTGCGTTACCAGATATGTTCAATTCTAATGACCTCACTTCGTACAAATGCGGAGCTTGAAGGGGAAGCAGGAGAGCCGTCATTTCGACGCTTGGTCTTGCGGTCAGTTGCCCATATCATCCGGCTTTACAGTTCATCCCTTATCACTGAATGTGAGGTCTTCCTTAGTATGTTGGTGAAGGTCACATTTCTTGATTTGCCATTATGGCATCGTATTCTTGTTCTTGAGATCCTGAGGGGCTTTTGTGTGGAGGCAAGGACATTGCGGATTCTTTTCCGAAACTTTGATATGAATCCCAAGAACACCAACGTTGTGGAGGGCATGGTGAAAGCTCTTGCTAGAGTAGTTTCAAGTGTACAGGTTCAAGAGACTAGTGAAGAGAGCCTCGCAGCTGTTGCAGGAATGTTTAACAGCAAAGCTAAAGGAGTTGAGTGGAGCCTTGATAATGATGCATCCAATGCTGCAGTATTGGTTGCTAGTGAAGCTCATTGTATAACCTTGGCAGTCGAAGGTCTGCTCGGTGTAGTCTTCACAGTAGCTACATTGACGGATGAAGCAGTAGATTCCGGAGAGATTGAATCCCCCAGATGTGACTATGATCCACCTGCAAAGAAGACTGGAAATGCTGCTCTTCTCTGTCTCTCAATGGTTGATTCATTATGGTTGACGATACTAGATGCATTCTCCTTTATTTTGTCAAGGTCACAAGGTGAGGCTATTGTGTTGGAAATACTGAAGGGATATCAGGCCTTCACCCAGGCATGCGGAGTTCTTGGTGCTGTAGAACCTTTGAACTCCTTTTTAGCATCACTTTGCAAATTTACAATCATTTTCCCCGTTGAAGCAGAAAAAAGGAGCATTACTTTACAATCTCCTGGGTCCAAACGGTCTGAACAAGTAGTTGATCAAAGGGAAAGTGTTGTTCTCACCCCTAAGAATGTCCAGGCCTTAAGAACTCTCTTCAACATTGCTCATAGATTACACAATGTGTTGGGCCCATCCTGGGTTTTGGTATTAGAAACCCTAGCAGCCCTAGACCGCGCAATCCATTCTCCGCATGCTACCACACAGGAGGTGTCCACGGCTGTGCCAAAACTTACAAGAGAATCATCTGGTCAATCCAGTGACTTAAATATTCTTTCATCCTTGAATTCTCAGCTGTTTGAGAGCTCAGCACTGATGCACATATCTGCAGTAAAATCCCTTCTTTGTGCACTGGGCCAACTGTCACAGCAATGCATGGCTGGGATTTCAAATGGATCTGTACCTGCTTCAAGTCAAAAAGTTGGAAACATAAACTTTTCAGTGGAGAGAATGATATCCATCCTAGTCAATAATCTACACAGAGTGGAGCCATTGTGGGATCAAGTTGTTGGCCACTTTCTTGAGCTTGCAGAAAATTCTAACCAGCATTTACGTAACATGGCCCTTGATGCATTAGATGAATCAATATGTGCTGTTTTAGGTTCAGACAAGTTCCCAGACAACACAACAAGCAGATTCAATGGCTCATCTCAGAGTATGGATACTGGGGTTACAGATTTGGGATCACTTGAGTGTGCTGTCATATCTCCACTAAGGGTTCTTTATCTTTCTACTCAAAGTGTTGACTCACGTACTGGATCTTTGAAAATTCTTCTGCATGTTTTAGAGAGGCATGGGGAGAAATTACACTACAGTTGGCCAAATATTCTTGAAATGTTGAGGTCTGTAGCGGACTCTTCAGATAAGGAACTCATTACTCTGGGCTTTCAGTGCCTGCGGGTGATTATGAATGACGGACTTTCTACTATACCTGCAGACTGCCTTCAAGTATGTGTAGATGTGACTGGAGCATACAGTGCTCAAAAGACAGAGCTGAATATAAGTCTGACAGCAATAGGCCTCTTATGGACAACAACTGACTTTATTGTGAAAGCTCTAATCCATGGACCTGGGGCGGAGAAGAAAACAGGGATTTCTGATGTGCATCCTATTCTAAAGCCATTGGATGGCGACATACCAAAAGAACAAACAATCAATGGCTCAGATAATGCCAATGAGCAAGTTCCCTCATTAACTATAGTTGACAGTGACAGACTGCTTTTCTCTGTTTTCTCATTACTTCAAAAGCTTGGAGCCGATGAGAGGCCAGAGGTTAGAAATTCAGCGGTCAGGACATTGTTTCAGACACTTGGAAGTCACGGGCAAAAACTTTCGAAAAGCATGTGGGAAGATTGCCTTTGGAATTATGTGTTTCCTACACTCGATCGTGCTTCTCACATGGCTGCAACTTCATCGAAAGATGAATGGCATGGGAAAGAACTTGGAACTCGAGGAGGAAAAGCAGTTCATATGCTCATACATCACAGTCGCAACACAGCTCAGAAACAGTGGGATGAGACGTTAGTACTTGTCCTTGGTGGCGTAGCCCGTATTCTACGCTCATTCTTTCCTTTTCTTAGAAGTTTAAGCAATTTTTGGTCAGGATGGGAGTCTTTGCTTTTATTTGTGAAGAATAGTGTATTAAATGGAAGCAAAGAGGTTGCGCTTGCTGCAATAAATTGCTTACAGACACCTATTCTTTCTCATTCTTCCAAGGGGAACTTGCCGATGCCTTATCTTGAATCAGTACTTGATGTTTACGAGCTTGTTCTTCAAAATTCAACAAACCTGAGTGGAAATGCAGCCAGCAAGGTGAAGCAGGAGATTTTGCAGAGTCTTGGGGAACTATATGTGCAAGCACAAAGGATGTTTGATGATCGGTTGTATACACAGTTGCTTGCAGTCATACGCTTGGCTATGAAAGATGCCATAGTTGCCAACGATAACTGTGAAACTGAATATGGAAATGTTCCACCTGTACTACGTACTGTGCTCGAGATCATTCCAATGTTATGTCCAACAGAGCACATTTCTTCAATGTGGCTTATTCTTCTTCGGGATTTTTCTCAATTTCTGCCAAGATTGAATTCTACCGTACAAATTGAGGAAGATGAAGCAGAGGAAGCTAGCACTAGTGACCGAGTTCCAGATACTCACTTGAAAATAAAACATGAAAGACCTAATGGGACGGCTTCGATGACTCCAGGAGTAGGAGATTCTCCTAGTTCTAGATTAAAGACATCCGTAACAGCCAGCATTCCTAATTATATGTTTGCGGAAAAGCTTGTTCCCTTGTTGGTAGATCTTTTCCTACGGGCTCCAGTAGTTGAAAAGTATATCTTGTATCCTGAAATTATTCAAAGTCTTGGGAGGTGTATGACTACAAGAAGAGACAATCCAGATGGTTCTCTTTGGAGATTAGCTGTCGAAGGCTTCAATCGCATTCTTGTTGATGATGCCAGCAACTCCACTGTAAATGCTGGATCAGATTCAGGTGCTAGTAAGCCTGCAAGAACACGTATATGGAAGGAAGTTGCCGATGTTTATGAAGTATTCCTTGTGGGTTATTGTGGCCGAGCTCTTCCTTCTGATTCCTTCTCAACAGTGGATGTAAAAGCTGACGAGTCCCTTGAGATGACTATCTTGCACATTCTTGGCGACAAGATTTTGAAGTCACCAATTGATGCACCTACCGATATTTTGCAGCGTCTCGTTTCCACTTTGGACCGTTGTGCTTCACGTACTGGTTCTTTACCTGTTGATTTTGTGGAACGTATGCCTTCCCACTGCAGCAGGTTTTCCTTGATTTGCTTACAGAAGTTGTTTTCCTTGAGCAGTTATGACACCAAAGACCATGATTGGAATTCAGCGAGAGCTGAGGTGAGTAAAATTGCAATCATGATACTGATGATGAGATGTGAATACATCTTAAGCAGATTTCTGAATGACGAAAATGATTTAGGTGGACGCCCATTGCCAGCAGCAAGACTTGAAGAAATCATATATGTTCTTGAAGAGTTGGGCCGACTAGTAATTCATTCAGATACTGCATCCATTCTTCCCTTGCAACCATATTTGAAAGGTGCTCTATCAAGAGACAAGAATCACGATGACCGTTCGCACCTTCTTGTTCTATTTCCATCCTTTTCTGAGCTCATAGTGTCAAGAGAAGCAAAAGTAAGAGCATTGGTTCAAGTCTTGTTTAGACTCATCAGCAAAGAATTGTCACTTGAAAGAGTTTCCCTTACCAGTCAAGCTGTGGAAGAATCTCAAAGGATACCGCAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7_N

Protein Analysis

1668

Amino Acids

183.58

Weight (kDa)

5.68

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 3 - 169 1.3e-38 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 229 - 388 3.4e-16 Mon2/Sec7/BIG1-like, HUS domain
Sec7-like_HDS PF09324 806 - 872 8.5e-07 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 877 - 1164 5.9e-49 C-terminal region of Mon2 protein
Mon2_C PF16206 1327 - 1584 1.1e-14 C-terminal region of Mon2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 101
AarI CACCTGC 2 cut(s) 707, 1465
AasI GACNNNNNNGTC 1 cut(s) 216
Acc16I TGCGCA 2 cut(s) 138, 449
Acc36I ACCTGC 6 cut(s) 707, 838, 1465, 2128, 2656, 4500
AccB7I CCANNNNNTGG 2 cut(s) 575, 2843
AccI GTMKAC 2 cut(s) 220, 3602
AccII CGCG 2 cut(s) 36, 1893
AccIII TCCGGA 1 cut(s) 1418
AclI AACGTT 1 cut(s) 1161
AcoI YGGCCR 3 cut(s) 2227, 2534, 4288
AcsI RAATTY 8 cut(s) 1670, 2002, 2251, 2484, 2995, 3493, 3802, 4579
AcuI CTGAAG 2 cut(s) 1607, 2559
AcyI GRCGYC 1 cut(s) 4684
AdeI CACNNNGTG 3 cut(s) 1562, 1840, 2200
AfiI CCNNNNNNNGG 5 cut(s) 575, 1855, 2843, 2979, 3926
AflII CTTAAG 3 cut(s) 323, 1801, 4645
AflIII ACRYGT 2 cut(s) 739, 4232
AhdI GACNNNNNGTC 1 cut(s) 944
AhlI ACTAGT 3 cut(s) 1223, 3848, 4744
AjnI CCWGG 7 cut(s) 124, 1608, 1726, 1794, 1853, 2785, 3919
AjuI GAANNNNNNNTTGG 2 cut(s) 3787, 3819
AleI CACNNNNGTG 2 cut(s) 1937, 2407
AloI GAACNNNNNNTCC 4 cut(s) 820, 852, 3165, 3197
Alw21I GWGCWC 9 cut(s) 93, 2024, 2068, 2698, 3711, 3744, 4297, 4804, 4872
Alw26I GTCTC 8 cut(s) 509, 647, 1214, 1496, 3227, 4101, 4430, 4806
Alw44I GTGCAC 1 cut(s) 2064
AlwNI CAGNNNCTG 3 cut(s) 853, 2611, 4763
Ama87I CYCGRG 2 cut(s) 3165, 3710
Aor13HI TCCGGA 1 cut(s) 1418
ApaI GGGCCC 1 cut(s) 1850
ApaLI GTGCAC 1 cut(s) 2064
ApoI RAATTY 8 cut(s) 1670, 2002, 2251, 2484, 2995, 3493, 3802, 4579
Asp700I GAANNNNTTC 1 cut(s) 4071
AspLEI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1895, 3382
AsuII TTCGAA 1 cut(s) 3050
AsuNHI GCTAGC 1 cut(s) 3842
AvaI CYCGRG 2 cut(s) 3165, 3710
AvaII GGWCC 7 cut(s) 432, 561, 759, 850, 1731, 2783, 4441
AxyI CCTNAGG 1 cut(s) 1085
BaeGI GKGCMC 2 cut(s) 1850, 2068
BaeI ACNNNNGTAYC 3 cut(s) 2370, 2403, 4985
BalI TGGCCA 2 cut(s) 2229, 2536
BamHI GGATCC 1 cut(s) 781
BanII GRGCYC 5 cut(s) 1850, 2024, 4036, 4297, 4872
BarI GAAGNNNNNNTAC 2 cut(s) 2040, 2072
BauI CACGAG 2 cut(s) 214, 266
BbsI GAAGAC 4 cut(s) 702, 1003, 1372, 1472
Bbv12I GWGCWC 9 cut(s) 93, 2024, 2068, 2698, 3711, 3744, 4297, 4804, 4872
BbvCI CCTCAGC 1 cut(s) 4593
BccI CCATC 8 cut(s) 163, 611, 1858, 2183, 2837, 3315, 4112, 4864
BceAI ACGGC 5 cut(s) 42, 97, 913, 1950, 3921
BcgI CGANNNNNNTGC 2 cut(s) 3111, 3145
BciT130I CCWGG 7 cut(s) 126, 1610, 1728, 1796, 1855, 2787, 3921
BciVI GTATCC 3 cut(s) 2371, 4071, 4986
BclI TGATCA 2 cut(s) 496, 1756
BcoDI GTCTC 8 cut(s) 509, 647, 1214, 1496, 3227, 4101, 4430, 4806
BcuI ACTAGT 3 cut(s) 1223, 3848, 4744
BfoI RGCGCY 1 cut(s) 87
BfrI CTTAAG 3 cut(s) 323, 1801, 4645
BfuAI ACCTGC 6 cut(s) 707, 838, 1465, 2128, 2656, 4500
BfuI GTATCC 3 cut(s) 2371, 4071, 4986
BglI GCCNNNNNGGC 1 cut(s) 1057
BglII AGATCT 2 cut(s) 28, 4018
BlpI GCTNAGC 2 cut(s) 87, 2023
BmcAI AGTACT 2 cut(s) 3243, 3465
Bme1390I CCNGG 7 cut(s) 126, 1610, 1728, 1796, 1855, 2787, 3921
Bme18I GGWCC 7 cut(s) 432, 561, 759, 850, 1731, 2783, 4441
BmeRI GACNNNNNGTC 1 cut(s) 944
BmeT110I CYCGRG 2 cut(s) 3165, 3710
BmiI GGNNCC 8 cut(s) 434, 783, 1289, 1732, 1848, 2205, 2971, 4035
BmrFI CCNGG 7 cut(s) 126, 1610, 1728, 1796, 1855, 2787, 3921
BmrI ACTGGG 2 cut(s) 2078, 2391
BmtI GCTAGC 1 cut(s) 3846
BmuI ACTGGG 2 cut(s) 2078, 2391
BpiI GAAGAC 4 cut(s) 702, 1003, 1372, 1472
BpmI CTGGAG 4 cut(s) 564, 2703, 3903, 4020
Bpu10I CCTNAGC 1 cut(s) 4593
Bpu1102I GCTNAGC 2 cut(s) 87, 2023
Bpu14I TTCGAA 1 cut(s) 3050
BpuEI CTTGAG 6 cut(s) 378, 1097, 2258, 2426, 4367, 4567
BsaAI YACGTR 6 cut(s) 740, 2270, 2468, 3701, 4235, 4457
BsaBI GATNNNNATC 4 cut(s) 1143, 2174, 2820, 4355
BsaHI GRCGYC 1 cut(s) 4684
BsaWI WCCGGW 1 cut(s) 1418
BsaXI ACNNNNNCTCC 2 cut(s) 2195, 2225
Bsc4I CCNNNNNNNGG 5 cut(s) 575, 1855, 2843, 2979, 3926
Bse118I RCCGGY 1 cut(s) 527
Bse21I CCTNAGG 1 cut(s) 1085
Bse3DI GCAATG 4 cut(s) 1112, 1818, 2093, 4688
Bse8I GATNNNNATC 4 cut(s) 1143, 2174, 2820, 4355
BseAI TCCGGA 1 cut(s) 1418
BseBI CCWGG 7 cut(s) 126, 1610, 1728, 1796, 1855, 2787, 3921
BseJI GATNNNNATC 4 cut(s) 1143, 2174, 2820, 4355
BseLI CCNNNNNNNGG 5 cut(s) 575, 1855, 2843, 2979, 3926
BseMI GCAATG 4 cut(s) 1112, 1818, 2093, 4688
BseRI GAGGAG 1 cut(s) 3183
BseSI GKGCMC 2 cut(s) 1850, 2068
BseYI CCCAGC 1 cut(s) 2096
BsgI GTGCAG 1 cut(s) 248
Bsh1236I CGCG 2 cut(s) 36, 1893
Bsh1285I CGRYCG 1 cut(s) 3103
BsiEI CGRYCG 1 cut(s) 3103
BsiHKAI GWGCWC 9 cut(s) 93, 2024, 2068, 2698, 3711, 3744, 4297, 4804, 4872
BsiHKCI CYCGRG 2 cut(s) 3165, 3710
BsiSI CCGG 3 cut(s) 528, 973, 1419
BslFI GGGAC 3 cut(s) 418, 3916, 4327
BslI CCNNNNNNNGG 5 cut(s) 575, 1855, 2843, 2979, 3926
BsmAI GTCTC 8 cut(s) 509, 647, 1214, 1496, 3227, 4101, 4430, 4806
BsmBI CGTCTC 2 cut(s) 3227, 4430
BsmFI GGGAC 3 cut(s) 418, 3916, 4327
BsmI GAATGC 3 cut(s) 1532, 3972, 4158
BsoBI CYCGRG 2 cut(s) 3165, 3710
Bsp119I TTCGAA 1 cut(s) 3050
Bsp120I GGGCCC 1 cut(s) 1846
Bsp13I TCCGGA 1 cut(s) 1418
Bsp1407I TGTACA 2 cut(s) 278, 1207
Bsp1720I GCTNAGC 2 cut(s) 87, 2023
Bsp19I CCATGG 1 cut(s) 2779
BspEI TCCGGA 1 cut(s) 1418
BspFNI CGCG 2 cut(s) 36, 1893
BspHI TCATGA 2 cut(s) 238, 4614
BspLI GGNNCC 8 cut(s) 434, 783, 1289, 1732, 1848, 2205, 2971, 4035
BspMAI CTGCAG 7 cut(s) 336, 463, 718, 1318, 2047, 2653, 4508
BspMI ACCTGC 6 cut(s) 707, 838, 1465, 2128, 2656, 4500
BspOI GCTAGC 1 cut(s) 3846
BspQI GCTCTTC 2 cut(s) 1488, 4302
BspT104I TTCGAA 1 cut(s) 3050
BspTI CTTAAG 3 cut(s) 323, 1801, 4645
BsrDI GCAATG 4 cut(s) 1112, 1818, 2093, 4688
BsrFI RCCGGY 1 cut(s) 527
BsrGI TGTACA 2 cut(s) 278, 1207
BssAI RCCGGY 1 cut(s) 527
BssNAI GTATAC 1 cut(s) 3603
BssNI GRCGYC 1 cut(s) 4684
BssSI CACGAG 2 cut(s) 214, 266
BssT1I CCWWGG 4 cut(s) 1350, 2779, 3250, 3429
Bst1107I GTATAC 1 cut(s) 3603
Bst2BI CACGAG 2 cut(s) 214, 266
Bst2UI CCWGG 7 cut(s) 126, 1610, 1728, 1796, 1855, 2787, 3921
Bst6I CTCTTC 8 cut(s) 525, 1224, 1488, 1817, 2578, 4099, 4302, 4725
BstACI GRCGYC 1 cut(s) 4684
BstAFI CTTAAG 3 cut(s) 323, 1801, 4645
BstAPI GCANNNNNTGC 4 cut(s) 853, 1247, 1310, 2693
BstAUI TGTACA 2 cut(s) 278, 1207
BstBAI YACGTR 6 cut(s) 740, 2270, 2468, 3701, 4235, 4457
BstBI TTCGAA 1 cut(s) 3050
BstDSI CCRYGG 2 cut(s) 1932, 2779
BstFNI CGCG 2 cut(s) 36, 1893
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1895, 3382
BstMAI GTCTC 8 cut(s) 509, 647, 1214, 1496, 3227, 4101, 4430, 4806
BstMCI CGRYCG 1 cut(s) 3103
BstNI CCWGG 7 cut(s) 126, 1610, 1728, 1796, 1855, 2787, 3921
BstNSI RCATGY 4 cut(s) 1617, 1913, 2498, 3061
BstSCI CCNGG 7 cut(s) 124, 1608, 1726, 1794, 1853, 2785, 3919
BstSLI GKGCMC 2 cut(s) 1850, 2068
BstSNI TACGTA 2 cut(s) 2270, 3701
BstUI CGCG 2 cut(s) 36, 1893
BstV2I GAAGAC 4 cut(s) 702, 1003, 1372, 1472
BstX2I RGATCY 8 cut(s) 28, 620, 722, 781, 1080, 2111, 2474, 4018
BstXI CCANNNNNNTGG 4 cut(s) 792, 814, 1165, 2786
BstYI RGATCY 8 cut(s) 28, 620, 722, 781, 1080, 2111, 2474, 4018
BstZ17I GTATAC 1 cut(s) 3603
Bsu36I CCTNAGG 1 cut(s) 1085
BsuI GTATCC 3 cut(s) 2371, 4071, 4986
BtgI CCRYGG 2 cut(s) 1932, 2779
BtsI GCAGTG 1 cut(s) 4501
BveI ACCTGC 6 cut(s) 707, 838, 1465, 2128, 2656, 4500
CaiI CAGNNNCTG 3 cut(s) 853, 2611, 4763
CciI TCATGA 2 cut(s) 238, 4614
CfoI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1895, 3382
Cfr10I RCCGGY 1 cut(s) 527
CseI GACGC 3 cut(s) 948, 4412, 4692
DraIII CACNNNGTG 3 cut(s) 1562, 1840, 2200
DrdI GACNNNNNNGTC 1 cut(s) 216
DriI GACNNNNNGTC 1 cut(s) 944
DseDI GACNNNNNNGTC 1 cut(s) 216
EaeI YGGCCR 3 cut(s) 2227, 2534, 4288
Eam1104I CTCTTC 8 cut(s) 525, 1224, 1488, 1817, 2578, 4099, 4302, 4725
Eam1105I GACNNNNNGTC 1 cut(s) 944
EarI CTCTTC 8 cut(s) 525, 1224, 1488, 1817, 2578, 4099, 4302, 4725
EciI GGCGGA 2 cut(s) 34, 2807
Ecl136II GAGCTC 3 cut(s) 2022, 4295, 4870
Eco105I TACGTA 2 cut(s) 2270, 3701
Eco130I CCWWGG 4 cut(s) 1350, 2779, 3250, 3429
Eco147I AGGCCT 3 cut(s) 1601, 1799, 2736
Eco24I GRGCYC 5 cut(s) 1850, 2024, 4036, 4297, 4872
Eco32I GATATC 2 cut(s) 1595, 2172
Eco47I GGWCC 7 cut(s) 432, 561, 759, 850, 1731, 2783, 4441
Eco53kI GAGCTC 3 cut(s) 2022, 4295, 4870
Eco57I CTGAAG 2 cut(s) 1607, 2559
Eco81I CCTNAGG 1 cut(s) 1085
Eco88I CYCGRG 2 cut(s) 3165, 3710
EcoICRI GAGCTC 3 cut(s) 2022, 4295, 4870
EcoO109I RGGNCCY 2 cut(s) 432, 850
EcoRI GAATTC 3 cut(s) 2002, 3802, 4579
EcoRII CCWGG 7 cut(s) 124, 1608, 1726, 1794, 1853, 2785, 3919
EcoRV GATATC 2 cut(s) 1595, 2172
EcoT14I CCWWGG 4 cut(s) 1350, 2779, 3250, 3429
EcoT22I ATGCAT 4 cut(s) 1306, 1534, 2093, 2290
EcoT38I GRGCYC 5 cut(s) 1850, 2024, 4036, 4297, 4872
ErhI CCWWGG 4 cut(s) 1350, 2779, 3250, 3429
Esp3I CGTCTC 2 cut(s) 3227, 4430
FalI AAGNNNNNCTT 8 cut(s) 3057, 3089, 3422, 3454, 4040, 4072, 4257, 4289
FaqI GGGAC 3 cut(s) 418, 3916, 4327
FauI CCCGC 1 cut(s) 2607
FauNDI CATATG 1 cut(s) 3186
FbaI TGATCA 2 cut(s) 496, 1756
FblI GTMKAC 2 cut(s) 220, 3602
FriOI GRGCYC 5 cut(s) 1850, 2024, 4036, 4297, 4872
FspI TGCGCA 2 cut(s) 138, 449
GlaI GCGC 7 cut(s) 37, 85, 138, 449, 511, 1894, 3381
GsaI CCCAGC 1 cut(s) 2100
GsuI CTGGAG 4 cut(s) 564, 2703, 3903, 4020
HaeII RGCGCY 1 cut(s) 87
HapII CCGG 3 cut(s) 528, 973, 1419
HgaI GACGC 3 cut(s) 948, 4412, 4692
HhaI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1895, 3382
Hin1I GRCGYC 1 cut(s) 4684
Hin6I GCGC 7 cut(s) 36, 84, 137, 448, 510, 1893, 3380
HinP1I GCGC 7 cut(s) 36, 84, 137, 448, 510, 1893, 3380
HincII GTYRAC 3 cut(s) 1518, 2461, 2920
HindII GTYRAC 3 cut(s) 1518, 2461, 2920
HindIII AAGCTT 5 cut(s) 227, 533, 674, 2963, 3998
HpaII CCGG 3 cut(s) 528, 973, 1419
Hpy99I CGWCG 2 cut(s) 63, 942
HpyCH4IV ACGT 9 cut(s) 739, 1161, 2269, 2467, 3236, 3700, 4234, 4456, 4489
HpySE526I ACGT 9 cut(s) 739, 1161, 2269, 2467, 3236, 3700, 4234, 4456, 4489
Hsp92I GRCGYC 1 cut(s) 4684
HspAI GCGC 7 cut(s) 36, 84, 137, 448, 510, 1893, 3380
Kpn2I TCCGGA 1 cut(s) 1418
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Ksp22I TGATCA 2 cut(s) 496, 1756
LguI GCTCTTC 2 cut(s) 1488, 4302
LmnI GCTCC 8 cut(s) 111, 904, 1287, 1707, 2203, 2684, 2969, 4039
MaeII ACGT 9 cut(s) 739, 1161, 2269, 2467, 3236, 3700, 4234, 4456, 4489
MfeI CAATTG 1 cut(s) 4395
MflI RGATCY 8 cut(s) 28, 620, 722, 781, 1080, 2111, 2474, 4018
MlsI TGGCCA 2 cut(s) 2229, 2536
MluNI TGGCCA 2 cut(s) 2229, 2536
MmeI TCCRAC 4 cut(s) 831, 1557, 2119, 3758
Mox20I TGGCCA 2 cut(s) 2229, 2536
Mph1103I ATGCAT 4 cut(s) 1306, 1534, 2093, 2290
MroI TCCGGA 1 cut(s) 1418
MroNI GCCGGC 1 cut(s) 527
MroXI GAANNNNTTC 1 cut(s) 4071
MscI TGGCCA 2 cut(s) 2229, 2536
MslI CAYNNNNRTG 5 cut(s) 1937, 2086, 2407, 3722, 4237
Msp20I TGGCCA 2 cut(s) 2229, 2536
MspA1I CMGCKG 4 cut(s) 757, 1244, 2011, 3002
MspCI CTTAAG 3 cut(s) 323, 1801, 4645
MspI CCGG 3 cut(s) 528, 973, 1419
MspR9I CCNGG 7 cut(s) 126, 1610, 1728, 1796, 1855, 2787, 3921
MunI CAATTG 1 cut(s) 4395
Mva1269I GAATGC 3 cut(s) 1532, 3972, 4158
MvaI CCWGG 7 cut(s) 126, 1610, 1728, 1796, 1855, 2787, 3921
MvnI CGCG 2 cut(s) 36, 1893
NaeI GCCGGC 1 cut(s) 529
NcoI CCATGG 1 cut(s) 2779
NdeI CATATG 1 cut(s) 3186
NgoMIV GCCGGC 1 cut(s) 527
NheI GCTAGC 1 cut(s) 3842
NlaIV GGNNCC 8 cut(s) 434, 783, 1289, 1732, 1848, 2205, 2971, 4035
NmeAIII GCCGAG 2 cut(s) 73, 4316
NsbI TGCGCA 2 cut(s) 138, 449
NsiI ATGCAT 4 cut(s) 1306, 1534, 2093, 2290
NspI RCATGY 4 cut(s) 1617, 1913, 2498, 3061
NspV TTCGAA 1 cut(s) 3050
OliI CACNNNNGTG 2 cut(s) 1937, 2407
PaeI GCATGC 2 cut(s) 1617, 1913
PaeR7I CTCGAG 2 cut(s) 3165, 3710
PagI TCATGA 2 cut(s) 238, 4614
PaqCI CACCTGC 2 cut(s) 707, 1465
PceI AGGCCT 3 cut(s) 1601, 1799, 2736
PciSI GCTCTTC 2 cut(s) 1488, 4302
PctI GAATGC 3 cut(s) 1532, 3972, 4158
PdiI GCCGGC 1 cut(s) 529
PdmI GAANNNNTTC 1 cut(s) 4071
PflMI CCANNNNNTGG 2 cut(s) 575, 2843
PfoI TCCNGGA 1 cut(s) 3919
Ple19I CGATCG 1 cut(s) 3103
Ppu21I YACGTR 6 cut(s) 740, 2270, 2468, 3701, 4235, 4457
PpuMI RGGWCCY 2 cut(s) 432, 850
PsiI TTATAA 1 cut(s) 101
Psp124BI GAGCTC 3 cut(s) 2024, 4297, 4872
Psp1406I AACGTT 1 cut(s) 1161
Psp5II RGGWCCY 2 cut(s) 432, 850
Psp6I CCWGG 7 cut(s) 124, 1608, 1726, 1794, 1853, 2785, 3919
PspFI CCCAGC 1 cut(s) 2096
PspGI CCWGG 7 cut(s) 124, 1608, 1726, 1794, 1853, 2785, 3919
PspN4I GGNNCC 8 cut(s) 434, 783, 1289, 1732, 1848, 2205, 2971, 4035
PspOMI GGGCCC 1 cut(s) 1846
PspPPI RGGWCCY 2 cut(s) 432, 850
PspXI VCTCGAGB 1 cut(s) 3165
PstI CTGCAG 7 cut(s) 336, 463, 718, 1318, 2047, 2653, 4508
PstNI CAGNNNCTG 3 cut(s) 853, 2611, 4763
PsuI RGATCY 8 cut(s) 28, 620, 722, 781, 1080, 2111, 2474, 4018
PvuI CGATCG 1 cut(s) 3103
PvuII CAGCTG 2 cut(s) 1244, 2011
RseI CAYNNNNRTG 5 cut(s) 1937, 2086, 2407, 3722, 4237
SacI GAGCTC 3 cut(s) 2024, 4297, 4872
SapI GCTCTTC 2 cut(s) 1488, 4302
ScaI AGTACT 2 cut(s) 3243, 3465
ScrFI CCNGG 7 cut(s) 126, 1610, 1728, 1796, 1855, 2787, 3921
Sfr274I CTCGAG 2 cut(s) 3165, 3710
SfuI TTCGAA 1 cut(s) 3050
SinI GGWCC 7 cut(s) 432, 561, 759, 850, 1731, 2783, 4441
SlaI CTCGAG 2 cut(s) 3165, 3710
SmiMI CAYNNNNRTG 5 cut(s) 1937, 2086, 2407, 3722, 4237
SnaBI TACGTA 2 cut(s) 2270, 3701
SpeI ACTAGT 3 cut(s) 1223, 3848, 4744
SphI GCATGC 2 cut(s) 1617, 1913
SseBI AGGCCT 3 cut(s) 1601, 1799, 2736
SspI AATATT 3 cut(s) 351, 1987, 2542
SstI GAGCTC 3 cut(s) 2024, 4297, 4872
StuI AGGCCT 3 cut(s) 1601, 1799, 2736
StyD4I CCNGG 7 cut(s) 124, 1608, 1726, 1794, 1853, 2785, 3919
StyI CCWWGG 4 cut(s) 1350, 2779, 3250, 3429
TaiI ACGT 9 cut(s) 742, 1164, 2272, 2470, 3239, 3703, 4237, 4459, 4492
TaqII GACCGA 2 cut(s) 320, 3870
TatI WGTACW 5 cut(s) 278, 1207, 3241, 3463, 3694
TspGWI ACGGA 4 cut(s) 98, 1415, 2646, 3949
Van91I CCANNNNNTGG 2 cut(s) 575, 2843
Vha464I CTTAAG 3 cut(s) 323, 1801, 4645
VneI GTGCAC 1 cut(s) 2064
VpaK11BI GGWCC 7 cut(s) 432, 561, 759, 850, 1731, 2783, 4441
XapI RAATTY 8 cut(s) 1670, 2002, 2251, 2484, 2995, 3493, 3802, 4579
XbaI TCTAGA 1 cut(s) 3944
XceI RCATGY 4 cut(s) 1617, 1913, 2498, 3061
XcmI CCANNNNNNNNNTGG 1 cut(s) 4972
XhoI CTCGAG 2 cut(s) 3165, 3710
XmiI GTMKAC 2 cut(s) 220, 3602
XmnI GAANNNNTTC 1 cut(s) 4071
ZrmI AGTACT 2 cut(s) 3243, 3465
Zsp2I ATGCAT 4 cut(s) 1306, 1534, 2093, 2290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.