RchiOBHm_Chr3g0474721

Belongs to the ATG8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0019128
Physical Location & Seq
Reverse (-)
20445090 .. 20445560
471 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGTTGAGGCAAGCCAGTGAAACTTTCATCAGCAGCAATGGCCATACCAGGGGGGACTTCAAGCGCCGACATTCTCTAGGGCCAAGGCGAGCCAAAGTATTGCGTATCAGGGAGAAGTATCCTGATAGAATACCTGTGATTGTGGAGAAAGCTATATTCACCAACATTCCTAACATTGCCAAGGACAAATTCCTTATCCCTCCCGATATTGCTCTTGGACAATTTGCTTATGTTATTCAAAAGATGATCCACCTCAAAGCGGACAAGGCTATATTTGTCTTCGTCAACAACACTTTACCTCGCACTTCTGCCTTGATGTCTGAAATTTATGCAAAAAAGCGGATGAAGATGGATTCCTTTACGTGGTATACAGTGGAGAGGGTGCCTTCGGGTCACTTTAAGAAGAGCTTATATAGGATCCAGGTAATCTTAAGAGTGTGGTTTTGGTTAGGACTTTACAAATGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

156

Amino Acids

18.39

Weight (kDa)

10.56

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 29 - 122 1.8e-30 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019128)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0474721
rosa_multiflora Rmu_co8405031.1_g000001
rosa_roxburghii Rroxscaffold_6G00406940
rosa_rugosa Rorug03G0142900
rosa_samantha Rh3AG193200 Rh3BG223000 Rh3CG217800 Rh3DG219000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AccI GTMKAC 1 cut(s) 368
AciI CCGC 2 cut(s) 260, 340
AclWI GGATC 3 cut(s) 241, 412, 425
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 2 cut(s) 188, 324
AfiI CCNNNNNNNGG 3 cut(s) 49, 259, 363
AflII CTTAAG 1 cut(s) 430
AgsI TTSAA 2 cut(s) 61, 239
AjnI CCWGG 2 cut(s) 47, 420
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 2 cut(s) 152, 408
AluI AGCT 2 cut(s) 152, 408
AlwI GGATC 3 cut(s) 241, 412, 425
AoxI GGCC 2 cut(s) 40, 80
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 2 cut(s) 188, 324
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 151
BalI TGGCCA 1 cut(s) 42
BamHI GGATCC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 45
BccI CCATC 1 cut(s) 343
BciT130I CCWGG 2 cut(s) 49, 422
BciVI GTATCC 1 cut(s) 129
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 1 cut(s) 67
BfrI CTTAAG 1 cut(s) 430
BfuI GTATCC 1 cut(s) 129
BisI GCNGC 1 cut(s) 34
BlsI GCNGC 1 cut(s) 35
Bme1390I CCNGG 2 cut(s) 49, 422
BmgT120I GGNCC 1 cut(s) 80
BmiI GGNNCC 2 cut(s) 384, 419
BmrFI CCNGG 2 cut(s) 49, 422
BpiI GAAGAC 1 cut(s) 271
BsaAI YACGTR 1 cut(s) 363
BsaJI CCNNGG 3 cut(s) 48, 83, 180
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 259, 363
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 2 cut(s) 43, 174
BseBI CCWGG 2 cut(s) 49, 422
BseDI CCNNGG 3 cut(s) 48, 83, 180
BseGI GGATG 1 cut(s) 348
BseLI CCNNNNNNNGG 3 cut(s) 49, 259, 363
BseMI GCAATG 2 cut(s) 43, 174
BseNI ACTGG 1 cut(s) 15
BseXI GCAGC 1 cut(s) 45
BshFI GGCC 2 cut(s) 42, 82
BshNI GGYRCC 1 cut(s) 382
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 3 cut(s) 49, 259, 363
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 2 cut(s) 42, 82
Bsp143I GATC 2 cut(s) 246, 417
BspACI CCGC 2 cut(s) 260, 340
BspANI GGCC 2 cut(s) 42, 82
BspLI GGNNCC 2 cut(s) 384, 419
BspPI GGATC 3 cut(s) 241, 412, 425
BspQI GCTCTTC 1 cut(s) 398
BspT107I GGYRCC 1 cut(s) 382
BspTI CTTAAG 1 cut(s) 430
BsrDI GCAATG 2 cut(s) 43, 174
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 3 cut(s) 48, 83, 180
BssMI GATC 2 cut(s) 246, 417
BssNAI GTATAC 1 cut(s) 369
BssT1I CCWWGG 2 cut(s) 83, 180
Bst1107I GTATAC 1 cut(s) 369
Bst2UI CCWGG 2 cut(s) 49, 422
Bst4CI ACNGT 1 cut(s) 373
Bst6I CTCTTC 1 cut(s) 398
BstAFI CTTAAG 1 cut(s) 430
BstBAI YACGTR 1 cut(s) 363
BstC8I GCNNGC 2 cut(s) 12, 90
BstF5I GGATG 1 cut(s) 348
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 2 cut(s) 249, 420
BstMBI GATC 2 cut(s) 246, 417
BstMWI GCNNNNNNNGC 2 cut(s) 39, 266
BstNI CCWGG 2 cut(s) 49, 422
BstSCI CCNGG 2 cut(s) 47, 420
BstV1I GCAGC 1 cut(s) 45
BstV2I GAAGAC 1 cut(s) 271
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
BstZ17I GTATAC 1 cut(s) 369
BsuI GTATCC 1 cut(s) 129
BsuRI GGCC 2 cut(s) 42, 82
BtsCI GGATG 1 cut(s) 348
BtsIMutI CAGTG 2 cut(s) 22, 378
Cac8I GCNNGC 2 cut(s) 12, 90
CfoI GCGC 1 cut(s) 66
Cfr13I GGNCC 1 cut(s) 80
CviJI RGCY 7 cut(s) 14, 42, 82, 92, 152, 269, 408
CviKI_1 RGCY 7 cut(s) 14, 42, 82, 92, 152, 269, 408
DpnI GATC 2 cut(s) 248, 419
DpnII GATC 2 cut(s) 246, 417
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
Eco130I CCWWGG 2 cut(s) 83, 180
EcoRII CCWGG 2 cut(s) 47, 420
EcoT14I CCWWGG 2 cut(s) 83, 180
ErhI CCWWGG 2 cut(s) 83, 180
FaiI YATR 8 cut(s) 45, 155, 231, 272, 330, 369, 412, 414
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FaqI GGGAC 1 cut(s) 68
FblI GTMKAC 1 cut(s) 368
Fnu4HI GCNGC 1 cut(s) 34
FokI GGATG 1 cut(s) 355
Fsp4HI GCNGC 1 cut(s) 34
FspBI CTAG 1 cut(s) 77
GlaI GCGC 1 cut(s) 65
GluI GCNGC 1 cut(s) 34
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 2 cut(s) 42, 82
HhaI GCGC 1 cut(s) 66
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 1 cut(s) 353
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 2 cut(s) 286, 369
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 2 cut(s) 122, 203
Hpy8I GTNNAC 2 cut(s) 286, 369
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 1 cut(s) 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 266
HpySE526I ACGT 1 cut(s) 362
HspAI GCGC 1 cut(s) 64
Kzo9I GATC 2 cut(s) 246, 417
LguI GCTCTTC 1 cut(s) 398
LpnPI CCDG 8 cut(s) 28, 34, 61, 94, 135, 147, 407, 434
Lsp1109I GCAGC 1 cut(s) 45
MaeI CTAG 1 cut(s) 77
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 1 cut(s) 392
MalI GATC 2 cut(s) 248, 419
MboI GATC 2 cut(s) 246, 417
MboII GAAGA 3 cut(s) 271, 358, 415
MflI RGATCY 1 cut(s) 417
MlsI TGGCCA 1 cut(s) 42
MluCI AATT 3 cut(s) 188, 221, 324
MluNI TGGCCA 1 cut(s) 42
MnlI CCTC 4 cut(s) 210, 263, 309, 372
Mox20I TGGCCA 1 cut(s) 42
MscI TGGCCA 1 cut(s) 42
MseI TTAA 2 cut(s) 399, 431
Msp20I TGGCCA 1 cut(s) 42
MspCI CTTAAG 1 cut(s) 430
MspR9I CCNGG 2 cut(s) 49, 422
MvaI CCWGG 2 cut(s) 49, 422
MwoI GCNNNNNNNGC 2 cut(s) 39, 266
NdeII GATC 2 cut(s) 246, 417
NlaIV GGNNCC 2 cut(s) 384, 419
NmuCI GTSAC 1 cut(s) 392
PciSI GCTCTTC 1 cut(s) 398
PfeI GAWTC 1 cut(s) 353
PkrI GCNGC 1 cut(s) 35
Ppu21I YACGTR 1 cut(s) 363
Psp6I CCWGG 2 cut(s) 47, 420
PspGI CCWGG 2 cut(s) 47, 420
PspN4I GGNNCC 2 cut(s) 384, 419
PspPI GGNCC 1 cut(s) 80
PsuI RGATCY 1 cut(s) 417
SapI GCTCTTC 1 cut(s) 398
SaqAI TTAA 2 cut(s) 399, 431
SatI GCNGC 1 cut(s) 34
Sau3AI GATC 2 cut(s) 246, 417
Sau96I GGNCC 1 cut(s) 80
ScrFI CCNGG 2 cut(s) 49, 422
SetI ASST 7 cut(s) 136, 154, 255, 301, 365, 410, 426
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
Sse9I AATT 3 cut(s) 188, 221, 324
SsiI CCGC 2 cut(s) 260, 340
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 2 cut(s) 47, 420
StyI CCWWGG 2 cut(s) 83, 180
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 1 cut(s) 365
TasI AATT 3 cut(s) 188, 221, 324
TfiI GAWTC 1 cut(s) 353
Tru1I TTAA 2 cut(s) 399, 431
Tru9I TTAA 2 cut(s) 399, 431
TscAI CASTG 2 cut(s) 22, 378
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 2 cut(s) 16, 359
TspRI CASTG 2 cut(s) 22, 378
Vha464I CTTAAG 1 cut(s) 430
XapI RAATTY 2 cut(s) 188, 324
XmiI GTMKAC 1 cut(s) 368
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.