Rroxscaffold_6G00406940

Ubiquitin-like autophagy protein Apg12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0019128
Physical Location & Seq
Forward (+)
29477673 .. 29477948
276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00406940.1

Sequence Viewer

Length: 276 bp
ATGGCCATACCGGGGGGACTTCAAGCGCCGACATTCTCTAGGGCCGAGGCGAGCCGAAGTAGTGCGTATCGGGAGAAGTATCCTGATAGAATACCCGTGATTGTGGAGAAAGCTATATGCACCGACATTCCTAACATTGCCAAGAACAAATTCCTTATCCCTCCCGATATTGCTCTTGGACAATTTGCTTATGTTATTCCAAAGATGATCCACCTCAAAGCGGACAAGGCAATATTTGTCTTCGTCAACAACACTTTACCTCGCACTTCGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

91

Amino Acids

10.05

Weight (kDa)

9.66

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 20 - 90 1.8e-24 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019128)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0474721
rosa_multiflora Rmu_co8405031.1_g000001
rosa_roxburghii Rroxscaffold_6G00406940
rosa_rugosa Rorug03G0142900
rosa_samantha Rh3AG193200 Rh3BG223000 Rh3CG217800 Rh3DG219000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 221
AclWI GGATC 1 cut(s) 202
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 12, 220
AgsI TTSAA 1 cut(s) 23
AjuI GAANNNNNNNTTGG 2 cut(s) 134, 166
AluBI AGCT 1 cut(s) 113
AluI AGCT 1 cut(s) 113
AlwI GGATC 1 cut(s) 202
AoxI GGCC 2 cut(s) 3, 42
ApoI RAATTY 1 cut(s) 149
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 42
AsuC2I CCSGG 1 cut(s) 12
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 232
BciVI GTATCC 1 cut(s) 90
BcnI CCSGG 1 cut(s) 12
BfaI CTAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 29
BfuI GTATCC 1 cut(s) 90
Bme1390I CCNGG 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 42
BmrFI CCNGG 1 cut(s) 12
BpiI GAAGAC 1 cut(s) 232
BpuMI CCSGG 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 11, 45
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 220
Bse3DI GCAATG 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 11, 45
BseLI CCNNNNNNNGG 2 cut(s) 12, 220
BseMI GCAATG 1 cut(s) 135
BshFI GGCC 2 cut(s) 5, 44
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 2 cut(s) 12, 220
BsmFI GGGAC 1 cut(s) 30
BsnI GGCC 2 cut(s) 5, 44
Bsp143I GATC 1 cut(s) 207
BspACI CCGC 1 cut(s) 221
BspANI GGCC 2 cut(s) 5, 44
BspPI GGATC 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 135
BssECI CCNNGG 2 cut(s) 11, 45
BssMI GATC 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 52
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 1 cut(s) 210
BstMBI GATC 1 cut(s) 207
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 10
BstV2I GAAGAC 1 cut(s) 232
BsuI GTATCC 1 cut(s) 90
BsuRI GGCC 2 cut(s) 5, 44
Cac8I GCNNGC 1 cut(s) 52
CfoI GCGC 1 cut(s) 28
Cfr13I GGNCC 1 cut(s) 42
CviJI RGCY 4 cut(s) 5, 44, 54, 113
CviKI_1 RGCY 4 cut(s) 5, 44, 54, 113
DpnI GATC 1 cut(s) 209
DpnII GATC 1 cut(s) 207
EaeI YGGCCR 1 cut(s) 3
FaiI YATR 4 cut(s) 8, 116, 118, 192
FaqI GGGAC 1 cut(s) 30
FspBI CTAG 1 cut(s) 39
GlaI GCGC 1 cut(s) 27
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 2 cut(s) 5, 44
HapII CCGG 1 cut(s) 11
HhaI GCGC 1 cut(s) 28
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HpaII CCGG 1 cut(s) 11
Hpy166II GTNNAC 1 cut(s) 247
Hpy188III TCNNGA 3 cut(s) 71, 83, 164
Hpy8I GTNNAC 1 cut(s) 247
HpyCH4V TGCA 1 cut(s) 120
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HspAI GCGC 1 cut(s) 26
Kzo9I GATC 1 cut(s) 207
LpnPI CCDG 2 cut(s) 24, 96
MaeI CTAG 1 cut(s) 39
MalI GATC 1 cut(s) 209
MboI GATC 1 cut(s) 207
MboII GAAGA 1 cut(s) 232
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 149, 182
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 4 cut(s) 40, 171, 224, 270
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 149
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 227
NciI CCSGG 1 cut(s) 12
NdeII GATC 1 cut(s) 207
NmeAIII GCCGAG 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 149
PspPI GGNCC 1 cut(s) 42
Sau3AI GATC 1 cut(s) 207
Sau96I GGNCC 1 cut(s) 42
ScrFI CCNGG 1 cut(s) 12
SetI ASST 3 cut(s) 115, 216, 262
Sse9I AATT 2 cut(s) 149, 182
SsiI CCGC 1 cut(s) 221
SspI AATATT 1 cut(s) 234
SspMI CTAG 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 10
TasI AATT 2 cut(s) 149, 182
XapI RAATTY 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.