Rh3BG223000

Belongs to the ATG8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0019128
Physical Location & Seq
Reverse (-)
20276703 .. 20283166
6464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG223000.1

Sequence Viewer

Length: 591 bp
ATGTTGAGGCAAGCCAGTGAAACTTTCATCAGCAGCAATGGCCATACCAGGGGGGACTTCAAGCGCCGACATTCTCTAGGGCCAAGGCGAGCCAAAGTATTGCGTATCAGGGAGAAGTATCCTGATAGAATACCTGTGATTGTGGAGAAAGCTATATTCACCAACATTCCTAACATTGCCAAGGACAAATTCCTTATCCCTCCCGATATTGCTCTTGGACAATTTGCTTATGTTATTCAAAAGATGATCCACCTCAAAGCGGACAAGGCTATATTTGTCTTCGTCAACAACACTTTACCTCGCACTTCTGCCTTGATGTCTGAAATTTATGCAAAAAAGCGGATGAAGATGGATTCCTTTACGTGGTATACAGTGGAGAGGGTGCCTTCGGGTCACTTTAAGAAGAGCTTATATAGGATCCAGTTCGTCCAAATCCTAGAGATTCAGGGCTTTGCTTTATACTGCCCACAAGACAGTGTAGCGGCCCAAAGCATTAACTCCACGTATTCTTATAGAGATGGCATAGACGTCAAACAAGAAAGTGAGCAAGGGCAAATCCGTCACCCAGAAAAGCACAGGGCAACTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

196

Amino Acids

22.7

Weight (kDa)

10.0

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 29 - 122 3.3e-30 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019128)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0474721
rosa_multiflora Rmu_co8405031.1_g000001
rosa_roxburghii Rroxscaffold_6G00406940
rosa_rugosa Rorug03G0142900
rosa_samantha Rh3AG193200 Rh3BG223000 Rh3CG217800 Rh3DG219000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 531
AccB1I GGYRCC 1 cut(s) 382
AccI GTMKAC 1 cut(s) 368
AciI CCGC 3 cut(s) 260, 340, 482
AclWI GGATC 3 cut(s) 241, 412, 425
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 2 cut(s) 188, 324
AcyI GRCGYC 1 cut(s) 528
AfiI CCNNNNNNNGG 3 cut(s) 49, 259, 363
AgsI TTSAA 2 cut(s) 61, 239
AjnI CCWGG 1 cut(s) 47
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 2 cut(s) 152, 408
AluI AGCT 2 cut(s) 152, 408
AlwI GGATC 3 cut(s) 241, 412, 425
AoxI GGCC 3 cut(s) 40, 80, 483
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 2 cut(s) 188, 324
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 2 cut(s) 80, 484
AsuHPI GGTGA 2 cut(s) 151, 554
BalI TGGCCA 1 cut(s) 42
BamHI GGATCC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 343, 512
BciT130I CCWGG 1 cut(s) 49
BciVI GTATCC 1 cut(s) 129
BfaI CTAG 2 cut(s) 77, 437
BfoI RGCGCY 1 cut(s) 67
BfuI GTATCC 1 cut(s) 129
BisI GCNGC 2 cut(s) 34, 483
BlsI GCNGC 2 cut(s) 35, 484
Bme1390I CCNGG 1 cut(s) 49
BmgT120I GGNCC 2 cut(s) 80, 484
BmiI GGNNCC 2 cut(s) 384, 419
BmrFI CCNGG 1 cut(s) 49
BpiI GAAGAC 1 cut(s) 271
BsaAI YACGTR 2 cut(s) 363, 504
BsaHI GRCGYC 1 cut(s) 528
BsaJI CCNNGG 3 cut(s) 48, 83, 180
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 259, 363
Bse1I ACTGG 2 cut(s) 15, 421
Bse3DI GCAATG 2 cut(s) 43, 174
BseBI CCWGG 1 cut(s) 49
BseDI CCNNGG 3 cut(s) 48, 83, 180
BseGI GGATG 1 cut(s) 348
BseLI CCNNNNNNNGG 3 cut(s) 49, 259, 363
BseMI GCAATG 2 cut(s) 43, 174
BseNI ACTGG 2 cut(s) 15, 421
BseXI GCAGC 1 cut(s) 45
BshFI GGCC 3 cut(s) 42, 82, 485
BshNI GGYRCC 1 cut(s) 382
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 3 cut(s) 49, 259, 363
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 3 cut(s) 42, 82, 485
Bsp143I GATC 2 cut(s) 246, 417
BspACI CCGC 3 cut(s) 260, 340, 482
BspANI GGCC 3 cut(s) 42, 82, 485
BspLI GGNNCC 2 cut(s) 384, 419
BspPI GGATC 3 cut(s) 241, 412, 425
BspQI GCTCTTC 1 cut(s) 398
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 2 cut(s) 43, 174
BsrI ACTGG 2 cut(s) 15, 421
BssECI CCNNGG 3 cut(s) 48, 83, 180
BssMI GATC 2 cut(s) 246, 417
BssNAI GTATAC 1 cut(s) 369
BssNI GRCGYC 1 cut(s) 528
BssT1I CCWWGG 2 cut(s) 83, 180
Bst1107I GTATAC 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 49
Bst4CI ACNGT 2 cut(s) 373, 476
Bst6I CTCTTC 1 cut(s) 398
BstACI GRCGYC 1 cut(s) 528
BstBAI YACGTR 2 cut(s) 363, 504
BstC8I GCNNGC 2 cut(s) 12, 90
BstF5I GGATG 1 cut(s) 348
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 2 cut(s) 249, 420
BstMBI GATC 2 cut(s) 246, 417
BstMWI GCNNNNNNNGC 2 cut(s) 39, 266
BstNI CCWGG 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 45
BstV2I GAAGAC 1 cut(s) 271
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
BstZ17I GTATAC 1 cut(s) 369
BsuI GTATCC 1 cut(s) 129
BsuRI GGCC 3 cut(s) 42, 82, 485
BtsCI GGATG 1 cut(s) 348
BtsIMutI CAGTG 3 cut(s) 22, 378, 481
Cac8I GCNNGC 2 cut(s) 12, 90
CfoI GCGC 1 cut(s) 66
Cfr13I GGNCC 2 cut(s) 80, 484
CviJI RGCY 9 cut(s) 14, 42, 82, 92, 152, 269, 408, 450, 485
CviKI_1 RGCY 9 cut(s) 14, 42, 82, 92, 152, 269, 408, 450, 485
DpnI GATC 2 cut(s) 248, 419
DpnII GATC 2 cut(s) 246, 417
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
Eco130I CCWWGG 2 cut(s) 83, 180
EcoRII CCWGG 1 cut(s) 47
EcoT14I CCWWGG 2 cut(s) 83, 180
ErhI CCWWGG 2 cut(s) 83, 180
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FaqI GGGAC 1 cut(s) 68
FblI GTMKAC 1 cut(s) 368
Fnu4HI GCNGC 2 cut(s) 34, 483
FokI GGATG 1 cut(s) 355
Fsp4HI GCNGC 2 cut(s) 34, 483
FspBI CTAG 2 cut(s) 77, 437
GlaI GCGC 1 cut(s) 65
GluI GCNGC 2 cut(s) 34, 483
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 3 cut(s) 42, 82, 485
HhaI GCGC 1 cut(s) 66
Hin1I GRCGYC 1 cut(s) 528
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 2 cut(s) 353, 442
HphI GGTGA 2 cut(s) 151, 554
Hpy166II GTNNAC 2 cut(s) 286, 369
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 2 cut(s) 122, 203
Hpy8I GTNNAC 2 cut(s) 286, 369
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 2 cut(s) 373, 476
HpyCH4IV ACGT 3 cut(s) 362, 503, 528
HpyCH4V TGCA 1 cut(s) 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 266
HpySE526I ACGT 3 cut(s) 362, 503, 528
Hsp92I GRCGYC 1 cut(s) 528
HspAI GCGC 1 cut(s) 64
Kzo9I GATC 2 cut(s) 246, 417
LguI GCTCTTC 1 cut(s) 398
Lsp1109I GCAGC 1 cut(s) 45
MaeI CTAG 2 cut(s) 77, 437
MaeII ACGT 3 cut(s) 362, 503, 528
MaeIII GTNAC 2 cut(s) 392, 560
MalI GATC 2 cut(s) 248, 419
MboI GATC 2 cut(s) 246, 417
MboII GAAGA 3 cut(s) 271, 358, 415
MflI RGATCY 1 cut(s) 417
MlsI TGGCCA 1 cut(s) 42
MluCI AATT 3 cut(s) 188, 221, 324
MluNI TGGCCA 1 cut(s) 42
MnlI CCTC 4 cut(s) 210, 263, 309, 372
Mox20I TGGCCA 1 cut(s) 42
MscI TGGCCA 1 cut(s) 42
MseI TTAA 2 cut(s) 399, 495
Msp20I TGGCCA 1 cut(s) 42
MspR9I CCNGG 1 cut(s) 49
MvaI CCWGG 1 cut(s) 49
MwoI GCNNNNNNNGC 2 cut(s) 39, 266
NdeII GATC 2 cut(s) 246, 417
NlaIV GGNNCC 2 cut(s) 384, 419
NmuCI GTSAC 2 cut(s) 392, 560
PciSI GCTCTTC 1 cut(s) 398
PfeI GAWTC 2 cut(s) 353, 442
PkrI GCNGC 2 cut(s) 35, 484
Ppu21I YACGTR 2 cut(s) 363, 504
Psp6I CCWGG 1 cut(s) 47
PspGI CCWGG 1 cut(s) 47
PspN4I GGNNCC 2 cut(s) 384, 419
PspPI GGNCC 2 cut(s) 80, 484
PsuI RGATCY 1 cut(s) 417
SapI GCTCTTC 1 cut(s) 398
SaqAI TTAA 2 cut(s) 399, 495
SatI GCNGC 2 cut(s) 34, 483
Sau3AI GATC 2 cut(s) 246, 417
Sau96I GGNCC 2 cut(s) 80, 484
ScrFI CCNGG 1 cut(s) 49
SetI ASST 8 cut(s) 136, 154, 255, 301, 365, 410, 506, 531
Sse9I AATT 3 cut(s) 188, 221, 324
SsiI CCGC 3 cut(s) 260, 340, 482
SspMI CTAG 2 cut(s) 77, 437
StyD4I CCNGG 1 cut(s) 47
StyI CCWWGG 2 cut(s) 83, 180
TaaI ACNGT 2 cut(s) 373, 476
TaiI ACGT 3 cut(s) 365, 506, 531
TasI AATT 3 cut(s) 188, 221, 324
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 2 cut(s) 353, 442
Tru1I TTAA 2 cut(s) 399, 495
Tru9I TTAA 2 cut(s) 399, 495
TscAI CASTG 3 cut(s) 22, 378, 481
TseFI GTSAC 2 cut(s) 392, 560
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 2 cut(s) 392, 560
TspDTI ATGAA 2 cut(s) 16, 359
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 3 cut(s) 22, 378, 481
XapI RAATTY 2 cut(s) 188, 324
XmiI GTMKAC 1 cut(s) 368
XspI CTAG 2 cut(s) 77, 437
ZraI GACGTC 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.