RchiOBHm_Chr3g0491411

Belongs to the BI1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0018996
Physical Location & Seq
Forward (+)
38150414 .. 38151139
726 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 726 bp
ATGATGAACTGCTGGTCCTTCGTTGGCAAAGTGTACGCCATCCTCACCCTTCAGTTACTCGCCACCATCGGCATCGGCGCCACCGTCAGACTGGACCCTTCAATTTCCCACTTCTTGCTCAAAACCCACGCCGGCATTGCCGTCTACGTCCTCATCCTCGTGACGCCCTTTGTCGTGCTGATCCCAATGCTTTGTCTCAGACACCTCAAGCCGGTTAACTACTTGTTGGTTGCAATTTTCACAGTCTCTCTTGCATTCCAAGTCGGAATTACTTGTGTTTTCACTCCTGGGATGGTGATTTTGGAGTCTGTGTTTTTAACCGCTGTGGTTTTTGTTACTCTCACACTATACACATTCTGGGCTGCTTGGAGAGGCTATGACTTTAGCTTACTAGGACCTTTCCTGTCTGAAGCCTTGCTTTGTCTGGTTATCTATGCTTTCATTCAGATATTTTACCCTCTGGGTAAGACCAAGGTGATGATATTTGGGGCCTTGGGATCCATTGTTTTCTGCGGCTATATCGTATATGACACCTACATGCTGATTGAGCGCCACTCACAAGACAATGACTACATTTTAGCTGCAGTCGGCTTGTACCTGGACATCCTTAATCTTTTCAGCTTCCTTATTGATTTTCTAAGCGGCTGTGATAATGGCTCCTGCGATTGCTGCAGTGCCGAATGCTGCGAGTGCCTAGGAGATATTGGTGGGGAATGTTTAGCTTAA

Protein Analysis

241

Amino Acids

26.55

Weight (kDa)

4.82

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bax1-I PF01027 6 - 212 5e-36 Inhibitor of apoptosis-promoting Bax1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018996)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0491411
rosa_laevigata RLG00000022843
rosa_multiflora Rmu_sc0006034.1_g000003 Rmu_sc0013435.1_g000001
rosa_roxburghii Rroxscaffold_6G00392920
rosa_rugosa Rorug03G0249200
rosa_samantha Rh3CG331500 Rh3DG333000
rosa_wichuraiana Rw3G026350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccI GTMKAC 1 cut(s) 144
AciI CCGC 3 cut(s) 321, 513, 642
AclWI GGATC 3 cut(s) 175, 492, 505
AcuI CTGAAG 2 cut(s) 35, 429
AcyI GRCGYC 2 cut(s) 78, 164
AfaI GTAC 2 cut(s) 35, 596
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 1 cut(s) 102
AjnI CCWGG 2 cut(s) 286, 597
AloI GAACNNNNNNTCC 1 cut(s) 31
AluBI AGCT 4 cut(s) 387, 581, 621, 722
AluI AGCT 4 cut(s) 387, 581, 621, 722
Alw26I GTCTC 2 cut(s) 200, 250
AlwI GGATC 3 cut(s) 175, 492, 505
AoxI GGCC 1 cut(s) 489
ApeKI GCWGC 4 cut(s) 362, 581, 669, 684
AspA2I CCTAGG 1 cut(s) 694
AspLEI GCGC 2 cut(s) 80, 552
AspS9I GGNCC 4 cut(s) 15, 94, 395, 489
AsuHPI GGTGA 3 cut(s) 37, 307, 487
AvaII GGWCC 3 cut(s) 15, 94, 395
AvrII CCTAGG 1 cut(s) 694
BamHI GGATCC 1 cut(s) 497
BanI GGYRCC 1 cut(s) 77
BauI CACGAG 1 cut(s) 158
BbvI GCAGC 4 cut(s) 349, 568, 656, 671
BccI CCATC 3 cut(s) 47, 74, 286
BceAI ACGGC 1 cut(s) 125
BciT130I CCWGG 2 cut(s) 288, 599
BcoDI GTCTC 2 cut(s) 200, 250
BfaI CTAG 2 cut(s) 392, 695
BfmI CTRYAG 2 cut(s) 582, 670
BfoI RGCGCY 2 cut(s) 81, 553
BisI GCNGC 6 cut(s) 363, 514, 582, 643, 670, 685
BlnI CCTAGG 1 cut(s) 694
BlsI GCNGC 6 cut(s) 364, 515, 583, 644, 671, 686
Bme1390I CCNGG 2 cut(s) 288, 599
Bme18I GGWCC 3 cut(s) 15, 94, 395
BmgT120I GGNCC 4 cut(s) 15, 94, 395, 489
BmiI GGNNCC 5 cut(s) 79, 96, 490, 499, 658
BmrFI CCNGG 2 cut(s) 288, 599
BmsI GCATC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 539, 571
BpuEI CTTGAG 1 cut(s) 191
BsaHI GRCGYC 2 cut(s) 78, 164
BsaJI CCNNGG 4 cut(s) 287, 471, 492, 694
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse118I RCCGGY 2 cut(s) 131, 211
Bse1I ACTGG 1 cut(s) 96
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 2 cut(s) 288, 599
BseDI CCNNGG 4 cut(s) 287, 471, 492, 694
BseGI GGATG 4 cut(s) 39, 153, 297, 603
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 1 cut(s) 211
BseNI ACTGG 1 cut(s) 96
BseXI GCAGC 4 cut(s) 349, 568, 656, 671
BshFI GGCC 1 cut(s) 491
BshNI GGYRCC 1 cut(s) 77
BsiSI CCGG 2 cut(s) 132, 212
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 2 cut(s) 200, 250
BsmI GAATGC 2 cut(s) 254, 686
BsnI GGCC 1 cut(s) 491
Bsp143I GATC 2 cut(s) 180, 497
BspACI CCGC 3 cut(s) 321, 513, 642
BspANI GGCC 1 cut(s) 491
BspCNI CTCAG 1 cut(s) 210
BspLI GGNNCC 5 cut(s) 79, 96, 490, 499, 658
BspMAI CTGCAG 2 cut(s) 586, 674
BspPI GGATC 3 cut(s) 175, 492, 505
BspT107I GGYRCC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 135
BsrFI RCCGGY 2 cut(s) 131, 211
BsrI ACTGG 1 cut(s) 96
BssAI RCCGGY 2 cut(s) 131, 211
BssECI CCNNGG 4 cut(s) 287, 471, 492, 694
BssMI GATC 2 cut(s) 180, 497
BssNI GRCGYC 2 cut(s) 78, 164
BssSI CACGAG 1 cut(s) 158
BssT1I CCWWGG 3 cut(s) 471, 492, 694
Bst2BI CACGAG 1 cut(s) 158
Bst2UI CCWGG 2 cut(s) 288, 599
Bst4CI ACNGT 2 cut(s) 85, 244
BstACI GRCGYC 2 cut(s) 78, 164
BstC8I GCNNGC 1 cut(s) 133
BstDEI CTNAG 2 cut(s) 197, 638
BstF5I GGATG 4 cut(s) 39, 153, 297, 603
BstH2I RGCGCY 2 cut(s) 81, 553
BstHHI GCGC 2 cut(s) 80, 552
BstKTI GATC 2 cut(s) 183, 500
BstMAI GTCTC 2 cut(s) 200, 250
BstMBI GATC 2 cut(s) 180, 497
BstMWI GCNNNNNNNGC 4 cut(s) 137, 547, 669, 690
BstNI CCWGG 2 cut(s) 288, 599
BstNSI RCATGY 1 cut(s) 541
BstSCI CCNGG 2 cut(s) 286, 597
BstSFI CTRYAG 2 cut(s) 582, 670
BstV1I GCAGC 4 cut(s) 349, 568, 656, 671
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
BsuRI GGCC 1 cut(s) 491
BtsCI GGATG 4 cut(s) 39, 153, 297, 603
BtsI GCAGTG 1 cut(s) 679
BtsIMutI CAGTG 1 cut(s) 679
Cac8I GCNNGC 1 cut(s) 133
CfoI GCGC 2 cut(s) 80, 552
Cfr10I RCCGGY 2 cut(s) 131, 211
Cfr13I GGNCC 4 cut(s) 15, 94, 395, 489
CseI GACGC 1 cut(s) 172
Csp6I GTAC 2 cut(s) 34, 595
CviAII CATG 1 cut(s) 538
CviQI GTAC 2 cut(s) 34, 595
DdeI CTNAG 2 cut(s) 197, 638
DinI GGCGCC 1 cut(s) 79
DpnI GATC 2 cut(s) 182, 499
DpnII GATC 2 cut(s) 180, 497
Eco130I CCWWGG 3 cut(s) 471, 492, 694
Eco47I GGWCC 3 cut(s) 15, 94, 395
Eco57I CTGAAG 2 cut(s) 35, 429
EcoO109I RGGNCCY 2 cut(s) 395, 489
EcoRII CCWGG 2 cut(s) 286, 597
EcoT14I CCWWGG 3 cut(s) 471, 492, 694
EgeI GGCGCC 1 cut(s) 79
EheI GGCGCC 1 cut(s) 79
ErhI CCWWGG 3 cut(s) 471, 492, 694
FaeI CATG 1 cut(s) 541
FaiI YATR 7 cut(s) 349, 378, 435, 519, 526, 528, 539
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FatI CATG 1 cut(s) 537
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 6 cut(s) 363, 514, 582, 643, 670, 685
FokI GGATG 4 cut(s) 26, 140, 304, 590
Fsp4HI GCNGC 6 cut(s) 363, 514, 582, 643, 670, 685
FspBI CTAG 2 cut(s) 392, 695
GlaI GCGC 2 cut(s) 79, 551
GluI GCNGC 6 cut(s) 363, 514, 582, 643, 670, 685
HaeII RGCGCY 2 cut(s) 81, 553
HaeIII GGCC 1 cut(s) 491
HapII CCGG 2 cut(s) 132, 212
HgaI GACGC 1 cut(s) 172
HhaI GCGC 2 cut(s) 80, 552
Hin1I GRCGYC 2 cut(s) 78, 164
Hin1II CATG 1 cut(s) 541
Hin6I GCGC 2 cut(s) 78, 550
HinP1I GCGC 2 cut(s) 78, 550
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 1 cut(s) 305
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 2 cut(s) 132, 212
HphI GGTGA 3 cut(s) 37, 307, 487
Hpy166II GTNNAC 3 cut(s) 34, 145, 217
Hpy188I TCNGA 5 cut(s) 89, 200, 266, 409, 447
Hpy188III TCNNGA 1 cut(s) 160
Hpy8I GTNNAC 3 cut(s) 34, 145, 217
HpyAV CCTTC 3 cut(s) 28, 59, 108
HpyCH4III ACNGT 2 cut(s) 85, 244
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 4 cut(s) 233, 254, 584, 672
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 547, 669, 690
HpyF3I CTNAG 2 cut(s) 197, 638
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 2 cut(s) 78, 164
Hsp92II CATG 1 cut(s) 541
HspAI GCGC 2 cut(s) 78, 550
KasI GGCGCC 1 cut(s) 77
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
KspAI GTTAAC 1 cut(s) 217
Kzo9I GATC 2 cut(s) 180, 497
LmnI GCTCC 1 cut(s) 662
Lsp1109I GCAGC 4 cut(s) 349, 568, 656, 671
LweI GCATC 1 cut(s) 81
MaeI CTAG 2 cut(s) 392, 695
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 54, 160, 334
MalI GATC 2 cut(s) 182, 499
MboI GATC 2 cut(s) 180, 497
MflI RGATCY 1 cut(s) 497
MluCI AATT 3 cut(s) 102, 234, 267
Mly113I GGCGCC 1 cut(s) 78
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 6 cut(s) 53, 161, 167, 215, 365, 468
MroNI GCCGGC 1 cut(s) 131
MseI TTAA 4 cut(s) 216, 317, 609, 724
MslI CAYNNNNRTG 2 cut(s) 158, 536
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 2 cut(s) 132, 212
MspR9I CCNGG 2 cut(s) 288, 599
Mva1269I GAATGC 2 cut(s) 254, 686
MvaI CCWGG 2 cut(s) 288, 599
MwoI GCNNNNNNNGC 4 cut(s) 137, 547, 669, 690
NaeI GCCGGC 1 cut(s) 133
NarI GGCGCC 1 cut(s) 78
NdeII GATC 2 cut(s) 180, 497
NgoMIV GCCGGC 1 cut(s) 131
NlaIII CATG 1 cut(s) 541
NlaIV GGNNCC 5 cut(s) 79, 96, 490, 499, 658
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 2 cut(s) 254, 686
PdiI GCCGGC 1 cut(s) 133
PkrI GCNGC 6 cut(s) 364, 515, 583, 644, 671, 686
PleI GAGTC 1 cut(s) 313
PluTI GGCGCC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 395
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 2 cut(s) 286, 597
PspGI CCWGG 2 cut(s) 286, 597
PspN4I GGNNCC 5 cut(s) 79, 96, 490, 499, 658
PspPI GGNCC 4 cut(s) 15, 94, 395, 489
PspPPI RGGWCCY 1 cut(s) 395
PstI CTGCAG 2 cut(s) 586, 674
PsuI RGATCY 1 cut(s) 497
RsaI GTAC 2 cut(s) 35, 596
RsaNI GTAC 2 cut(s) 34, 595
RseI CAYNNNNRTG 2 cut(s) 158, 536
SaqAI TTAA 4 cut(s) 216, 317, 609, 724
SatI GCNGC 6 cut(s) 363, 514, 582, 643, 670, 685
Sau3AI GATC 2 cut(s) 180, 497
Sau96I GGNCC 4 cut(s) 15, 94, 395, 489
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 2 cut(s) 288, 599
SfaNI GCATC 1 cut(s) 81
SfcI CTRYAG 2 cut(s) 582, 670
SfoI GGCGCC 1 cut(s) 79
SinI GGWCC 3 cut(s) 15, 94, 395
SmiMI CAYNNNNRTG 2 cut(s) 158, 536
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 3 cut(s) 102, 234, 267
SsiI CCGC 3 cut(s) 321, 513, 642
SspDI GGCGCC 1 cut(s) 77
SspMI CTAG 2 cut(s) 392, 695
StyD4I CCNGG 2 cut(s) 286, 597
StyI CCWWGG 3 cut(s) 471, 492, 694
TaaI ACNGT 2 cut(s) 85, 244
TaiI ACGT 1 cut(s) 150
TasI AATT 3 cut(s) 102, 234, 267
TauI GCSGC 2 cut(s) 516, 645
Tru1I TTAA 4 cut(s) 216, 317, 609, 724
Tru9I TTAA 4 cut(s) 216, 317, 609, 724
TscAI CASTG 1 cut(s) 679
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 4 cut(s) 362, 581, 669, 684
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 2 cut(s) 20, 430
TspRI CASTG 1 cut(s) 679
VpaK11BI GGWCC 3 cut(s) 15, 94, 395
XceI RCATGY 1 cut(s) 541
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XmaJI CCTAGG 1 cut(s) 694
XmiI GTMKAC 1 cut(s) 144
XspI CTAG 2 cut(s) 392, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.