RchiOBHm_Chr2g0097111

Pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0020397
Physical Location & Seq
Forward (+)
9821579 .. 9823770
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 885 bp
ATGCCAGGCCTACTCCAGAGTCCAAAAGCTCATTGCTCCCCAATCCGACCCTCTCCTCGCCAAAGAGATCTTCGACTTCGCCGCCCGCCACCCCCATTTCCGTCACTCCTACTCCTCATACTTCACCCTCATCCTCAAGCTCGCCCACTCCCACTACTTCTCTCTCGTCGACCACCTCCTCCTCCGCCTCAAGGCCCAAACCTACTGTGCAGGAAGGGGCAGGTGAACACTGCTGTAGATTTTCTGGAGGATATGATCAACAAATGGTTTGTTCCGGATTCGTTGAGTTATACTAGCTTGTTGAATAGCTTGTGTAGGAAGAAACAGCTGCGTGAGGCGTATAAGCTTCTTCGTAGGATGAAGGTGAGGGGGTGTAACCCTGACATTGTGCATTACAATACTATAATATTGGGGTTTTGTAGAGAAGGGAGGACAGTGGATGCTTGTAAGGTTCTTGAGGATATGGCATCCAATGGGTGCTTGCCAAATTTGGTGTCGTATAGGACTTTGGTTAGTGGGTTGTCTGATCAGGGGATGCTTGATGAGGCGAAGAACTATATGGAGACAATGATATCAAAGGGGTTTTCTCCACATTTTTCGGTAATCCATGCTTTGGTTAAGGGGTTCTGCAATGTGGGTAGGGTCGAGGAAGCTGGTGGAGTTATGGAGGAGATACCAAGTCATGGGGAGGTTCCGCACAGGGAGACTTGGTTGACAATAGTTCCGGGTATATGCGAAGAGGTTGAGCTGGTGAGAATGGAAGAAGTTCCGAAGGAAATAATGAAGGTAGAGATCAAGCCTAGCACCAGAATAGTGGAGGCAGCTTTTGGATTGGAGAACTACTTGATCAAAAAAATGCATGCTAGTAAATCAAGGAGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

294

Amino Acids

33.16

Weight (kDa)

9.31

Isoelectric Point (pI)

59.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 68 - 106 1.1e-08 PPR repeat family
PPR_3 PF13812 82 - 136 1.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 91 - 121 2.5e-08 PPR repeat
PPR_2 PF13041 92 - 129 2.4e-10 PPR repeat family
PPR PF01535 96 - 125 2e-07 PPR repeat
PPR_1 PF12854 123 - 155 1.1e-12 PPR repeat
PPR_3 PF13812 125 - 171 1.1e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 127 - 175 8.1e-15 PPR repeat family
PPR PF01535 130 - 160 3e-06 PPR repeat
PPR_1 PF12854 158 - 190 3.8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020397)

Species Orthologous Gene IDs
malus_domestica MD07G1160100.v1.1
pyrus_communis pycom07g15640
rosa_chinensis RchiOBHm_Chr2g0097111
rosa_samantha Rh2BG113400 Rh2CG115700 Rh2DG114700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 211
Acc36I ACCTGC 1 cut(s) 211
AccB7I CCANNNNNTGG 2 cut(s) 613, 683
AccI GTMKAC 1 cut(s) 169
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 4 cut(s) 82, 86, 185, 695
AcsI RAATTY 1 cut(s) 487
AfiI CCNNNNNNNGG 3 cut(s) 191, 613, 683
AgsI TTSAA 1 cut(s) 304
AjnI CCWGG 1 cut(s) 4
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AluBI AGCT 9 cut(s) 29, 140, 297, 309, 328, 346, 653, 748, 824
AluI AGCT 9 cut(s) 29, 140, 297, 309, 328, 346, 653, 748, 824
Alw26I GTCTC 2 cut(s) 557, 698
Aor13HI TCCGGA 1 cut(s) 274
AoxI GGCC 2 cut(s) 7, 193
ApeKI GCWGC 2 cut(s) 328, 821
ApoI RAATTY 1 cut(s) 487
ArsI GACNNNNNNTTYG 2 cut(s) 479, 511
Asp700I GAANNNNTTC 1 cut(s) 765
AspS9I GGNCC 1 cut(s) 194
AsuC2I CCSGG 1 cut(s) 726
AsuHPI GGTGA 4 cut(s) 116, 235, 376, 763
BbvI GCAGC 2 cut(s) 315, 833
BciT130I CCWGG 1 cut(s) 6
BclI TGATCA 3 cut(s) 255, 526, 846
BcnI CCSGG 1 cut(s) 726
BcoDI GTCTC 2 cut(s) 557, 698
BfaI CTAG 3 cut(s) 294, 801, 864
BfmI CTRYAG 1 cut(s) 234
BfuAI ACCTGC 1 cut(s) 211
BglII AGATCT 1 cut(s) 67
BisI GCNGC 3 cut(s) 82, 329, 822
BlsI GCNGC 3 cut(s) 83, 330, 823
Bme1390I CCNGG 2 cut(s) 6, 726
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 693
BmrFI CCNGG 2 cut(s) 6, 726
BmsI GCATC 3 cut(s) 430, 476, 525
BpmI CTGGAG 1 cut(s) 266
BpuEI CTTGAG 3 cut(s) 120, 174, 476
BpuMI CCSGG 1 cut(s) 726
BsaWI WCCGGW 1 cut(s) 274
BsaXI ACNNNNNCTCC 4 cut(s) 96, 126, 695, 725
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 613, 683
Bse3DI GCAATG 2 cut(s) 31, 637
BseAI TCCGGA 1 cut(s) 274
BseBI CCWGG 1 cut(s) 6
BseGI GGATG 5 cut(s) 130, 363, 445, 467, 540
BseLI CCNNNNNNNGG 3 cut(s) 191, 613, 683
BseMI GCAATG 2 cut(s) 31, 637
BseRI GAGGAG 5 cut(s) 45, 104, 168, 171, 683
BseXI GCAGC 2 cut(s) 315, 833
BsgI GTGCAG 1 cut(s) 229
BshFI GGCC 2 cut(s) 9, 195
BsiSI CCGG 2 cut(s) 275, 725
BslI CCNNNNNNNGG 3 cut(s) 191, 613, 683
BsmAI GTCTC 2 cut(s) 557, 698
BsnI GGCC 2 cut(s) 9, 195
Bsp13I TCCGGA 1 cut(s) 274
Bsp143I GATC 5 cut(s) 67, 255, 526, 792, 846
BspACI CCGC 4 cut(s) 82, 86, 185, 695
BspANI GGCC 2 cut(s) 9, 195
BspEI TCCGGA 1 cut(s) 274
BspLI GGNNCC 1 cut(s) 693
BspMI ACCTGC 1 cut(s) 211
BsrDI GCAATG 2 cut(s) 31, 637
BssMI GATC 5 cut(s) 67, 255, 526, 792, 846
Bst2UI CCWGG 1 cut(s) 6
Bst4CI ACNGT 2 cut(s) 207, 436
Bst6I CTCTTC 1 cut(s) 732
BstC8I GCNNGC 4 cut(s) 86, 142, 482, 861
BstF5I GGATG 5 cut(s) 130, 363, 445, 467, 540
BstKTI GATC 5 cut(s) 70, 258, 529, 795, 849
BstMAI GTCTC 2 cut(s) 557, 698
BstMBI GATC 5 cut(s) 67, 255, 526, 792, 846
BstNI CCWGG 1 cut(s) 6
BstNSI RCATGY 1 cut(s) 863
BstSCI CCNGG 2 cut(s) 4, 724
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 2 cut(s) 315, 833
BstX2I RGATCY 1 cut(s) 67
BstXI CCANNNNNNTGG 1 cut(s) 814
BstYI RGATCY 1 cut(s) 67
BsuRI GGCC 2 cut(s) 9, 195
BtsCI GGATG 5 cut(s) 130, 363, 445, 467, 540
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 2 cut(s) 228, 441
BveI ACCTGC 1 cut(s) 211
Cac8I GCNNGC 4 cut(s) 86, 142, 482, 861
Cfr13I GGNCC 1 cut(s) 194
CviAII CATG 3 cut(s) 608, 683, 860
DpnI GATC 5 cut(s) 69, 257, 528, 794, 848
DpnII GATC 5 cut(s) 67, 255, 526, 792, 846
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
EciI GGCGGA 1 cut(s) 174
Eco147I AGGCCT 1 cut(s) 9
Eco32I GATATC 1 cut(s) 573
EcoRII CCWGG 1 cut(s) 4
EcoRV GATATC 1 cut(s) 573
EcoT22I ATGCAT 1 cut(s) 861
FaeI CATG 3 cut(s) 611, 686, 863
FatI CATG 3 cut(s) 607, 682, 859
FauI CCCGC 1 cut(s) 93
FbaI TGATCA 3 cut(s) 255, 526, 846
FblI GTMKAC 1 cut(s) 169
Fnu4HI GCNGC 3 cut(s) 82, 329, 822
FokI GGATG 5 cut(s) 117, 370, 452, 454, 547
Fsp4HI GCNGC 3 cut(s) 82, 329, 822
FspBI CTAG 3 cut(s) 294, 801, 864
GluI GCNGC 3 cut(s) 82, 329, 822
GsuI CTGGAG 1 cut(s) 266
HaeIII GGCC 2 cut(s) 9, 195
HapII CCGG 2 cut(s) 275, 725
Hin1II CATG 3 cut(s) 611, 686, 863
HincII GTYRAC 2 cut(s) 170, 714
HindII GTYRAC 2 cut(s) 170, 714
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 2 cut(s) 19, 278
HpaII CCGG 2 cut(s) 275, 725
HphI GGTGA 4 cut(s) 116, 235, 376, 763
Hpy166II GTNNAC 3 cut(s) 170, 226, 714
Hpy188I TCNGA 3 cut(s) 47, 526, 771
Hpy188III TCNNGA 4 cut(s) 16, 245, 275, 455
Hpy8I GTNNAC 3 cut(s) 170, 226, 714
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 5 cut(s) 208, 355, 419, 766, 778
HpyCH4III ACNGT 2 cut(s) 207, 436
HpyCH4V TGCA 4 cut(s) 210, 391, 630, 859
Hsp92II CATG 3 cut(s) 611, 686, 863
Kpn2I TCCGGA 1 cut(s) 274
Ksp22I TGATCA 3 cut(s) 255, 526, 846
Kzo9I GATC 5 cut(s) 67, 255, 526, 792, 846
LmnI GCTCC 1 cut(s) 41
Lsp1109I GCAGC 2 cut(s) 315, 833
LweI GCATC 3 cut(s) 430, 476, 525
MaeI CTAG 3 cut(s) 294, 801, 864
MaeIII GTNAC 2 cut(s) 102, 374
MalI GATC 5 cut(s) 69, 257, 528, 794, 848
MboI GATC 5 cut(s) 67, 255, 526, 792, 846
MboII GAAGA 6 cut(s) 62, 331, 341, 562, 749, 773
MflI RGATCY 1 cut(s) 67
MluCI AATT 1 cut(s) 487
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 1 cut(s) 70
Mph1103I ATGCAT 1 cut(s) 861
MroI TCCGGA 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 765
MseI TTAA 1 cut(s) 618
MspA1I CMGCKG 1 cut(s) 328
MspI CCGG 2 cut(s) 275, 725
MspR9I CCNGG 2 cut(s) 6, 726
MvaI CCWGG 1 cut(s) 6
NciI CCSGG 1 cut(s) 726
NdeII GATC 5 cut(s) 67, 255, 526, 792, 846
NlaIII CATG 3 cut(s) 611, 686, 863
NlaIV GGNNCC 1 cut(s) 693
NmuCI GTSAC 1 cut(s) 102
NsiI ATGCAT 1 cut(s) 861
NspI RCATGY 1 cut(s) 863
PaeI GCATGC 1 cut(s) 863
PaqCI CACCTGC 1 cut(s) 211
PceI AGGCCT 1 cut(s) 9
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 1 cut(s) 278
PflMI CCANNNNNTGG 2 cut(s) 613, 683
PkrI GCNGC 3 cut(s) 83, 330, 823
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 4
PspGI CCWGG 1 cut(s) 4
PspN4I GGNNCC 1 cut(s) 693
PspPI GGNCC 1 cut(s) 194
PsuI RGATCY 1 cut(s) 67
PvuII CAGCTG 1 cut(s) 328
SalI GTCGAC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 3 cut(s) 82, 329, 822
Sau3AI GATC 5 cut(s) 67, 255, 526, 792, 846
Sau96I GGNCC 1 cut(s) 194
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 2 cut(s) 6, 726
SfaNI GCATC 3 cut(s) 430, 476, 525
SfcI CTRYAG 1 cut(s) 234
SmlI CTYRAG 3 cut(s) 135, 189, 455
SmoI CTYRAG 3 cut(s) 135, 189, 455
SphI GCATGC 1 cut(s) 863
Sse9I AATT 1 cut(s) 487
SseBI AGGCCT 1 cut(s) 9
SsiI CCGC 4 cut(s) 82, 86, 185, 695
SspI AATATT 1 cut(s) 408
SspMI CTAG 3 cut(s) 294, 801, 864
StuI AGGCCT 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 4, 724
TaaI ACNGT 2 cut(s) 207, 436
TaqI TCGA 3 cut(s) 73, 169, 645
TasI AATT 1 cut(s) 487
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 2 cut(s) 235, 441
TseFI GTSAC 1 cut(s) 102
TseI GCWGC 2 cut(s) 328, 821
Tsp45I GTSAC 1 cut(s) 102
TspDTI ATGAA 2 cut(s) 374, 797
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 2 cut(s) 235, 441
Van91I CCANNNNNTGG 2 cut(s) 613, 683
XapI RAATTY 1 cut(s) 487
XceI RCATGY 1 cut(s) 863
XmiI GTMKAC 1 cut(s) 169
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 3 cut(s) 294, 801, 864
Zsp2I ATGCAT 1 cut(s) 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.