RchiOBHm_Chr2g0106301

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0019385
Physical Location & Seq
Forward (+)
17750423 .. 17750887
465 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 465 bp
ATGCGCACTGCACTTATAACCGAATTGCTTTTACTTGAAAACCAGCTTCCTTGGAGAGTTTTTAACTGTTTGCTTCACCTCACCTCCGAAGCTGATAAGTTTCCTCTTCCTAAGCTTACTCTAAAGTACTTTGAGGGGTACACAATGGGGACATATCCACAGGCTAATGCAGCATTAGAAAATAAACATTTGCTGGACTTTTTAGGTAATTGTTTACAAGGATCTTCTGATGAATCTGCAGAGTTTGAGAACTATAGCCAGAGGCGCCCCTCGATTCCCTCTGGGACAGAGCTTGATTATTTTGGTATTCGGTTTAAGCTTGGAGGCAGTAATGACGTGTGCAATGGAACATTCAGGAATGGATTTCTGACGATTCCACCATTATCAGTTCAAGAGAATGGAGAATCTCTCTTTAGAAACCTCAAGCCTATGAACAGTTGGAACCGAGTAATAAGAGTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

154

Amino Acids

17.52

Weight (kDa)

6.12

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 4 - 141 3.5e-22 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019385)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0106301
rosa_laevigata RLG00000017487
rosa_roxburghii Rroxscaffold_2G00137290
rosa_rugosa Rorug02G0134600
rosa_samantha Rh2AG185600 Rh2BG195800 Rh2CG190600 Rh2DG191700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 264
AclWI GGATC 1 cut(s) 229
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 2 cut(s) 128, 140
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 2 cut(s) 38, 392
AjiI CACGTC 1 cut(s) 337
AluBI AGCT 5 cut(s) 46, 92, 115, 292, 319
AluI AGCT 5 cut(s) 46, 92, 115, 292, 319
AlwI GGATC 1 cut(s) 229
ApeKI GCWGC 1 cut(s) 170
AspLEI GCGC 2 cut(s) 6, 267
AsuHPI GGTGA 2 cut(s) 68, 73
BanI GGYRCC 1 cut(s) 264
BbvI GCAGC 1 cut(s) 182
BfmI CTRYAG 2 cut(s) 237, 253
BfoI RGCGCY 1 cut(s) 268
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
BmcAI AGTACT 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 337
BmiI GGNNCC 2 cut(s) 266, 443
BplI GAGNNNNNCTC 2 cut(s) 393, 425
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 407
BsaHI GRCGYC 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bse3DI GCAATG 1 cut(s) 349
BseDI CCNNGG 1 cut(s) 50
BseMI GCAATG 1 cut(s) 349
BseXI GCAGC 1 cut(s) 182
BshNI GGYRCC 1 cut(s) 264
BslFI GGGAC 2 cut(s) 163, 298
BsmFI GGGAC 2 cut(s) 163, 298
Bsp143I GATC 1 cut(s) 221
BspLI GGNNCC 2 cut(s) 266, 443
BspMAI CTGCAG 1 cut(s) 241
BspPI GGATC 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 349
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 1 cut(s) 221
BssNI GRCGYC 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 68, 437
Bst6I CTCTTC 1 cut(s) 111
BstACI GRCGYC 1 cut(s) 265
BstDEI CTNAG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 2 cut(s) 6, 267
BstKTI GATC 1 cut(s) 224
BstMBI GATC 1 cut(s) 221
BstMWI GCNNNNNNNGC 2 cut(s) 170, 264
BstSFI CTRYAG 2 cut(s) 237, 253
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 221
BstYI RGATCY 1 cut(s) 221
BtrI CACGTC 1 cut(s) 337
BtsI GCAGTG 1 cut(s) 6
BtsIMutI CAGTG 1 cut(s) 6
CfoI GCGC 2 cut(s) 6, 267
Csp6I GTAC 2 cut(s) 127, 139
CviJI RGCY 8 cut(s) 46, 92, 115, 164, 258, 292, 319, 427
CviKI_1 RGCY 8 cut(s) 46, 92, 115, 164, 258, 292, 319, 427
CviQI GTAC 2 cut(s) 127, 139
DdeI CTNAG 1 cut(s) 111
DinI GGCGCC 1 cut(s) 266
DpnI GATC 1 cut(s) 223
DpnII GATC 1 cut(s) 221
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 50
EcoT14I CCWWGG 1 cut(s) 50
EgeI GGCGCC 1 cut(s) 266
EheI GGCGCC 1 cut(s) 266
ErhI CCWWGG 1 cut(s) 50
FaiI YATR 4 cut(s) 17, 154, 255, 431
FaqI GGGAC 2 cut(s) 163, 298
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
FspAI RTGCGCAY 1 cut(s) 5
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 2 cut(s) 5, 266
GluI GCNGC 1 cut(s) 171
HaeII RGCGCY 1 cut(s) 268
HhaI GCGC 2 cut(s) 6, 267
Hin1I GRCGYC 1 cut(s) 265
Hin6I GCGC 2 cut(s) 4, 265
HinP1I GCGC 2 cut(s) 4, 265
HindIII AAGCTT 2 cut(s) 113, 317
HinfI GANTC 4 cut(s) 233, 274, 373, 404
HphI GGTGA 2 cut(s) 68, 73
Hpy166II GTNNAC 2 cut(s) 141, 215
Hpy188I TCNGA 3 cut(s) 88, 229, 369
Hpy188III TCNNGA 2 cut(s) 355, 392
Hpy8I GTNNAC 2 cut(s) 141, 215
HpyCH4III ACNGT 2 cut(s) 68, 437
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 4 cut(s) 11, 170, 239, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 264
HpyF3I CTNAG 1 cut(s) 111
HpySE526I ACGT 1 cut(s) 336
Hsp92I GRCGYC 1 cut(s) 265
HspAI GCGC 2 cut(s) 4, 265
KasI GGCGCC 1 cut(s) 264
Kzo9I GATC 1 cut(s) 221
LpnPI CCDG 6 cut(s) 56, 146, 179, 267, 272, 340
Lsp1109I GCAGC 1 cut(s) 182
MaeII ACGT 1 cut(s) 336
MalI GATC 1 cut(s) 223
MboI GATC 1 cut(s) 221
MboII GAAGA 2 cut(s) 98, 216
MflI RGATCY 1 cut(s) 221
MluCI AATT 3 cut(s) 23, 208, 460
Mly113I GGCGCC 1 cut(s) 265
MmeI TCCRAC 1 cut(s) 419
MnlI CCTC 9 cut(s) 89, 94, 114, 127, 255, 280, 289, 317, 431
MseI TTAA 2 cut(s) 63, 315
MwoI GCNNNNNNNGC 2 cut(s) 170, 264
NarI GGCGCC 1 cut(s) 265
NdeII GATC 1 cut(s) 221
NlaIV GGNNCC 2 cut(s) 266, 443
NsbI TGCGCA 1 cut(s) 5
PfeI GAWTC 4 cut(s) 233, 274, 373, 404
PkrI GCNGC 1 cut(s) 172
PluTI GGCGCC 1 cut(s) 268
PsiI TTATAA 1 cut(s) 17
PspN4I GGNNCC 2 cut(s) 266, 443
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 1 cut(s) 221
RsaI GTAC 2 cut(s) 128, 140
RsaNI GTAC 2 cut(s) 127, 139
SaqAI TTAA 2 cut(s) 63, 315
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 1 cut(s) 221
ScaI AGTACT 1 cut(s) 128
SfcI CTRYAG 2 cut(s) 237, 253
SfoI GGCGCC 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 3 cut(s) 23, 208, 460
SspDI GGCGCC 1 cut(s) 264
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 68, 437
TaiI ACGT 1 cut(s) 339
TaqI TCGA 1 cut(s) 272
TasI AATT 3 cut(s) 23, 208, 460
TatI WGTACW 1 cut(s) 126
TfiI GAWTC 4 cut(s) 233, 274, 373, 404
Tru1I TTAA 2 cut(s) 63, 315
Tru9I TTAA 2 cut(s) 63, 315
TscAI CASTG 1 cut(s) 13
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 2 cut(s) 246, 446
TspRI CASTG 1 cut(s) 13
ZrmI AGTACT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.