Rh2AG185600

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0019385
Physical Location & Seq
Forward (+)
18114638 .. 18115204
567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG185600.1

Sequence Viewer

Length: 567 bp
ATGCGCACTGCACTTATAACCGAATTGCTTTTACTTGAAAACCAGCTTCCTTGGAGAGTTTTTAACTGTTTGCTTCACCTCATCTCCGAAGCTGATAAGTTTCCTCTTCCTAAGCTTACTCTAAAGTACTTTGAGGGGTACACAATGGGGACATATCCACAGGCTAATGCAGCAGTAGAAAATCAACATTTGCTGGACTTTTTAGGTAATTGTTTACAAGGATCTTCTGATGAATCTGCAGAGTTTGAGAACTATAGCCAGAGGCGCCCCTCGATTCCCTCTGTGACAGAGCTTGATTATTTTGGTATTCGGTTAAAGCTTGGAGGCAGTAATGACGTGTGCAATGGAACATTCAGGAATGGATTTCTGACGATTCCACCCTTATCAGTTCAAGAGAATGGAGAATCTCTCTTTAGAAACCTCAGAGCCTATGAACAGTTGGAACCGAGTAATAAGAGTAAATTGATCTCTTATGTTGTGTTGTTAGATGACCTTATTAAGTGCGAAAAAGATCTGGCGTCACTTAATGAAAAGGAAATTATCGCAAACTTCGTGCATGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

188

Amino Acids

21.38

Weight (kDa)

5.02

Isoelectric Point (pI)

29.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 4 - 185 1.7e-37 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019385)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0106301
rosa_laevigata RLG00000017487
rosa_roxburghii Rroxscaffold_2G00137290
rosa_rugosa Rorug02G0134600
rosa_samantha Rh2AG185600 Rh2BG195800 Rh2CG190600 Rh2DG191700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 264
AclWI GGATC 1 cut(s) 229
AcyI GRCGYC 2 cut(s) 265, 518
AfaI GTAC 2 cut(s) 128, 140
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 2 cut(s) 38, 392
AjiI CACGTC 1 cut(s) 337
AluBI AGCT 5 cut(s) 46, 92, 115, 292, 319
AluI AGCT 5 cut(s) 46, 92, 115, 292, 319
AlwI GGATC 1 cut(s) 229
ApeKI GCWGC 1 cut(s) 170
AspLEI GCGC 2 cut(s) 6, 267
AsuHPI GGTGA 1 cut(s) 68
BanI GGYRCC 1 cut(s) 264
BbvI GCAGC 1 cut(s) 182
BfmI CTRYAG 2 cut(s) 237, 253
BfoI RGCGCY 1 cut(s) 268
BglII AGATCT 1 cut(s) 511
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
BmcAI AGTACT 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 337
BmiI GGNNCC 2 cut(s) 266, 444
BplI GAGNNNNNCTC 2 cut(s) 393, 425
Bpu10I CCTNAGC 1 cut(s) 111
Bsa29I ATCGAT 1 cut(s) 562
BsaHI GRCGYC 2 cut(s) 265, 518
BsaJI CCNNGG 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bse3DI GCAATG 1 cut(s) 349
BseCI ATCGAT 1 cut(s) 562
BseDI CCNNGG 1 cut(s) 50
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 436
BseXI GCAGC 1 cut(s) 182
BshNI GGYRCC 1 cut(s) 264
BshVI ATCGAT 1 cut(s) 562
BslFI GGGAC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 163
Bsp143I GATC 4 cut(s) 221, 465, 511, 559
BspCNI CTCAG 1 cut(s) 435
BspDI ATCGAT 1 cut(s) 562
BspLI GGNNCC 2 cut(s) 266, 444
BspMAI CTGCAG 1 cut(s) 241
BspPI GGATC 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 349
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 4 cut(s) 221, 465, 511, 559
BssNI GRCGYC 2 cut(s) 265, 518
BssT1I CCWWGG 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 68, 438
Bst6I CTCTTC 1 cut(s) 111
BstACI GRCGYC 2 cut(s) 265, 518
BstDEI CTNAG 2 cut(s) 111, 422
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 2 cut(s) 6, 267
BstKTI GATC 4 cut(s) 224, 468, 514, 562
BstMBI GATC 4 cut(s) 221, 465, 511, 559
BstMWI GCNNNNNNNGC 2 cut(s) 170, 264
BstSFI CTRYAG 2 cut(s) 237, 253
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 2 cut(s) 221, 511
BstYI RGATCY 2 cut(s) 221, 511
Bsu15I ATCGAT 1 cut(s) 562
BsuTUI ATCGAT 1 cut(s) 562
BtrI CACGTC 1 cut(s) 337
BtsI GCAGTG 1 cut(s) 6
BtsIMutI CAGTG 1 cut(s) 6
CfoI GCGC 2 cut(s) 6, 267
ClaI ATCGAT 1 cut(s) 562
CseI GACGC 1 cut(s) 507
Csp6I GTAC 2 cut(s) 127, 139
CviAII CATG 1 cut(s) 557
CviJI RGCY 8 cut(s) 46, 92, 115, 164, 258, 292, 319, 428
CviKI_1 RGCY 8 cut(s) 46, 92, 115, 164, 258, 292, 319, 428
CviQI GTAC 2 cut(s) 127, 139
DdeI CTNAG 2 cut(s) 111, 422
DinI GGCGCC 1 cut(s) 266
DpnI GATC 4 cut(s) 223, 467, 513, 561
DpnII GATC 4 cut(s) 221, 465, 511, 559
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 50
EcoT14I CCWWGG 1 cut(s) 50
EgeI GGCGCC 1 cut(s) 266
EheI GGCGCC 1 cut(s) 266
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 1 cut(s) 560
FaiI YATR 6 cut(s) 17, 154, 255, 432, 474, 558
FaqI GGGAC 1 cut(s) 163
FatI CATG 1 cut(s) 556
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
FspAI RTGCGCAY 1 cut(s) 5
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 2 cut(s) 5, 266
GluI GCNGC 1 cut(s) 171
HaeII RGCGCY 1 cut(s) 268
HgaI GACGC 1 cut(s) 507
HhaI GCGC 2 cut(s) 6, 267
Hin1I GRCGYC 2 cut(s) 265, 518
Hin1II CATG 1 cut(s) 560
Hin6I GCGC 2 cut(s) 4, 265
HinP1I GCGC 2 cut(s) 4, 265
HindIII AAGCTT 2 cut(s) 113, 317
HinfI GANTC 4 cut(s) 233, 274, 373, 404
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 2 cut(s) 141, 215
Hpy188I TCNGA 4 cut(s) 88, 229, 369, 425
Hpy188III TCNNGA 2 cut(s) 355, 392
Hpy8I GTNNAC 2 cut(s) 141, 215
HpyCH4III ACNGT 2 cut(s) 68, 438
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 5 cut(s) 11, 170, 239, 342, 556
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 264
HpyF3I CTNAG 2 cut(s) 111, 422
HpySE526I ACGT 1 cut(s) 336
Hsp92I GRCGYC 2 cut(s) 265, 518
Hsp92II CATG 1 cut(s) 560
HspAI GCGC 2 cut(s) 4, 265
KasI GGCGCC 1 cut(s) 264
Kzo9I GATC 4 cut(s) 221, 465, 511, 559
LpnPI CCDG 6 cut(s) 56, 146, 179, 272, 340, 500
Lsp1109I GCAGC 1 cut(s) 182
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 283, 519
MalI GATC 4 cut(s) 223, 467, 513, 561
MboI GATC 4 cut(s) 221, 465, 511, 559
MboII GAAGA 2 cut(s) 98, 216
MflI RGATCY 2 cut(s) 221, 511
MluCI AATT 4 cut(s) 23, 208, 461, 537
Mly113I GGCGCC 1 cut(s) 265
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 8 cut(s) 89, 114, 127, 255, 280, 289, 317, 431
MseI TTAA 4 cut(s) 63, 314, 498, 525
MwoI GCNNNNNNNGC 2 cut(s) 170, 264
NarI GGCGCC 1 cut(s) 265
NdeII GATC 4 cut(s) 221, 465, 511, 559
NlaIII CATG 1 cut(s) 560
NlaIV GGNNCC 2 cut(s) 266, 444
NmuCI GTSAC 2 cut(s) 283, 519
NsbI TGCGCA 1 cut(s) 5
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PfeI GAWTC 4 cut(s) 233, 274, 373, 404
PkrI GCNGC 1 cut(s) 172
PluTI GGCGCC 1 cut(s) 268
PsiI TTATAA 1 cut(s) 17
PspN4I GGNNCC 2 cut(s) 266, 444
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 221, 511
RsaI GTAC 2 cut(s) 128, 140
RsaNI GTAC 2 cut(s) 127, 139
SaqAI TTAA 4 cut(s) 63, 314, 498, 525
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 4 cut(s) 221, 465, 511, 559
ScaI AGTACT 1 cut(s) 128
SfcI CTRYAG 2 cut(s) 237, 253
SfoI GGCGCC 1 cut(s) 266
Sse9I AATT 4 cut(s) 23, 208, 461, 537
SspDI GGCGCC 1 cut(s) 264
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 68, 438
TaiI ACGT 1 cut(s) 339
TaqI TCGA 2 cut(s) 272, 562
TasI AATT 4 cut(s) 23, 208, 461, 537
TatI WGTACW 1 cut(s) 126
TfiI GAWTC 4 cut(s) 233, 274, 373, 404
Tru1I TTAA 4 cut(s) 63, 314, 498, 525
Tru9I TTAA 4 cut(s) 63, 314, 498, 525
TscAI CASTG 1 cut(s) 13
TseFI GTSAC 2 cut(s) 283, 519
TseI GCWGC 1 cut(s) 170
Tsp45I GTSAC 2 cut(s) 283, 519
TspDTI ATGAA 3 cut(s) 246, 447, 543
TspRI CASTG 1 cut(s) 13
ZrmI AGTACT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.