Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0018359

Physical Location & Seq

Genomic Coordinates Forward (+)

33579731 .. 33581722

Gene Structure 1992 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGGGTCCAATTTCATTGTCTGGACCAACGGAGTTCGATGTAATGAAAAGTAATGAACTTGAAAGGTATTTGCGCAACGATGCCGGGCTTTATGAGTGTAGGGAGGAGTCTCTGATTAGAGAGGAAGTTCTAGGAAGACTTGACGAGATTGTTAAGCAATGGGTTAAAGCAATTAGCCGAGGCAAAGGTTTCGATGAGGAATTTGTAGAAGAAGCAAATGCTAAGGTCTTCACCTTTGGGTCATACAGGCTAGGAGTTCATAGCCCTGGCGCAGATATAGATACACTCTGCGTGGGACCAGCATATGTAACTCGAGAAGAGGGTTTCTTTGGGGAGCTGTATAAGATGTTGTCTGAGATGCCAGAAGAAGTAACCGAGTTGAATCCTGTGCATGATGCTCATGTTCCAGTTCTGAAGTTCAAGTTCAATGGGGTTTCGATAGACCTTGTTTATGCAAGACTATCGCTTCCGGTTATTCCTGAAGACTTAGATTTGTCACAAGACTCAATCCTACAACATGTCAGTGCTGGTGATGAACAAACTGTTCGAAGCCTCAATGGTTGTAGAGTTACAGACCGAATTCTAAAGTTGGTCCCAAACATTCAGACTTTCCGCACAACATTAAGATGCATTAGGTTATGGGCAAAGCATCGTGGTGTTTATTCGAATGTTGCAGGGTTTCTTGGTGGTATAAACTGGGCATTGCTTGTGGCTTTCATATGCCAACTTTACCCAAATGCATTGCCTAGTCTACTAGTGTCTCGGTTCTTCAGGGTCTATACTCAGTGGCGTTGGCCAAATCCAGTATTGCTCTGCCCTATCAAAGAAGGGCTTGGACTTCAGGTTTGGGATCCAAGAAGAAATGTAAGGGATAGGTACCATTTGATGCCTATAATTACCCCAGCTTATCCTTCCATGAACTCTAGCTACAATGTGTCGCAGAGTACTTTGTGTATCATGTTAGGAGAGTTTCAGAGGGGAAATGACATTTGTAAGGCCATGGAAGCAGAAAATAAGGATTTTGATTGGGGTACCCTTTTCGAGTCATATGCTTTCTTTGACGCGTATAAGAATTACTTGCAGATAGACATATGTGCAGGAGGTGCTGATGACTTCAGGAAATGGAAAGGCTGGGTTGAATCTCGGATCCGTCAACTAACACTCAAAGTTGAGAATGAAACTGGCGGCATGTTGCAGTGTCACCCACACCCTGGTGATTTTCCAGACAAGACCCTACCTTATCATAGCTCATTCTTCATGGGACTACAGCGTACCCAACAAGTTCCGGTCAAAGGAGGTGAGCCGGTTCATTTAGGGAGATGTGTTGATGAATTTAAGTGGGCTGTGAATGCTTATACTTCAAGGAAGACTGGAATGGACATTCGTGTATCCCATGTAAAGCGCAGGGACATCCCTAATTTTGTATTTCCTGGAGGGATCCGACGTTTGCGTACATCTAAAGCAACTTGGGAGAGAAGGCAACGTTCCCCTCAACCGGAGGCTTCAGGCGACATAGCTCAATGTCAGTCTAAAACAACTGTTCTAGATGGGACAGATGGAGGTCAGAAGAGAAAAAGGCAGGAGAATGGTGATGCAGAGACCGACTCCTCATCAAGGCACCCCAAGTCTGTGAGGTGCAACCCGCCGATCAACACTGTTGTCGCCGATGCATCAATTTCTAAACAAGCAGAGAAGCTGGCACGGGAGCCACATGTTCCGGACCAAGTTTTGCCAAAAGAACTGGATGAGCTTGAAGATGATAGAGATCAAGTCAAAGACTTTGCTGGTGGGAACATGAAGGATAGTCACACAATGGGGGCAATAAAATCTAACAATGGAGCTGGTCCTTCCACTGCTTGTTCATATATTGGGGACTTGGAGGAACTTGAGTTTAATGAACTTAAGGTGCCGTACTATCAAACCCCTTCTGAACCTGTGGCGAAGCCAATCATTAGAGTGAATTTCATTACATTGGCCAGTGGAACATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004652 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008285 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0022414 GO:0031123 GO:0031124 GO:0031347 GO:0031348 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045824 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048441 GO:0048444 GO:0048446 GO:0048449 GO:0048451 GO:0048465 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0070013 GO:0070566 GO:0071704 GO:0080134 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901360 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

NTP_transf_2 PAP_RNA-bind PAP_central

Protein Analysis

663

Amino Acids

74.82

Weight (kDa)

6.22

Isoelectric Point (pI)

42.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAP_NTPase	PF20750	3 - 198	2.4e-67	Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2	PF01909	67 - 151	9e-11	Nucleotidyltransferase domain
PAP_central	PF04928	203 - 348	5.4e-63	Poly(A) polymerase central domain
PAP_RNA-bind	PF04926	352 - 409	1.6e-10	Poly(A) polymerase predicted RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0018359)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g24260
rosa_chinensis	RchiOBHm_Chr2g0120741
rosa_laevigata	RLG00000018576
rosa_multiflora	Rmu_sc0000319.1_g000010
rosa_rugosa	Rorug02G0230600
rosa_samantha	Rh2AG287300 Rh2BG298800 Rh2CG276700 Rh2DG312600
rosa_wichuraiana	Rw2G023040

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	74
Acc65I	GGTACC	2 cut(s)	876, 1031
AccB1I	GGYRCC	4 cut(s)	876, 1031, 1617, 1906
AccB7I	CCANNNNNTGG	1 cut(s)	1211
AccI	GTMKAC	1 cut(s)	751
AccII	CGCG	1 cut(s)	1064
AccIII	TCCGGA	1 cut(s)	1717
AciI	CCGC	3 cut(s)	613, 1185, 1643
AclI	AACGTT	1 cut(s)	1483
AclWI	GGATC	6 cut(s)	845, 858, 1141, 1154, 1432, 1445
AcoI	YGGCCR	2 cut(s)	794, 1974
AcsI	RAATTY	4 cut(s)	200, 579, 1331, 1960
AcuI	CTGAAG	6 cut(s)	434, 501, 754, 824, 1099, 1488
AfaI	GTAC	6 cut(s)	878, 946, 1033, 1273, 1453, 1913
AfiI	CCNNNNNNNGG	4 cut(s)	1211, 1292, 1495, 1614
AflII	CTTAAG	1 cut(s)	1901
AflIII	ACRYGT	4 cut(s)	517, 1062, 1711, 1985
AgsI	TTSAA	7 cut(s)	62, 382, 421, 427, 1139, 1362, 1754
AhlI	ACTAGT	1 cut(s)	754
AjnI	CCWGG	3 cut(s)	265, 1210, 1429
AleI	CACNNNNGTG	1 cut(s)	1212
AluBI	AGCT	8 cut(s)	337, 905, 927, 1248, 1517, 1696, 1750, 1841
AluI	AGCT	8 cut(s)	337, 905, 927, 1248, 1517, 1696, 1750, 1841
Alw26I	GTCTC	3 cut(s)	114, 765, 1592
AlwI	GGATC	6 cut(s)	845, 858, 1141, 1154, 1432, 1445
Ama87I	CYCGRG	1 cut(s)	312
Aor13HI	TCCGGA	1 cut(s)	1717
AoxI	GGCC	3 cut(s)	794, 996, 1974
ApoI	RAATTY	4 cut(s)	200, 579, 1331, 1960
ArsI	GACNNNNNNTTYG	2 cut(s)	828, 860
Asp718I	GGTACC	2 cut(s)	876, 1031
AspLEI	GCGC	3 cut(s)	75, 272, 1404
AspS9I	GGNCC	6 cut(s)	5, 23, 296, 592, 1720, 1844
AsuC2I	CCSGG	1 cut(s)	85
AsuHPI	GGTGA	6 cut(s)	223, 542, 1193, 1226, 1310, 1601
AsuII	TTCGAA	2 cut(s)	547, 665
AvaI	CYCGRG	1 cut(s)	312
AvaII	GGWCC	6 cut(s)	5, 23, 296, 592, 1720, 1844
BalI	TGGCCA	2 cut(s)	796, 1976
BamHI	GGATCC	3 cut(s)	850, 1146, 1437
BanI	GGYRCC	4 cut(s)	876, 1031, 1617, 1906
BbsI	GAAGAC	4 cut(s)	142, 220, 489, 1373
BccI	CCATC	2 cut(s)	1541, 1550
BceAI	ACGGC	1 cut(s)	1894
BcgI	CGANNNNNNTGC	6 cut(s)	172, 206, 444, 478, 1031, 1065
BciT130I	CCWGG	3 cut(s)	267, 1212, 1431
BciVI	GTATCC	1 cut(s)	1399
BcnI	CCSGG	1 cut(s)	85
BcoDI	GTCTC	3 cut(s)	114, 765, 1592
BcuI	ACTAGT	1 cut(s)	754
BfaI	CTAG	6 cut(s)	131, 251, 747, 755, 924, 1544
BfmI	CTRYAG	1 cut(s)	1265
BfrI	CTTAAG	1 cut(s)	1901
BfuI	GTATCC	1 cut(s)	1399
BisI	GCNGC	1 cut(s)	1186
BlsI	GCNGC	1 cut(s)	1187
BmcAI	AGTACT	1 cut(s)	946
Bme1390I	CCNGG	4 cut(s)	85, 267, 1212, 1431
Bme18I	GGWCC	6 cut(s)	5, 23, 296, 592, 1720, 1844
BmeT110I	CYCGRG	1 cut(s)	312
BmgT120I	GGNCC	6 cut(s)	5, 23, 296, 592, 1720, 1844
BmrFI	CCNGG	4 cut(s)	85, 267, 1212, 1431
BmrI	ACTGGG	1 cut(s)	706
BmsI	GCATC	9 cut(s)	70, 348, 385, 617, 658, 876, 1582, 1657, 1679
BmuI	ACTGGG	1 cut(s)	706
BpiI	GAAGAC	4 cut(s)	142, 220, 489, 1373
BplI	GAGNNNNNCTC	2 cut(s)	1589, 1621
BpmI	CTGGAG	1 cut(s)	1452
Bpu10I	CCTNAGC	1 cut(s)	222
Bpu14I	TTCGAA	2 cut(s)	547, 665
BpuEI	CTTGAG	1 cut(s)	1907
BpuMI	CCSGG	1 cut(s)	85
BsaBI	GATNNNNATC	1 cut(s)	1764
BsaI	GGTCTC	1 cut(s)	1592
BsaJI	CCNNGG	4 cut(s)	178, 265, 999, 1210
BsaWI	WCCGGW	4 cut(s)	469, 1285, 1495, 1717
Bsc4I	CCNNNNNNNGG	4 cut(s)	1211, 1292, 1495, 1614
Bse118I	RCCGGY	1 cut(s)	1303
Bse1I	ACTGG	7 cut(s)	407, 701, 803, 1186, 1375, 1746, 1977
Bse3DI	GCAATG	3 cut(s)	164, 701, 740
Bse8I	GATNNNNATC	1 cut(s)	1764
BseAI	TCCGGA	1 cut(s)	1717
BseBI	CCWGG	3 cut(s)	267, 1212, 1431
BseDI	CCNNGG	4 cut(s)	178, 265, 999, 1210
BseGI	GGATG	2 cut(s)	1410, 1750
BseJI	GATNNNNATC	1 cut(s)	1764
BseLI	CCNNNNNNNGG	4 cut(s)	1211, 1292, 1495, 1614
BseMI	GCAATG	3 cut(s)	164, 701, 740
BseMII	CTCAG	2 cut(s)	345, 797
BseNI	ACTGG	7 cut(s)	407, 701, 803, 1186, 1375, 1746, 1977
BseRI	GAGGAG	2 cut(s)	119, 1597
BseYI	CCCAGC	2 cut(s)	901, 1131
BsgI	GTGCAG	1 cut(s)	1116
Bsh1236I	CGCG	1 cut(s)	1064
BshFI	GGCC	3 cut(s)	796, 998, 1976
BshNI	GGYRCC	4 cut(s)	876, 1031, 1617, 1906
BsiHKCI	CYCGRG	1 cut(s)	312
BsiSI	CCGG	6 cut(s)	84, 470, 1286, 1304, 1496, 1718
BslFI	GGGAC	6 cut(s)	309, 578, 1275, 1421, 1564, 1886
BslI	CCNNNNNNNGG	4 cut(s)	1211, 1292, 1495, 1614
BsmAI	GTCTC	3 cut(s)	114, 765, 1592
BsmFI	GGGAC	6 cut(s)	309, 578, 1275, 1421, 1564, 1886
BsmI	GAATGC	1 cut(s)	1354
BsnI	GGCC	3 cut(s)	796, 998, 1976
Bso31I	GGTCTC	1 cut(s)	1592
BsoBI	CYCGRG	1 cut(s)	312
Bsp119I	TTCGAA	2 cut(s)	547, 665
Bsp13I	TCCGGA	1 cut(s)	1717
Bsp143I	GATC	5 cut(s)	850, 1146, 1437, 1647, 1765
Bsp19I	CCATGG	1 cut(s)	999
BspACI	CCGC	3 cut(s)	613, 1185, 1643
BspANI	GGCC	3 cut(s)	796, 998, 1976
BspCNI	CTCAG	2 cut(s)	346, 796
BspEI	TCCGGA	1 cut(s)	1717
BspFNI	CGCG	1 cut(s)	1064
BspPI	GGATC	6 cut(s)	845, 858, 1141, 1154, 1432, 1445
BspT104I	TTCGAA	2 cut(s)	547, 665
BspT107I	GGYRCC	4 cut(s)	876, 1031, 1617, 1906
BspTI	CTTAAG	1 cut(s)	1901
BspTNI	GGTCTC	1 cut(s)	1592
BsrDI	GCAATG	3 cut(s)	164, 701, 740
BsrFI	RCCGGY	1 cut(s)	1303
BsrI	ACTGG	7 cut(s)	407, 701, 803, 1186, 1375, 1746, 1977
BssAI	RCCGGY	1 cut(s)	1303
BssECI	CCNNGG	4 cut(s)	178, 265, 999, 1210
BssMI	GATC	5 cut(s)	850, 1146, 1437, 1647, 1765
BssT1I	CCWWGG	1 cut(s)	999
Bst2UI	CCWGG	3 cut(s)	267, 1212, 1431
Bst4CI	ACNGT	3 cut(s)	544, 1540, 1657
Bst6I	CTCTTC	2 cut(s)	312, 1562
BstAFI	CTTAAG	1 cut(s)	1901
BstAPI	GCANNNNNTGC	1 cut(s)	1103
BstBI	TTCGAA	2 cut(s)	547, 665
BstC8I	GCNNGC	1 cut(s)	1698
BstDEI	CTNAG	4 cut(s)	222, 354, 487, 783
BstDSI	CCRYGG	1 cut(s)	999
BstENI	CCTNNNNNAGG	1 cut(s)	1612
BstF5I	GGATG	2 cut(s)	1410, 1750
BstFNI	CGCG	1 cut(s)	1064
BstHHI	GCGC	3 cut(s)	75, 272, 1404
BstKTI	GATC	5 cut(s)	853, 1149, 1440, 1650, 1768
BstMAI	GTCTC	3 cut(s)	114, 765, 1592
BstMBI	GATC	5 cut(s)	850, 1146, 1437, 1647, 1765
BstMWI	GCNNNNNNNGC	3 cut(s)	1004, 1103, 1349
BstNI	CCWGG	3 cut(s)	267, 1212, 1431
BstNSI	RCATGY	4 cut(s)	521, 1192, 1715, 1989
BstSCI	CCNGG	4 cut(s)	83, 265, 1210, 1429
BstSFI	CTRYAG	1 cut(s)	1265
BstUI	CGCG	1 cut(s)	1064
BstV2I	GAAGAC	4 cut(s)	142, 220, 489, 1373
BstX2I	RGATCY	3 cut(s)	850, 1146, 1437
BstYI	RGATCY	3 cut(s)	850, 1146, 1437
BsuI	GTATCC	1 cut(s)	1399
BsuRI	GGCC	3 cut(s)	796, 998, 1976
BtgI	CCRYGG	1 cut(s)	999
BtsCI	GGATG	2 cut(s)	1410, 1750
BtsI	GCAGTG	2 cut(s)	1202, 1851
BtsIMutI	CAGTG	6 cut(s)	529, 791, 1202, 1653, 1851, 1984
Cac8I	GCNNGC	1 cut(s)	1698
CfoI	GCGC	3 cut(s)	75, 272, 1404
Cfr10I	RCCGGY	1 cut(s)	1303
Cfr13I	GGNCC	6 cut(s)	5, 23, 296, 592, 1720, 1844
CseI	GACGC	1 cut(s)	1070
Csp6I	GTAC	6 cut(s)	877, 945, 1032, 1272, 1452, 1912
CviQI	GTAC	6 cut(s)	877, 945, 1032, 1272, 1452, 1912
DdeI	CTNAG	4 cut(s)	222, 354, 487, 783
DpnI	GATC	5 cut(s)	852, 1148, 1439, 1649, 1767
DpnII	GATC	5 cut(s)	850, 1146, 1437, 1647, 1765
EaeI	YGGCCR	2 cut(s)	794, 1974
Eam1104I	CTCTTC	2 cut(s)	312, 1562
EarI	CTCTTC	2 cut(s)	312, 1562
Eco130I	CCWWGG	1 cut(s)	999
Eco31I	GGTCTC	1 cut(s)	1592
Eco47I	GGWCC	6 cut(s)	5, 23, 296, 592, 1720, 1844
Eco57I	CTGAAG	6 cut(s)	434, 501, 754, 824, 1099, 1488
Eco88I	CYCGRG	1 cut(s)	312
EcoNI	CCTNNNNNAGG	1 cut(s)	1612
EcoRI	GAATTC	1 cut(s)	579
EcoRII	CCWGG	3 cut(s)	265, 1210, 1429
EcoT14I	CCWWGG	1 cut(s)	999
EcoT22I	ATGCAT	3 cut(s)	632, 742, 1672
ErhI	CCWWGG	1 cut(s)	999
FalI	AAGNNNNNCTT	4 cut(s)	816, 848, 1061, 1093
FaqI	GGGAC	6 cut(s)	309, 578, 1275, 1421, 1564, 1886
FauI	CCCGC	1 cut(s)	1650
FauNDI	CATATG	4 cut(s)	304, 719, 1048, 1091
FblI	GTMKAC	1 cut(s)	751
Fnu4HI	GCNGC	1 cut(s)	1186
FokI	GGATG	2 cut(s)	1397, 1757
Fsp4HI	GCNGC	1 cut(s)	1186
FspBI	CTAG	6 cut(s)	131, 251, 747, 755, 924, 1544
FspI	TGCGCA	1 cut(s)	74
GlaI	GCGC	3 cut(s)	74, 271, 1403
GluI	GCNGC	1 cut(s)	1186
GsaI	CCCAGC	2 cut(s)	905, 1135
GsuI	CTGGAG	1 cut(s)	1452
HaeIII	GGCC	3 cut(s)	796, 998, 1976
HapII	CCGG	6 cut(s)	84, 470, 1286, 1304, 1496, 1718
HgaI	GACGC	1 cut(s)	1070
HhaI	GCGC	3 cut(s)	75, 272, 1404
Hin6I	GCGC	3 cut(s)	73, 270, 1402
HinP1I	GCGC	3 cut(s)	73, 270, 1402
HincII	GTYRAC	1 cut(s)	1154
HindII	GTYRAC	1 cut(s)	1154
HinfI	GANTC	6 cut(s)	107, 382, 503, 1043, 1139, 1604
HpaII	CCGG	6 cut(s)	84, 470, 1286, 1304, 1496, 1718
HphI	GGTGA	6 cut(s)	223, 542, 1193, 1226, 1310, 1601
Hpy166II	GTNNAC	2 cut(s)	752, 1154
Hpy188I	TCNGA	9 cut(s)	114, 355, 414, 606, 975, 1146, 1442, 1566, 1930
Hpy188III	TCNNGA	7 cut(s)	21, 314, 479, 1117, 1223, 1544, 1718
Hpy8I	GTNNAC	2 cut(s)	752, 1154
Hpy99I	CGWCG	1 cut(s)	1446
HpyAV	CCTTC	6 cut(s)	821, 921, 1470, 1792, 1857, 1935
HpyCH4III	ACNGT	3 cut(s)	544, 1540, 1657
HpyCH4IV	ACGT	2 cut(s)	1444, 1483
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1004, 1103, 1349
HpyF3I	CTNAG	4 cut(s)	222, 354, 487, 783
HpySE526I	ACGT	2 cut(s)	1444, 1483
HspAI	GCGC	3 cut(s)	73, 270, 1402
Kpn2I	TCCGGA	1 cut(s)	1717
KpnI	GGTACC	2 cut(s)	880, 1035
Kzo9I	GATC	5 cut(s)	850, 1146, 1437, 1647, 1765
LmnI	GCTCC	3 cut(s)	334, 1705, 1838
LweI	GCATC	9 cut(s)	70, 348, 385, 617, 658, 876, 1582, 1657, 1679
MaeI	CTAG	6 cut(s)	131, 251, 747, 755, 924, 1544
MaeII	ACGT	2 cut(s)	1444, 1483
MaeIII	GTNAC	6 cut(s)	307, 370, 495, 568, 1199, 1805
MalI	GATC	5 cut(s)	852, 1148, 1439, 1649, 1767
MboI	GATC	5 cut(s)	850, 1146, 1437, 1647, 1765
MflI	RGATCY	3 cut(s)	850, 1146, 1437
MlsI	TGGCCA	2 cut(s)	796, 1976
MluI	ACGCGT	1 cut(s)	1062
MluNI	TGGCCA	2 cut(s)	796, 1976
MlyI	GAGTC	4 cut(s)	116, 497, 1052, 1598
MmeI	TCCRAC	1 cut(s)	1465
Mox20I	TGGCCA	2 cut(s)	796, 1976
Mph1103I	ATGCAT	3 cut(s)	632, 742, 1672
MroI	TCCGGA	1 cut(s)	1717
MscI	TGGCCA	2 cut(s)	796, 1976
MseI	TTAA	6 cut(s)	153, 165, 623, 1335, 1893, 1902
MslI	CAYNNNNRTG	5 cut(s)	522, 625, 654, 1212, 1955
Msp20I	TGGCCA	2 cut(s)	796, 1976
MspCI	CTTAAG	1 cut(s)	1901
MspI	CCGG	6 cut(s)	84, 470, 1286, 1304, 1496, 1718
MspR9I	CCNGG	4 cut(s)	85, 267, 1212, 1431
Mva1269I	GAATGC	1 cut(s)	1354
MvaI	CCWGG	3 cut(s)	267, 1212, 1431
MvnI	CGCG	1 cut(s)	1064
MwoI	GCNNNNNNNGC	3 cut(s)	1004, 1103, 1349
NciI	CCSGG	1 cut(s)	85
NcoI	CCATGG	1 cut(s)	999
NdeI	CATATG	4 cut(s)	304, 719, 1048, 1091
NdeII	GATC	5 cut(s)	850, 1146, 1437, 1647, 1765
NmeAIII	GCCGAG	1 cut(s)	203
NmuCI	GTSAC	3 cut(s)	495, 1199, 1805
NsbI	TGCGCA	1 cut(s)	74
NsiI	ATGCAT	3 cut(s)	632, 742, 1672
NspI	RCATGY	4 cut(s)	521, 1192, 1715, 1989
NspV	TTCGAA	2 cut(s)	547, 665
OliI	CACNNNNGTG	1 cut(s)	1212
PaeR7I	CTCGAG	1 cut(s)	312
PciI	ACATGT	3 cut(s)	517, 1711, 1985
PctI	GAATGC	1 cut(s)	1354
PfeI	GAWTC	2 cut(s)	382, 1139
PflMI	CCANNNNNTGG	1 cut(s)	1211
PfoI	TCCNGGA	1 cut(s)	1429
PkrI	GCNGC	1 cut(s)	1187
PleI	GAGTC	4 cut(s)	115, 497, 1051, 1598
PpsI	GAGTC	4 cut(s)	115, 497, 1051, 1598
PscI	ACATGT	3 cut(s)	517, 1711, 1985
Psp1406I	AACGTT	1 cut(s)	1483
Psp6I	CCWGG	3 cut(s)	265, 1210, 1429
PspFI	CCCAGC	2 cut(s)	901, 1131
PspGI	CCWGG	3 cut(s)	265, 1210, 1429
PspPI	GGNCC	6 cut(s)	5, 23, 296, 592, 1720, 1844
PsrI	GAACNNNNNNTAC	2 cut(s)	911, 943
PsuI	RGATCY	3 cut(s)	850, 1146, 1437
RsaI	GTAC	6 cut(s)	878, 946, 1033, 1273, 1453, 1913
RsaNI	GTAC	6 cut(s)	877, 945, 1032, 1272, 1452, 1912
RseI	CAYNNNNRTG	5 cut(s)	522, 625, 654, 1212, 1955
SaqAI	TTAA	6 cut(s)	153, 165, 623, 1335, 1893, 1902
SatI	GCNGC	1 cut(s)	1186
Sau3AI	GATC	5 cut(s)	850, 1146, 1437, 1647, 1765
Sau96I	GGNCC	6 cut(s)	5, 23, 296, 592, 1720, 1844
ScaI	AGTACT	1 cut(s)	946
SchI	GAGTC	4 cut(s)	116, 497, 1052, 1598
ScrFI	CCNGG	4 cut(s)	85, 267, 1212, 1431
SfaNI	GCATC	9 cut(s)	70, 348, 385, 617, 658, 876, 1582, 1657, 1679
SfcI	CTRYAG	1 cut(s)	1265
Sfr274I	CTCGAG	1 cut(s)	312
SfuI	TTCGAA	2 cut(s)	547, 665
SinI	GGWCC	6 cut(s)	5, 23, 296, 592, 1720, 1844
SlaI	CTCGAG	1 cut(s)	312
SmiMI	CAYNNNNRTG	5 cut(s)	522, 625, 654, 1212, 1955
SmlI	CTYRAG	3 cut(s)	312, 1886, 1901
SmoI	CTYRAG	3 cut(s)	312, 1886, 1901
SpeI	ACTAGT	1 cut(s)	754
SsiI	CCGC	3 cut(s)	613, 1185, 1643
SspMI	CTAG	6 cut(s)	131, 251, 747, 755, 924, 1544
StyD4I	CCNGG	4 cut(s)	83, 265, 1210, 1429
StyI	CCWWGG	1 cut(s)	999
TaaI	ACNGT	3 cut(s)	544, 1540, 1657
TaiI	ACGT	2 cut(s)	1447, 1486
TaqI	TCGA	7 cut(s)	36, 192, 313, 437, 547, 665, 1041
TaqII	GACCGA	2 cut(s)	591, 1616
TatI	WGTACW	1 cut(s)	944
TauI	GCSGC	1 cut(s)	1188
TfiI	GAWTC	2 cut(s)	382, 1139
Tru1I	TTAA	6 cut(s)	153, 165, 623, 1335, 1893, 1902
Tru9I	TTAA	6 cut(s)	153, 165, 623, 1335, 1893, 1902
TscAI	CASTG	6 cut(s)	529, 791, 1202, 1660, 1858, 1984
TseFI	GTSAC	3 cut(s)	495, 1199, 1805
Tsp45I	GTSAC	3 cut(s)	495, 1199, 1805
TspGWI	ACGGA	2 cut(s)	44, 1139
TspRI	CASTG	6 cut(s)	529, 791, 1202, 1660, 1858, 1984
Van91I	CCANNNNNTGG	1 cut(s)	1211
Vha464I	CTTAAG	1 cut(s)	1901
VpaK11BI	GGWCC	6 cut(s)	5, 23, 296, 592, 1720, 1844
XagI	CCTNNNNNAGG	1 cut(s)	1612
XapI	RAATTY	4 cut(s)	200, 579, 1331, 1960
XbaI	TCTAGA	1 cut(s)	1543
XceI	RCATGY	4 cut(s)	521, 1192, 1715, 1989
XhoI	CTCGAG	1 cut(s)	312
XmiI	GTMKAC	1 cut(s)	751
XspI	CTAG	6 cut(s)	131, 251, 747, 755, 924, 1544
ZrmI	AGTACT	1 cut(s)	946
Zsp2I	ATGCAT	3 cut(s)	632, 742, 1672

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.