Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0018359

Physical Location & Seq

Genomic Coordinates Forward (+)

33305294 .. 33310399

Gene Structure 5106 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G023040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1959 bp

ATGCAGAGACGGGGAAGGCTCTTGGATGGTTTCCAGAATTCCATCAGCCTTAAGATTTTTTTGGGGTTTTTTTTTTATAAAAAAAATTATAATTTGATGGAGAAACTTGAACCCCATTTCGTGGTGAGGATGGGAGTCAGAAATATTGTTAAGCTATGGGTTAAAGCAATTAGCCGAGGCAAAGGTTTCGATGAGGAATTTGTAGAAGAAGCAAATGCTAAGGTCTTCACCTTTGGGTCATACAGGCTAGGAGTTCATAGCCCTGGTGCAGATATAGATACACTCTGCGTGGGACCGGCATATGTAACTCGAGAAGAGGGTTTCTTTGGGGACCTGTATAAGATTTTGTCTGAGATGCCAGAAGAAGTAACCGAGTTGAATCCTGTGCATGATGCTCATGTTCCAGTTCTGAAGTTCAAGTTCAATGGGGTTTCGATAGACCTTGTTTATGCAAGACTATCGCTTCCGGTTATTCCTGAAGACTTGGATTTGTCACAAGACTCAATCCTACAACATGTCAGTGCTGGTGATGAACAAACTGTTCGAAGCCTCAACGGTTGCAGGGTTACAGACCAAATTCTTAAGTTGGTCCCAAACATTCAGACTTTCCGCACAACATTAAGATGCATTAGGTTATGGGCAAAGCATCGTGGTGTTTATTCGAATGTTGCAGGGTTTCTTGGTGGTATAAACTGGGCATTGCTTGTGGCTTTCATTTGCCAACTTTACCCAAATGCATTGCCTAGTCTACTAGTGTCTCGGTTCTTCAGGGTCTATACTCAGTGGCGTTGGCCAAATCCAGTATTGCTCTGCCCTATCAAAGAAGGGCTTGGACTTCAGGTTTGGGATCCAAGAAGAAATGTAAGGGATAGGTACCATTTGATGCCTATAATTACTCCAGCTTATCCTTCCATGAACTCTAGCTACAATGTGTCGCGGAGTACTTTGCGTATCATGTTAGGAGAGTTTCAGAGGGGAAATGACATTTGTAAGGCCATGGAAGCAGAAAATAAGGATGTTGATTGGGGTACCCTTTTCGAGTCATATGCTTTCTTTGACGCGTATAAGAATTACTTGCAGATAGACACAAGTGCAGGAGGTGCTGATGACTTCAGGAAATGGAAAGGCTGGGTTGAGTCTCGGATCCGTCAACTAACACTCAAATGTCACCCACACCCTGGTGATTTTCCAGACAAGACCCTACCTTATCATAGCTCATTCTTCATGGGACTACAGCGTACCCAACAAGTTCCGGTCAAAGGAGGTGAGCCGGTTCATTTAGGGAGATGTGTTGATGAATTTAAGTGGGCTGTGAATGCTTATACTTCAAGGAAGACTGGAATGGACATTCGTGTATCCCATGTAAAGCGCAGGGACATCCCTAATTTTGTATTTCCTGGAGGGATCCGACCTTTGCGTACATCTAAAGCAACTTGGGAGAGAAGGCAACTTTCCCCTCAACCGGAGGCTTCAGGCGACATAGCTCAATGTCAGTCTAAAACAACTGTTCTAGATGGGACAGATGGAGGTCAGAAGAGAAAAAGGCAGGAGAATGGTGATGCAGAGACCGACTCCTCATCAAGGCACCCCAAGTCTGTGAGGTGCAACCCACCGATCAACACTGTTGTCGCCGATGCATCAATTTCTAAACAAGCAGAGAAGCTGTCACGGAAGCCACATGTTCCGGACCAAGTTTTGCCAAAAGAACTGGATGAGCTTGAAGATGATAGAGATCAAGTCAAAGACTTTGCTGGTGGGAACATGAAGGATAGTCACACAATGGGGGCAATAAAATCTAATAATGGAGCTGGTCCTTCCACTGCTTGTTCATATATTGGGGACTTGGAGGAACTTGAGTTTAATGAACTTAAGGTGCCGTACTATCAAACCCCTTCTGAACCTGTGGCGAAGCCAATCATCAGAGTCAATTTCATTACATTGGCCAGTGGAACATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004652 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008285 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0022414 GO:0031123 GO:0031124 GO:0031347 GO:0031348 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045824 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048441 GO:0048444 GO:0048446 GO:0048449 GO:0048451 GO:0048465 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0070013 GO:0070566 GO:0071704 GO:0080134 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901360 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

NTP_transf_2 PAP_RNA-bind PAP_central

Protein Analysis

652

Amino Acids

73.78

Weight (kDa)

8.79

Isoelectric Point (pI)

39.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAP_NTPase	PF20750	46 - 197	8.5e-53	Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2	PF01909	66 - 150	3.5e-11	Nucleotidyltransferase domain
PAP_central	PF04928	202 - 347	1.5e-63	Poly(A) polymerase central domain
PAP_RNA-bind	PF04926	351 - 388	2.8e-09	Poly(A) polymerase predicted RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0018359)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g24260
rosa_chinensis	RchiOBHm_Chr2g0120741
rosa_laevigata	RLG00000018576
rosa_multiflora	Rmu_sc0000319.1_g000010
rosa_rugosa	Rorug02G0230600
rosa_samantha	Rh2AG287300 Rh2BG298800 Rh2CG276700 Rh2DG312600
rosa_wichuraiana	Rw2G023040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	78, 90
Acc65I	GGTACC	2 cut(s)	873, 1028
AccB1I	GGYRCC	4 cut(s)	873, 1028, 1584, 1873
AccB7I	CCANNNNNTGG	2 cut(s)	121, 1178
AccI	GTMKAC	1 cut(s)	748
AccII	CGCG	2 cut(s)	937, 1061
AccIII	TCCGGA	1 cut(s)	1684
AciI	CCGC	2 cut(s)	610, 937
AclWI	GGATC	6 cut(s)	842, 855, 1138, 1151, 1399, 1412
AcoI	YGGCCR	2 cut(s)	791, 1941
AcsI	RAATTY	4 cut(s)	37, 197, 576, 1298
AcuI	CTGAAG	6 cut(s)	431, 498, 751, 821, 1096, 1455
AfaI	GTAC	6 cut(s)	875, 943, 1030, 1240, 1420, 1880
AfiI	CCNNNNNNNGG	5 cut(s)	121, 1178, 1259, 1462, 1581
AflII	CTTAAG	3 cut(s)	50, 581, 1868
AflIII	ACRYGT	4 cut(s)	514, 1059, 1678, 1952
AgsI	TTSAA	6 cut(s)	110, 379, 418, 424, 1329, 1721
AhlI	ACTAGT	1 cut(s)	751
AjnI	CCWGG	3 cut(s)	262, 1177, 1396
AleI	CACNNNNGTG	1 cut(s)	1179
AluBI	AGCT	8 cut(s)	154, 902, 924, 1215, 1484, 1663, 1717, 1808
AluI	AGCT	8 cut(s)	154, 902, 924, 1215, 1484, 1663, 1717, 1808
Alw26I	GTCTC	3 cut(s)	762, 1143, 1559
AlwI	GGATC	6 cut(s)	842, 855, 1138, 1151, 1399, 1412
Ama87I	CYCGRG	1 cut(s)	309
Aor13HI	TCCGGA	1 cut(s)	1684
AoxI	GGCC	3 cut(s)	791, 993, 1941
ApoI	RAATTY	4 cut(s)	37, 197, 576, 1298
ArsI	GACNNNNNNTTYG	2 cut(s)	825, 857
Asp718I	GGTACC	2 cut(s)	873, 1028
AspLEI	GCGC	1 cut(s)	1371
AspS9I	GGNCC	5 cut(s)	293, 331, 589, 1687, 1811
AsuHPI	GGTGA	7 cut(s)	136, 220, 539, 1160, 1193, 1277, 1568
AsuII	TTCGAA	2 cut(s)	544, 662
AvaI	CYCGRG	1 cut(s)	309
AvaII	GGWCC	5 cut(s)	293, 331, 589, 1687, 1811
BalI	TGGCCA	2 cut(s)	793, 1943
BamHI	GGATCC	3 cut(s)	847, 1143, 1404
BanI	GGYRCC	4 cut(s)	873, 1028, 1584, 1873
BbsI	GAAGAC	3 cut(s)	217, 486, 1340
BccI	CCATC	6 cut(s)	20, 50, 91, 124, 1508, 1517
BceAI	ACGGC	1 cut(s)	1861
BcgI	CGANNNNNNTGC	6 cut(s)	169, 203, 441, 475, 1028, 1062
BciT130I	CCWGG	3 cut(s)	264, 1179, 1398
BciVI	GTATCC	1 cut(s)	1366
BcoDI	GTCTC	3 cut(s)	762, 1143, 1559
BcuI	ACTAGT	1 cut(s)	751
BfaI	CTAG	5 cut(s)	248, 744, 752, 921, 1511
BfmI	CTRYAG	1 cut(s)	1232
BfrI	CTTAAG	3 cut(s)	50, 581, 1868
BfuI	GTATCC	1 cut(s)	1366
BmcAI	AGTACT	1 cut(s)	943
Bme1390I	CCNGG	3 cut(s)	264, 1179, 1398
Bme18I	GGWCC	5 cut(s)	293, 331, 589, 1687, 1811
BmeT110I	CYCGRG	1 cut(s)	309
BmgT120I	GGNCC	5 cut(s)	293, 331, 589, 1687, 1811
BmrFI	CCNGG	3 cut(s)	264, 1179, 1398
BmrI	ACTGGG	1 cut(s)	703
BmsI	GCATC	8 cut(s)	345, 382, 614, 655, 873, 1549, 1624, 1646
BmuI	ACTGGG	1 cut(s)	703
BpiI	GAAGAC	3 cut(s)	217, 486, 1340
BplI	GAGNNNNNCTC	2 cut(s)	1556, 1588
BpmI	CTGGAG	2 cut(s)	882, 1419
Bpu10I	CCTNAGC	1 cut(s)	219
Bpu14I	TTCGAA	2 cut(s)	544, 662
BpuEI	CTTGAG	1 cut(s)	1874
BsaBI	GATNNNNATC	1 cut(s)	1731
BsaI	GGTCTC	1 cut(s)	1559
BsaJI	CCNNGG	4 cut(s)	175, 262, 996, 1177
BsaWI	WCCGGW	4 cut(s)	466, 1252, 1462, 1684
Bsc4I	CCNNNNNNNGG	5 cut(s)	121, 1178, 1259, 1462, 1581
Bse118I	RCCGGY	2 cut(s)	295, 1270
Bse1I	ACTGG	6 cut(s)	404, 698, 800, 1342, 1713, 1944
Bse3DI	GCAATG	2 cut(s)	698, 737
Bse8I	GATNNNNATC	1 cut(s)	1731
BseAI	TCCGGA	1 cut(s)	1684
BseBI	CCWGG	3 cut(s)	264, 1179, 1398
BseDI	CCNNGG	4 cut(s)	175, 262, 996, 1177
BseGI	GGATG	5 cut(s)	31, 135, 1021, 1377, 1717
BseJI	GATNNNNATC	1 cut(s)	1731
BseLI	CCNNNNNNNGG	5 cut(s)	121, 1178, 1259, 1462, 1581
BseMI	GCAATG	2 cut(s)	698, 737
BseMII	CTCAG	2 cut(s)	342, 794
BseNI	ACTGG	6 cut(s)	404, 698, 800, 1342, 1713, 1944
BseRI	GAGGAG	1 cut(s)	1564
BseYI	CCCAGC	1 cut(s)	1128
BsgI	GTGCAG	2 cut(s)	288, 1113
Bsh1236I	CGCG	2 cut(s)	937, 1061
BshFI	GGCC	3 cut(s)	793, 995, 1943
BshNI	GGYRCC	4 cut(s)	873, 1028, 1584, 1873
BsiHKCI	CYCGRG	1 cut(s)	309
BsiSI	CCGG	6 cut(s)	296, 467, 1253, 1271, 1463, 1685
BslFI	GGGAC	7 cut(s)	306, 344, 575, 1242, 1388, 1531, 1853
BslI	CCNNNNNNNGG	5 cut(s)	121, 1178, 1259, 1462, 1581
BsmAI	GTCTC	3 cut(s)	762, 1143, 1559
BsmFI	GGGAC	7 cut(s)	306, 344, 575, 1242, 1388, 1531, 1853
BsmI	GAATGC	1 cut(s)	1321
BsnI	GGCC	3 cut(s)	793, 995, 1943
Bso31I	GGTCTC	1 cut(s)	1559
BsoBI	CYCGRG	1 cut(s)	309
Bsp119I	TTCGAA	2 cut(s)	544, 662
Bsp13I	TCCGGA	1 cut(s)	1684
Bsp143I	GATC	5 cut(s)	847, 1143, 1404, 1614, 1732
Bsp19I	CCATGG	1 cut(s)	996
BspACI	CCGC	2 cut(s)	610, 937
BspANI	GGCC	3 cut(s)	793, 995, 1943
BspCNI	CTCAG	2 cut(s)	343, 793
BspEI	TCCGGA	1 cut(s)	1684
BspFNI	CGCG	2 cut(s)	937, 1061
BspPI	GGATC	6 cut(s)	842, 855, 1138, 1151, 1399, 1412
BspT104I	TTCGAA	2 cut(s)	544, 662
BspT107I	GGYRCC	4 cut(s)	873, 1028, 1584, 1873
BspTI	CTTAAG	3 cut(s)	50, 581, 1868
BspTNI	GGTCTC	1 cut(s)	1559
BsrDI	GCAATG	2 cut(s)	698, 737
BsrFI	RCCGGY	2 cut(s)	295, 1270
BsrI	ACTGG	6 cut(s)	404, 698, 800, 1342, 1713, 1944
BssAI	RCCGGY	2 cut(s)	295, 1270
BssECI	CCNNGG	4 cut(s)	175, 262, 996, 1177
BssMI	GATC	5 cut(s)	847, 1143, 1404, 1614, 1732
BssT1I	CCWWGG	1 cut(s)	996
Bst2UI	CCWGG	3 cut(s)	264, 1179, 1398
Bst4CI	ACNGT	4 cut(s)	541, 557, 1507, 1624
Bst6I	CTCTTC	2 cut(s)	309, 1529
BstAFI	CTTAAG	3 cut(s)	50, 581, 1868
BstAPI	GCANNNNNTGC	1 cut(s)	1100
BstBI	TTCGAA	2 cut(s)	544, 662
BstDEI	CTNAG	3 cut(s)	219, 351, 780
BstDSI	CCRYGG	1 cut(s)	996
BstENI	CCTNNNNNAGG	1 cut(s)	1579
BstF5I	GGATG	5 cut(s)	31, 135, 1021, 1377, 1717
BstFNI	CGCG	2 cut(s)	937, 1061
BstHHI	GCGC	1 cut(s)	1371
BstKTI	GATC	5 cut(s)	850, 1146, 1407, 1617, 1735
BstMAI	GTCTC	3 cut(s)	762, 1143, 1559
BstMBI	GATC	5 cut(s)	847, 1143, 1404, 1614, 1732
BstMWI	GCNNNNNNNGC	3 cut(s)	1001, 1100, 1316
BstNI	CCWGG	3 cut(s)	264, 1179, 1398
BstNSI	RCATGY	3 cut(s)	518, 1682, 1956
BstSCI	CCNGG	3 cut(s)	262, 1177, 1396
BstSFI	CTRYAG	1 cut(s)	1232
BstUI	CGCG	2 cut(s)	937, 1061
BstV2I	GAAGAC	3 cut(s)	217, 486, 1340
BstX2I	RGATCY	3 cut(s)	847, 1143, 1404
BstYI	RGATCY	3 cut(s)	847, 1143, 1404
BsuI	GTATCC	1 cut(s)	1366
BsuRI	GGCC	3 cut(s)	793, 995, 1943
BtgI	CCRYGG	1 cut(s)	996
BtsCI	GGATG	5 cut(s)	31, 135, 1021, 1377, 1717
BtsI	GCAGTG	1 cut(s)	1818
BtsIMutI	CAGTG	5 cut(s)	526, 788, 1620, 1818, 1951
CfoI	GCGC	1 cut(s)	1371
Cfr10I	RCCGGY	2 cut(s)	295, 1270
Cfr13I	GGNCC	5 cut(s)	293, 331, 589, 1687, 1811
CseI	GACGC	1 cut(s)	1067
Csp6I	GTAC	6 cut(s)	874, 942, 1029, 1239, 1419, 1879
CviQI	GTAC	6 cut(s)	874, 942, 1029, 1239, 1419, 1879
DdeI	CTNAG	3 cut(s)	219, 351, 780
DpnI	GATC	5 cut(s)	849, 1145, 1406, 1616, 1734
DpnII	GATC	5 cut(s)	847, 1143, 1404, 1614, 1732
EaeI	YGGCCR	2 cut(s)	791, 1941
Eam1104I	CTCTTC	2 cut(s)	309, 1529
EarI	CTCTTC	2 cut(s)	309, 1529
Eco130I	CCWWGG	1 cut(s)	996
Eco31I	GGTCTC	1 cut(s)	1559
Eco47I	GGWCC	5 cut(s)	293, 331, 589, 1687, 1811
Eco57I	CTGAAG	6 cut(s)	431, 498, 751, 821, 1096, 1455
Eco88I	CYCGRG	1 cut(s)	309
EcoNI	CCTNNNNNAGG	1 cut(s)	1579
EcoO109I	RGGNCCY	1 cut(s)	331
EcoRI	GAATTC	1 cut(s)	37
EcoRII	CCWGG	3 cut(s)	262, 1177, 1396
EcoT14I	CCWWGG	1 cut(s)	996
EcoT22I	ATGCAT	3 cut(s)	629, 739, 1639
ErhI	CCWWGG	1 cut(s)	996
FalI	AAGNNNNNCTT	4 cut(s)	813, 845, 1058, 1090
FaqI	GGGAC	7 cut(s)	306, 344, 575, 1242, 1388, 1531, 1853
FauNDI	CATATG	2 cut(s)	301, 1045
FblI	GTMKAC	1 cut(s)	748
FokI	GGATG	5 cut(s)	38, 142, 1028, 1364, 1724
FspBI	CTAG	5 cut(s)	248, 744, 752, 921, 1511
GlaI	GCGC	1 cut(s)	1370
GsaI	CCCAGC	1 cut(s)	1132
GsuI	CTGGAG	2 cut(s)	882, 1419
HaeIII	GGCC	3 cut(s)	793, 995, 1943
HapII	CCGG	6 cut(s)	296, 467, 1253, 1271, 1463, 1685
HgaI	GACGC	1 cut(s)	1067
HhaI	GCGC	1 cut(s)	1371
Hin6I	GCGC	1 cut(s)	1369
HinP1I	GCGC	1 cut(s)	1369
HincII	GTYRAC	1 cut(s)	1151
HindII	GTYRAC	1 cut(s)	1151
HinfI	GANTC	7 cut(s)	135, 379, 500, 1040, 1136, 1571, 1923
HpaII	CCGG	6 cut(s)	296, 467, 1253, 1271, 1463, 1685
HphI	GGTGA	7 cut(s)	136, 220, 539, 1160, 1193, 1277, 1568
Hpy166II	GTNNAC	2 cut(s)	749, 1151
Hpy188III	TCNNGA	7 cut(s)	34, 311, 476, 1114, 1190, 1511, 1685
Hpy8I	GTNNAC	2 cut(s)	749, 1151
HpyAV	CCTTC	7 cut(s)	9, 818, 918, 1437, 1759, 1824, 1902
HpyCH4III	ACNGT	4 cut(s)	541, 557, 1507, 1624
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1001, 1100, 1316
HpyF3I	CTNAG	3 cut(s)	219, 351, 780
HspAI	GCGC	1 cut(s)	1369
Kpn2I	TCCGGA	1 cut(s)	1684
KpnI	GGTACC	2 cut(s)	877, 1032
Kzo9I	GATC	5 cut(s)	847, 1143, 1404, 1614, 1732
LmnI	GCTCC	1 cut(s)	1805
LweI	GCATC	8 cut(s)	345, 382, 614, 655, 873, 1549, 1624, 1646
MaeI	CTAG	5 cut(s)	248, 744, 752, 921, 1511
MaeIII	GTNAC	7 cut(s)	304, 367, 492, 565, 1166, 1665, 1772
MalI	GATC	5 cut(s)	849, 1145, 1406, 1616, 1734
MboI	GATC	5 cut(s)	847, 1143, 1404, 1614, 1732
MflI	RGATCY	3 cut(s)	847, 1143, 1404
MlsI	TGGCCA	2 cut(s)	793, 1943
MluI	ACGCGT	1 cut(s)	1059
MluNI	TGGCCA	2 cut(s)	793, 1943
MlyI	GAGTC	6 cut(s)	144, 494, 1049, 1145, 1565, 1932
MmeI	TCCRAC	1 cut(s)	1432
Mox20I	TGGCCA	2 cut(s)	793, 1943
Mph1103I	ATGCAT	3 cut(s)	629, 739, 1639
MroI	TCCGGA	1 cut(s)	1684
MscI	TGGCCA	2 cut(s)	793, 1943
MseI	TTAA	8 cut(s)	51, 150, 162, 582, 620, 1302, 1860, 1869
MslI	CAYNNNNRTG	5 cut(s)	519, 622, 651, 1162, 1179
Msp20I	TGGCCA	2 cut(s)	793, 1943
MspCI	CTTAAG	3 cut(s)	50, 581, 1868
MspI	CCGG	6 cut(s)	296, 467, 1253, 1271, 1463, 1685
MspR9I	CCNGG	3 cut(s)	264, 1179, 1398
Mva1269I	GAATGC	1 cut(s)	1321
MvaI	CCWGG	3 cut(s)	264, 1179, 1398
MvnI	CGCG	2 cut(s)	937, 1061
MwoI	GCNNNNNNNGC	3 cut(s)	1001, 1100, 1316
NcoI	CCATGG	1 cut(s)	996
NdeI	CATATG	2 cut(s)	301, 1045
NdeII	GATC	5 cut(s)	847, 1143, 1404, 1614, 1732
NmeAIII	GCCGAG	1 cut(s)	200
NmuCI	GTSAC	4 cut(s)	492, 1166, 1665, 1772
NsiI	ATGCAT	3 cut(s)	629, 739, 1639
NspI	RCATGY	3 cut(s)	518, 1682, 1956
NspV	TTCGAA	2 cut(s)	544, 662
OliI	CACNNNNGTG	1 cut(s)	1179
PaeR7I	CTCGAG	1 cut(s)	309
PciI	ACATGT	3 cut(s)	514, 1678, 1952
PctI	GAATGC	1 cut(s)	1321
PfeI	GAWTC	1 cut(s)	379
PflMI	CCANNNNNTGG	2 cut(s)	121, 1178
PfoI	TCCNGGA	1 cut(s)	1396
PleI	GAGTC	6 cut(s)	143, 494, 1048, 1144, 1565, 1931
PpsI	GAGTC	6 cut(s)	143, 494, 1048, 1144, 1565, 1931
PpuMI	RGGWCCY	1 cut(s)	331
PscI	ACATGT	3 cut(s)	514, 1678, 1952
PsiI	TTATAA	2 cut(s)	78, 90
Psp5II	RGGWCCY	1 cut(s)	331
Psp6I	CCWGG	3 cut(s)	262, 1177, 1396
PspFI	CCCAGC	1 cut(s)	1128
PspGI	CCWGG	3 cut(s)	262, 1177, 1396
PspPI	GGNCC	5 cut(s)	293, 331, 589, 1687, 1811
PspPPI	RGGWCCY	1 cut(s)	331
PsrI	GAACNNNNNNTAC	2 cut(s)	908, 940
PsuI	RGATCY	3 cut(s)	847, 1143, 1404
RsaI	GTAC	6 cut(s)	875, 943, 1030, 1240, 1420, 1880
RsaNI	GTAC	6 cut(s)	874, 942, 1029, 1239, 1419, 1879
RseI	CAYNNNNRTG	5 cut(s)	519, 622, 651, 1162, 1179
SaqAI	TTAA	8 cut(s)	51, 150, 162, 582, 620, 1302, 1860, 1869
Sau3AI	GATC	5 cut(s)	847, 1143, 1404, 1614, 1732
Sau96I	GGNCC	5 cut(s)	293, 331, 589, 1687, 1811
ScaI	AGTACT	1 cut(s)	943
SchI	GAGTC	6 cut(s)	144, 494, 1049, 1145, 1565, 1932
ScrFI	CCNGG	3 cut(s)	264, 1179, 1398
SfaNI	GCATC	8 cut(s)	345, 382, 614, 655, 873, 1549, 1624, 1646
SfcI	CTRYAG	1 cut(s)	1232
Sfr274I	CTCGAG	1 cut(s)	309
SfuI	TTCGAA	2 cut(s)	544, 662
SinI	GGWCC	5 cut(s)	293, 331, 589, 1687, 1811
SlaI	CTCGAG	1 cut(s)	309
SmiMI	CAYNNNNRTG	5 cut(s)	519, 622, 651, 1162, 1179
SmlI	CTYRAG	5 cut(s)	50, 309, 581, 1853, 1868
SmoI	CTYRAG	5 cut(s)	50, 309, 581, 1853, 1868
SpeI	ACTAGT	1 cut(s)	751
SsiI	CCGC	2 cut(s)	610, 937
SspI	AATATT	1 cut(s)	145
SspMI	CTAG	5 cut(s)	248, 744, 752, 921, 1511
StyD4I	CCNGG	3 cut(s)	262, 1177, 1396
StyI	CCWWGG	1 cut(s)	996
TaaI	ACNGT	4 cut(s)	541, 557, 1507, 1624
TaqI	TCGA	6 cut(s)	189, 310, 434, 544, 662, 1038
TaqII	GACCGA	1 cut(s)	1583
TatI	WGTACW	1 cut(s)	941
TfiI	GAWTC	1 cut(s)	379
Tru1I	TTAA	8 cut(s)	51, 150, 162, 582, 620, 1302, 1860, 1869
Tru9I	TTAA	8 cut(s)	51, 150, 162, 582, 620, 1302, 1860, 1869
TscAI	CASTG	5 cut(s)	526, 788, 1627, 1825, 1951
TseFI	GTSAC	4 cut(s)	492, 1166, 1665, 1772
Tsp45I	GTSAC	4 cut(s)	492, 1166, 1665, 1772
TspGWI	ACGGA	2 cut(s)	1136, 1684
TspRI	CASTG	5 cut(s)	526, 788, 1627, 1825, 1951
Van91I	CCANNNNNTGG	2 cut(s)	121, 1178
Vha464I	CTTAAG	3 cut(s)	50, 581, 1868
VpaK11BI	GGWCC	5 cut(s)	293, 331, 589, 1687, 1811
XagI	CCTNNNNNAGG	1 cut(s)	1579
XapI	RAATTY	4 cut(s)	37, 197, 576, 1298
XbaI	TCTAGA	1 cut(s)	1510
XceI	RCATGY	3 cut(s)	518, 1682, 1956
XhoI	CTCGAG	1 cut(s)	309
XmiI	GTMKAC	1 cut(s)	748
XspI	CTAG	5 cut(s)	248, 744, 752, 921, 1511
ZrmI	AGTACT	1 cut(s)	943
Zsp2I	ATGCAT	3 cut(s)	629, 739, 1639

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.