RchiOBHm_Chr2g0150161

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017782
Physical Location & Seq
Forward (+)
67904307 .. 67906300
1994 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
ATGGCTGGACGACTGGGGCTACTTGCTTGGGTGATCTTCATCCTGTCCGCTTCAGCAATCGTTAACAACAACTCAGTCCTTCTTCAAGCTGCAGCTCTTTCCAATGACATCGGCGCAAACTATCATATCCATGACAGTACTGGTGCTAGCGGTAATTTTTCCAAGGTGTTCGAGATTTTGGTCAAATGTGGCATAAGAAATTTGAGGGTCTTTTCAGATGAGATCAATGCCCCATATCTAGACTACTATGGAGCCTCCACTGGCATTGCTCTCACTGTTAGCATATCAAATTCTGATGTGCAAAGGTTCGCTGCAAGCTTTGAAGACTTAACCAAGTGGCACCAAGATGTTTTGTATTCCTTACATATCACCTTTGATGTCAAGTACATCACTGTTGGTAACGAAGCAATCCCCGGTCCCTCTACCGATGATGTTCTGACCGTAATGAAGTCTCTACAACTGCTGAACAATTATAGCTCTTTTGGGGACATCAAGGTGACAACCTCTGTGGGATCAAGTGTTTTGGGGGATTGGTATCTACCGTCTAGTGGGGAGTTCAAACCAAATGCTAGCAGAGCCATGACTGACATACTTGGATTTCTATCAGCGTCTCAAGCACCTCTCATGATAAATGTGTACCCCTACTATGCATATGCAGCTGATCCGGCCAATGTACCGCTAGATTATGCACTCTTAAACGCCACCTACCCTATGGTTCATGATGGCTCATTGGAATACAGTAACTTGTTTGATGCAATGGTGGACTCCTTTTACTCTGCCATGGAGAAAGTTGACTACTCAGACGTGAGCATTGTGGTATCCGAGAGTGGGTGGCCTCGTGATGGGAATTACAACTTCACCACACCGGAACTTGCAGCTACATACAACAGGAATTTCCTGCTACGCATCAGCTCAAAGGTGGGAACACCCAAGAGACCTGATGCTTCTGTTAAGGGATACATATATGAGCTGTTTGATGGTAAAAGCCCTCACTTTGGACTCTTCGATAAGACCTTTGAACCTATCTATCCTCTGTTCCAGTGTTCCTTCTAG

Protein Analysis

350

Amino Acids

38.5

Weight (kDa)

4.78

Isoelectric Point (pI)

30.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 64 - 344 2.3e-50 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017782)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0150161
rosa_laevigata RLG00000020472
rosa_multiflora Rmu_co8401597.1_g000001 Rmu_sc0015510.1_g000001 Rmu_ssc0000020.1_g000012
rosa_roxburghii Rroxscaffold_2G00097390
rosa_rugosa Rorug02G0419400
rosa_samantha Rh2AG478300 Rh2BG490900 Rh2CG464500 Rh2DG499700
rosa_wichuraiana Rw2G039050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 339
AciI CCGC 3 cut(s) 48, 150, 677
AclWI GGATC 2 cut(s) 520, 656
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 3 cut(s) 199, 289, 892
AcuI CTGAAG 1 cut(s) 36
AfaI GTAC 4 cut(s) 139, 386, 638, 675
AfiI CCNNNNNNNGG 4 cut(s) 548, 828, 842, 995
AgsI TTSAA 4 cut(s) 86, 323, 559, 1019
AjiI CACGTC 1 cut(s) 805
AluBI AGCT 8 cut(s) 89, 95, 318, 477, 659, 878, 912, 970
AluI AGCT 8 cut(s) 89, 95, 318, 477, 659, 878, 912, 970
Alw26I GTCTC 3 cut(s) 456, 615, 928
AlwI GGATC 2 cut(s) 520, 656
AoxI GGCC 2 cut(s) 666, 833
ApeKI GCWGC 5 cut(s) 89, 92, 311, 656, 875
ApoI RAATTY 3 cut(s) 199, 289, 892
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 416
AsuC2I CCSGG 1 cut(s) 414
AsuHPI GGTGA 4 cut(s) 43, 361, 508, 850
AsuNHI GCTAGC 2 cut(s) 146, 569
AvaII GGWCC 1 cut(s) 416
BanI GGYRCC 1 cut(s) 339
BauI CACGAG 1 cut(s) 837
BbsI GAAGAC 1 cut(s) 330
BbvI GCAGC 5 cut(s) 76, 104, 298, 668, 887
BccI CCATC 3 cut(s) 716, 836, 971
BciVI GTATCC 2 cut(s) 829, 950
BcnI CCSGG 1 cut(s) 414
BcoDI GTCTC 3 cut(s) 456, 615, 928
BfaI CTAG 6 cut(s) 147, 239, 546, 570, 680, 1051
BfmI CTRYAG 1 cut(s) 90
BfuI GTATCC 2 cut(s) 829, 950
BisI GCNGC 5 cut(s) 90, 93, 312, 657, 876
BlsI GCNGC 5 cut(s) 91, 94, 313, 658, 877
BmcAI AGTACT 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 414
Bme18I GGWCC 1 cut(s) 416
BmgBI CACGTC 1 cut(s) 805
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 3 cut(s) 253, 341, 418
BmrFI CCNGG 1 cut(s) 414
BmrI ACTGGG 1 cut(s) 23
BmsI GCATC 3 cut(s) 742, 915, 931
BmtI GCTAGC 2 cut(s) 150, 573
BmuI ACTGGG 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 597
BpuMI CCSGG 1 cut(s) 414
BsaBI GATNNNNATC 3 cut(s) 38, 534, 601
BsaI GGTCTC 1 cut(s) 928
BsaJI CCNNGG 3 cut(s) 162, 412, 780
BsaWI WCCGGW 1 cut(s) 865
Bsc4I CCNNNNNNNGG 4 cut(s) 548, 828, 842, 995
Bse1I ACTGG 4 cut(s) 18, 145, 265, 1039
Bse3DI GCAATG 2 cut(s) 264, 762
Bse8I GATNNNNATC 3 cut(s) 38, 534, 601
BseDI CCNNGG 3 cut(s) 162, 412, 780
BseGI GGATG 1 cut(s) 39
BseJI GATNNNNATC 3 cut(s) 38, 534, 601
BseLI CCNNNNNNNGG 4 cut(s) 548, 828, 842, 995
BseMI GCAATG 2 cut(s) 264, 762
BseMII CTCAG 2 cut(s) 87, 813
BseNI ACTGG 4 cut(s) 18, 145, 265, 1039
BseXI GCAGC 5 cut(s) 76, 104, 298, 668, 887
BshFI GGCC 2 cut(s) 668, 835
BshNI GGYRCC 1 cut(s) 339
BsiSI CCGG 3 cut(s) 414, 665, 866
BslFI GGGAC 2 cut(s) 402, 500
BslI CCNNNNNNNGG 4 cut(s) 548, 828, 842, 995
BsmAI GTCTC 3 cut(s) 456, 615, 928
BsmBI CGTCTC 1 cut(s) 615
BsmFI GGGAC 2 cut(s) 402, 500
BsnI GGCC 2 cut(s) 668, 835
Bso31I GGTCTC 1 cut(s) 928
Bsp143I GATC 4 cut(s) 33, 222, 512, 661
Bsp19I CCATGG 1 cut(s) 780
BspACI CCGC 3 cut(s) 48, 150, 677
BspANI GGCC 2 cut(s) 668, 835
BspCNI CTCAG 2 cut(s) 86, 812
BspHI TCATGA 2 cut(s) 624, 718
BspLI GGNNCC 3 cut(s) 253, 341, 418
BspMAI CTGCAG 1 cut(s) 94
BspOI GCTAGC 2 cut(s) 150, 573
BspPI GGATC 2 cut(s) 520, 656
BspT107I GGYRCC 1 cut(s) 339
BspTNI GGTCTC 1 cut(s) 928
BsrDI GCAATG 2 cut(s) 264, 762
BsrI ACTGG 4 cut(s) 18, 145, 265, 1039
BssECI CCNNGG 3 cut(s) 162, 412, 780
BssMI GATC 4 cut(s) 33, 222, 512, 661
BssSI CACGAG 1 cut(s) 837
BssT1I CCWWGG 2 cut(s) 162, 780
Bst2BI CACGAG 1 cut(s) 837
Bst4CI ACNGT 6 cut(s) 137, 277, 394, 442, 543, 740
Bst6I CTCTTC 1 cut(s) 1007
BstC8I GCNNGC 3 cut(s) 148, 316, 571
BstDEI CTNAG 2 cut(s) 73, 799
BstDSI CCRYGG 1 cut(s) 780
BstF5I GGATG 1 cut(s) 39
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 4 cut(s) 36, 225, 515, 664
BstMAI GTCTC 3 cut(s) 456, 615, 928
BstMBI GATC 4 cut(s) 33, 222, 512, 661
BstMWI GCNNNNNNNGC 4 cut(s) 575, 614, 656, 665
BstSCI CCNGG 1 cut(s) 412
BstSFI CTRYAG 1 cut(s) 90
BstV1I GCAGC 5 cut(s) 76, 104, 298, 668, 887
BstV2I GAAGAC 1 cut(s) 330
BsuI GTATCC 2 cut(s) 829, 950
BsuRI GGCC 2 cut(s) 668, 835
BtgI CCRYGG 1 cut(s) 780
BtrI CACGTC 1 cut(s) 805
BtsCI GGATG 1 cut(s) 39
BtsIMutI CAGTG 4 cut(s) 258, 273, 390, 1046
Cac8I GCNNGC 3 cut(s) 148, 316, 571
CciI TCATGA 2 cut(s) 624, 718
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 416
CseI GACGC 1 cut(s) 597
Csp6I GTAC 4 cut(s) 138, 385, 637, 674
CspCI CAANNNNNGTGG 4 cut(s) 247, 282, 489, 524
CviAII CATG 5 cut(s) 131, 580, 625, 719, 781
CviQI GTAC 4 cut(s) 138, 385, 637, 674
DdeI CTNAG 2 cut(s) 73, 799
DpnI GATC 4 cut(s) 35, 224, 514, 663
DpnII GATC 4 cut(s) 33, 222, 512, 661
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 1 cut(s) 1007
EarI CTCTTC 1 cut(s) 1007
Eco130I CCWWGG 2 cut(s) 162, 780
Eco31I GGTCTC 1 cut(s) 928
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 36
EcoT14I CCWWGG 2 cut(s) 162, 780
EcoT22I ATGCAT 1 cut(s) 652
ErhI CCWWGG 2 cut(s) 162, 780
Esp3I CGTCTC 1 cut(s) 615
FaeI CATG 5 cut(s) 134, 583, 628, 722, 784
FaqI GGGAC 2 cut(s) 402, 500
FatI CATG 5 cut(s) 130, 579, 624, 718, 780
FauNDI CATATG 1 cut(s) 652
Fnu4HI GCNGC 5 cut(s) 90, 93, 312, 657, 876
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 5 cut(s) 90, 93, 312, 657, 876
FspBI CTAG 6 cut(s) 147, 239, 546, 570, 680, 1051
GlaI GCGC 1 cut(s) 115
GluI GCNGC 5 cut(s) 90, 93, 312, 657, 876
HaeIII GGCC 2 cut(s) 668, 835
HapII CCGG 3 cut(s) 414, 665, 866
HgaI GACGC 1 cut(s) 597
HhaI GCGC 1 cut(s) 116
Hin1II CATG 5 cut(s) 134, 583, 628, 722, 784
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 2 cut(s) 64, 793
HindII GTYRAC 2 cut(s) 64, 793
HindIII AAGCTT 1 cut(s) 316
HinfI GANTC 2 cut(s) 764, 999
HpaI GTTAAC 1 cut(s) 64
HpaII CCGG 3 cut(s) 414, 665, 866
HphI GGTGA 4 cut(s) 43, 361, 508, 850
Hpy166II GTNNAC 4 cut(s) 64, 637, 763, 793
Hpy188I TCNGA 5 cut(s) 217, 295, 438, 802, 823
Hpy188III TCNNGA 5 cut(s) 172, 239, 625, 719, 839
Hpy8I GTNNAC 4 cut(s) 64, 637, 763, 793
HpyAV CCTTC 1 cut(s) 89
HpyCH4III ACNGT 6 cut(s) 137, 277, 394, 442, 543, 740
HpyCH4IV ACGT 1 cut(s) 804
HpyCH4V TGCA 8 cut(s) 92, 301, 314, 650, 656, 689, 755, 875
HpyF10VI GCNNNNNNNGC 4 cut(s) 575, 614, 656, 665
HpyF3I CTNAG 2 cut(s) 73, 799
HpySE526I ACGT 1 cut(s) 804
Hsp92II CATG 5 cut(s) 134, 583, 628, 722, 784
HspAI GCGC 1 cut(s) 114
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 4 cut(s) 33, 222, 512, 661
LmnI GCTCC 1 cut(s) 251
LpnPI CCDG 9 cut(s) 56, 126, 246, 427, 678, 874, 879, 911, 951
Lsp1109I GCAGC 5 cut(s) 76, 104, 298, 668, 887
LweI GCATC 3 cut(s) 742, 915, 931
MaeI CTAG 6 cut(s) 147, 239, 546, 570, 680, 1051
MaeII ACGT 1 cut(s) 804
MaeIII GTNAC 3 cut(s) 398, 496, 740
MalI GATC 4 cut(s) 35, 224, 514, 663
MboI GATC 4 cut(s) 33, 222, 512, 661
MboII GAAGA 4 cut(s) 28, 74, 335, 994
MluCI AATT 6 cut(s) 154, 199, 289, 469, 847, 892
MlyI GAGTC 2 cut(s) 758, 993
MnlI CCTC 8 cut(s) 198, 265, 430, 514, 630, 846, 999, 1041
Mph1103I ATGCAT 1 cut(s) 652
MseI TTAA 4 cut(s) 63, 329, 695, 951
MslI CAYNNNNRTG 3 cut(s) 129, 345, 494
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 3 cut(s) 414, 665, 866
MspR9I CCNGG 1 cut(s) 414
MwoI GCNNNNNNNGC 4 cut(s) 575, 614, 656, 665
NciI CCSGG 1 cut(s) 414
NcoI CCATGG 1 cut(s) 780
NdeI CATATG 1 cut(s) 652
NdeII GATC 4 cut(s) 33, 222, 512, 661
NheI GCTAGC 2 cut(s) 146, 569
NlaIII CATG 5 cut(s) 134, 583, 628, 722, 784
NlaIV GGNNCC 3 cut(s) 253, 341, 418
NmuCI GTSAC 1 cut(s) 496
NsiI ATGCAT 1 cut(s) 652
PagI TCATGA 2 cut(s) 624, 718
PkrI GCNGC 5 cut(s) 91, 94, 313, 658, 877
PleI GAGTC 2 cut(s) 758, 993
PpsI GAGTC 2 cut(s) 758, 993
PspN4I GGNNCC 3 cut(s) 253, 341, 418
PspPI GGNCC 1 cut(s) 416
PstI CTGCAG 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 4 cut(s) 139, 386, 638, 675
RsaNI GTAC 4 cut(s) 138, 385, 637, 674
RseI CAYNNNNRTG 3 cut(s) 129, 345, 494
SaqAI TTAA 4 cut(s) 63, 329, 695, 951
SatI GCNGC 5 cut(s) 90, 93, 312, 657, 876
Sau3AI GATC 4 cut(s) 33, 222, 512, 661
Sau96I GGNCC 1 cut(s) 416
ScaI AGTACT 1 cut(s) 139
SchI GAGTC 2 cut(s) 758, 993
ScrFI CCNGG 1 cut(s) 414
SfaNI GCATC 3 cut(s) 742, 915, 931
SfcI CTRYAG 1 cut(s) 90
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 3 cut(s) 129, 345, 494
SmlI CTYRAG 1 cut(s) 612
SmoI CTYRAG 1 cut(s) 612
Sse9I AATT 6 cut(s) 154, 199, 289, 469, 847, 892
SsiI CCGC 3 cut(s) 48, 150, 677
SspMI CTAG 6 cut(s) 147, 239, 546, 570, 680, 1051
StyD4I CCNGG 1 cut(s) 412
StyI CCWWGG 2 cut(s) 162, 780
TaaI ACNGT 6 cut(s) 137, 277, 394, 442, 543, 740
TaiI ACGT 1 cut(s) 807
TaqI TCGA 2 cut(s) 171, 1005
TasI AATT 6 cut(s) 154, 199, 289, 469, 847, 892
TatI WGTACW 2 cut(s) 137, 384
Tru1I TTAA 4 cut(s) 63, 329, 695, 951
Tru9I TTAA 4 cut(s) 63, 329, 695, 951
TscAI CASTG 4 cut(s) 265, 280, 397, 1046
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 5 cut(s) 89, 92, 311, 656, 875
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 3 cut(s) 28, 461, 707
TspRI CASTG 4 cut(s) 265, 280, 397, 1046
VpaK11BI GGWCC 1 cut(s) 416
XapI RAATTY 3 cut(s) 199, 289, 892
XbaI TCTAGA 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 2 cut(s) 137, 709
XspI CTAG 6 cut(s) 147, 239, 546, 570, 680, 1051
ZrmI AGTACT 1 cut(s) 139
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.