Rh2DG499700

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0017782
Physical Location & Seq
Forward (+)
71948804 .. 71950450
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG499700.1

Sequence Viewer

Length: 777 bp
ATGAGCATATCAAATTCTGATGTGCAAAGGTTCGCTGCAAGCTTTGAAGACTTAACCAAGTGGCACCAAGATGTTTTGTATTCCTTACATATCACCTTTGATGTCAAGTACATCACTGTTGGTAACGAAGCAATCCCCGGTCCCTCTACCGATGATGTTCTGACCGTAATGAAGTCTCTACAACTGCTGAACAATTATAGCTCTTTTGGGGACATCAAGGTGACAACCTCTGTGGGATCAAGTGTTTTGGGGGATTGGTATCTACCGTCTAGTGGGGAGTTCAAACCAAATGCTAGCAGAGCCATGACTGACATACTTGGATTTCTATCAGCGTCTCAAGCACCTCTCATGATAAATGTGTACCCCTACTATGCATATGCAGCTGATCCGGCCAATGTACCGCTAGATTATGCACTCTTAAACGCCACCTACCCTATGGTTCATGATGGCTTATTGGAATACAGTAACTTGTTTGATGCAATGGTGGACTCCTTTTACTCTGCCATGGAGAAAGTTGACTACTCAGACGTGAGCATTGTGGTATCCGAGAGTGGGTGGCCTCGTGATGGGAATTACAACTTCACCACACCGGAACTTGCAGCTACATACAACAGGAATTTCCTGCTACGCATCAGCTCAAAGGTGGGAACACCCAAGAGACCTGATGCTTCTGTTAAGGGATACATATATGAGCTGTTTGATGGTAAAAGCCCTCACTTTGGACTCTTCGATGAGACCTTTGAACCTATCTATCCTCTGTTCCAGTGTTCCTTCTAG

Protein Analysis

258

Amino Acids

28.79

Weight (kDa)

4.49

Isoelectric Point (pI)

33.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 2 - 252 5.7e-48 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017782)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0150161
rosa_laevigata RLG00000020472
rosa_multiflora Rmu_co8401597.1_g000001 Rmu_sc0015510.1_g000001 Rmu_ssc0000020.1_g000012
rosa_roxburghii Rroxscaffold_2G00097390
rosa_rugosa Rorug02G0419400
rosa_samantha Rh2AG478300 Rh2BG490900 Rh2CG464500 Rh2DG499700
rosa_wichuraiana Rw2G039050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 63
AciI CCGC 1 cut(s) 401
AclWI GGATC 2 cut(s) 244, 380
AcoI YGGCCR 1 cut(s) 390
AcsI RAATTY 2 cut(s) 13, 616
AfaI GTAC 3 cut(s) 110, 362, 399
AfiI CCNNNNNNNGG 4 cut(s) 272, 552, 566, 719
AgsI TTSAA 3 cut(s) 47, 283, 743
AjiI CACGTC 1 cut(s) 529
AluBI AGCT 6 cut(s) 42, 201, 383, 602, 636, 694
AluI AGCT 6 cut(s) 42, 201, 383, 602, 636, 694
Alw26I GTCTC 4 cut(s) 180, 339, 652, 728
AlwI GGATC 2 cut(s) 244, 380
AoxI GGCC 2 cut(s) 390, 557
ApeKI GCWGC 3 cut(s) 35, 380, 599
ApoI RAATTY 2 cut(s) 13, 616
AspS9I GGNCC 1 cut(s) 140
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 3 cut(s) 85, 232, 574
AsuNHI GCTAGC 1 cut(s) 293
AvaII GGWCC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 63
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 54
BbvI GCAGC 3 cut(s) 22, 392, 611
BccI CCATC 3 cut(s) 440, 560, 695
BciVI GTATCC 2 cut(s) 553, 674
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 4 cut(s) 180, 339, 652, 728
BfaI CTAG 4 cut(s) 270, 294, 404, 775
BfuI GTATCC 2 cut(s) 553, 674
BisI GCNGC 3 cut(s) 36, 381, 600
BlsI GCNGC 3 cut(s) 37, 382, 601
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 140
BmgBI CACGTC 1 cut(s) 529
BmgT120I GGNCC 1 cut(s) 140
BmiI GGNNCC 2 cut(s) 65, 142
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 3 cut(s) 466, 639, 655
BmtI GCTAGC 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 138
BsaBI GATNNNNATC 2 cut(s) 258, 325
BsaI GGTCTC 2 cut(s) 652, 728
BsaJI CCNNGG 2 cut(s) 136, 504
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 4 cut(s) 272, 552, 566, 719
Bse1I ACTGG 1 cut(s) 763
Bse3DI GCAATG 1 cut(s) 486
Bse8I GATNNNNATC 2 cut(s) 258, 325
BseDI CCNNGG 2 cut(s) 136, 504
BseJI GATNNNNATC 2 cut(s) 258, 325
BseLI CCNNNNNNNGG 4 cut(s) 272, 552, 566, 719
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 1 cut(s) 763
BseXI GCAGC 3 cut(s) 22, 392, 611
BshFI GGCC 2 cut(s) 392, 559
BshNI GGYRCC 1 cut(s) 63
BsiSI CCGG 3 cut(s) 138, 389, 590
BslFI GGGAC 2 cut(s) 126, 224
BslI CCNNNNNNNGG 4 cut(s) 272, 552, 566, 719
BsmAI GTCTC 4 cut(s) 180, 339, 652, 728
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 2 cut(s) 126, 224
BsnI GGCC 2 cut(s) 392, 559
Bso31I GGTCTC 2 cut(s) 652, 728
Bsp143I GATC 2 cut(s) 236, 385
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 1 cut(s) 401
BspANI GGCC 2 cut(s) 392, 559
BspCNI CTCAG 1 cut(s) 536
BspHI TCATGA 2 cut(s) 348, 442
BspLI GGNNCC 2 cut(s) 65, 142
BspOI GCTAGC 1 cut(s) 297
BspPI GGATC 2 cut(s) 244, 380
BspT107I GGYRCC 1 cut(s) 63
BspTNI GGTCTC 2 cut(s) 652, 728
BsrDI GCAATG 1 cut(s) 486
BsrI ACTGG 1 cut(s) 763
BssECI CCNNGG 2 cut(s) 136, 504
BssMI GATC 2 cut(s) 236, 385
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 504
Bst2BI CACGAG 1 cut(s) 561
Bst4CI ACNGT 4 cut(s) 118, 166, 267, 464
Bst6I CTCTTC 1 cut(s) 731
BstC8I GCNNGC 2 cut(s) 40, 295
BstDEI CTNAG 1 cut(s) 523
BstDSI CCRYGG 1 cut(s) 504
BstKTI GATC 2 cut(s) 239, 388
BstMAI GTCTC 4 cut(s) 180, 339, 652, 728
BstMBI GATC 2 cut(s) 236, 385
BstMWI GCNNNNNNNGC 4 cut(s) 299, 338, 380, 389
BstSCI CCNGG 1 cut(s) 136
BstV1I GCAGC 3 cut(s) 22, 392, 611
BstV2I GAAGAC 1 cut(s) 54
BsuI GTATCC 2 cut(s) 553, 674
BsuRI GGCC 2 cut(s) 392, 559
BtgI CCRYGG 1 cut(s) 504
BtrI CACGTC 1 cut(s) 529
BtsIMutI CAGTG 2 cut(s) 114, 770
Cac8I GCNNGC 2 cut(s) 40, 295
CciI TCATGA 2 cut(s) 348, 442
Cfr13I GGNCC 1 cut(s) 140
CseI GACGC 1 cut(s) 321
Csp6I GTAC 3 cut(s) 109, 361, 398
CspCI CAANNNNNGTGG 2 cut(s) 213, 248
CviAII CATG 4 cut(s) 304, 349, 443, 505
CviQI GTAC 3 cut(s) 109, 361, 398
DdeI CTNAG 1 cut(s) 523
DpnI GATC 2 cut(s) 238, 387
DpnII GATC 2 cut(s) 236, 385
EaeI YGGCCR 1 cut(s) 390
Eam1104I CTCTTC 1 cut(s) 731
EarI CTCTTC 1 cut(s) 731
Eco130I CCWWGG 1 cut(s) 504
Eco31I GGTCTC 2 cut(s) 652, 728
Eco47I GGWCC 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 504
EcoT22I ATGCAT 1 cut(s) 376
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 4 cut(s) 307, 352, 446, 508
FaqI GGGAC 2 cut(s) 126, 224
FatI CATG 4 cut(s) 303, 348, 442, 504
FauNDI CATATG 1 cut(s) 376
Fnu4HI GCNGC 3 cut(s) 36, 381, 600
Fsp4HI GCNGC 3 cut(s) 36, 381, 600
FspBI CTAG 4 cut(s) 270, 294, 404, 775
GluI GCNGC 3 cut(s) 36, 381, 600
HaeIII GGCC 2 cut(s) 392, 559
HapII CCGG 3 cut(s) 138, 389, 590
HgaI GACGC 1 cut(s) 321
Hin1II CATG 4 cut(s) 307, 352, 446, 508
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 2 cut(s) 488, 723
HpaII CCGG 3 cut(s) 138, 389, 590
HphI GGTGA 3 cut(s) 85, 232, 574
Hpy166II GTNNAC 3 cut(s) 361, 487, 517
Hpy188I TCNGA 4 cut(s) 19, 162, 526, 547
Hpy188III TCNNGA 3 cut(s) 349, 443, 563
Hpy8I GTNNAC 3 cut(s) 361, 487, 517
HpyCH4III ACNGT 4 cut(s) 118, 166, 267, 464
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 7 cut(s) 25, 38, 374, 380, 413, 479, 599
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 338, 380, 389
HpyF3I CTNAG 1 cut(s) 523
HpySE526I ACGT 1 cut(s) 528
Hsp92II CATG 4 cut(s) 307, 352, 446, 508
Kzo9I GATC 2 cut(s) 236, 385
LpnPI CCDG 6 cut(s) 151, 402, 598, 603, 635, 675
Lsp1109I GCAGC 3 cut(s) 22, 392, 611
LweI GCATC 3 cut(s) 466, 639, 655
MaeI CTAG 4 cut(s) 270, 294, 404, 775
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 3 cut(s) 122, 220, 464
MalI GATC 2 cut(s) 238, 387
MboI GATC 2 cut(s) 236, 385
MboII GAAGA 2 cut(s) 59, 718
MluCI AATT 4 cut(s) 13, 193, 571, 616
MlyI GAGTC 2 cut(s) 482, 717
MnlI CCTC 6 cut(s) 154, 238, 354, 570, 723, 765
Mph1103I ATGCAT 1 cut(s) 376
MseI TTAA 3 cut(s) 53, 419, 675
MslI CAYNNNNRTG 2 cut(s) 69, 218
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 3 cut(s) 138, 389, 590
MspR9I CCNGG 1 cut(s) 138
MwoI GCNNNNNNNGC 4 cut(s) 299, 338, 380, 389
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 1 cut(s) 504
NdeI CATATG 1 cut(s) 376
NdeII GATC 2 cut(s) 236, 385
NheI GCTAGC 1 cut(s) 293
NlaIII CATG 4 cut(s) 307, 352, 446, 508
NlaIV GGNNCC 2 cut(s) 65, 142
NmuCI GTSAC 1 cut(s) 220
NsiI ATGCAT 1 cut(s) 376
PagI TCATGA 2 cut(s) 348, 442
PkrI GCNGC 3 cut(s) 37, 382, 601
PleI GAGTC 2 cut(s) 482, 717
PpsI GAGTC 2 cut(s) 482, 717
PspN4I GGNNCC 2 cut(s) 65, 142
PspPI GGNCC 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 3 cut(s) 110, 362, 399
RsaNI GTAC 3 cut(s) 109, 361, 398
RseI CAYNNNNRTG 2 cut(s) 69, 218
SaqAI TTAA 3 cut(s) 53, 419, 675
SatI GCNGC 3 cut(s) 36, 381, 600
Sau3AI GATC 2 cut(s) 236, 385
Sau96I GGNCC 1 cut(s) 140
SchI GAGTC 2 cut(s) 482, 717
ScrFI CCNGG 1 cut(s) 138
SfaNI GCATC 3 cut(s) 466, 639, 655
SinI GGWCC 1 cut(s) 140
SmiMI CAYNNNNRTG 2 cut(s) 69, 218
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
Sse9I AATT 4 cut(s) 13, 193, 571, 616
SsiI CCGC 1 cut(s) 401
SspMI CTAG 4 cut(s) 270, 294, 404, 775
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 4 cut(s) 118, 166, 267, 464
TaiI ACGT 1 cut(s) 531
TaqI TCGA 1 cut(s) 729
TasI AATT 4 cut(s) 13, 193, 571, 616
TatI WGTACW 1 cut(s) 108
Tru1I TTAA 3 cut(s) 53, 419, 675
Tru9I TTAA 3 cut(s) 53, 419, 675
TscAI CASTG 2 cut(s) 121, 770
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 3 cut(s) 35, 380, 599
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 2 cut(s) 185, 431
TspRI CASTG 2 cut(s) 121, 770
VpaK11BI GGWCC 1 cut(s) 140
XapI RAATTY 2 cut(s) 13, 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XspI CTAG 4 cut(s) 270, 294, 404, 775
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.