RchiOBHm_Chr1g0328041

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0020412
Physical Location & Seq
Reverse (-)
17271309 .. 17272568
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 381 bp
ATGAAGTTTTTGTGGGTTGGCATTGTTGTAGTGCTAGTTTTCACCAAATGGGCTAATGGGTTTGAGGAGCATAGTTTTAATGAAACAGAGCTGTCCTGGTTGGAGGGCTATGGAGAATCCAAGGCTGCTGTAACTGGGGTGATGGTAGGACTAACCCTTATTCCAGGAGCTGGTGCCAAAGGAGCAGTCTGCCTCGATGGAACCTTACCCGGGTATCATTTGCATCGTGGGTACGGGTCAGGAGCAAATAGCTGGCTTATTCAATTGGAGGGGGGAGGATGGTGTAACACCATTAGAAATTGTGCTTACCGCAAAACAACAAGGCGGGGATCATCAAAATTTATGGAAAAACAGTTGCTATTTACCGGAATACAAAGCTGA

Protein Analysis

126

Amino Acids

13.76

Weight (kDa)

8.86

Isoelectric Point (pI)

17.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 45 - 126 1.1e-34 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020412)

Species Orthologous Gene IDs
prunus_persica Prupe.6G303100_v2.0.a1
pyrus_communis pycom04g16370
rosa_chinensis RchiOBHm_Chr1g0328041
rosa_roxburghii Rroxscaffold_4G00289790
rosa_samantha Rh5AG130300
rosa_wichuraiana Rw5G016610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AccB7I CCANNNNNTGG 1 cut(s) 170
AciI CCGC 2 cut(s) 310, 325
AclWI GGATC 1 cut(s) 337
AcsI RAATTY 1 cut(s) 338
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 2 cut(s) 170, 210
AgsI TTSAA 1 cut(s) 263
AjnI CCWGG 2 cut(s) 95, 163
AluBI AGCT 4 cut(s) 91, 170, 252, 378
AluI AGCT 4 cut(s) 91, 170, 252, 378
AlwI GGATC 1 cut(s) 337
AlwNI CAGNNNCTG 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 125
ApoI RAATTY 1 cut(s) 338
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AsuC2I CCSGG 2 cut(s) 210, 211
AsuHPI GGTGA 2 cut(s) 34, 151
AvaI CYCGRG 1 cut(s) 209
BaeI ACNNNNGTAYC 2 cut(s) 197, 230
BanI GGYRCC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 112
BccI CCATC 3 cut(s) 136, 191, 273
BciT130I CCWGG 2 cut(s) 97, 165
BcnI CCSGG 2 cut(s) 210, 211
BfaI CTAG 1 cut(s) 35
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 4 cut(s) 97, 165, 210, 211
BmeT110I CYCGRG 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 175, 202
BmrFI CCNGG 4 cut(s) 97, 165, 210, 211
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 232
BmuI ACTGGG 1 cut(s) 144
BpuMI CCSGG 2 cut(s) 210, 211
BsaJI CCNNGG 2 cut(s) 120, 209
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 210
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 2 cut(s) 97, 165
BseDI CCNNGG 2 cut(s) 120, 209
BseGI GGATG 1 cut(s) 284
BseLI CCNNNNNNNGG 2 cut(s) 170, 210
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 112
BshNI GGYRCC 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 2 cut(s) 210, 366
BslI CCNNNNNNNGG 2 cut(s) 170, 210
BsoBI CYCGRG 1 cut(s) 209
Bsp143I GATC 1 cut(s) 329
BspACI CCGC 2 cut(s) 310, 325
BspLI GGNNCC 2 cut(s) 175, 202
BspPI GGATC 1 cut(s) 337
BspT107I GGYRCC 1 cut(s) 173
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 2 cut(s) 120, 209
BssMI GATC 1 cut(s) 329
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 2 cut(s) 97, 165
Bst4CI ACNGT 1 cut(s) 354
BstC8I GCNNGC 1 cut(s) 254
BstF5I GGATG 1 cut(s) 284
BstKTI GATC 1 cut(s) 332
BstMBI GATC 1 cut(s) 329
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 2 cut(s) 97, 165
BstSCI CCNGG 4 cut(s) 95, 163, 208, 209
BstV1I GCAGC 1 cut(s) 112
BtsCI GGATG 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 254
CaiI CAGNNNCTG 1 cut(s) 170
Cfr9I CCCGGG 1 cut(s) 209
Csp6I GTAC 1 cut(s) 232
CviJI RGCY 8 cut(s) 53, 91, 108, 125, 170, 252, 256, 378
CviKI_1 RGCY 8 cut(s) 53, 91, 108, 125, 170, 252, 256, 378
CviQI GTAC 1 cut(s) 232
DpnI GATC 1 cut(s) 331
DpnII GATC 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 209
EcoRII CCWGG 2 cut(s) 95, 163
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaiI YATR 3 cut(s) 72, 111, 344
FauI CCCGC 1 cut(s) 318
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 1 cut(s) 291
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 1 cut(s) 35
GluI GCNGC 1 cut(s) 126
HapII CCGG 2 cut(s) 210, 366
HinfI GANTC 1 cut(s) 116
HpaII CCGG 2 cut(s) 210, 366
HphI GGTGA 2 cut(s) 34, 151
Hpy188III TCNNGA 1 cut(s) 240
HpyCH4III ACNGT 1 cut(s) 354
HpyCH4V TGCA 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
Kzo9I GATC 1 cut(s) 329
LmnI GCTCC 4 cut(s) 67, 167, 182, 242
LpnPI CCDG 9 cut(s) 82, 109, 120, 150, 156, 177, 223, 225, 238
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 1 cut(s) 232
MaeI CTAG 1 cut(s) 35
MaeIII GTNAC 2 cut(s) 130, 284
MalI GATC 1 cut(s) 331
MboI GATC 1 cut(s) 329
MfeI CAATTG 1 cut(s) 263
MluCI AATT 3 cut(s) 263, 298, 338
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 5 cut(s) 58, 97, 203, 262, 269
MseI TTAA 1 cut(s) 78
MspI CCGG 2 cut(s) 210, 366
MspR9I CCNGG 4 cut(s) 97, 165, 210, 211
MunI CAATTG 1 cut(s) 263
MvaI CCWGG 2 cut(s) 97, 165
MwoI GCNNNNNNNGC 1 cut(s) 182
NciI CCSGG 2 cut(s) 210, 211
NdeII GATC 1 cut(s) 329
NlaIV GGNNCC 2 cut(s) 175, 202
PfeI GAWTC 1 cut(s) 116
PflMI CCANNNNNTGG 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 163
PkrI GCNGC 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 95, 163
PspGI CCWGG 2 cut(s) 95, 163
PspN4I GGNNCC 2 cut(s) 175, 202
PstNI CAGNNNCTG 1 cut(s) 170
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 1 cut(s) 329
ScrFI CCNGG 4 cut(s) 97, 165, 210, 211
SetI ASST 5 cut(s) 93, 172, 206, 254, 380
SfaNI GCATC 1 cut(s) 232
SmaI CCCGGG 1 cut(s) 211
Sse9I AATT 3 cut(s) 263, 298, 338
SsiI CCGC 2 cut(s) 310, 325
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 4 cut(s) 95, 163, 208, 209
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 1 cut(s) 354
TaqI TCGA 1 cut(s) 195
TasI AATT 3 cut(s) 263, 298, 338
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseI GCWGC 1 cut(s) 125
TspDTI ATGAA 2 cut(s) 17, 96
TspMI CCCGGG 1 cut(s) 209
Van91I CCANNNNNTGG 1 cut(s) 170
XapI RAATTY 1 cut(s) 338
XmaI CCCGGG 1 cut(s) 209
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.